LOCUS       CP057847                6668 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB13-C03 plasmid pRHB13-C03_5, complete
            sequence.
ACCESSION   CP057847
VERSION     CP057847.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148091
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6668)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH03 T1-C03
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 89.3139305636394x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 12:01:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,028
            CDSs (total)                      :: 4,906
            Genes (coding)                    :: 4,764
            CDSs (with protein)               :: 4,764
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 63 of 142
            Pseudo Genes (incomplete)         :: 71 of 142
            Pseudo Genes (internal stop)      :: 27 of 142
            Pseudo Genes (multiple problems)  :: 19 of 142
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB13-C03"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB13-C03_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            13..336
                     /locus_tag="HVW74_25060"
     CDS             13..336
                     /locus_tag="HVW74_25060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMN33413.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            494..1036
                     /locus_tag="HVW74_25065"
                     /pseudo=""
     CDS             494..1036
                     /locus_tag="HVW74_25065"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1014..1532
                     /locus_tag="HVW74_25070"
     CDS             1014..1532
                     /locus_tag="HVW74_25070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbeB"
                     /protein_id="QMN33414.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="mobilization protein MbeB"
     gene            1539..1793
                     /gene="mbeD"
                     /locus_tag="HVW74_25075"
     CDS             1539..1793
                     /gene="mbeD"
                     /locus_tag="HVW74_25075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMN33415.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSEQTERLSQLYSENWR"
                     /name="mbeD"
     gene            complement(2111..2530)
                     /gene="exc2"
                     /locus_tag="HVW74_25080"
     CDS             complement(2111..2530)
                     /gene="exc2"
                     /locus_tag="HVW74_25080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMN33416.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(2611..3048)
                     /locus_tag="HVW74_25085"
     CDS             complement(2611..3048)
                     /locus_tag="HVW74_25085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QMN33417.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            3308..4876
                     /gene="cea"
                     /locus_tag="HVW74_25090"
     CDS             3308..4876
                     /gene="cea"
                     /locus_tag="HVW74_25090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="QMN33418.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(4873..5214)
                     /locus_tag="HVW74_25095"
     CDS             complement(4873..5214)
                     /locus_tag="HVW74_25095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="QMN33419.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEHIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin transporter"
     gene            5262..5399
                     /locus_tag="HVW74_25100"
     CDS             5262..5399
                     /locus_tag="HVW74_25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QMN33420.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(6426..6617)
                     /locus_tag="HVW74_25105"
     CDS             complement(6426..6617)
                     /locus_tag="HVW74_25105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMN33421.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          6254..6341
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(5685..6240)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.52"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          327..1897
