LOCUS       CP057853                6230 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB13-C02 plasmid pRHB13-C02_5, complete
            sequence.
ACCESSION   CP057853
VERSION     CP057853.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148090
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH03 T1-C02
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 67.2974232189073x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 12:01:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,019
            CDSs (total)                      :: 4,897
            Genes (coding)                    :: 4,752
            CDSs (with protein)               :: 4,752
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 63 of 145
            Pseudo Genes (incomplete)         :: 74 of 145
            Pseudo Genes (internal stop)      :: 27 of 145
            Pseudo Genes (multiple problems)  :: 19 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB13-C02"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB13-C02_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            468..1487
                     /locus_tag="HVW73_25065"
     CDS             468..1487
                     /locus_tag="HVW73_25065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QMN38174.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKC"
                     /name="replication initiation protein"
     gene            complement(1748..2146)
                     /locus_tag="HVW73_25070"
     CDS             complement(1748..2146)
                     /locus_tag="HVW73_25070"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMN38175.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQENERKQAVQNRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQELKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            2323..3249
                     /locus_tag="HVW73_25075"
     CDS             2323..3249
                     /locus_tag="HVW73_25075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QMN38176.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIEGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFETAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREYGFD
                     MRDGVSRQERTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            3252..3785
                     /locus_tag="HVW73_25080"
     CDS             3252..3785
                     /locus_tag="HVW73_25080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMN38177.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            4006..5733
                     /locus_tag="HVW73_25085"
     CDS             4006..5733
                     /locus_tag="HVW73_25085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="QMN38178.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENNP
                     WGGGSGSGVHWGGGSGNGNGGGNGNSGGGSNSSVAAVAFGFPALATPGAGTLGIAVSGE
                     ALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVTNVQV
                     STLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASFPGVP
                     SLTVSTVKGLPVSTTLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKPIYVSV
                     TDVLTPAQVKQRQDEENRLQQEWNDTHPVEVAERNYEQARAELDQANKDVARNQERQAK
                     AVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQRAQTDVN
                     NKKAAFDAAAKDKSDADAALGAALERRKQKENKEKDSKDKLDKESKRNKPGKATGKGKP
                     VGDKWLDDAGKDSGAPIPDRIADKLRDKEFKSFDDFRKKFWEEVSKDPELSKQFKDSNK
                     TNIQKGKAPFARKKDQVGGRERFELHHDKPISQDGGVYDMDNIRVTTPKRHIDIHRGK"
                     /name="colicin"
     gene            5736..5996
                     /locus_tag="HVW73_25090"
     CDS             5736..5996
                     /locus_tag="HVW73_25090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="QMN38179.1"
                     /translation="MELKHSISDYTEAEFLEFVKKICRAEGATEEDDNKLVREFERLTE
                     HPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKPGFKQG"
                     /name="bacteriocin immunity protein"
     gene            6058..6201
                     /locus_tag="HVW73_25095"
     CDS             6058..6201
