LOCUS       CP057858                6668 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB13-C01 plasmid pRHB13-C01_5, complete
            sequence.
ACCESSION   CP057858
VERSION     CP057858.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148089
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6668)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH03 T1-C01
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 94.6313732682813x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 11:57:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,903
            Genes (coding)                    :: 4,762
            CDSs (with protein)               :: 4,762
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 63 of 141
            Pseudo Genes (incomplete)         :: 70 of 141
            Pseudo Genes (internal stop)      :: 27 of 141
            Pseudo Genes (multiple problems)  :: 19 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB13-C01"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB13-C01_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(459..650)
                     /locus_tag="HVW72_25045"
     CDS             complement(459..650)
                     /locus_tag="HVW72_25045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMN42927.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            714..1037
                     /locus_tag="HVW72_25050"
     CDS             714..1037
                     /locus_tag="HVW72_25050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMN42928.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1195..1737
                     /locus_tag="HVW72_25055"
                     /pseudo=""
     CDS             1195..1737
                     /locus_tag="HVW72_25055"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1715..2233
                     /locus_tag="HVW72_25060"
     CDS             1715..2233
                     /locus_tag="HVW72_25060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbeB"
                     /protein_id="QMN42929.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="mobilization protein MbeB"
     gene            2240..2494
                     /gene="mbeD"
                     /locus_tag="HVW72_25065"
     CDS             2240..2494
                     /gene="mbeD"
                     /locus_tag="HVW72_25065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMN42930.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSEQTERLSQLYSENWR"
                     /name="mbeD"
     gene            complement(2812..3231)
                     /gene="exc2"
                     /locus_tag="HVW72_25070"
     CDS             complement(2812..3231)
                     /gene="exc2"
                     /locus_tag="HVW72_25070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMN42931.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(3312..3749)
                     /locus_tag="HVW72_25075"
     CDS             complement(3312..3749)
                     /locus_tag="HVW72_25075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QMN42932.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            4009..5577
                     /gene="cea"
                     /locus_tag="HVW72_25080"
     CDS             4009..5577
                     /gene="cea"
                     /locus_tag="HVW72_25080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="QMN42933.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(5574..5915)
                     /locus_tag="HVW72_25085"
     CDS             complement(5574..5915)
                     /locus_tag="HVW72_25085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="QMN42934.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEHIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin transporter"
     gene            5963..6100
                     /locus_tag="HVW72_25090"
     CDS             5963..6100
                     /locus_tag="HVW72_25090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QMN42935.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     Typing          287..374
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(4..273)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.75"
                     /coverage="99.0"
                     /evalue="3.21e-110"
