LOCUS       CP057828                7205 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB14-C07 plasmid pRHB14-C07_3, complete
            sequence.
ACCESSION   CP057828
VERSION     CP057828.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148107
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7205)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 7205)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH04 T1-C07
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 93.3603055529705x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 12:34:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,951
            CDSs (total)                      :: 4,829
            Genes (coding)                    :: 4,706
            CDSs (with protein)               :: 4,706
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 39 of 123
            Pseudo Genes (incomplete)         :: 75 of 123
            Pseudo Genes (internal stop)      :: 24 of 123
            Pseudo Genes (multiple problems)  :: 15 of 123
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB14-C07"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB14-C07_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(7005..7205,1..228))
                     /gene="exc2"
                     /locus_tag="HVW90_24710"
     CDS             complement(join(7005..7205,1..228))
                     /gene="exc2"
                     /locus_tag="HVW90_24710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMN02386.1"
                     /translation="MKRSGGTRYMYLSALITVAAALTACTPKTSLEQHTRHYVYASDDG
                     FDPNFYTKKADTTRMMIPFFQQFREMGVKDKAAGVSAETAQQRIKEFHSEEFLQSLRTT
                     TTFAGRKYTNSDMPSPEKMKLLADTISAIYLDGYEGRQ"
                     /name="exc2"
     gene            complement(638..928)
                     /locus_tag="HVW90_24715"
     CDS             complement(638..928)
                     /locus_tag="HVW90_24715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMN02379.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            1169..2668
                     /gene="csa"
                     /locus_tag="HVW90_24720"
     CDS             1169..2668
                     /gene="csa"
                     /locus_tag="HVW90_24720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /protein_id="QMN02380.1"
                     /translation="MAKELSGYGPTAGESVGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGGEHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPGAGGNSDVQG
                     GYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEK
                     AKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDNLPPNVRKFDVDVEQF
                     HYLVVLDDYGNVLSVTRTGVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEEPQRQ
                     NQAKKAAYDVFYSFSMNRDRIQSDILNKSAEVISDIGNKVGDYLGDAYKSLAREIADDV
                     KNFQGKTIRSYDDAMASLNKVLSNPGFKLNRADSDALANAWRSIDAQDMANKLGNFSKA
                     FKFADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASAVALGVFSATLGAYAL
                     SLGAPAIAVGIVGILLAAVVGALIDDKFADALNNEIIKPAH"
                     /name="csa"
     gene            complement(2696..3235)
                     /gene="csi"
                     /locus_tag="HVW90_24725"
     CDS             complement(2696..3235)
                     /gene="csi"
                     /locus_tag="HVW90_24725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 immunity protein"
                     /protein_id="QMN02381.1"
                     /translation="MMIDEHSIDIDNRKANNLLYLFMVIGVIPLLCILAVYYTNPDNLF
                     LHTIATSTENIPSITSAYNPLMTKVMDIYCKTAPFLALILFILTFKTRKPIKKINRNAV
                     LRSCLLSPFVCAVFLYLLCFRNLELTTAGRPARLMANNDATLLLFYIGLYLIIFFISYF
                     TLFTPVTTFKLLKERQ"
