LOCUS       CP057781                6930 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB15-C18 plasmid pRHB15-C18_4, complete
            sequence.
ACCESSION   CP057781
VERSION     CP057781.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148124
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH11 T2-C18
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 89.425503819059x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 11:28:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,965
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,725
            CDSs (with protein)               :: 4,725
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 50 of 117
            Pseudo Genes (incomplete)         :: 53 of 117
            Pseudo Genes (internal stop)      :: 32 of 117
            Pseudo Genes (multiple problems)  :: 18 of 117
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB15-C18"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB15-C18_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(6794..6930,1..286))
                     /gene="exc2"
                     /locus_tag="HVX07_24775"
     CDS             complement(join(6794..6930,1..286))
                     /gene="exc2"
                     /locus_tag="HVX07_24775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMM50958.1"
                     /translation="MRSAAVRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFHTQKADTIRMMHPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEEFLQSLRTTTT
                     FAGRKYTNSDMPSPEKMKLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            408..686
                     /locus_tag="HVX07_24780"
     CDS             408..686
                     /locus_tag="HVX07_24780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMM50950.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLASLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(678..968)
                     /locus_tag="HVX07_24785"
     CDS             complement(678..968)
                     /locus_tag="HVX07_24785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMM50951.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            1209..2708
                     /gene="csa"
                     /locus_tag="HVX07_24790"
     CDS             1209..2708
                     /gene="csa"
                     /locus_tag="HVX07_24790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /protein_id="QMM50952.1"
                     /translation="MAKELSGYGPTAGESVGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGGEHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPGAGGNSDVQG
                     GYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEK
                     AKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDNLPPNVRKFDVDVEQF
                     HYLVVLDDYGNVLSITRTGVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEEPQRQ
                     NQAKKAAYDVFYSFSMNRDRIQSDVLNKAAEVISDIGNKVGDYLGNAYKSLAREIADDV
                     KNFQGKTIRSYDDAMASLNKVLSNPGFKFNRADSDALANAWRSIDAQDMANKLGNISKA
                     FKFADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASAVALGVFSATLGAYAL
                     SLGAPAIAVGIVGILLAAVVGALIDDKFADALNNEIIKPAH"
                     /name="csa"
     gene            complement(2736..3275)
                     /gene="csi"
                     /locus_tag="HVX07_24795"
     CDS             complement(2736..3275)
                     /gene="csi"
                     /locus_tag="HVX07_24795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 immunity protein"
                     /protein_id="QMM50953.1"
                     /translation="MMIDEHSIDIDNRKANNLLYLFMVIGVIPLLCILAVYYTNPDNLF
