LOCUS       CP057836                6878 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB13-C13 plasmid pRHB13-C13_4, complete
            sequence.
ACCESSION   CP057836
VERSION     CP057836.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148097
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6878)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6878)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH03 T1-C13
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 103.298558814832x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 12:06:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,946
            CDSs (total)                      :: 4,821
            Genes (coding)                    :: 4,713
            CDSs (with protein)               :: 4,713
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 44 of 108
            Pseudo Genes (incomplete)         :: 51 of 108
            Pseudo Genes (internal stop)      :: 26 of 108
            Pseudo Genes (multiple problems)  :: 11 of 108
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB13-C13"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB13-C13_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(232..363)
                     /gene="ctl"
                     /locus_tag="HVW80_24650"
     CDS             complement(232..363)
                     /gene="ctl"
                     /locus_tag="HVW80_24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 10 lysis protein"
                     /protein_id="QMN15531.1"
                     /translation="MKITFLIITILSLSGCQANYISDVQGGTVAPSSSSELTGIAVQ"
                     /name="ctl"
     gene            388..678
                     /gene="cti"
                     /locus_tag="HVW80_24655"
     CDS             388..678
                     /gene="cti"
                     /locus_tag="HVW80_24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 10 immunity protein"
                     /protein_id="QMN15532.1"
                     /translation="MTVKYYLHNLLESLIPWLFYLLLNYKTPPFSLFIFIASIHVLLYP
                     YSKLTIFSFIQNTTKMKKEPWYSYNLFYFLYLAMAIPVGLPSFIYYSLKRN"
                     /name="cti"
     gene            complement(675..2147)
                     /gene="cta"
                     /locus_tag="HVW80_24660"
     CDS             complement(675..2147)
                     /gene="cta"
                     /locus_tag="HVW80_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin 10"
                     /protein_id="QMN15533.1"
                     /translation="MDKVTDNSPDVESTESTEGSFPTVGVDTGDTITATLATGTENVGG
                     GGGAFGGASESSAAIHATAKWSTAQLKKHQAEQAARAAAAEAALAKAKSQRDALTQRLK
                     DIVNDALRANAARSPSVTDLAHANNMAMQAEAERLRLAKAEQKAREEAEAAEKALREAE
                     RQRDEIARQQAETAHLLAMAEAAEAEKNRQDSLDEEHRAVEVAEKKLAEAKAELAKAES
                     DVQSKQAIVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLERQLQDKKNEYSSVTNALN
                     SAVSIRDAKKTEVQNAEIKLKEAKDALEKSQVKDSVDTMVGFYQYITEQYGEKYSRIAQ
                     DLAEKAKGSKFNSVDEALAAFEKYKNVLDKKFSKVDRDDIFNALESITYDEWAKHLEKI
                     SRALKVTGYLSFGHDVWDGTLKGLKTGDWKPLFVTLEKSAVDFGVAKIVALMFSFIVGA
                     PLGFWGIAIITGIVSSYIGDDELNKLNELLGI"
                     /name="cta"
     gene            2392..2682
                     /locus_tag="HVW80_24665"
     CDS             2392..2682
                     /locus_tag="HVW80_24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMN15534.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            complement(2674..2952)
                     /locus_tag="HVW80_24670"
     CDS             complement(2674..2952)
                     /locus_tag="HVW80_24670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMN15535.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            3074..3496