                     /locus_tag="HVW73_25095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QMN38180.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     BGC             1..6230
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          469..1243
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          2324..3246
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.403"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgtcaggct gattctgatg cgcttttttt tgaaatgtca caaaaattcc atgcgggaga
       61 tgagagctaa aatccccgtg cagaactttc cacccagggc gagaaaactt gtcattttga
      121 cctgttcgcc cttcggaaca gtcgaaatcg atcgcgcatc gctttcgtgc atagttatgc
      181 agccctctaa aaacgattct ggcgcgtttt tctggtttgg cctggtgttt tcttgtcttt
      241 ttgcgttttt tgcgtcagaa agcgactgag ggcgttttaa ggggtgcgta caacgggagt
      301 tatggtaaat ggatcggttt ttcgggaagg atcgacagga tttgccgttg ggtgtagtgt
      361 aagcggctga aaaacaaacg cccctgaaat catgtccaga tccggcaaga ttcaacatga
      421 aatacagagg gcgtgtaggt cgcataaccc ggagagaatc gtaacacatg agcgccgcgc
      481 ttcaatactt cgacgaaaat ttaccccatc gtccctatca cacggatgat ctcgctttcg
      541 gccttcgcat ctccggcaaa gggcgtgcgc ttcttgcgcg gtacatccag cagaaccagc
      601 ctcatgcgca gttctggctg gtttttgacg ttgaccgcga gggggctgcg attgactgga
      661 gcgaccggaa cgcccccgcg ccgaacatca ccgttaaaaa tcctgtgaac ggacacgctc
      721 acctgctcta cgcactcaac atcgccgtga gaaccgcgcc tgatgcctcg gttaaggcgc
      781 tgaaatatgc cgcagcggtt gaacgttcgc tgtgtgagaa actttgtgct gatgtgaact
      841 acagcggcct gatttgcaaa aatccgttcc acctggaatg gcaggtgatg gagtggcgcg
      901 aggacgccta taccctcgat gaactggcag attatctcga tttgagcgcc tcagagcgtc
      961 gtagcatcga taaacattac gggatggggc gaaactgcca cctgttcgaa atgacgcgca
     1021 aatgggctta cagggcgatt cgtcagggct ggccagcgtt ctcacagtgg cttgaagcgg
     1081 tgatccagcg tgtcgaaatg tacaatgcat cgcttcccgt tccgctttca ctggctgaat
     1141 gtcgggctat tggcaagagc attgcgaaat acacgcacag gaacttcacg ccggaaactt
     1201 tcgcacagta tgtggctgat acgcacacgc cagaaattca ggcagcacgc ggtcgcaagg
     1261 gcgggaaggc caattcgtca gaaaatcagt cggacaaagg gaaaaaaagc gccgctgttc
     1321 ggtggactgc taacgacgac aaacgtcgta gggcgcttga tatgtatatc ttgggagcgt
     1381 caacggaaga tattgcagtg gcggttggtg ttagcagccg gactattcga cgctggatgg
     1441 ataattctgg agaatggcta accaagaaac aaataattaa atgttagtta acaaagaagc
     1501 aacactaccc cccgtttttt gttaatggga tgttaaattc gcggacatga acaagcctat
     1561 atcagataac agcgcctttc ggcgtctttt tgagcagcgg gctttccggc ggttttctcc
     1621 tctcagccca gcaatggtgc ggcttaccgc atgattgagg tggtcgtgct cgccgcagtc
     1681 tcatgaccga gcgtagcgag cgaatgagcg aggaagcacc agggaaccgg aacacggatg
     1741 gaggcactta cgcgtctggg tttggggatg tctccagatc gtacttaatt tccttaagtt
     1801 cctgtaatat cctgtctttt ttatagcata gctcgcctgt ttttgtgtct accagtcctg
     1861 cttcgatcag tagcccagcc gcgagaaaga gtcggtgatt gcgttctcgt cgttgttttt
     1921 ttccctcgat tcgattttgg accgcctgtt ttcgttcgtt ctcctgtgcc tgtaagtcgt
     1981 ttttttccgc tgttgccagt acgtggagga gtttgcgttc ctgttctgtg agtcctgctt
     2041 cgttgtcgag caggtcaata atctgtctct gtcgtgcggt ctgttctccg agcttcctga
     2101 tgaatccgat ccgtttcttc agccattcga tgttgctgat gcgcatgttc gtggtgctcc
     2161 ctggtgtttt tttgcggtgt gttgtggtcg tgcagtatat cgcttgcggt cggcgggtgc
     2221 aggaggtatc ttccgtgcta attcgtaaaa ttagaaggaa gcgcacttat atgtttctct
     2281 ccttcgtcga gaaacagtgc gctcctgcgg agggctgaaa ccgtggcaac ctatcacctc
     2341 agcgttaaat ttggtggcaa ggggcaggct gcaaaccacg ccgattacat cgagcgcaaa
     2401 gagaaatacc gcgaccgtca ggatttggaa tattccgcac acggaaacat gccggaatgg
     2461 gcgcgagata atccctcaca tttctggcag gcggctgacc agtttgaacg tgcaaacggt
     2521 tcgacttatc gggaactgga gatagcgctg ccccgtgaac tgacaccgga acagcgcctt
     2581 gaactggtgc aggattttgt acgtcaggaa gctggcgaac ggcatgcatg gagttttgcc
     2641 attcacaatc cgaaagcgag cattgaggga ggcgaacagc ctcatgcgca tatcatgatg
     2701 agtcagcgtg tgaatgatgg cattgaacgt acaccggaac agtatttccg gcgctataac
     2761 gccaggtatc ccgagcgggg aggtgcgaaa aaggattcag gaagcctgac tcccactcag
     2821 caaaaagagc agttgcggga attgcgaaaa cgctgggagg tgaagcacaa cgaacatatg
     2881 agaaagcatg gctttgaaag gggcttcatt gactgccgga cgctgaaaga acagggcatt