                     LHTIATSTENIPSITSAYNPLMTKVMDIYCKTAPSLALILFILTFKTRKLIKKTNRNAV
                     LRSCLLSPFVCAVFLYLLCFRNLELTTAGRPARLMANNDATLLLFYIGLYLIIFFISYF
                     TLFTPVTTFKLLKERQ"
                     /name="csi"
     gene            3305..3460
                     /gene="csl"
                     /locus_tag="HVX07_24800"
     CDS             3305..3460
                     /gene="csl"
                     /locus_tag="HVX07_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="QMM50954.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GSSGSL"
                     /name="csl"
     gene            complement(4459..4650)
                     /locus_tag="HVX07_24805"
     CDS             complement(4459..4650)
                     /locus_tag="HVX07_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMM50955.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4715..5038
                     /locus_tag="HVX07_24810"
     CDS             4715..5038
                     /locus_tag="HVX07_24810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMM50959.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5028..5738
                     /locus_tag="HVX07_24815"
                     /pseudo=""
     CDS             5028..5738
                     /locus_tag="HVX07_24815"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5716..6234
                     /locus_tag="HVX07_24820"
     CDS             5716..6234
                     /locus_tag="HVX07_24820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QMM50956.1"
                     /translation="MSNLLQTGVEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIKGAIAQFTASTEESLKKHREGVKEVMMQHRKDMLKLAGNTGMRLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVLPYGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            6241..6474
                     /gene="mbeD"
                     /locus_tag="HVX07_24825"
     CDS             6241..6474
                     /gene="mbeD"
                     /locus_tag="HVX07_24825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMM50957.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="mbeD"
     Typing          4287..4374
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          complement(3718..4264)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.171"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5029..6578
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.603"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggaattcttc agagtggaat tcttttacac gttgctgtgc cgtttctgct gatactccgg
       61 ctgctttgtc tttcatcccc atatcccgga actgctgaaa gaacgggtgc atcatacgta
      121 tggtgtctgc tttctgggtg tggaagttag gatcaaatcc atcatctgaa gcataaacat
      181 aatgccgggt atgtcgttcc aggctggtct ttggtgtgca tcctgtaaga gctattgttg
      241 ctgttatcag aggtatcaga agcagcctga cagcggcaga tctcataaac atctccttgc
      301 gtttttaatc ttgatactca gggagttata tctccgaaga gtatctctta tcatacgtcg
      361 gtttactccc aatgtgattt gatggtcaat gagtcaggaa taatcccgtg aaaaaatgtg
      421 tgccagtcct gctttttagt gttctttctg cttcggttgc cagtcatgcg gacagcaatg
      481 tttttcaaca aaaggccgag aagacaaaaa acggcattgt tgcggatgtt aatgccagac
      541 agaaaatgtt cagtaacaga ctgaagatac aacttcagat cgatgagttg agagcagagc
      601 tggctggcac cagagatgcc caaaaaagag aggatttgcg taaaaaactg gcgtctctgg
      661 agcggcaaaa gaaatcctta cgctgatggg ggcgtatttt ccccggtttc ttccataacc
      721 tttttaccgt tcagatattt acgggcgagg cgaatgcgtc ttgcctggta taccgccgag
      781 taagcctggg tgggattcac accgttactg atttcactga ccacatgacg ctgatcagcc
      841 agtgtcagcg tgtaaacctg tttctggatg aggtcacggg ctttgtcatg ctcatatccg
      901 gcagcaacca gggtgtcgag aagtcgcttg tctgttttgt tcagtttatg gcggggagat
      961 ttgctcacat cgttctccgt tttttcatga tccaacagtt ttatatctca tcctgatttg