     Typing          complement(6349..6668)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.885"
                     /coverage="99.0"
                     /evalue="7.819999999999999e-150"
     Typing          1028..2598
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.809"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgcctg ctacgcggcc
       61 ttcttcctgt ttctggtctt ttgctcacat gttctttccg gccttatccc ctgattctgt
      121 ggataactgt gttaccgttt ttgtgtgagt cagtaccgct cgccgcagtc gaacgaccga
      181 gcgtagcgag tcagtgagcg aggaagcgga aaagcgcctg gacgtgcatt ttctccttac
      241 gcatctgtgc ggcatttcac acccggcatg gcgtactttt catacaatcc gcactgatgc
      301 cgcatggtta agccagtata cactccgcta tcgctacgtg actgggtcag ggctgcgccc
      361 cgacacccgc taaaacctgc tgacgcgccc tgacgggctt gtcagctccc ggcatccgct
      421 cacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatcc
      481 ccgaaacgtg cgaggcagct gcggtaaagc tcatcggcgt ggtcgtgaag cgattcacaa
      541 atatcggcct gttcatctgc gtccagttcg ttgagcttct ccagcagcgt taatgtctgg
      601 cttctgataa agcgggccat gttaagggcg gttttttcct gtttagtcac tgatgcctcc
      661 gtgtaagggg gatttctgtt catggggtaa tgataccgat gaaacgcgag aggatgctca
      721 caatacgggt tactgatgat gaacatgccc ggttacttga acgctgtgag ggtaaacaac
      781 tggcggtatg gatgcggcgg gtctgcctgg gggagccggt tgcccgttcc ggaaaactgc
      841 cgacactggc accgccgtta ctgcgtcagc tggccgccat cggaaataac ctgaatcaga
      901 cagcccgtaa ggtgaacagc gggcagtggt cttccggtga ccgggttcag gtggtggccg
      961 cactgatggc catcggggat gagctgcgcc ggctgcgtct ggccgtcagg gaacaggggg
     1021 cgcgggatga tagttaaatt tcatgccagg ggaaaaggtg gtggcagtgg tccggttgat
     1081 tacctgctgg ggagggagcg taaccgcgaa ggcgcaacgg tgcttcaggg taatccggaa
     1141 gaagtccggg aactcatcga tgccacgcca tttgcgaaga aatacacgtc cggtgttctg
     1201 tcgttcgcgg agaaggagct gccgccggga ggacgtgaaa aagtgatggc gagctttgag
     1261 cgtgtactga tgcccggtct cgaaaaaaat cagtacagca tcctgtgggt ggagcaccag
     1321 gacaagggac ggcttgagct gaattttgtc attccgaaca tggagctaca gaccggaaaa
     1381 cgcctccagc cgtactacga ccgcgcagac aggcctagaa ttgatgcctg gcagacgctg
     1441 gtgaatcacc attacgggct gcatgacccg aacgccccgg agaaccgcag gacgctgaca
     1501 ctccctgata acctgcctga aacgaaacag gcgcttgctg agggcgtcac gcgaggtata
     1561 gatgcacttt accatgccgg agagataaaa ggccgtcagg atgtgattca ggcgctcact
     1621 gaggcggggc tggaagtggt cagggtgacg cgaagcagta tcagcattgc agatccgaac
     1681 ggcgggaaga atatcaggct gaaaggagca ttttatgagc aatcttttgc agacgggcgc
     1741 ggagttcgag aaaaagctga aagagagagc cgaatctaca gagaaaatgc tgaacaacga
     1801 gttcaggagg ctcggcgaat ctgtaagcga ggctgtgaca tcaaacgaga cgaaaatcag
     1861 agacgctata gccctgttca cagcctcgac agaggaatcg ctggaaaaac accgggaagg
     1921 ggtgaaagag gcgatgatgc agcacaggag ggacgtgtta aagctggcag ggaatacggg
     1981 catgatgtta ctggggatag tctttctcct gtttaccgcg agtggcggga cgctctggta
     2041 tcttggaggg aggatacagg cgaacctgga agaaatcagg aagcaggaag agacattgca
     2101 gaaactgaac gcgaagacat ggggcgtgga gtttgtgcag gacgggaaca ggaaattcct
     2161 tgtccttccg tacgggaaat cagcggaggt gattcccttt caggggaaag agtgggtaca
     2221 tctgaaggag tgacacagag tgacagagct ggaaacacat ttgctgaacg ccttagagca
     2281 gctgcaacag gactatatgc agcggctgag cgaatgggag agcgccttcg tggaattgca
     2341 gaagatgttt tcgcttacgc aacgggacaa cgcgatgctg aacgagcggg tcatgcagtt
     2401 gagtcagcag gtgcagcact tgagcgagca gacagaacgc ttgagcgagc agacagaacg
     2461 cttgagccag ttatacagcg agaactggag ataagagagg aacggctgat acaggagcgc
     2521 gaacatgtgt tatccctgga acgggagcgt cagccggaaa tacaggaacg cacgctggat
     2581 ggcccttcgc tgggatggtg aaaccatgaa aaatggcagc ttcagtggat taagtggggg
     2641 taatgtggcc tgtaccctct ggttgcatag gtattcatac ggttaaaatt tatcaggcgc
     2701 gatcgcggca gtttttcggg tggtttgttg ccatttttac ctgtctgctg ccgtgatcgc
     2761 gctgaacgcg ttttagcggt gcgtacaatt aagggattat ggtaaatcca cttactgtct
     2821 gccctcgtag ccatcgagat aaaccgcaga aatcgtgtca gccagcagcc tggatttttt
     2881 cggggtaagg tctttgctgc ggtaatcagt acccgcaaat tgagttgtgc cccggagtga
     2941 gtttaaaaat tcctggctgt gaaactgctg aatgcgttgt tgcacatcac tccgtgattt
     3001 tcctgtcgct ttatctttag ctcccatatc ccagaactgc cgaaaaaaag gcaccatcat
     3061 tcgtgttgtg tcggcttttt gggtggaaaa gttgggatca aaaccgtcat cagaagcata
     3121 tacgtaatgc cgggtatgtt gttccacgct gccctttggg gtacaggctg tgagagctac
     3181 tgttgctgtt atcatggcga taagacaaag tttgtttccg gtacgtctca tgagtgtctc