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.809"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaacgcgaga ggatgctcac aatacgggtt actgatgatg aacatgcccg gttacttgaa
       61 cgctgtgagg gtaaacaact ggcggtatgg atgcggcggg tctgcctggg ggagccggtt
      121 gcccgttccg gaaaactgcc gacactggca ccgccgttac tgcgtcagct ggccgccatc
      181 ggaaataacc tgaatcagac agcccgtaag gtgaacagcg ggcagtggtc ttccggtgac
      241 cgggttcagg tggtggccgc actgatggcc atcggggatg agctgcgccg gctgcgtctg
      301 gccgtcaggg aacagggggc gcgggatgat agttaaattt catgccaggg gaaaaggtgg
      361 tggcagtggt ccggttgatt acctgctggg gagggagcgt aaccgcgaag gcgcaacggt
      421 gcttcagggt aatccggaag aagtccggga actcatcgat gccacgccat ttgcgaagaa
      481 atacacgtcc ggtgttctgt cgttcgcgga gaaggagctg ccgccgggag gacgtgaaaa
      541 agtgatggcg agctttgagc gtgtactgat gcccggtctc gaaaaaaatc agtacagcat
      601 cctgtgggtg gagcaccagg acaagggacg gcttgagctg aattttgtca ttccgaacat
      661 ggagctacag accggaaaac gcctccagcc gtactacgac cgcgcagaca ggcctagaat
      721 tgatgcctgg cagacgctgg tgaatcacca ttacgggctg catgacccga acgccccgga
      781 gaaccgcagg acgctgacac tccctgataa cctgcctgaa acgaaacagg cgcttgctga
      841 gggcgtcacg cgaggtatag atgcacttta ccatgccgga gagataaaag gccgtcagga
      901 tgtgattcag gcgctcactg aggcggggct ggaagtggtc agggtgacgc gaagcagtat
      961 cagcattgca gatccgaacg gcgggaagaa tatcaggctg aaaggagcat tttatgagca
     1021 atcttttgca gacgggcgcg gagttcgaga aaaagctgaa agagagagcc gaatctacag
     1081 agaaaatgct gaacaacgag ttcaggaggc tcggcgaatc tgtaagcgag gctgtgacat
     1141 caaacgagac gaaaatcaga gacgctatag ccctgttcac agcctcgaca gaggaatcgc
     1201 tggaaaaaca ccgggaaggg gtgaaagagg cgatgatgca gcacaggagg gacgtgttaa
     1261 agctggcagg gaatacgggc atgatgttac tggggatagt ctttctcctg tttaccgcga
     1321 gtggcgggac gctctggtat cttggaggga ggatacaggc gaacctggaa gaaatcagga
     1381 agcaggaaga gacattgcag aaactgaacg cgaagacatg gggcgtggag tttgtgcagg
     1441 acgggaacag gaaattcctt gtccttccgt acgggaaatc agcggaggtg attccctttc
     1501 aggggaaaga gtgggtacat ctgaaggagt gacacagagt gacagagctg gaaacacatt
     1561 tgctgaacgc cttagagcag ctgcaacagg actatatgca gcggctgagc gaatgggaga
     1621 gcgccttcgt ggaattgcag aagatgtttt cgcttacgca acgggacaac gcgatgctga
     1681 acgagcgggt catgcagttg agtcagcagg tgcagcactt gagcgagcag acagaacgct
     1741 tgagcgagca gacagaacgc ttgagccagt tatacagcga gaactggaga taagagagga
     1801 acggctgata caggagcgcg aacatgtgtt atccctggaa cgggagcgtc agccggaaat
     1861 acaggaacgc acgctggatg gcccttcgct gggatggtga aaccatgaaa aatggcagct
     1921 tcagtggatt aagtgggggt aatgtggcct gtaccctctg gttgcatagg tattcatacg
     1981 gttaaaattt atcaggcgcg atcgcggcag tttttcgggt ggtttgttgc catttttacc
     2041 tgtctgctgc cgtgatcgcg ctgaacgcgt tttagcggtg cgtacaatta agggattatg
     2101 gtaaatccac ttactgtctg ccctcgtagc catcgagata aaccgcagaa atcgtgtcag
     2161 ccagcagcct ggattttttc ggggtaaggt ctttgctgcg gtaatcagta cccgcaaatt
     2221 gagttgtgcc ccggagtgag tttaaaaatt cctggctgtg aaactgctga atgcgttgtt
     2281 gcacatcact ccgtgatttt cctgtcgctt tatctttagc tcccatatcc cagaactgcc
     2341 gaaaaaaagg caccatcatt cgtgttgtgt cggctttttg ggtggaaaag ttgggatcaa
     2401 aaccgtcatc agaagcatat acgtaatgcc gggtatgttg ttccacgctg ccctttgggg
     2461 tacaggctgt gagagctact gttgctgtta tcatggcgat aagacaaagt ttgtttccgg
     2521 tacgtctcat gagtgtctcc ctaccttaaa gtatttattc tcggtgagtt attatccgaa
     2581 gactatttta tcatcagtct ctgacagcgt tcagcgaccg aacaacctgg accagaatcc
     2641 ccgtttcttt tccagttcca gtgcctgcct gagctgggct atctcatttt gtagctgttc
     2701 ccgttctgct tcctggcgtc tgcgatccat atcctgtgcc tgtttatcct caagcatcag
     2761 cgtcaggcat tgtttcagct cattcagctc ccggagaatg cgttctgtct gctgatcatg
     2821 tggattttct gcatgtccct cactgtgcct ttctggtgtc tcattctgct ttaattcgcc
     2881 gtatgcccgg atcagttcac tggtttcaaa ctcccgtcgt ccgtccctgc cggtacggta
     2941 actgacaagg cctgcgcgca tatcacggta aaactgtgaa cgcgatctgc ctgtcatttt
     3001 tagtgcgtcc cggagtgtgt gccatgccat aaagtgacag tgtcccatag atgtctcatc
     3061 tcatagtttc agtaaaacat aatgaggtct gagaacggta atgtttgtgc tggtttttgt
     3121 ggcatcgggc gagaatagcg cgtggtgtga aagactgttt ttttgatcgt tttcacaaaa
     3181 atggaagtcc acagtcttga cagggaaaat gcagcggcgt agcttttatg ctgtatataa
