LOCUS       CP057793                4074 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB14-C23 plasmid pRHB14-C23_4, complete
            sequence.
ACCESSION   CP057793
VERSION     CP057793.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148116
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH04 T1-C23
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 86.8079007503833x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 11:38:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,090
            CDSs (total)                      :: 4,973
            Genes (coding)                    :: 4,772
            CDSs (with protein)               :: 4,772
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 201
            CDSs (without protein)            :: 201
            Pseudo Genes (ambiguous residues) :: 0 of 201
            Pseudo Genes (frameshifted)       :: 80 of 201
            Pseudo Genes (incomplete)         :: 118 of 201
            Pseudo Genes (internal stop)      :: 36 of 201
            Pseudo Genes (multiple problems)  :: 31 of 201
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB14-C23"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB14-C23_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(2392..4074,1..477)
                     /locus_tag="HVW99_25420"
     CDS             join(2392..4074,1..477)
                     /locus_tag="HVW99_25420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QMM64981.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     gene            complement(735..1427)
                     /locus_tag="HVW99_25425"
     CDS             complement(735..1427)
                     /locus_tag="HVW99_25425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QMM64982.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1959..2213)
                     /locus_tag="HVW99_25430"
     CDS             complement(1959..2213)
                     /locus_tag="HVW99_25430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QMM64983.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKTEVTRQADVTRILPLINELEALPGQ"
                     /name="mobilization protein"
     Typing          complement(2215..2391)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          2392..2750
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.778"
                     /coverage="100.0"
                     /evalue="1.83e-175"
     Typing          complement(736..1427)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.821"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2306..4074
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.492"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcaaggaagg tggattttac gcagggacgt ctgccaccga cgctgatgcc ggtaatgagt
       61 tctgtcgacc gggaaacccg ccagcttcag cttttgctgg tcatgggcgt ggatgactct
      121 ctgggcggtg ttgtccggct taatggtacg ctgtatcccg cgtttgccgt tccctcagcc
      181 gataacagcc agctggtcat cagtgcactg accgataagg ggttacggta tgcagggtat
      241 ggtgttgccg taaatcacga tgcagacagc cacatatcac ctgcgccgga actaatggag
      301 tttcatctga aaacccgtga ggcaccgctt tttgctgccg tcaacacacc agaaaaacag
      361 ccggatcatc tgttccgctc gctgggtttc aatcgtacct gggacgaatg gcggcgcgag
      421 gaagatgcaa gaacacacac caccgagagg agacatgatc gcggttggtc acagtgaatg
      481 tgaacaatat tttgttttgt ttaacatcgt gttatgttcg tcgatgttct tcaatgttca
      541 tcgacaggga tgaatgcaag aagcacactc acataacgca agcatagttt tgtttttccg
      601 cccccaaaaa ttgggggttc ttttttcgct gcatcagagc gtttttgacg caatttttca
      661 cttcaatagt accttcgcac tactaacaca aaaaaaacgc agcagggagc gttttaggtg
      721 ccttaaaatc gattttagat cgtcatcttg atctgtttgt cttccttgaa gcgaaaatgc
      781 agggctacaa ccgtgcgccc ctttttgatc gtctcaaccg ttacagccag gtcgcttttt
      841 ttgttcagtt cgtcaacaca gggttttatt agtattttat ttaagtctct gaattgtttg
      901 tatttatttt caatccctaa cattgaacga aagtcattaa gagcaataat tctctctccc