                     /name="csi"
     gene            3265..3420
                     /gene="csl"
                     /locus_tag="HVW90_24730"
     CDS             3265..3420
                     /gene="csl"
                     /locus_tag="HVW90_24730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="QMN02382.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GGSGSL"
                     /name="csl"
     gene            complement(4418..4609)
                     /locus_tag="HVW90_24735"
     CDS             complement(4418..4609)
                     /locus_tag="HVW90_24735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMN02383.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4673..4996
                     /locus_tag="HVW90_24740"
     CDS             4673..4996
                     /locus_tag="HVW90_24740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMN02387.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4938..5696
                     /locus_tag="HVW90_24745"
                     /pseudo=""
     CDS             4938..5696
                     /locus_tag="HVW90_24745"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5674..6204
                     /locus_tag="HVW90_24750"
     CDS             5674..6204
                     /locus_tag="HVW90_24750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QMN02384.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGMIQANLEEIRKQNENLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETNWTM
                     ENGKQNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            6211..6444
                     /gene="mbeD"
                     /locus_tag="HVW90_24755"
     CDS             6211..6444
                     /gene="mbeD"
                     /locus_tag="HVW90_24755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMN02385.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="mbeD"
     Typing          4246..4333
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          complement(3678..4221)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.796"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4987..6548
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.04"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttgtctttc acccccatct cccggaactg ctgaaagaac ggaatcatca tccgtgtggt
       61 gtcagctttc ttggtgtaga agttaggatc aaacccatca tctgaagcat aaacataatg
      121 ccgggtatgt tgttccaggc tggtttttgg ggtacaggct gtgagggctg ctgccacagt
      181 tatcagggcg ctaagataca tatagcgtgt gccgccagaa cgtttcatgc gagtctcctt
      241 accttaaagt atttattttc ggtgagttat tatctgatgg ctatcttatc ggggttcccg
      301 gccggaaacc tggatttttc tgcgacaagt ttttacggga gtgtcacaca aacgatcgat
      361 tgagtgactg tcactgaaac aacgatttcg acaccaaata tcgtcacata acccgtcacc
      421 tattcaacgt catgcaaatc tgtcgattac tagctttggt gacggaattt tctgggtttc
      481 cggcttagaa cccctgtatc ggaaatttcg gcgtttccgg cgtacaaccc caacttcaga
      541 tcgatgagtt gagagcagaa ctggctggca ccagagatgc ccaaaaagag aggatttgcg
      601 taaaaaactg gagtctctgg aacggcaaaa gaaatcctta cgctgatggg ggcgtatttt
      661 ccccagtttc ttccataacc tttttaccgt tcagatattt acgggcgagg cgaatgcgtc
      721 ttgcctggta taccgccgag taagcttggg taggattcac accgttactg atttcactga
      781 ccacatgacg ctgatcagcc agtgtcagcg tgtaaacctg tttctggatg aggtcacggg
      841 ctttgtcatg ctcatatccg gcagcaacca gggtgtcgag aagtcgcttg tctgttttgt
      901 tcagtttatg gcggggagat ttgctcacat cgttctccgt tttttcatga tccaacagtt
      961 ttatatctca tcctgatttg ctccggtgtt ctgtttcgac gttctcaatg tctgcctccc
     1021 catagtgtga tccttattgc aaaaatgaaa atccatgctc ttgacatgga taatgctgag
     1081 tagtaggttt tcactgtaca taaaaccagt ggttgtatgt acagtatatt gtttttaatt
     1141 tattgtttta attatcaaag aggaatttat ggctaaagaa ctaagtggat atggcccaac