     1021 ctccggtgtt ctgtttcgac gttctcaatg tctgcctccc catagtgtga tccttattgc
     1081 aaaaatgaaa atccatgctc ttgacatgga taatgctgag tagtaggttt ttgctgaata
     1141 caaaaccagt ggttatatgt acagtatatt gcttttaatt tattgtttta attatcaaag
     1201 aggaatttat ggctaaagaa ctaagtggat atggcccaac tgctggtgag tctgtggggg
     1261 gaacaggggc gaatctgaat cagcaaggtg gaaataataa tagtaatagt ggggttcact
     1321 ggggtggtgg ttcaggctct ggtaatggag gtggtgaaca tggaagtcag acggggtggg
     1381 gatggagtaa aaccaataat ccggatgtgc cgccttatgt tgatgataat gggcaggtgc
     1441 gcattaccat cacaaatggt ctggtaaaaa cacctgtata tggagttcct ggggctggcg
     1501 ggaatagtga tgttcagggg ggctatattc ctgaaaatcc taatgatgaa gttgctcgta
     1561 agtgggataa aaacaactta ccgcgagaga ttgatgtgag tatcgatggt tttaaatatc
     1621 gtgtaacgct taatgataat ggtagagcta tcggtattct tcgaacaggt gtccgtcctt
     1681 acgtagggag tgaaaaagct aaggcgggta ttatggaaaa aattaatcat aaaacacctg
     1741 aggagatata tgaggcgtta ggttttaata aagatgaatc tcagcggcag gaaaaagcaa
     1801 aacaacaggc tgaagatgcg tgggataatt tgcctcctaa tgttagaaaa tttgatgttg
     1861 atgttgaaca gttccactat ttagtggtgc ttgatgatta tggtaatgtt ttatctataa
     1921 ctagaacagg tgttcgtcct tatgtaggga gtgaaaaagc taaggctggt attatggata
     1981 aagtcgacca taagaccccg gaagagatat atgaggcatt aggatttaat aatgaagagc
     2041 ctcaacgaca gaatcaagct aaaaaagctg cttatgatgt gttttattct ttcagtatga
     2101 accgtgaccg gattcagagt gatgtactta ataaagctgc tgaagttatc tctgatatag
     2161 gtaataaggt cggtgattat cttggaaatg cttataagtc gttagcaaga gaaattgctg
     2221 atgatgtcaa aaatttccag gggaaaacca tccgtagcta cgatgatgca atggcgtcat
     2281 taaataaagt tttgtctaat cctgggttta aatttaacag ggccgacagt gatgcactgg
     2341 cgaacgcctg gcgaagtatt gatgctcagg atatggcaaa taaactgggt aatatcagca
     2401 aggctttcaa atttgccgat gtggtgatga aggttgagaa ggttcgggag aaaagcatcg
     2461 agggctatga aaccgggaac tggggacctc tgatgctgga ggtggagtcc tgggtgctca
     2521 gtggcattgc ttctgctgtg gccctggggg ttttttcagc cacattaggg gcatatgcct
     2581 tatctcttgg ggctcctgct attgctgtcg gtatcgtagg tattctactc gcagcagttg
     2641 taggtgcatt aattgatgac aagtttgcag atgcactaaa taacgaaatc attaaacccg
     2701 ctcattaata aataccgggt aaaaggaagg cagggttact gcctttcttt taataattta
     2761 aacgtggtaa ctggtgtgaa tagcgtgaaa tagcttataa aaaaaatgat taagtacagg
     2821 ccaatataaa atagcaatag tgttgcatca ttgttggcca ttagcctagc tggccttcct
     2881 gctgtcgtta gctcaaggtt tcggaagcag agtaaataca aaaaaacagc acagacaaat
     2941 ggacttaata agcaacttct aagtacagca tttcggtttg ttttttttat tagttttctc
     3001 gttttaaatg tcaggataaa taatatcaaa gcaagggaag gcgctgtttt acaataaata
     3061 tccataacct ttgtcattaa tgggttgtag gcggaggtta ttgatgggat gttttcagta
     3121 ctggtagcta ttgtgtgtaa gaacagattg tctggatttg tatagtaaac ggctagtata
     3181 cagagtaaag gtataactcc gattaccata aataaataca ataagttatt ggcttttcta
     3241 ttgtctatat ctattgaatg ttcatcaatc atcattgacc tataggatta taataaaggt
     3301 atatatgaaa aaaattatta tttgtgccat ttcactagga ataatgctgc ttgcagcatg
     3361 ccaagtcaac aatgtcagag atactggagg tggggctgtt tcaccatctt ccactgttac
     3421 cggagtgagt atgggaagtg gtagtagtgg tagcctgtaa aggatcttct tgagatcctt
     3481 tacagatcgt cgtagtctct tgccctgtaa acgaaaaaac cgcctgggga ggcggtttag
     3541 tcgaaggtta agtcagttgg ggactgctta accgggtagc tggcttaagt ggagcacaga
     3601 taccaaatgc tgtcctttca gtgtagccgc tataaggctt caacttcaag agttctgcgt
     3661 atctaaatcc acttttcagt taccaatggc tgctgccagt ggcgctttgt cgtgtcttac
     3721 cgggttggac tcgagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3781 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gggccgagat accaacagcg
     3841 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3901 cggcagggtc ggaacaggag agcgcacgag ggagcttccg gggggaaacg cctggtatct