                     /gene="exc2"
                     /locus_tag="HVW80_24675"
     CDS             3074..3496
                     /gene="exc2"
                     /locus_tag="HVW80_24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMN15536.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGISAETAQQRVKEFHSEKFFHSLRSTTA
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(3980..4213)
                     /gene="mbeD"
                     /locus_tag="HVW80_24680"
     CDS             complement(3980..4213)
                     /gene="mbeD"
                     /locus_tag="HVW80_24680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMN15537.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="mbeD"
     gene            complement(4220..4780)
                     /locus_tag="HVW80_24685"
     CDS             complement(4220..4780)
                     /locus_tag="HVW80_24685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QMN15538.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAALKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(4752..5495)
                     /locus_tag="HVW80_24690"
                     /pseudo=""
     CDS             complement(4752..5495)
                     /locus_tag="HVW80_24690"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5458..5781)
                     /locus_tag="HVW80_24695"
     CDS             complement(5458..5781)
                     /locus_tag="HVW80_24695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMN15539.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGHWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5848..6039
                     /locus_tag="HVW80_24700"
     CDS             5848..6039
                     /locus_tag="HVW80_24700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMN15540.1"
                     /translation="MTKQEKTALNMARFIRSQTLILLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6228..6464)
                     /locus_tag="HVW80_24705"
     CDS             complement(6228..6464)
                     /locus_tag="HVW80_24705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMN15541.1"
                     /translation="MEKAPVPRPFPGSSFFAHMFFPGLSPDSVDNGVTAFEKGESARRS
                     RTTERSESVSEEAEEHRKLHFLLTHLCGISHSA"
                     /name="hypothetical protein"
     Typing          complement(6126..6213)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="2.44e-33"
     Typing          complement(6671..6858)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.296"
                     /coverage="100.0"
                     /evalue="2.18e-82"
     Typing          complement(3874..5468)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.12"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgagatttat aagttagtaa cagagttctt gaagtatcag cctgattttg actacactag
       61 aaggacaagt tttgggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
      121 gtagatcttg atccggcaaa caaaccaccg ttggtagcgg tggttttttt gtttgcaagc
      181 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctactgaac
      241 cgcgatccct gtcagttcag aagaggaaga tggagcgacc gtccctcctt gtacatcact
      301 aatataattt gcttgacatc ccgacaagga taatattgta attatcaaaa acgttatttt
      361 cattctaccc ctataacaac ataaataatg accgtaaaat actacctaca caatttacta
      421 gagtcactta tcccatggct attctatttg ttattaaact ataagacgcc cccattttca
      481 cttttcatat tcatcgcatc tatacatgta ttactatacc catattcaaa actaactata
      541 ttttctttta tacaaaacac gacaaaaatg aaaaaagaac cttggtactc atacaacctt
      601 ttttattttc tttacctagc aatggcaatc ccagtaggat tgccaagttt catatattac
      661 tctctaaaga gaaattaaat acctagcaat tcattaagct tgttcaactc atcatcccct
      721 atgtaagaag aaacaatacc tgtgataatt gcaattcccc agaagccaag aggcgcacca
      781 acaataaaac taaacattaa tgccacaatt tttgccacgc cgaaatctac cgcgctcttc