     1201 tgctggtgag tctgtggggg gaacaggggc gaatctgaat cagcaaggtg gaaataataa
     1261 tagtaatagt ggggttcact ggggtggtgg ttcaggctct ggtaatggag gtggtgaaca
     1321 tggaagtcag acggggtggg gatggagtaa aaccaataat ccggatgtgc cgccttatgt
     1381 tgatgataat gggcaggtgc gcattaccat cacaaatggt ctggtaaaaa cgcctgtata
     1441 tggagttcct ggggctggcg ggaatagtga tgttcagggg ggctatattc ctgaaaatcc
     1501 taatgatgaa gttgctcgta agtgggataa aaacaactta ccgcgagaga ttgatgtgag
     1561 tatcgatggt tttaaatatc gtgtaacgct taatgataat ggtagagcta tcggtattct
     1621 tcgaacaggt gtccgtcctt acgtagggag tgaaaaagct aaggcgggta ttatggaaaa
     1681 aattaatcat aaaacacctg aggagatata tgaggcgtta ggttttaata aagatgaatc
     1741 tcagcggcag gaaaaagcaa aacaacaggc tgaagatgcg tgggataatt tgcctcctaa
     1801 tgttagaaaa tttgatgttg atgttgaaca gttccactat ttagtggtgc ttgatgatta
     1861 tggtaatgtt ttatctgtaa ctagaacagg tgttcgtcct tatgtaggga gtgaaaaagc
     1921 taaggctggt attatggata aagtcgacca taagaccccg gaagagatat atgaggcatt
     1981 aggatttaat aatgaagagc ctcaacgaca gaatcaagct aaaaaagctg cttatgatgt
     2041 gttttattct ttcagtatga accgtgaccg gattcagagt gatatactta ataaatccgc
     2101 tgaagttatc tctgatatag gtaataaggt cggtgattat cttggagatg cttataagtc
     2161 gttagcaaga gaaattgctg atgatgtcaa aaatttccag gggaaaacca tccgtagcta
     2221 cgatgatgca atggcgtcat taaataaagt tttgtctaat cctgggttta aacttaacag
     2281 ggccgacagt gatgcactgg cgaacgcctg gcgaagtatt gatgctcagg atatggcaaa
     2341 taaactgggt aatttcagca aggctttcaa atttgccgat gtggtgatga aggttgagaa
     2401 ggtccgggag aaaagcatcg agggctatga aaccgggaac tggggacctc tgatgctgga
     2461 ggtggagtcc tgggtgctca gtggcatcgc ttctgctgtg gccctgggtg ttttttcagc
     2521 cacattaggg gcatatgcct tatctcttgg ggctcctgct attgctgtcg gtatcgtagg
     2581 tattctactc gcagcagttg taggtgcatt aattgatgac aagtttgcag atgcactaaa
     2641 taacgaaatc attaaacccg ctcattaata aataccgggt aaaaggaagg cagggttact
     2701 gcctttcttt taataattta aacgtggtaa ctggtgtgaa tagcgtgaaa tagcttataa
     2761 aaaaaatgat taagtacagg ccaatataaa atagcaatag tgttgcatca ttgttggcca
     2821 ttagcctggc tggccttcct gctgtcgtta actcaaggtt tcggaagcag agtaaataca
     2881 aaaaaacagc acagacaaat ggacttaata agcaacttct aagtacagca tttcggttta
     2941 ttttttttat tggttttctc gttttaaatg tcaggataaa taatatcaaa gcaaggaacg
     3001 gcgctgtttt acaataaata tccataacct ttgtcattaa tgggttgtag gcggaggtta
     3061 ttgatgggat gttttcagta ctggtagcta ttgtgtgtaa gaacagattg tctggatttg
     3121 tatagtaaac ggctagtata cagagtaaag gtataactcc gattaccata aataaataca
     3181 ataagttatt ggcttttcta ttgtctatat ctattgaatg ttcatcaatc atcattgacc
     3241 tataggatta taataaaggt atatatgaaa aaaattatta tttgtgccat ttcactagga
     3301 ataatgctgc ttgcagcatg ccaagtcaac aatgtcagag atactggagg tggggctgtt
     3361 tcaccatctt ccactgttac cggagtgagt atgggaagtg gtggtagtgg tagcctgtaa
     3421 aggatcttct tgagatcctt tacagatcgt cgtagtctct tgccctgtaa acgaaaaaac
     3481 cgcctgggga ggcggtttag tcgaaggtta agtcagttgg ggactgctta accgggtagc
     3541 tggcttaagt ggagcacaga taccaaatgc tgtcctttca gtgtagccgc tataaggctt
     3601 caacttcaag agttctgcgt atctaaatcc acttttcagt taccaatggc tgctgccagt
     3661 ggcgttttgt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3721 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3781 aagccgagat accaacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3841 gcggacaggt atccggtaag tggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3901 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3961 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgcagc ggcgctgcct
     4021 tttgttgccc tttgcctttt tactcacatg ttctttccgg ccttatcccc tgattctgtg
     4081 gataaccgta tttccgcctt tgagtgagcc ggcaccgctt gccgcagccg aacgaccgag
     4141 cgtagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtatgt tctccttacg
