LOCUS       CP057934                6811 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB09-C20 plasmid pRHB09-C20_3, complete
            sequence.
ACCESSION   CP057934
VERSION     CP057934.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148050
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6811)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6811)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH08 T1-C20
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 71.3022014516544x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 12:39:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,022
            CDSs (total)                      :: 4,903
            Genes (coding)                    :: 4,774
            CDSs (with protein)               :: 4,774
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 53 of 129
            Pseudo Genes (incomplete)         :: 70 of 129
            Pseudo Genes (internal stop)      :: 41 of 129
            Pseudo Genes (multiple problems)  :: 33 of 129
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB09-C20"
                     /isolation_source="Pooled cattle faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB09-C20_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(6793..6811,1..116)
                     /locus_tag="HVW33_25020"
                     /pseudo=""
     CDS             join(6793..6811,1..116)
                     /locus_tag="HVW33_25020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /name="colicin release lysis protein"
     gene            complement(1139..1330)
                     /locus_tag="HVW33_25025"
     CDS             complement(1139..1330)
                     /locus_tag="HVW33_25025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMO81336.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1394..1717
                     /locus_tag="HVW33_25030"
     CDS             1394..1717
                     /locus_tag="HVW33_25030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMO81344.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1659..2417
                     /locus_tag="HVW33_25035"
                     /pseudo=""
     CDS             1659..2417
                     /locus_tag="HVW33_25035"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2395..2913
                     /locus_tag="HVW33_25040"
     CDS             2395..2913
                     /locus_tag="HVW33_25040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QMO81337.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            2920..3153
                     /gene="mbeD"
                     /locus_tag="HVW33_25045"
     CDS             2920..3153
                     /gene="mbeD"
                     /locus_tag="HVW33_25045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMO81338.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="mbeD"
     gene            complement(3509..3679)
                     /locus_tag="HVW33_25050"
     CDS             complement(3509..3679)
                     /locus_tag="HVW33_25050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005074609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMO81339.1"
                     /translation="MSKAPIGTTASTGQKCPESGVWKVVGTPSTTAPIAKGNVMPPYNG
                     KAVTWKLTQYA"
                     /name="hypothetical protein"
     gene            complement(3873..4292)
                     /gene="exc2"
                     /locus_tag="HVW33_25055"
     CDS             complement(3873..4292)
                     /gene="exc2"
                     /locus_tag="HVW33_25055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMO81340.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSQSDVQQRIQHFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(4373..4810)