      961 gtactgcgga attgctggag gaactcgtat attcttatac tgtaactact tgataaacct
     1021 gatatgtttt ttataacaac gcgcgtgaat tgccccttca gttgcgtcag ataaggcatt
     1081 accgcgtcag aaaaggttat tcgagccttg gcctcacctt tagcgtactg tgctcgatac
     1141 tggatccagc ggaactcctc acgcttctcg tcattcttga gaatgatcga cctgtcccat
     1201 aaacgatcga tcgcttcctg taactgaacc tcggcatttt tccgccccat gtcagggaat
     1261 gaatcaaaat attccgcagc ggtaatggtc attgtctttt gcaattcagc atcagcacca
     1321 gatttcaatc gactgataca cagcaacaga aaacgctgtt cctgtagggt caacttgtag
     1381 ctggcctcaa ccaaagcatt cgacttgtag gcaatcaagt ccgacataca aagatcccgt
     1441 tcaaatgtgg tgatgcttta atgtgcccca caaataagaa atgtggaatg ttttttttta
     1501 tatcctcaca tttctgtgaa taacacctta aattcctcca cctttacctt aaattcctcc
     1561 acctaaacac cttaaattcc tccaccttta ccttaaattc ctccacatat accactataa
     1621 catattgatt ttattaatga atttcacccc taaaacaagt attaaaaaca agatcttaaa
     1681 aacaagatct aaaaacttaa cggcgagcct gtggataact tggggtgcta aaaacaaaaa
     1741 accaataaaa acaatgtgtt gcaagaaagc agaattgcaa gttccgaata ctccccctcc
     1801 aaccctgtgg aaatccagta ttcatgcggg ttcagcgcat atcacctgcc gaaaaaccgt
     1861 gcgtatgtgc tcaacctggc gacggattca cttccgcttc ctcgcccact cgctcgctaa
     1921 cgctcggtcg ctggggctgc ggcgagcgga cgatacaatc actgtcctgg cagcgcctcc
     1981 agctcgttaa tcagcggcag tatgcgcgtc acatcagcct gtcgtgtcac ctctgtcttc
     2041 aggaactgga gtaaccaggc acgcaactgc acctcctccg gctttctgac ctttgccagc
     2101 aatgctgcac ccacaacaac cttctgcgct gtatccaggc tccggctctt cgccttcagg
     2161 cgctgtaatc tcgcctcagc atcagcaatc ttctgttcga gcgttctgct catgatgtaa
     2221 ctccgtgcgc gatgaaaaat cgcattttag cgcgccactg gtagtttaaa aactgagctg
     2281 gcataatgca cgacaaatca cgaagtgcgc acttatacaa tctccactcc gtttcgattg
     2341 tgtgcgccct gcggggctga aagaaaacta caaaaaggca ttacggcaga aatggcgatt
     2401 tatcatctca gcatgaaaat tatctccaga agcagcggct acagcgccgt agcttccgct
     2461 gcctatcgtt ccggttcact gatgcttgat gagcgtaccg gactgactca cgactacacc
     2521 cgcaaaagcg gtgtggctga agctgtgatt ctgacccccg cgacagcccc tgcttggtgt
     2581 acgaaccgtg ccgaactctg gaatgccgtt gagaaggccg aacgccggaa gaactcacaa
     2641 cttgcgcggg aaattgaact cgctatcccc cgtgaactgc cgcaggatgc cgcacgggaa
     2701 accgttctcg cctttgtcag agaaaatttt gtcagtcagg gcatgattgc tgatgtggcg
     2761 ttccatcaca tggataaaac aaatccccat gcgcacatca tgctgaccac gagggctgtc
     2821 ggacctgctg ggttcggcgg aaaggtcagg gactggaacg accggacaca tgccgagacg
     2881 tggcgtgcat catgggctga ccatgcaaac agagcactgg cgaacgccgg ttatcaggaa
     2941 gagatagacc atcgttcata cgagcgacag gggcttgaga aaacgcctgg tattcacctc
     3001 ggaaagtctg cctgtgcgat ggaaacacgc ggtatcgaaa cagaacgcgg cgaacagaat
     3061 cgtctgatta acaggcttaa cctggaaata cagatttccc gcactgaact caggaacagc
     3121 gggctgacag agcctgcccg ccgtgtggct gaccttctga atatcgtgct tcctgacaac
     3181 gcaacttcgt acacactacg tgacatcatc gaagcgttgc cgaaggacag taatgcggca
     3241 tggaaactca ccagtaatgc catgtcgatg atggcagata tgcaggcgac gcgcagacgc
     3301 tgggaagagc tgaacaccga acgaaaggaa gcgatgagcc atgctgccag cctgaaaaaa
     3361 tcatcgccgt tttcgtccgg tttttcccgt attcccctga tgcaatgggc ggcaccggaa
     3421 taccgtagcg aacagggaaa aatccagtcg ctgagccagc aaatggaaaa attgcgacag
     3481 cattaccgga aggtggagaa acaggacatt cccgcgagtc agacggcatt tgaaaaacag
     3541 tggcagcagt ggggggcatc cggccttgca gctctcagag tccagttgct taaacgtgaa
     3601 gaggaattgc gccggaagga gcaggaggat acagaacgta gtcgcgcgga gcgacgagcg
     3661 gaacagctca ggaaaaatga caatgccgtt atggattcag gggtactgag cgcagtcgtt
     3721 acggggtatg gcgaggcccc catgcctggt aacggggaaa tgacctgtta ccttctgctt
     3781 cacaaccgga acggagaata tacgctctgg gggaacgagc tggagaaata ccgcaccaga
     3841 atctttgagc gtgttgattt aatgcgtgac aggagtggtt atatctgtga ccgaagcgaa
     3901 atcggtcaac atccaccact gcaacgtgtt tattcctctg cgacctttga gcaacttctg
     3961 acgcaggttt gccagacatg gccgcaatat acccgcaatc tccgccagcc gaagacatgg
     4021 ccggagtctt tctgtcttgg agaagaccgc cagccagcca tgccatcact ggca
//