     4201 catctgtgcg gcatttcaca ctcggcatgg cgtgcttttc atacaatccg cactgatgcc
     4261 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcagg gctgcgcccc
     4321 gacacccgct aaaacctgct gacgcgccct gacgggcttg tctgctcccg gcatccgctc
     4381 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatccc
     4441 cgaaacgtgc gaggcagctg cggtaaagct catcggcgtg gtcgtgaagc gattcacaga
     4501 tgtctgcctg ttcatccgcg tccagttcgt tgagcttttc cagcagcgtt aatgtctggc
     4561 ttctgataaa gcgcgccatg ttaagggctg ttttttcctg tttagtcact gatgcctccg
     4621 tgtaaggggg atttctgttc atggggtaat gataccgatg aaacgcgaga ggatgctcac
     4681 gatacgggtt actgatgatg aacatgccag gttacttgaa cgctgtgagg gtaaacaact
     4741 ggcggtatgg atgcggcggg tctgcctggg ggagccggtt gcccgttccg ggaaactgcc
     4801 gacactggca ccgccgttac tgcgtcagct ggccgccatc ggaaataacc tgaatcagac
     4861 agcccgtaag gtgaacagcg ggcagtggtc ttccggtgac cgggttcagg tggtggccgc
     4921 actgatggcc atcggggatg agctgcgccg gctgcgtctg gccgtcaggg aacagggggc
     4981 gcgggatgat agttaaattt catgccaggg gaaaaggtgg tggcagtggt ccggttgatt
     5041 acctgctggg gagggagcgt aaccgcgaag gcgcaacggt gcttcagggt aatccggaag
     5101 aagtccggga actcatcgat gccacgccat ttgcgaagaa atacacgtca ggtgttctgt
     5161 cgttcgcgga gaaggagctg ctgccgggag gacgtgaaaa agtgatggcg agctttgagc
     5221 gtgtactgat gcccggtctc gaaaaaaatc agtacagcat cctgtgggtg gagcaccagg
     5281 acaagggacg gcttgagctg aattttgtca ttccgaacat ggagctacag agcggaaaac
     5341 gcctccagcc gtactacgac cgggcagaca ggccgagaat tgatgcctgg cagacgctgg
     5401 ttaatcacca ttacgggctg catgacccca acgccccgga gaaccgccgg atactgactc
     5461 tccctgataa cctgccggaa acgaaacagg cgcttgcaga gagcgtcaca cgcgggatag
     5521 acgctcttta tcatgtcggg gagataaaag gccgtcagga tgtgattcag gcgctcacgg
     5581 aggcagggat tgaagtggtc agggtgacgc gaagcagtat cagcattgca gacccgaacg
     5641 gcgggaagaa tatcagactg aaaggagcat tctatgagca atcttttaca gacgggcgcg
     5701 gagttcgaga aaaagctgaa agagagagcc gaatctacag agacaatgct gaacgacgag
     5761 ttcagcaagc tcgaaaaatc tgtaagcaag gctgtgacat caaacgagac gaaaatcaaa
     5821 gacgctatag ccctgttcac agcttcgaca gaggaatcac tgaaaaaaca ccgggaaggg
     5881 gtgaaagagg cgatgatgca gcacaggaag gacgtgttaa agctggcagg gaatacgggc
     5941 atgatgttac tggggatggt cattttcctg tttaccgtga gtggcgggac gctctggtat
     6001 cttggcggga tgatacaggc gaacctggaa gaaatcagga agcagaacga gaatctggag
     6061 aagctgaacg cgaagacatg gggcgtgcgt tatcacgagg ggagcaacgg acgttttctg
     6121 gtgttgcccg aggggatgaa agccgaaacg aactggacga tggaaaacgg gaaacagaat
     6181 gccgtaaggc tggtacagga gtaacagagc atgacggagc tggaaagaca tttgctgaac
     6241 gcattagagc aactgcaaca ggactatatg cagcggctga acgaatggga gagcgccttc
     6301 gtggaattgc agaaaatgtt ttcgcttacg caacgggaca acgcgatgct gaacgagcgg
     6361 gtcatgcagt tgagtcagca ggtgcagcac ttgagcgagc agacagaacg cttgagccag
     6421 ttatacagcg agaacaggag ataagggacg aacggctgat acaggaacgc gaacatgcgt
     6481 tatccctgga gcgtgagcgc cagccggaaa tacaggaacg gacactggat ggcccttcgc
     6541 tgggatggta atcagaggca ggctaaaatg ttgtcagcgc gtttccggtg cattttttgg
     6601 gataatggtt ggttcggggg caccgggaac gcgcttcagt cagaccttgc tgaaaacaca
     6661 ggaacgcgtt aaaacgcata cgggcgcgat tctggtgtgt cttttgttgc tgtgttttgc
     6721 ctctattgcc agatcgcgct cattcccctg aacttctgtt ttcccgctcc ggtccccgtc
     6781 ttcttcgctt ccgaaagcat gaaaaaaatc cgtttcagct gtctctggct ggcaattcct
     6841 gcgtgattca catggctgca tagctatgca tgtggttaaa atttaccggg tgcgatcgcg
     6901 gcagtttttc gggtggtttg ttgccatttt tacctgtctg ctgccgtgat cgcgccgaac
     6961 gcgtttcagc ggtgcgtaca attaagggat tatggtaaat ccacttactg tcttccctcg
     7021 tagccatcga gataaatcgc agaaatcgtg tcagccagca gcttcatttt ttccggtgat
     7081 ggcatatcac tgttcgtgta ttttctgccc gcaaaggtgg ttgtggttct cagtgactga
     7141 aggaattctt cagagtggaa ttcttttatg cgttgctgtg ccgtttctgc tgatactccg
     7201 gctgc
//
