LOCUS       CP057956                1306 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB08-C23 plasmid pRHB08-C23_4, complete
            sequence.
ACCESSION   CP057956
VERSION     CP057956.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148041
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH06 T1-C23
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.9459087646926x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 13:22:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,004
            CDSs (total)                      :: 4,887
            Genes (coding)                    :: 4,714
            CDSs (with protein)               :: 4,714
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 65 of 173
            Pseudo Genes (incomplete)         :: 91 of 173
            Pseudo Genes (internal stop)      :: 50 of 173
            Pseudo Genes (multiple problems)  :: 30 of 173
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB08-C23"
                     /isolation_source="Pooled cattle faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB08-C23_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(440..976)
                     /locus_tag="HVW24_25020"
     CDS             complement(440..976)
                     /locus_tag="HVW24_25020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QMP09202.1"
                     /translation="MQTQNVITEKEREIRSRDLTENAKKLAHERREAEKNSNFTQVSPL
                     GWKRLMELAKGNQGAFGLYSFFAQHIDGSCGAVVADQSFLAAQFNVSTRTIIRWINYLE
                     ENRAVVRIPVAGKVCAYALNPNEVWKGYGNTKEYAAFITKTLVNKDGDIQRRIMAMFHG
                     NDARQGMEGDTPEAE"
                     /name="helix-turn-helix domain-containing protein"
ORIGIN
        1 gttgccaacg caccggtcga aaaaaatagg ggaaggtggt tgtgttcacg cagtgagcgc
       61 aaccacctgc cagacgggtt ttcgcgccgc aggccgcaca tgtttctgag ccaacggcga
      121 agaaacatat aagtgcgcat ctctctaatt ttgcgaattt gagagatgaa accagtctgg
      181 gggatcggag tattccgcct ctagccgttc tggtggccca gtccggcaag acccacgaaa
      241 tcacccgtaa aacgccctaa aacgcgctct gacgcgtttt ttgacgtttt acgggctcac
      301 gtgggctttg ggcggaaaaa tggcttacgt gcgttctgag agcgttttag ggctttgcag
      361 ggcggagggg gtgaagcagc tccctgtgga acctgtaccg cccttgcggg cgggcttcgg
      421 cgatttggcg aacgggaatc tattcggctt ctggcgtgtc gccttccatg ccctgacggg
      481 catcatttcc gtggaacatg gccatgattc ggcgctgaat gtcgccgtct ttgttgacta
      541 gggtttttgt tataaacgcc gcatattcct tggtattccc ataccccttc caaacttcat
      601 ttggattcaa tgcgtatgca caaaccttac cggcgacagg aatcctcaca acagccctgt
      661 tttcctctaa atagttgatc catctaatta ttgttcttgt cgatacattg aactgcgccg
      721 ccaaaaatga ctgatccgcc actactgcgc cacaagatcc atcgatatgc tgagcaaaaa
      781 aagaatacaa gccaaaagct ccctgatttc ctttggctaa ttccattaaa cgcttccagc
      841 ctaatgggga cacttgtgtg aaatttgagt tcttttctgc ctctcgcctc tcatgagcca
      901 gttttttggc attttcggtt agatccctag atcgaatttc tcgctctttt tcggtgatta
      961 cattttgagt ttgcatctca atctccatgt catttcaatg tgacaagata agtcatacta
     1021 tacgacattt tatgtcgtag tatatgtcat ttatgtcaca tattacgaaa tgatgtgaca
     1081 tcctagggtg tcataacgtt gtgacatcct agggtgtcat atcagaaatg ttaaatgtct
     1141 tttaaatcaa tgaattatta atttttgcta ttcttatcta aagagatctc acagcgcgct
     1201 gcgagatctc ctggtagcac cctcctttca gtcgggcaaa cggcgaaaat ttaaccattg
     1261 gcaaccgaaa aatcgtgggg gccccttcag gggttccgat tttttt
//
