LOCUS       CP057881                4671 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB11-C23 plasmid pRHB11-C23_3, complete
            sequence.
ACCESSION   CP057881
VERSION     CP057881.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148075
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH10 T1-C23
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.7601629482882x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 12:30:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,959
            Genes (coding)                    :: 4,848
            CDSs (with protein)               :: 4,848
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 46 of 111
            Pseudo Genes (incomplete)         :: 55 of 111
            Pseudo Genes (internal stop)      :: 30 of 111
            Pseudo Genes (multiple problems)  :: 15 of 111
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB11-C23"
                     /isolation_source="Pooled cattle faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB11-C23_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            667..858
                     /locus_tag="HVW58_25350"
     CDS             667..858
                     /locus_tag="HVW58_25350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMN97314.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGETL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1844..1975)
                     /locus_tag="HVW58_25355"
     CDS             complement(1844..1975)
                     /locus_tag="HVW58_25355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QMN97315.1"
                     /translation="MRGLVFCALILILPGCQANYIRDVQGGTVAPSSSSAATGIEIR"
                     /name="colicin release lysis protein"
     gene            complement(1987..2466)
                     /locus_tag="HVW58_25360"
     CDS             complement(1987..2466)
                     /locus_tag="HVW58_25360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001485345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMN97316.1"
                     /translation="MIKRLLFLYVALLFLSEGAYASVTVFIAPSWERNTDSVNEIYKYI
                     DDINSNNHVIDQAYVEPIRKELGVYRDYIQKKLINFNGKGVCKLSITTGSTGVKDIEPP
                     DVVLLNSLAENSSLDCKKLYREIQSIVDNDPSLLFPEKIKSNGLLSIDMIIVMGR"
                     /name="hypothetical protein"
     gene            complement(2549..3307)
                     /locus_tag="HVW58_25365"
     CDS             complement(2549..3307)
                     /locus_tag="HVW58_25365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMN97317.1"
                     /translation="MSGIENSNQGPGWHDHNTPYGTIHSYDGDSHSGYGAHSGGNGGNR
                     HEGISDKTHISIPSTRNYNGYYMSMCVNNLKAHEGFKNTMYKDSEGNITVGIGHLLSTP
                     DMAASLPFTRTHTWHGHGDDMEKEVAASKKEITDAFNNFKKDSRQVPMNLHLSNDAVIG
                     LCISDVQTTEKGLRSLYSGYDGFSNSRKTALVDMGFNLGIPKLSRNFPNFNAAVNRGDW
                     ESAARESHRNLHQRGDQRNKDTADQLRDKH"
                     /name="hypothetical protein"
     gene            3561..3998
                     /locus_tag="HVW58_25370"
     CDS             3561..3998
                     /locus_tag="HVW58_25370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QMN97318.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTSQDGRR
                     EFETSELIRAYGELKRNETPERHTAGQAEKPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAEREQLQNEIAQLRQALEQERKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            4079..4507
                     /gene="exc2"
                     /locus_tag="HVW58_25375"
     CDS             4079..4507
                     /gene="exc2"
                     /locus_tag="HVW58_25375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMN97319.1"
                     /translation="MKRSGGTRYMYLSALITVSAALTACTPKTSLEQHTRHYVYASDDG
                     FDPNFYTKKADTTRMMIPFFQQFREMGVKDKAAGVSAETAQQRIKEFHSEEFLQSLRTT
                     TTFAGRKYTNSDMPSPEKMKLLADTISAIYLDGYEGRQ"
                     /name="exc2"
     Typing          1025..1572
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.374"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tctgtttggg cgcatacggt gcccatgagg cacattttcg gacattttca gttcgtgaat
       61 ccctggctca ttgctacata tccgggcaga cacccctggc agcgccccca ggggtgttat
      121 gtaaaaaaag gcgacgcgcc cctaggggcg cccctacatt aattaactgt tttttctaca
      181 gatagtcagt actgacccta agcagggtcg tcatagcgtc gccatagggt cgtcgacgac
      241 gtgcttttgt ataaatctga ataagaaaat acatttttaa aaccataaca acatgattta
      301 aaaagaaaat cagcaaaaag ttggttttgc gtggggtgtg gacatttcag acatgagaac
      361 agactcgcgt ttttccggag tatcagcgga atatctgatt aaaaaacact cgccgcagag
      421 cgcgatttta tgcaacagcg gctgtactgc acagggatta tttatcagtt gatatgctgt
      481 gccatgatat tgctattgcc gaaagggacg tcgttttacg gcgttttccc tatcctgcat
      541 cgcactggcc gcgttcgaat gtttgcgccc ctgactaacc gctcactcag ctttatcact
      601 ctcgcctgcg tcacagttat catgctccca tgaacagaaa ttcccttaca cggagacatc
      661 agaccagtga ccaaacagga aaaaaccgcc ctgaacatgg cccgcttcat cagaagccag
      721 acattaaccc tgctggagaa gctcaacgaa ctggacgcgg atgaacaggc agacatctgt
      781 gaatcgcttc acgaccacgc cgatgagctt taccgcagtt gcctcgcacg tttcggggat
      841 gacggtgaaa ccctctgaca tatgcagctc ccggagacgg tcacagcttg tctgtgagcg
      901 aatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gttttagcgg gtgtcggggc
      961 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaat catgcggcat
     1021 gtccggtgtg aaatgccgca cagatgcgta aggagaaaat gccagtatag gcgctcttcc
     1081 gcttcctcgc tcactgactc gctacgctcg gtcgttcggc tgcggcgagc ggtgtctact
     1141 cactcaaaag ctgtaataca gttatccaca gaatcagggg ataatgccgg aaagaacatg
     1201 tgagcaaaaa ggcaaaacca gggcaaaagg ccgcgccgct ggcgtttttc cataggctcc
     1261 gcccccctga cgagcatcac aaaaattgac gctcaagtca gaggtggcga aacccgacag
     1321 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1381 ccctgccact taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1441 atagctcacg ctgttggtat ctcggcttgg tgtaggtcgt tcgctccaag ctgggctgtg
     1501 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1561 ccaacccggt aagacacgac ttaacgccac tggcagcagc cactggtaga ggatgtacaa
     1621 gaaattgctg atacgtagtt cttgaagtga aggaccgact gcggctacac tgaaaggaca
     1681 gatttggtgg cagcggtctt gtacaccctg ttacctcggg ttagaaagcc tgagaaactt
     1741 ctaaccttcg aaagaaccca cgcctgagaa cgtgggtttt ttcgtttaca ggcagcagat
     1801 tacgcgtaga aaaaaaggat ctcaagaaga tcctttaatc tttttaccta atttcgattc
     1861 cagtagctgc tgagctagaa gatggcgcaa cagtccctcc ttgaacatcc ctgatataat
     1921 ttgcctgaca gccgggcaat attaaaatta gcgcacaaaa aaccaaacca cgcatagaat
     1981 tgtattttat ctccccataa caatgatcat atcaattgat aacaaaccat ttgactttat