     3961 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4021 aggggggcgg agcctatgga aaaacgccag tggcgcggcc tttttttgtc ctttgccttt
     4081 ttactcacat gttctttccg gccttatccc ctgattctgt ggataaccgt atttccgcct
     4141 tttagtgagc cggcaccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     4201 aggaagcgga agagtgttgt aagttgcatt ttctccttac gcatctgtgc ggcatttcac
     4261 accgtccatg gcgcactttt catacaatcc gcatcgatgc cgcatagtta agccagtata
     4321 cactccgcta tcgctacgtg actgggtcag ggctgcgccc cgacacccgc taaaacctgc
     4381 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct cacaggcaag ctgtgaccgt
     4441 ctccgggagc tgcatgtgtc agaggttttc accgtcatcc ccgaaacgtg cgaggcagct
     4501 gcggtaaagc tcatcggcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc
     4561 gtccagttcg ttgagctttt ccagcagcgt taatgtctgg cttctgataa agcgggccat
     4621 gttaagggcg gttttttcct gtttagtcac tgatgcctcc gtgtaagggg gatttctgtt
     4681 catgggggta atgataccga tgaaacgcga gaggatgctc acgatacggg ttactgatga
     4741 tgaacatgcc cggttactgg aacgctgtga gggtaaacaa ctggcggtat ggatgcggcg
     4801 ggtctgtctg ggtgagccgg ttgcccgttc cgggaaactg ccgacactgg caccgccgtt
     4861 actgcgtcag ctggccgcca tcggaaataa cctgaatcag acagcccgta aggtgaacag
     4921 cgggcagtgg tcttccggtg accgggttca ggtggtggcc gcactgatgg ccatcgggga
     4981 cgagctgcgc cggctgcgtc tggccgtcag ggaacagggg acgcgggatg atagttaaat
     5041 ttcatgccag gggaaaaggt ggtggcagtg gtccggttga ttacctgctg gggagggagc
     5101 gtaaccgcga aggcgcaacg gtgcttcagg gtaatccgga agaagtccgg gaactcatcg
     5161 atgccacgcc atttgcgaag aaatacacgt caggcgttct gtcgttcgcg gagaaggagc
     5221 tgccgccggg aggacgtgaa aaagtgatgg cgagctttga gcgcgtactg atgcccggtc
     5281 tcgaaaagaa tcagtacagc atcctgtggg tggagcacca ggacaaggga cggcttgagc
     5341 tgaattttgt cattccgaac atggagctac agaccggaaa acgcctccag ccgtactacg
     5401 accgggcaga caggccgaga attgatgcct ggcagacgct ggtgaatcac cattacgggc
     5461 tgcatgaccc caacgccccg gagaaccgcc ggatactgac actccctgat aacctgccgg
     5521 aaacgaaaca ggcgcttgca gagagcgtca cacgcgggat agacgctctt tatcatgtcg
     5581 gggagataag aggccgtcag gatgtgattc aggcgctcac agaggcaggg cttgaagtgg
     5641 tcagggtgac gcgaagcagt atcagcattg cagacccgaa cggcgggaag aatatcaggc
     5701 tgaaaggagc attttatgag caatctttta cagacgggcg tggagttcga gaaaaagctg
     5761 aaagagagag ccgaatctac agagaaaatg ctgaacgacg aattcaggaa gctcggcgaa
     5821 tctgtaagcg aggctgtgac ctcaaacgag acgaaaatca aaggcgctat agcccagttt
     5881 acggcctcga cggaggaatc gctgaaaaaa caccgggaag gggtgaaaga ggtgatgatg
     5941 cagcaccgga aggacatgtt aaagctggca gggaatacgg gcatgaggtt gctggggata
     6001 gtctttctcc tgtttaccgc gagtggcggg acgctctggt atcttggcgg gcggatacag
     6061 gcgaacctgg aagaaatcag gatacaggaa gagacattgc agaaactgaa cgcgaagaca
     6121 tggggcgtgg agtttgtgca ggacgggaga aggaaatttc tggtccttcc gtacgggaaa
     6181 tcggcaacgg tgattcccta tcaggggaag gactgggtac aactgacgga gtgacacaga
     6241 gtgacggagc tggaaagaca tttgctgaac gcattagagc aactgcaaca ggactatatg
     6301 cagcggctga gcgaatggga gagcgccttc gtggaattgc agaaaatgtt ttcgcttacg
     6361 caacgggaca acgcgatgct gaacgagcag gtcatgcagt tgagtcagca ggtgcagcac
     6421 ttgagcgagc ggacagaacg cttgagccag ttatacagcg agaacaggag ataagggacg
     6481 aacggctgat acaggaacgc gaacatgcgt tatccctgga acgggagcgt cagccggaaa
     6541 tacaggaacg cacgctggat ggtccttcgc tgggatggtg aaaccatgaa aaatggcagc
     6601 ttcagtggat taagaggggt taatgtggcc tgtaccatct ggttgcatag gtattcatgc
     6661 ggttaaaatt tatcaggcgc gatcgcggca gtttttcggg tggtttgttg ccatttttac
     6721 ctgtctgctg ccgtgatcgc gctgaacgcg tttcagcggt gcgtacaata agggattatg
     6781 gtaaatgtct cccttactgc ctgccctcgt agccatcgag ataaaccgca gaaatcgtgt
     6841 cagccagcag cttcattttt tccggtgatg gcatatcact gttcgtgtat tttctgcccg
     6901 caaaggtggt tgtggttctc agtgactgaa
//