      841 tccagagtga caaataaagg cttccagtct cctgttttta atccctttag ggtaccatcc
      901 catacatcat gcccgaaaga caaatatcca gtaaccttaa gagctctaga gatcttttct
      961 agatgcttgg cccactcatc ataagtaata gactctaaag cattaaaaat atcatcccta
     1021 tcaaccttac tgaatttctt atccagtaca tttttatact tttcaaatgc agcaagtgct
     1081 tcatcaacac tattaaattt actacccttc gccttttcag ctaaatcctg agctattctg
     1141 gaatattttt ccccatattg ttcggttata tattgataaa acccaaccat agtatcaaca
     1201 gagtctttta cctgactctt ctcaagagca tccttagctt cttttaattt tatctcagca
     1261 ttctgaactt ctgttttttt agcatctcta atgctaacag cagaattaag agcattcgtc
     1321 actgacgaat attcattctt cttatcctgc aattgtctct ccagtttttt ctgaacatca
     1381 cgccatccag gaaatccggt aaccttcaca tcaacacttt tttgagcgtt ttcaagctcc
     1441 cctgcaactc tggaaacaat cgcttgctta ctctgtacat cgctttcggc cttcgccagt
     1501 tcagctttag cctcagccag cttcttctct gccacttcca cagcccgatg ctcttcatca
     1561 agagaatcct gtcgattttt ctcagcctct gctgcctccg ccattgctaa caaatgcgcg
     1621 gtttcagcct gttggcgggc aatctcatca cgttggcgtt ctgcttcccg gagcgctttt
     1681 tctgctgctt cagcttcttc acgggctttt tgctctgcct tcgcaaggcg caaacgctca
     1741 gcctctgcct gcattgccat attattggca tgagcaaggt cagttactga tggactacgg
     1801 gcggcattag cacgtaaagc gtcattaaca atatccttga gacgttgagt cagggcatca
     1861 cgctgagatt tcgcttttgc caatgctgcc tcagccgcag cagcacgggc agcctgttca
     1921 gcctgatgtt ttttcaactg cgcggtagac catttagcgg ttgcatgtat cgcagcagaa
     1981 ctttcactgg ccccaccaaa tgctccaccg cctccaccaa cattttcagt tccagttgca
     2041 agcgtcgctg taatcgtatc gccagtatca accccaacag ttgggaatga cccctcagta
     2101 gattctgtgc tctccacatc tggagaatta tcagtgactt tatccataaa gaaattcctc
     2161 tttgataatt aaaacaataa attaaaaaca atatactgta catataacca ctggttttat
     2221 gtacagtaaa aacctactac tcagcattgt ccatgtcaag agcatggatt ttcatttttg
     2281 caataaggat cacactatgg ggaggcaggc attgagaacg tcgaaacaga acaccggagc
     2341 aaatcaggat gagatataaa actgttggat catgaaaaaa cggagaacga tgtgagcaaa
     2401 tcaccccgcc ataaactgaa caaaacagac aaacgacttc tcgacaccct tgttgctgcc
     2461 ggatatgagc atgacaaagc ccgtgacctc atccagaaac aggtttacac gctgacactg
     2521 gcagatcagc gtcatgtggt cagtgaaatc agtaatggtg tgaatcccac ccaggcttac
     2581 tcggcggtat accaggcaag acgcattcgc ctcgcccgta aatatctgaa cggtaaaaag
     2641 gttatggaag aaaccgggga aaatacgccc ccatcagcgt aaggatttct tttgccgctc
     2701 cagagactcc agttttttac gcaaatcctc tcttttttgg gcatctctgg tgccagccag
     2761 ctctgctctc aactcatcga tctgaagttg tatcttcagt ctgttactga acattttctg
     2821 tctggcatta acatccgcaa caatgccgtt ttttgtcttc tcggcctttt gttgaaaaac
     2881 attgctgtcc gcatgactgg caaccgaagc agaaagaaca ctaaaaagca ggactggcac
     2941 acattttttc acgggattat tcctgactca ttgaccatca aatcacattg ggagtaaacc
     3001 gacgtatgat aagagatact cttcggatat ataactccct gagtatcaag attaaaaacg
     3061 caaggagatg tttatgagat ctgccgctgc caggctgctt ctgatacctc tgataacagc
     3121 aacaatagct cttacaggat gcacaccaaa gaccagcctg gaacgacata cccggcatta
     3181 tgtttatgct tcagatgatg gatttgatcc taacttctac acccagaaag cagacaccat
     3241 acgtatgatg ctcccgttct ttcagcagtt ccgggatatg gggatgaaag acaaagcagc
     3301 cggaatatca gcagaaacgg cacagcaacg tgtaaaagaa ttccactcag aaaaattttt
     3361 tcactcactc cggagcacaa cagcctttgc tggcagaaaa tacacaaaca gcgatatgcc
     3421 ttcgccgaaa aaaatgaaac taatggcaga caccatttct gcggtttatc tcgatggata
     3481 cgagggcaga cagtaaggga tttaccataa tcccttaatt gtacgcaccg ctgaaatgcg
     3541 ttcagcgcga tcacggctgc tgacaggtaa aaatggcaac aaaccacccg aaaagctgcc
     3601 gcgatcgcac ctgataaatt ttaaccgtat gcatagctat tcagccatgt gaataacgct
     3661 ggttttgcct gcgtaaacct catgacactg ttttttttcc atcttttcag ttgatgacat
     3721 acgcagacat cgcgggatga ggctgaggaa tgagcgcgat ctggcaaaga ggcaaaacac
     3781 agcaacaaaa acgacacgcc agaatcgcgc ccggatgcat ttttaacgcg ttccggtacc