     2941 gaacgacgtc ctgaagtgca tctgggtttt gaaactgccg gacggcttga tgatgcccag
     3001 cgtcatgaca tcatgcaaaa acggtctgaa ccggactatt cccgccatct tgaacaggcg
     3061 cgaacggtcg ggcatgacat cagggaatgg ctggacactc cggttaagca acatgaactg
     3121 gtcagaaggc tgcaacatga tgttgcgcgg gagtatggct ttgacatgag agacggagtg
     3181 agcagacagg agcgaactga acctgaacgg gttaaatcac cgtcacgtgg attttctatg
     3241 ggattctgaa catggccagc acagaagaaa aattactcac gctcctgtta tcggtggagc
     3301 atctgcaaca taccgcaatg gagcaacaga aagctgttgc tgattctctg gctgtggcaa
     3361 gggaaacact tcgcggatat cgggatattt ctgacggtgt ggcaaaacag gtcagggatg
     3421 aagtcagaaa tgaggtgcag aagctggacg tggcaggact gataggtgag cgcatcagcc
     3481 aggattttca gcggctggaa gagtcagtcg caaacatgaa ggcaaacgcg gatgccctga
     3541 acagggatat tgcgacaatt cacagcaata tcgtgcagga atacaacacc cttaaagggt
     3601 atagctggaa atggctgggt ggggtgtcag tggcttttct ggttatgttc tgtgtgctcg
     3661 gatggggaat gaaaacaata ctcgacagga actacaacgc cacagcggcg gtgtatcaga
     3721 aagttgtgac actggaagat aaactggtgg agaaacctgt cacgggtaag agccggaaaa
     3781 actgacgcgc ttcgcttgtc caacgcctca cgacccgcga aaggatttcg cgttccgttc
     3841 accgttttca tgagtgattc ctccgaactg ccaaaaaata tccccccctg acttgacaga
     3901 aaaaataatg acgagtactt tttgatctgt acataaaacc agtggtttta tgtacagtat
     3961 taactatgta atcaattgtt ttaacgctta aaagagggaa tttttatgag cggtggagat
     4021 ggacgcgacc acaacagtgg cgcacataac acaggtggta acattaatgg cggccctacg
     4081 gggcttggtg gaaatggtgg ggcttctgac ggttcaggat ggagttcgga aaataatcca
     4141 tggggtggcg gttccggtag tggtgttcac tggggaggtg gctccggcaa tggtaatggc
     4201 ggggggaatg gtaattccgg tggtggcagc aattcatccg tagcagcagt ggcatttggt
     4261 tttcctgctt tggcaactcc tggtgccgga acgctgggta tcgccgtctc tggtgaggct
     4321 ttatctgcgg caatagctga tatctttgca gccctgaaag gtccgtttaa attcagcgca
     4381 tggggtattg cgctttacgg cattctgcca tctgaaatag caaaagatga cccgaatatg
     4441 atgtcaaaga ttgtgacgtc attaccggca gaaacagtga cgaatgttca ggtcagtaca
     4501 cttccgctgg accaggcaac tgtcagcgtg acgaaacgtg tgacagatgt tgtgaaggac
     4561 acacgacagc atattgccgt agttgcaggt gtgcctatga gtgtgcctgt tgtgaatgcc
     4621 aaaccaacac gtactccagg tgtatttcac gcatcatttc ctggtgtccc ttctctgaca
     4681 gtcagcactg tcaaaggcct tccggtgtca acaacccttc cccgtggtat tacggaggat
     4741 aaaggccgga ctgccgttcc tgcaggattt acctttggtg gtggttcaca tgaagcggtg
     4801 atccgttttc cgaaagaaag tgggcagaag ccgatttatg tatcagtgac agatgttctt
     4861 acccctgcac aggtaaaaca acgtcaggat gaggaaaatc gcctccagca ggaatggaat
     4921 gacacacatc ctgtagaagt ggctgaacgc aattatgaac aggcccgcgc agaactggat
     4981 caggctaata aagatgttgc cagaaaccag gaacgacagg ctaaagctgt tcaggtttat
     5041 aattctcgta aaagtgaact tgatgctgcg aataaaactc ttgctgatgc aaaggctgaa
     5101 ataaaacaat tcgagcgatt tgcccgagaa ccaatggctg ctggtcaccg aatgtggcaa
     5161 atggcagggc ttaaggccca gcgggcacag acggatgtaa ataataagaa ggctgcattt
     5221 gatgctgctg caaaagataa gtctgatgct gatgctgcgt taggtgctgc gctggaacgc
     5281 cgtaagcaga aggaaaataa agaaaaggac tctaaggata aattagataa ggagagtaaa
     5341 cggaataagc cagggaaggc gacgggtaaa ggaaaaccag ttggtgataa atggcttgat
     5401 gatgcgggta aagattcagg agcgccaatt cctgatcgca ttgctgataa gttgcgtgat
     5461 aaagaattta aaagttttga cgatttccgg aagaaattct gggaagaagt gtcaaaagat
     5521 cccgagctta gtaagcaatt taaagacagt aataagacga acattcaaaa gggaaaagca
     5581 ccttttgcaa ggaagaaaga ccaagtaggt ggtagggaac gctttgaatt acatcatgat
     5641 aaaccaatca gtcaggatgg tggtgtctat gatatggata atatcagagt gaccacacct
     5701 aagcgacata ttgatattca tagaggtaag taaaaatgga actgaaacat agtattagtg
     5761 attataccga ggctgaattt ctggagtttg taaaaaaaat atgtagagct gaaggtgcta
     5821 ctgaagagga tgacaataaa ttagtgagag agtttgagcg attaactgag cacccagatg
     5881 gttcagatct gatttattat cctcgcgatg acagggaaga tagtcctgaa gggattgtta
     5941 aagaaattaa agaatggcga gctgctaacg gtaagccagg atttaaacag ggctaaaata
     6001 tgaatgccgg ttgtttatgg atgaatggct ggcattcttt cacaacaagg agtcgttatg
     6061 aaaaaaataa cagggattat tttattgctt cttgcagtca ttattctggc tgcatgtcag
     6121 gcaaactata tccgggatgt tcagggcggg actgtatccc cgtcatcaac agctgaagtg
     6181 accggattag caacgcagta acccgaaatc ctctttgaca aaaacaaagc
//