     3241 aaccagtggt tatatgtaca gtatttattt ttaacttatt gttttaaaag tcaaagagga
     3301 ttttataatg gaaaccgcgg tagcgtacta taaagatggt gttccttatg atgataaggg
     3361 acaggtaatt attactcttt tgaatggtac tcctgacggg agtggctctg gcggcggagg
     3421 tggaaaagga ggcagtaaaa gtgaaagttc tgcagctatt catgcaactg ctaaatggtc
     3481 tactgctcaa ttaaagaaaa cacaggcaga gcaggctgcc cgggcaaaag ctgcagcgga
     3541 agcacaggcg aaagcaaagg caaacaggga tgcgctgact cagcgcctga aggatatcgt
     3601 gaatgaggct cttcgtcaca atgcctcacg tacgccttca gcaacagagc ttgctcatgc
     3661 taataatgca gctatgcagg cggaagcaga gcgtttgcgc cttgcgaaag cagaagaaaa
     3721 agcccgtaaa gaagcggaag cagcagaaaa ggcttttcag gaagcagaac aacgacgtaa
     3781 agagattgaa cgggagaagg ctgaaacaga acgccagttg aaactggctg aagctgaaga
     3841 gaaacgactg gctgcattga gtgaagaagc taaagctgtt gagatcgccc aaaaaaaact
     3901 ttctgctgca caatctgaag tggtgaaaat ggatggagag attaagactc tcaattctcg
     3961 tttaagctcc agtatccatg cccgtgatgc agaaatgaaa acgctcgctg gaaaacgaaa
     4021 tgaactggct caggcatccg ctaaatataa agaactggat gagctggtca aaaaactatc
     4081 accaagagcc aatgatccgc ttcagaaccg tccttttttt gaagcaacca gacgacgggt
     4141 tggggccggt aagattagag aagaaaaaca aaaacaggta acagcatcag aaacacgtat
     4201 taaccggata aatgctgata taactcagat ccagaaggct atttctcagg tcagtaataa
     4261 tcgtaatgcc ggtatcgctc gtgttcatga agctgaagaa aatttgaaaa aagcacagaa
     4321 taatctcctt aattcacaga ttaaggatgc tgttgatgca acagttagct tttatcaaac
     4381 gctgactgaa aaatatggtg aaaaatattc gaaaatggca caggaacttg ctgataagtc
     4441 taaaggtaag aaaatcggca atgtgaatga agctctcgct gcttttgaaa aatacaagga
     4501 tgttttaaat aagaaattca gcaaagccga tcgtgatgct atttttaatg cgttggcatc
     4561 ggtgaagtat gatgactggg ctaaacattt agatcagttt gccaagtact tgaagattac
     4621 ggggcatgtt tcttttggat atgatgtggt atctgatatc ctaaaaatta aggatacagg
     4681 tgactggaag ccactatttc ttacattaga gaagaaagct gcagatgcag gggtgagtta
     4741 tgttgttgct ttacttttta gcttgcttgc tggaactaca ttaggtattt ggggtattgc
     4801 tattgttaca ggaattctat gctcctatat tgataagaat aaacttaata ctataaatga
     4861 ggtgttaggg atttaattat tctttattga tataaaaagt cctagcaatc caaatgggat
     4921 tgctaggacc aaacaaagta gattatatag cataaatagg tttaattttg ctacgggggc
     4981 gttatttagg ttttttcttc tttcgaaaaa atctttcttt atgaagttaa aagctatgtg
     5041 ttcaatagca tattttgaat atggacatag aatagtgctt atcactattg catatagcat
     5101 cttatctgac acaaggaaat aatacccctc gctgtttttt gttataaggt atatatatat
     5161 aagtgtgcag tacaggccaa ataaaatatt ttttatgtag tatcttaagc tcataaatta
     5221 aacctcgcca tatattcttt tcattttata aggatcgagt tatgaggaaa agattttttg
     5281 tgggaatatt cgcgataaac ctccttgttg gatgtcaggc taactatata cgtgatgttc
     5341 agggagggac catcgcacca tcctcctctt ctaaactgac ggggatcgcg gttcagtaga
     5401 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     5461 aaaaaaacca ccgctaccaa cggtggtttg tttgccggat caagagctac caactctttt
     5521 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     5581 gtagtcgggc cactacttca agaactctgt agcaccgttt gtgccatcat cgctctgcta
     5641 atccggttac cagtggctgc tgccagtggc gttaaggcgt gccttaccgg gttggactca
     5701 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5761 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc aacagcgtga gctatgagaa
     5821 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5881 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtgtcttta tagtcctgtc
     5941 gggtttcgcc acctctgact tgagcgtcta tttttgtgat gctcgtcagg ggggcggagc
     6001 ctatggaaaa acgcctgcta cgcggccttc ttcctgtttc tggtcttttg ctcacatgtt
     6061 ctttccggcc ttatcccctg attctgtgga taactgtgtt accgtttttg tgtgagtcag
     6121 taccgctcgc cgcagtcgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaaaa
     6181 gcgcctggac gtgcattttc tccttacgca tctgtgcggc atttcacacc cggcatggcg
     6241 tacttttcat acaatccgca ctgatgccgc atggttaagc cagtatacac tccgctatcg
     6301 ctacgtgact gggtcagggc tgcgccccga cacccgctaa aacctgctga cgcgccctga
     6361 cgggcttgtc agctcccggc atccgctcac agacaagctg tgaccgtctc cgggagctgc
     6421 atgtgtcaga ggttttcacc gtcatccccg aaacgtgcga ggcagctgcg gtaaagctca
     6481 tcggcgtggt cgtgaagcga ttcacaaata tcggcctgtt catctgcgtc cagttcgttg
     6541 agcttctcca gcagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
     6601 ttttcctgtt tagtcactga tgcctccgtg taagggggat ttctgttcat ggggtaatga
     6661 taccgatg
//