     3841 atctggcaac ctcccggaac aactcaccgt cacatcccca ttgacgggcc acgccatacc
     3901 cgtacttccc gttcctgctc ttcatgccag gaccgcgcac gctcccgttc caggcgtgcc
     3961 agcctttcct gttcatccct tatctgctgt tcgtgataaa taaccgactc aagcggtcca
     4021 cctgcccggc taatctctgc acctgctgac tcaagtcgtc gcactgttcc ctcagttgcc
     4081 cgttctcctg tcgtgtcagc tcgaacatat gctgcaaatc cgtgaaggcg ctctcccagt
     4141 ctttcagccg ctgcgtatag tcctgctgca attgctctaa ggcgttcagt aagtgcattt
     4201 ccagctctgt catactcact tactccctga ccagtcttac tgcgttcttc ttctccaccg
     4261 tccagttgtt ttcccccttc accccggacg gcaataccag aaatttcccg ttcctgccct
     4321 cgtgatacgt cacaccccat gttttttccc ggagtttcgc cagcgtctct tcctggtccc
     4381 tgatagccag gatgttcgcc gcaatccggc tttcctgcca ctgaatcagc ccccatgacg
     4441 ccagaaaaat cccgcccgtg acgcaaagcg ccgtcagcga cgggtacagt atccgccctt
     4501 tgatcagctt ccagagcagc tcttcctgcc gccgggccag ttcgttctct gtggcgctga
     4561 actgcgcgtt cacggcactg ttcagcgtct ccagttgttc tttcagcgct gctgtgtgtg
     4621 cgctgatagc gtctctgatt ttctgcccgt ttaagttcag ttccctgtct acagacgctt
     4681 cgagcttcct gaactcgctg ttcagcatgt tctctgtaga gacggcacgc tctttcagtt
     4741 tcttctcgaa gtctgtcccc atttgtaaaa gattgctcat acagcgcccc tttcagcctg
     4801 agattacgcc cgccctccgg gtcggcgata ctgatactgc tcctggttgt cctcaccacc
     4861 tcaaaacctg cctctgtaag cgcctcagtg acatcctgac gcgtttttag cgctccggca
     4921 tgataaagag cctccagccc cctcgtaatc gcttctgcgg cctcctgttt cgctttcggc
     4981 agattattcg gggtgacaag tgtccgcctg ttctccggtg cgttcgggtc gtgcagcccg
     5041 taatgatgat tcaccagtgt ctgccaggca ttgattcgcg gacggtccgc acggtcgtaa
     5101 tagggctgga gccgttttcc gctcaccagc tccatattcg ggatgacaaa attcagctca
     5161 agacgcccct tgtcctggtg ctccacccac aggatgctgt actgattttt ttcaagaccg
     5221 ggcatcagta cccgctcaaa gctctccatc accctttcac gctctcccgg tggcagtgtc
     5281 tgctctgcaa aagacagaac ccccgaggtg tattttttcg caaacggcgt ggcatcgatg
     5341 agttcccgca cctcttcggg atcaccccgc agaattctcg ccccttcccg gttacgctcc
     5401 cggcccagca ggtaatcaac cggaccactg ccaccgcctt ttcccctggc atgaaactta
     5461 actatcatcc cgttctccct gtttacggac ctcatccctc agctcactca gttcacgtcc
     5521 gatggccatc agtgcagcca ccacatgaac ccggtcatgc cccgaccagt gtccgctgtt
     5581 tatcttccgg gctatctgat tcaggttatt gccgaccgaa gcgaactggc gcaacagcgg
     5641 cggtgccagt gtcggaagac ctgacgtttt cgatggcggt gcacccaggc agaccttacg
     5701 catccatgac gcaagttgtt ttccctcaca acgtgccagc agccgcgcat gttcctcatc
     5761 cgtgacccgt atcgtgagca tcctttcgcg tttcaccggt atcattaaaa acctccgaca
     5821 gactccccac acatggagaa cagaactgtg actaaacagg aaaaaacagc ccttaacatg
     5881 gcccgcttta tcagaagcca gacgctgatc ctgctggaaa aactgaatga actggacgcc
     5941 gacgaccagg cggacatctg cgaagccctt cacgaccacg ccgacgagct ttaccgcagc
     6001 tgcctcgcac gcttcgggga tgacggtgaa aacctctgac acatgcagct cccggagacg
     6061 gtcacagctt gcctgtgagc ggatgccggg agcagacaag cccgtcaggg cacgtcagcg
     6121 ggttttagcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     6181 actggcttat accatgcaga atcagtgcgg attgtatgaa aagtgcatca tgccgagtgt
     6241 gaaatgccgc acagatgcgt aaggagaaaa tgcagcttcc ggtgctcttc cgcttcctcg
     6301 ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggactcccc tttctcaaag
     6361 gcggtaaccc cgttatccac agaatccggg gataacccgg gaaagaacat gtgcgcaaaa
     6421 aacgaagacc ccgggaaagg ccgcggcaca ggcgcttttt ccataggctc cgcccccctg
     6481 acggacttca caaaacagac actttttcgg tgcatctgtg ggagccttac ggctcaacca
     6541 tgaaatccgc cagcggtggc gaaacccgac gtggcttaaa gatcatcacc gtttccggtg
     6601 aatcgctccc tcctgcgccc ccacgttccc gccatatcga ttaccggata ccttttccgc
     6661 ctttctccct tgcgggacac gtggcgcttt ctcatagctc acgtcttctg gtatctcggg
     6721 gcgacttagg cagttcgctc cgggccgggc tgtatgcagg aaccccccgt tcagcccgac
     6781 tgctgcgcct tctccggtaa ctgttctctt gggtccaacc cggaaaggac acgacttaac
     6841 gccactggca gcagccattg gtaaccaggt tagcagag
//