     2041 tttctcagga aataaaagtg acggatcgtt atcaacaata ctttgtattt ctctatacag
     2101 ttttttacag tctaatgatg agttttccgc caatgagttt aacaaaacaa catctggagg
     2161 ctcaatatct ttaacacctg tacttcctgt tgttatcgat aatttacaaa ctcccttccc
     2221 attaaagtta attagttttt tctggatata atctcgatat acccctaact ccttcctaat
     2281 aggttctaca tatgcttgat cgatgacatg gttattacta tttatatcat caatatattt
     2341 atatatctca ttaactgaat ctgtattcct ttcccaactt ggagcaatga atactgttac
     2401 agatgcataa gcaccttctg ataagaacaa taaagcaaca tataagaata ataaccgctt
     2461 gatcatataa tccattccca ttataggttg atgtatttca tctttaatat aagatgaaat
     2521 acatctcata tctatatgtt atggtctatt aatgtttatc ccgcaactga tctgctgtat
     2581 ccttgtttct ctgatcgcct cgttgatgaa gatttcgatg gctttcacgt gctgcagact
     2641 cccaatcccc acggttcaca gcagcattaa agttcggaaa gtttctcgat agcttaggaa
     2701 ttcctaagtt aaatcccata tccactaagg cagtttttct agagttagaa aaaccatcat
     2761 aaccagaata taaagaacgc aagcctttct ctgtagtctg aacatcggaa atacataatc
     2821 caataacagc atcatttgat aaatgcaagt tcattggtac ttgtctagag tcttttttaa
     2881 agttattaaa agcatcggtt atttcttttt ttgatgctgc aacttctttt tccatatcat
     2941 caccatgacc atgccatgta tgagttcttg taaaagggag agatgccgcc atatctggcg
     3001 tagatagcaa atgaccaata ccaacagtaa tattaccttc gctatcctta tacatggtgt
     3061 ttttgaaccc ctcatgggct tttaaattat taacacacat gctcatatag tagccattat
     3121 agtttctggt agatggtata ctaatatgtg tcttatcaga aataccttca tggcgatttc
     3181 ccccattacc accagaatga gcaccataac cgctatggct gtccccatca taactatgaa
     3241 ttgttccata gggagtattg tgatcatgcc accccggacc ttgattagaa ttttcaatac
     3301 cactcatctt aatttcctct ttgacattta aaacaacgat caatacaact gtatatataa
     3361 ccactagttt tatatacagt ttaaaattac gccgctgcgt tttccctgtc aaccctgtgg
     3421 attttcattt ttgtgaaaac ggtcaaaaaa acagccactc acaccatgtg ccatccccgc
     3481 cagatgccac aaaaaccagc acaaacatta ccgttctccg gcctcagtat gttttactga
     3541 gaccatgaga tgagacacct atgggacact gtcactttat ggcatggcac acactccggg
     3601 acgcactgaa aatgacgggc agatcgcgtt cacagtttta ccgtgatatg cgcgcaggcc
     3661 ttgtcagtta ccgtacaagc caggacggac gacgggagtt tgaaactagt gaactgatcc
     3721 gggcatacgg cgaattaaag cggaatgaga caccagaaag gcacacagcg ggacaggcag
     3781 aaaaacctca tgatcagcag acagaacgca ttctccggga gctgaacgag ctgaaacaat
     3841 gcctgacgct gatgcttgag gataaacaag cccaggatac ggagcgccgg cgccaggaag
     3901 cagaacggga acaactacag aatgagatag cccagctcag gcaggcgctg gagcaggaaa
     3961 ggaaacgggg attctggtcc cggttgtttg gtcgctgaac gctgtcagag actgatgata
     4021 agatagtctt ctgataataa ctcaccgaaa ataaatacat taaggtaagg agactcgcat
     4081 gaaacgttct ggcggcacac gctatatgta tcttagcgcc ctgataactg tgtcagcagc
     4141 cctcacagcc tgtaccccaa aaaccagcct ggaacaacat acccggcatt atgtttatgc
     4201 ttcagatgat gggttcgatc ctaacttcta caccaagaaa gctgacacca cacggatgat
     4261 gattccgttc tttcagcagt tcagggagat gggggtgaaa gacaaagcag ccggagtatc
     4321 agcagaaacg gcacagcaac gcataaaaga attccactct gaagaattcc ttcagtcact
     4381 gagaaccaca accacctttg cgggcagaaa atacacgaac agtgatatgc catcaccgga
     4441 aaaaatgaag ctgctggctg acacgatttc tgcgatttat ctcgatggct acgagggaag
     4501 acagtaagtg gatttaccat aatcccttaa ttgtacgcac cattaaaacg cgttgagcgc
     4561 gattctgacg caggcacagc aaaaagaaat gtttaccccc aggaaaaccg gaaaaatgcg
     4621 tcaggatcgt tttcagggcg ttgcatgact atgcacgaaa ctgaatagcg a
//