                     /locus_tag="HVW33_25060"
     CDS             complement(4373..4810)
                     /locus_tag="HVW33_25060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QMO81341.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGFVSYRTSQDGRR
                     EFETSELIRAYGELKQNETPERHSAGQAENPHDQPTERILRELNELKQCLTLMLEDKQA
                     QDMDRKRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            5069..6232
                     /gene="cna"
                     /locus_tag="HVW33_25065"
     CDS             5069..6232
                     /gene="cna"
                     /locus_tag="HVW33_25065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin colicin N"
                     /protein_id="QMO81342.1"
                     /translation="MGSNGADNAHNNAFGGGKNPGIGNTSGAGSNGSASSNRGNSNGWS
                     WSNKPHKNDGFHSDGSYHITFHGDNNSKPKPGGNSGNRGNNGDGASAKVGEITITPDNS
                     KPGRYISSNPEYSLLAKLIDAESIKGTEVYTFHTRKGQYVKVTVPDSNIDKMRVDYVNW
                     KGPKYNNKLVKRFVSQFLLFRKEEKEKNEKEALLKASELVSGMGDKLGEYLGVKYKNVA
                     KEVANDIKNFHGRNIRSYNEAMASLNKVLANPKMKVNKSDKDAIVNAWKQVNAKDMANK
                     IGNLGKAFKVADLAIKVEKIREKSIEGYNTGNWGPLLLEVESWIIGGVVAGVAISLFGA
                     VLSFLPISGLAVTALGVIGIMTISYLSSFIDANRVSNINNIISSVIR"
                     /name="cna"
     gene            complement(6241..6765)
                     /gene="cni"
                     /locus_tag="HVW33_25070"
     CDS             complement(6241..6765)
                     /gene="cni"
                     /locus_tag="HVW33_25070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_061352107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin N immunity protein"
                     /protein_id="QMO81343.1"
                     /translation="MDIKDRNKISKKISFSLLLLLSPFALIFFSYNNAPIPLLEKIIAY
                     LSLPGFHSLNNPPLSEAFNLYVHTAPLAAISLFIFTHKELELKPKSSPLRALKILTPFT
                     ILYISMIYCFLLTDTELTLSSKTFVLMSKNDLFLSFFYITLYIGIYIFTYLYFWFLIGT
                     YKLFTRGGILP"
                     /name="cni"
     Typing          967..1054
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          complement(398..943)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.526"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1708..3257
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.183"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taatcttgtt ttttatcatg acacttagtg catgtcaggt caatcacatc cgtgatgtga
       61 aaggagggac tgttgctcca tcatcatcat ccagactaac aggggttgaa attaagtaaa
      121 agatcaaagg atcctcttga gatccttttt ttctacgcgt aatctgctgc ctgtaaacga
      181 aaaaaccgcc tggggaggcg gtttagtcga aggttaagtc agttggggac tgcttaaccg
      241 aggtaactgg cttcagcaga gcgcagatac caaatactgt cctttcagtg tagccgcagt
      301 tgggccatca cttcaagaac tctgtaagca tctggataaa tctcgctctg ctaatccggt
      361 taccagtggc tgctgccagt ggcgttaagg cgtgtcttac cgggttggac tcaagacgat
      421 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      481 tggagcgaac gacctacacc aagccgagat accaacagcg tgagctatga gaaagcgcca
      541 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      601 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      661 gccacctctg acttgagtgt cggtttttgt gatgctcgtc aggggggcgg agcctatgga
      721 aaaacgccat cggcgcggcc tttttccggt tcctggtctt ttgctcacat gttctttccg
      781 gccttatccc ctgattctgt ggataaccgt attaccgtct ttgagtgagc tgataccgct
      841 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
      901 atgcggtatg ttctccttac gcatctgtgc ggcatttcac acccggcatg gcgtgctttt
