LOCUS       CP058001                4091 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB06-C03 plasmid pRHB06-C03_6, complete
            sequence.
ACCESSION   CP058001
VERSION     CP058001.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148008
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH14 T1-C03
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 89.5791523127404x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 13:55:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,950
            CDSs (total)                      :: 4,831
            Genes (coding)                    :: 4,722
            CDSs (with protein)               :: 4,722
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 51 of 109
            Pseudo Genes (incomplete)         :: 40 of 109
            Pseudo Genes (internal stop)      :: 32 of 109
            Pseudo Genes (multiple problems)  :: 14 of 109
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB06-C03"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB06-C03_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(3044..4091,1..1100)
                     /locus_tag="HVV91_24730"
     CDS             join(3044..4091,1..1100)
                     /locus_tag="HVV91_24730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QMQ11818.1"
                     /translation="MAIYHLSMKILSRSKGYSAVASAAYRAGEKILDERTGVIHDYTRK
                     NGVASAVILTPANAPAWCANRAELWNAVEKAERRKNSQLAREFELAIPRELAQDAARET
                     VLNFVRENFVSRGMIADVAFHNLGGSNPHAHIMLSMRAITPDGFGEKVREWNDWTHAET
                     WRGSWADHANRALANAGYQEEIDHRSYERQGVEKTPGIHLGKSACAMEKWGIETERGEQ
                     NRLINRLNLEIQVSRDELRNSELTEPARRMADLLNIELPDNATSYILHDIIEALPTDSN
                     AAWKLTSKAMSMMADMQATRRRWEDLNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWV
                     APEYGREQEKIQSLSQRMEKLRQHHWKVEKQDIPASQKAFETQWQQWGASGLADLREQL
                     RKREEELRRKEQEETERRRAEQLRRNDNAVMDSGVLKAVVTGYGEAPMPGNGEMTRYLL
                     LHNRNGEYTLWGNELEKYRARIFESVDLMRDRSGYICERSEIGQRPPLQHVYSSATFEQ
                     LLTQVCQTWPQHTRDLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSMGGVVRLNGTLYPAFAVPTADNSQLVINALTDKGLRFA
                     GYGEAVNHDADSPARPAPELMQFHLKTWSEPLFAIVNTPEKQPDHLFRSLGFERTWDEW
                     QRDEYARTHATERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     gene            complement(1349..2050)
                     /locus_tag="HVV91_24735"
     CDS             complement(1349..2050)
                     /locus_tag="HVV91_24735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QMQ11819.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGSDAESTEL
                     QKTMTVTAEEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCVDELNQKSDLAVTLETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(2583..2873)
                     /locus_tag="HVV91_24740"
     CDS             complement(2583..2873)
                     /locus_tag="HVV91_24740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QMQ11820.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     GETQLRAWLLHLLKTEVTRQADMSRIQPLIDELNALPKPVPKEVSENDQQA"
                     /name="mobilization protein"
     Typing          complement(2878..3028)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.81"
                     /coverage="85.0"
                     /evalue="3.53e-55"
     Typing          complement(3045..3399)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1351..2050)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.301"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4..1097
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.452"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3003..4090
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.725"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aaatccagtc gttgagccag cgcatggaaa aattgcggca gcatcactgg aaggttgaga
       61 aacaggacat tcccgcgagt caaaaagcat ttgaaacaca gtggcagcag tggggtgcat
      121 ctggccttgc agatctccga gaacagttgc gtaaacgtga agaggaattg cgccggaagg
      181 agcaggagga aacagaacgt cgtcgggcgg agcagctcag gagaaatgac aatgccgtta
      241 tggattcagg ggtactgaaa gcagtcgtta cggggtatgg cgaggccccc atgccaggta
      301 acggggaaat gacccgttac cttctgcttc acaaccggaa cggagaatat acgctctggg
      361 ggaacgaact ggagaaatac cgcgccagaa tttttgagag tgttgattta atgcgtgaca
      421 ggagtggtta tatctgtgag cgaagcgaaa tcggtcaacg tccaccactg caacatgttt
      481 attcctctgc gacctttgag caactgctga cgcaggtttg ccagacatgg ccgcaacata