     3241 cctaccttaa agtatttatt ctcggtgagt tattatccga agactatttt atcatcagtc
     3301 tctgacagcg ttcagcgacc gaacaacctg gaccagaatc cccgtttctt ttccagttcc
     3361 agtgcctgcc tgagctgggc tatctcattt tgtagctgtt cccgttctgc ttcctggcgt
     3421 ctgcgatcca tatcctgtgc ctgtttatcc tcaagcatca gcgtcaggca ttgtttcagc
     3481 tcattcagct cccggagaat gcgttctgtc tgctgatcat gtggattttc tgcatgtccc
     3541 tcactgtgcc tttctggtgt ctcattctgc tttaattcgc cgtatgcccg gatcagttca
     3601 ctggtttcaa actcccgtcg tccgtccctg ccggtacggt aactgacaag gcctgcgcgc
     3661 atatcacggt aaaactgtga acgcgatctg cctgtcattt ttagtgcgtc ccggagtgtg
     3721 tgccatgcca taaagtgaca gtgtcccata gatgtctcat ctcatagttt cagtaaaaca
     3781 taatgaggtc tgagaacggt aatgtttgtg ctggtttttg tggcatcggg cgagaatagc
     3841 gcgtggtgtg aaagactgtt tttttgatcg ttttcacaaa aatggaagtc cacagtcttg
     3901 acagggaaaa tgcagcggcg tagcttttat gctgtatata aaaccagtgg ttatatgtac
     3961 agtatttatt tttaacttat tgttttaaaa gtcaaagagg attttataat ggaaaccgcg
     4021 gtagcgtact ataaagatgg tgttccttat gatgataagg gacaggtaat tattactctt
     4081 ttgaatggta ctcctgacgg gagtggctct ggcggcggag gtggaaaagg aggcagtaaa
     4141 agtgaaagtt ctgcagctat tcatgcaact gctaaatggt ctactgctca attaaagaaa
     4201 acacaggcag agcaggctgc ccgggcaaaa gctgcagcgg aagcacaggc gaaagcaaag
     4261 gcaaacaggg atgcgctgac tcagcgcctg aaggatatcg tgaatgaggc tcttcgtcac
     4321 aatgcctcac gtacgccttc agcaacagag cttgctcatg ctaataatgc agctatgcag
     4381 gcggaagcag agcgtttgcg ccttgcgaaa gcagaagaaa aagcccgtaa agaagcggaa
     4441 gcagcagaaa aggcttttca ggaagcagaa caacgacgta aagagattga acgggagaag
     4501 gctgaaacag aacgccagtt gaaactggct gaagctgaag agaaacgact ggctgcattg
     4561 agtgaagaag ctaaagctgt tgagatcgcc caaaaaaaac tttctgctgc acaatctgaa
     4621 gtggtgaaaa tggatggaga gattaagact ctcaattctc gtttaagctc cagtatccat
     4681 gcccgtgatg cagaaatgaa aacgctcgct ggaaaacgaa atgaactggc tcaggcatcc
     4741 gctaaatata aagaactgga tgagctggtc aaaaaactat caccaagagc caatgatccg
     4801 cttcagaacc gtcctttttt tgaagcaacc agacgacggg ttggggccgg taagattaga
     4861 gaagaaaaac aaaaacaggt aacagcatca gaaacacgta ttaaccggat aaatgctgat
     4921 ataactcaga tccagaaggc tatttctcag gtcagtaata atcgtaatgc cggtatcgct
     4981 cgtgttcatg aagctgaaga aaatttgaaa aaagcacaga ataatctcct taattcacag
     5041 attaaggatg ctgttgatgc aacagttagc ttttatcaaa cgctgactga aaaatatggt
     5101 gaaaaatatt cgaaaatggc acaggaactt gctgataagt ctaaaggtaa gaaaatcggc
     5161 aatgtgaatg aagctctcgc tgcttttgaa aaatacaagg atgttttaaa taagaaattc
     5221 agcaaagccg atcgtgatgc tatttttaat gcgttggcat cggtgaagta tgatgactgg
     5281 gctaaacatt tagatcagtt tgccaagtac ttgaagatta cggggcatgt ttcttttgga
     5341 tatgatgtgg tatctgatat cctaaaaatt aaggatacag gtgactggaa gccactattt
     5401 cttacattag agaagaaagc tgcagatgca ggggtgagtt atgttgttgc tttacttttt
     5461 agcttgcttg ctggaactac attaggtatt tggggtattg ctattgttac aggaattcta
     5521 tgctcctata ttgataagaa taaacttaat actataaatg aggtgttagg gatttaatta
     5581 ttctttattg atataaaaag tcctagcaat ccaaatggga ttgctaggac caaacaaagt
     5641 agattatata gcataaatag gtttaatttt gctacggggg cgttatttag gttttttctt
     5701 ctttcgaaaa aatctttctt tatgaagtta aaagctatgt gttcaatagc atattttgaa
     5761 tatggacata gaatagtgct tatcactatt gcatatagca tcttatctga cacaaggaaa
     5821 taatacccct cgctgttttt tgttataagg tatatatata taagtgtgca gtacaggcca
     5881 aataaaatat tttttatgta gtatcttaag ctcataaatt aaacctcgcc atatattctt
     5941 ttcattttat aaggatcgag ttatgaggaa aagatttttt gtgggaatat tcgcgataaa
     6001 cctccttgtt ggatgtcagg ctaactatat acgtgatgtt cagggaggga ccatcgcacc
     6061 atcctcctct tctaaactga cggggatcgc ggttcagtag aaaagatcaa aggatcttct
     6121 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6181 acggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6241 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagtcggg ccactacttc
     6301 aagaactctg tagcaccgtt tgtgccatca tcgctctgct aatccggtta ccagtggctg
     6361 ctgccagtgg cgttaaggcg tgccttaccg ggttggactc aagacgatag ttaccggata
     6421 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6481 cctacaccga actgagatac caacagcgtg agctatgaga aagcgccacg cttcccgaag
     6541 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6601 agcttccagg gggaaacgcc tggtgtcttt atagtcctgt cgggtttcgc cacctctgac
     6661 ttgagcgt
//