      961 catacaatcc gcactgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     1021 actgggtcag ggctgcgccc cgacacccgc taaaacctgc tgacgcgccc tgacgggctt
     1081 gtctgctccc ggcatccgct cacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     1141 agaggttttc accgtcatcc ccgaaacgtg cgaggcagct gcggtaaagc tcatcggcgt
     1201 ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagttcg ttgagctttt
     1261 ccagcagcgt taatgtctgg cttctgataa agcgcgccat gttaagggcg gttttttcct
     1321 gtttagtcac tgatgcctcc gtgtaagggg gatttctgtt catggggtaa tgataccgat
     1381 gaaacgcgag aggatgctca cgatacgggt tactgatgat gaacatgcca ggttacttga
     1441 acgctgtgag ggtaaacaac tggcagtatg gatgcggcgg gtctgcctgg gtgagccggt
     1501 tgcccgttcc gggaagctgc cgacactggc accgccgtta ctgcgtcagc tggccgccat
     1561 cgggaataac ctgaatcaga cagcgaggaa ggtgaacagc gggcagtggt cttccggtga
     1621 ccgggttcag gtggtggccg cactgatggc catcggggat gagctgcgcc ggctgcgtct
     1681 ggccgtcagg gaacaggggg cgcgggatga tagttaaatt tcatgccagg ggaaaaggtg
     1741 gtggcagtgg tccggttgat tacctgctgg ggagggagcg taaccgcgaa ggcgcaacgg
     1801 tgcttcaggg taatccggaa gaagtccggg aactcatcga tgccacgcca tttgcgaaga
     1861 aatacacgtc cggtgttctg tcgttcgcgg agaaggagct gccgccggga ggacgtgaaa
     1921 aagtgatggc gagctttgag cgtgtactga tgcccggtct cgaaaaaaat cagtacagca
     1981 tcctgtgggt ggagcaccag gacaagggac ggcttgagct gaattttgtc attccgaaca
     2041 tggagctaca gaccggaaaa cgcctccagc cgtactacga ccgcgcagac aggcctagaa
     2101 ttgatgcctg gcagacgctg gttaatcacc attacgggct gcatgacccg aacgccccgg
     2161 agaaccgcag gacgctgaca ctctctgata atctgccgga aacgaaacag gcgattgctg
     2221 agggcgttac acgcgggata gacgcgcttt accatgccgg agagataaaa ggccgtcagg
     2281 atgtgattca ggcgctcaca gaggcggggc ttgaagtggt cagggtgacg cgaagcagta
     2341 tcagcattgc agacccgaac ggcgggagga atatcagact gaaaggagcg ttttatgagc
     2401 aatcttttac agacgggcgc ggagttcgag aaaaagctga aagagagagc cgaatctaca
     2461 gagaaaatgc tgaacgacga gttcaggaag ctcggcgagt ctgtcagcga ggctgtgacc
     2521 tcaaacgaga cgaaaatcag agacgctata gccctgttca cagcctcgac agagaaatcg
     2581 ctggaaaaac accgggaagg ggtgaaagag gcgatgatgc agcacaggaa ggacgtgtta
     2641 aagctggcag ggaatacggg catgatgtta ctggggatag tctttctcct gtttaccgcg
     2701 agtggcggga cgctctggta tcttggcgga cggatacaga cgaacctgga agaaatcagg
     2761 aagcaggaag agacattgca gaaactgaac gcgaagacat ggggcgtgga gtttgtgcag
     2821 gacgggaaca ggaaattcct tgtccttccg tacgggaaat cagcggaggt gattcccttt
     2881 caggggaaag agtgggtaca tctgaaggag tgacacagag tgacagagct ggaaatacat
     2941 ttgctgaacg ccttagagca gctgcaacag gactatatgc agcggctgag cgaatgggag
     3001 agcgccttcg tggaattgca gaagatgttt tcgcttacgc aacgggacaa cgcgatgctg
     3061 aacgagcggg tcatgcagtt gagtcagcag gtgcagcact tgagcgagca gacagaacgc
     3121 ttgagccagt tatacagcga gaactggaga taagagagga acggctgata caggagcgcg
     3181 aacatgcgtt atccctggaa cgggagcgtc agccggaaat acaggaacgc acgctggatg
     3241 gtccttcgct gggatggtga aaccatgaaa aatggcagct tcagtggatt aagaggggtt
     3301 aatgtggcct gtaccatctg gttgcatagg tattcatgcg gttaaaattt atcaggcgcg
     3361 atcgcggcag tttttcgggt ggtttgttgc catttttacc tgtctgctgc cgtgatcgca
     3421 ccgaacgcgt tttggcggtg cgtacaatta agggattatg gtaaatccaa gaataacgaa
     3481 aaagcccccg gtgggggctt ttgagatatc aagcgtattg ggttaatttc caagttacgg
     3541 cttttccgtt atacggaggc attacattgc ctttggcaat tggtgctgta gttgaaggcg
     3601 ttcctaccac tttccaaaca ccgctttcag ggcatttttg tccggttgaa gctgttgtac
     3661 caattggggc tttagacata ttgattcctc ttgtatgttt tatccgtatg cttgtgtagc
     3721 aatacttatt tctggtttag aaatcagctt tcaagcgggc ttaaaggtcg ttttttcaca
     3781 gatgtgattg tggtcatatt tttaacaaag ttgtatatga ttttggcgtt gtaagttttg
     3841 gttttgttat aaaggactta tggaaaatca acttactgcc tgccctcgta gccatcgaga