      541 cccgcgatct ccgccagccg aagacatggc cggagtcttt ctgtcttgga gaagaccgcc
      601 agccagccat gccatcactg gcggcaagga aggtggattt tacgcaggga cgtctgccac
      661 cgactctgat gccggtaatg agttctgtcg accgtgaaac ccgccagctt cagcttttgc
      721 tggtcatggg tgtggatgac tctatgggcg gtgttgtccg gcttaatggt acgctgtatc
      781 ccgcgtttgc cgtccccaca gccgacaaca gccagctggt aatcaatgca ctgaccgaca
      841 aaggtttacg ctttgcaggc tatggtgaag cggtaaacca cgatgctgac agtccggcac
      901 gtcctgcgcc ggaacttatg cagtttcatc tgaaaacctg gagcgaaccg ctttttgcta
      961 tcgtcaacac gccagaaaaa cagccggatc atctgttccg ctcgctgggc tttgagcgca
     1021 cctgggatga atggcaacgt gatgaatatg cccgaacaca cgccaccgag aggagacatg
     1081 atcgcggttg gtcacagtga atgtgaccaa tattttgttt tgtttaacat cgtgttatgt
     1141 tcgtcgatgt tcatcgacag ggatgaatgc aagaagcaca cacataacac aaatcaactt
     1201 tatagttttc ccgcccccga aaaattgggg gttctttttt cactgtgcca gagcgtttat
     1261 gacgcaattt tttacttcac cagtaccttc gcatgccaaa cacaaaaaaa acgcgtcaga
     1321 gagcgtttta gaggccttaa aatcgattct agatcgtcat cttgatctgt ttgtcttcct
     1381 tgaagcgaaa atgcagggct acaaccgtgc gccccttctt gatcgtctca agcgttacag
     1441 ccaagtcgct tttttgattc agttcgtcaa cgcaaggtct taataattgc tggttcagag
     1501 acttatatgt ttgatattta tgctctatat caagcattga gcgaaaatca tcgagggcga
     1561 taatacgttc ccctgtgctg cggaattgct ggagaagttc gtatattctt atgctgtaac
     1621 ttcttgataa attggagatg ttcttaatta caactctagt aaattgcccc ttcaactgtg
     1681 tcagataagg catgaccgca tctgaaaaag tgatttgggc tttgccctca cctttagcgt
     1741 actgtgcccg atactggatc cagcggaact cctcgcgctt ctcatcgtcc ttaaggatga
     1801 tcgacctgtc ccacaaacga tcaattgctt cctgtagctg aacctcggca tttttccgcc
     1861 ccatatcagg gaatgagtcg aaatactctt cggcggtaac agtcatggtc ttttgcaatt
     1921 cagtggattc tgcatcagaa cccgacttca atcgaccaat gcaaagtaac aagaatcgct
     1981 gttcctgaag agtcaattta tagctggcct caaccagtgc attggatttg taggcaatca
     2041 gatccgacat tgtaaatccc tcaacctaag gtaatgagta aaatataaac attcctttag
     2101 gtaaaggcaa tatgatttaa ttttaatatc ccttttcctg tgaatattac cttaaatcat
     2161 attgccttta ccttaaatca tattgcctat ccaccttaaa tcatattgcc tttaccttaa
     2221 attatattac ctatagtctt ctaaactctt gaatttcata agaaaattcg accctaaaac
     2281 aagtattaaa aacaagatct taaaaacaag atctaaaaac ttaacggcga gcctgtggaa
     2341 aacttggggt gttcaaaaca aaaaatcatg aaaaacaaca ggttacaaaa aagcaggttc
     2401 gcaagcttta gcaaaacccc gctcaaccct gtggaaatcc agtattcatg cgggttcagc
     2461 gcatatcacc tgtcgaaaaa ccgtgcgtat gtgctcaatc tggcgacgga ttcacttccg
     2521 cttcctcgcc cactcgctcg ctaacgctcg gtcgctgggg ctgcggcgag cggacggtgc
     2581 attcacgcct gttggtcgtt ttctgacact tctttgggga ctggttttgg taacgcgttc
     2641 agttcgtcaa tcaacggctg tatcctgctc atatcagcct gccgtgttac ctctgtcttc
     2701 aggagatgaa gcaaccaggc tcgtagttgt gtttccccag gcttcctgac ctttgccagc
     2761 aatgccgctc ccacaatgac cttctgcgct gtgtccaggc tcctgctctt cgccttcaga
     2821 cgctgcaatc tggcctcagc atcggcaatc ttctgttcga gtgttctact cattccgtaa
     2881 ctccgtgcgc ggtgaaaaat cgcattttag cgcgtcactg gtagtttaaa aactgaactg
     2941 gcataatgca cagcaaatca cgaagtgcgc acttatacaa actctccttc gtcgagtttg
     3001 tgtgcgccct gcggggctaa aagaaaacgg caacaacgac acgatggcaa tttatcatct
     3061 cagcatgaag attctttcca gaagcaaggg ctacagcgcc gttgcttctg ctgcctatcg
     3121 tgccggagaa aaaatactgg atgagcgcac cggagtgatt cacgactaca cccgcaaaaa
     3181 cggtgtagcc agcgcagtga ttctgacgcc agcaaacgcc ccagcatggt gtgcgaaccg
     3241 cgccgaactc tggaatgctg ttgaaaaagc cgagcggcgg aaaaactcac aacttgcgcg
     3301 ggagttcgaa ctcgccatcc cccgtgaact ggcgcaggat gccgcacggg aaacagttct
     3361 caacttcgta agggaaaact ttgtcagtcg cggcatgatc gccgacgtgg cctttcacaa
     3421 tctgggcggg tcaaacccac acgcgcacat catgctatcc atgagggcaa tcacgcctga
     3481 cggcttcggg gagaaagtcc gtgagtggaa tgactggact cacgccgaaa catggcgggg
     3541 atcatgggca gaccatgcga acagggcgct ggcgaacgcc ggttatcagg aagagataga
     3601 ccatcgttca tacgagcgac agggggttga gaaaacgcct ggcattcacc tcggaaagtc
     3661 ggcctgtgcg atggaaaagt ggggcatcga aacagaacgc ggcgaacaga atcgcctgat
     3721 taacaggctt aacctggaaa tacaggtttc ccgcgacgaa ctcaggaaca gtgagctgac
     3781 agagcccgca cgccgtatgg ctgaccttct gaatattgag cttcctgaca acgcaacttc
     3841 gtacatactg catgacatca tcgaagcgtt gccgacggac agtaacgcag catggaaact
     3901 caccagtaaa gccatgtcga tgatggcaga tatgcaggcg acgcgcagac gctgggaaga
     3961 tctgaacacc gaacgaaagg aagcgatgag ccatgctgcc agcctgaaaa aatcatcgcc
     4021 gttttcgtcc ggtttttccc gtattcccct gatgcaatgg gttgcaccgg aatacggccg
     4081 cgaacaggaa a
//