     3901 taaacagccg aaatggtgtc agccagcagc ctggattttt tcggggtgag gtctttgctg
     3961 cggtaatcgg tacccgcaaa ttgtgttgtg cctcggagtg agtttaaaaa ttcctgactg
     4021 tgaaagtgct gaatgcgttg ctgcacatca ctctgtgatt ttcctgtcgc tttatcttta
     4081 gctcccatat cccagaactg ccgaaaaaaa ggcaccatca ttcgtgttgt gtcggccttc
     4141 tgggtggaaa agttaggatc aaaaccgtca tcagaagcat atacgtaatg ccgggtatgt
     4201 tgttccacgc tgccctttgg ggtacaggct gtgagagcta ctgttgctgt tatcatggcg
     4261 ataagacaca gtttgtttcc ggtacgtctc atgagtgtct ccctaccttc aagtgtttat
     4321 tctcggtgag ttattatccg aagactattt tatcatcagt ctctgacagc gttcagcgac
     4381 cgaacagcct ggaccagaat ccccgtttct tttccagttc cagtgcctgc ctgagctggg
     4441 ctatctcatt ctgcagctgt tcccgttctg cttcctggcg tttgcgatcc atatcctgtg
     4501 cctgtttgtc ctcaagcatc agcgtcaggc attgtttcag ctcattcagc tcccggagaa
     4561 tgcgttctgt cggctgatca tgtggatttt ctgcctgtcc cgcactgtgc ctttctggtg
     4621 tctcattctg ctttaattcg ccgtatgccc ggatcagttc actggtttca aactcccgtc
     4681 gtccgtcttg actggtacgg taactgacaa agcctgcgcg catatcacgg taaaactgtg
     4741 agcgcgatct gcctgtcatt ttcagtgcgt cacggagtgt gtgccatgcc ataaagtgac
     4801 agtgtcccat aggtgtctca tctcatggtt tcagtaaaac ataatgaggt ctgagaacgg
     4861 taatgtttgt gctggttttt gtggtatccg gcgggaatag cacgtggtgt gggtggctgt
     4921 ttttttgatc gttttcacaa aaacgaaaat tcccagggtt gacaggaaaa acgcagcggc
     4981 gtaattttaa actgtatata aaaccagtgg ttatgtgtac agtatttatt tttaacggtt
     5041 tgttttaaaa gtcaaagagg gatttattat gggtagtaat ggcgcagata atgcacataa
     5101 caatgctttt ggtggaggga aaaatccggg cattggtaat accagtggcg caggaagtaa
     5161 tggtagtgca tcaagtaacc gaggaaattc caatggatgg tcatggagta ataagcctca
     5221 taaaaatgat ggcttccaca gtgatggttc ttaccatatt acatttcatg gggacaataa
     5281 ttcaaagcct aaacctggag ggaatagtgg aaatcgaggt aataacggtg atggagcgag
     5341 tgctaaggtt ggagagataa caatcacacc tgacaactcg aaaccaggtc gttatatttc
     5401 gtcaaatcct gaatattcat tgttggcaaa attaattgat gcggaatcaa ttaaaggtac
     5461 agaggtatat acttttcaca ccagaaaagg tcagtatgtt aaggttactg ttccagatag
     5521 taatattgat aaaatgagag ttgattatgt gaactggaag ggaccgaaat ataacaataa
     5581 acttgtgaag aggtttgtga gccagttttt attatttagg aaggaagaaa aagaaaaaaa
     5641 tgaaaaagaa gccttgctaa aggctagtga acttgtgtct ggtatggggg ataagcttgg
     5701 cgagtatctt ggagtaaaat ataaaaatgt agctaaggaa gttgccaatg atattaaaaa
     5761 cttccatggt cgtaatattc gtagctataa tgaagcaatg gcttcactta ataaagtgtt
     5821 agcaaatcca aagatgaaag taaacaaatc tgataaggat gccattgtga atgcctggaa
     5881 acaggttaat gctaaggaca tggctaataa gattggtaat cttggcaagg catttaaggt
     5941 tgctgattta gctataaagg ttgagaaaat tagggaaaaa agcattgagg gatacaatac
     6001 tggcaactgg ggacctctcc tgttggaggt tgaatcatgg atcattggtg gcgttgttgc
     6061 tggagttgct attagtttat tcggggctgt gttgagtttt ctcccaatct ctggacttgc
     6121 agttactgcg ttgggggtaa taggaataat gacgattagt tacttgtcat ctttcataga
     6181 tgcaaatcga gtttcgaata taaataacat tatatctagt gttattcgat gatattagtg
     6241 tcaggggagt attcctcccc tggtaaatag cttatatgtt cctataagga accaaaagta
     6301 caaatatgtg aatatatata tcccaatata tagtgttata tagaaaaaag acaaaaacag
     6361 atcgtttttt gacattaata caaatgtttt tgatgacaag gttagttctg tgtcagttag
     6421 caagaaacag tatatcatgg atatataaag aatagtgaaa ggagttaata tctttagtgc
     6481 ccgcagaggt gacgactttg gttttaactc taattctttg tgtgtgaata tgaataagct
     6541 gattgcagct aaaggggctg tatgaacata gagattgaat gcttcgctta ggggcgggtt
     6601 gtttaatgaa tgaaatcctg gtagggatag gtatgcgatg attttttcga ggagtggtat
     6661 tggtgcatta ttataactga aaaatattaa tgcgaatggg gaaagtaaga gcagaagact
     6721 gaatgatatt ttttttgata tcttatttct gtcttttata tccatgttgc tttctttgga
     6781 tggttgaaaa ttatgtgtgg gaaaatttta t
//
