LOCUS       CP057776                6930 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB15-C22 plasmid pRHB15-C22_4, complete
            sequence.
ACCESSION   CP057776
VERSION     CP057776.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148126
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH11 T2-C22
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 88.4500427730931x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 11:20:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,958
            CDSs (total)                      :: 4,841
            Genes (coding)                    :: 4,740
            CDSs (with protein)               :: 4,740
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 43 of 101
            Pseudo Genes (incomplete)         :: 48 of 101
            Pseudo Genes (internal stop)      :: 19 of 101
            Pseudo Genes (multiple problems)  :: 8 of 101
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB15-C22"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB15-C22_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            19..1518
                     /gene="csa"
                     /locus_tag="HVX09_24725"
     CDS             19..1518
                     /gene="csa"
                     /locus_tag="HVX09_24725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /protein_id="QMM46223.1"
                     /translation="MAKELSGYGPTAGESVGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGGEHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPGAGGNSDVQG
                     GYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEK
                     AKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDNLPPNVRKFDVDVEQF
                     HYLVVLDDYGNVLSITRTGVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEEPQRQ
                     NQAKKAAYDVFYSFSMNRDRIQSDVLNKAAEVISDIGNKVGDYLGNAYKSLAREIADDV
                     KNFQGKTIRSYDDAMASLNKVLSNPGFKFNRTDSDALANAWRSIDAQDMANKLGNISKA
                     FKFADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASAVALGVFSATLGAYAL
                     SLGAPAIAVGIVGILLAAVVGALIDDKFADALNNEIIKPAH"
                     /name="csa"
     gene            complement(1546..2085)
                     /gene="csi"
                     /locus_tag="HVX09_24730"
     CDS             complement(1546..2085)
                     /gene="csi"
                     /locus_tag="HVX09_24730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 immunity protein"
                     /protein_id="QMM46224.1"
                     /translation="MMIDEHSIDIDNRKANNLLYLFMVIGVIPLLCILAVYYTNPDNLF
                     LHTIATSTENIPSITSAYNPLMTKVMDIYCKTAPSLALILFILTFKTRKLIKKTNRNAV
                     LRSCLLSPFACAVFLYLLCFRNLELTTAGRPARLMANNDATLLLFYIGLYLIIFFISYF
                     TLFTPVTTFKLLKERQ"
                     /name="csi"
     gene            2115..2270
                     /gene="csl"
                     /locus_tag="HVX09_24735"
     CDS             2115..2270
                     /gene="csl"
                     /locus_tag="HVX09_24735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="QMM46225.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GSSGSL"
                     /name="csl"
     gene            complement(2486..2599)
                     /locus_tag="HVX09_24740"
     CDS             complement(2486..2599)
                     /locus_tag="HVX09_24740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QMM46226.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAAIGN"
                     /name="replication initiation protein"
     gene            complement(3269..3460)
                     /locus_tag="HVX09_24745"
     CDS             complement(3269..3460)
                     /locus_tag="HVX09_24745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMM46227.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3525..3848
                     /locus_tag="HVX09_24750"
     CDS             3525..3848
                     /locus_tag="HVX09_24750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMM46233.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3838..4548
                     /locus_tag="HVX09_24755"
                     /pseudo=""
     CDS             3838..4548
                     /locus_tag="HVX09_24755"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4526..5044
                     /locus_tag="HVX09_24760"
     CDS             4526..5044
                     /locus_tag="HVX09_24760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QMM46228.1"
                     /translation="MSNLLQTGVEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIKGAIAQFTASTEESLKKHREGVKEAMMQHRKDMLKLAGNTGMRLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVLPYGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            5051..5284
                     /gene="mbeD"
                     /locus_tag="HVX09_24765"
     CDS             5051..5284
                     /gene="mbeD"
                     /locus_tag="HVX09_24765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMM46229.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="mbeD"
     gene            complement(5604..6026)
                     /gene="exc2"
                     /locus_tag="HVX09_24770"
     CDS             complement(5604..6026)
                     /gene="exc2"
                     /locus_tag="HVX09_24770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMM46230.1"
                     /translation="MRSAAVRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFHTQKADTIRMMHPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEEFLQSLRTTTT
                     FAGRKYTNSDMPSPEKMKLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            6148..6426
                     /locus_tag="HVX09_24775"
     CDS             6148..6426
                     /locus_tag="HVX09_24775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMM46231.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKSGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLASLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(6418..6708)
                     /locus_tag="HVX09_24780"
     CDS             complement(6418..6708)
                     /locus_tag="HVX09_24780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMM46232.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     Typing          3097..3184
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          complement(2528..3074)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.171"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3839..5388
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.603"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attatcaaag aggaatttat ggctaaagaa ctaagtggat atggcccaac tgctggtgag
       61 tctgtggggg gaacaggggc gaatctgaat cagcaaggtg gaaataataa tagtaatagt
      121 ggggttcact ggggtggtgg ttcaggctct ggtaatggag gtggtgaaca tggaagtcag
      181 acggggtggg gatggagtaa aaccaataat ccggatgtgc cgccttatgt tgatgataat
      241 gggcaggtgc gcattaccat cacaaatggt ctggtaaaaa cacctgtata tggagttcct
      301 ggggctggcg ggaatagtga tgttcagggg ggctatattc ctgaaaatcc taatgatgaa
      361 gttgctcgta agtgggataa aaacaactta ccgcgagaga ttgatgtgag tatcgatggt
      421 tttaaatatc gtgtaacgct taatgataat ggtagagcta tcggtattct tcgaacaggt
      481 gtccgtcctt acgtagggag tgaaaaagct aaggcgggta ttatggaaaa aattaatcat
      541 aaaacacctg aggagatata tgaggcgtta ggttttaata aagatgaatc tcagcggcag
      601 gaaaaagcaa aacaacaggc tgaagatgcg tgggataatt tgcctcctaa tgttagaaaa
      661 tttgatgttg atgttgaaca gttccactat ttagtggtgc ttgatgatta tggtaatgtt
      721 ttatctataa ctagaacagg tgttcgtcct tatgtaggga gtgaaaaagc taaggctggt
      781 attatggata aagtcgacca taagaccccg gaagagatat atgaggcatt aggatttaat
      841 aatgaagagc ctcaacgaca gaatcaagct aaaaaagctg cttatgatgt gttttattct
      901 ttcagtatga accgtgaccg gattcagagt gatgtactta ataaagctgc tgaagttatc
      961 tctgatatag gtaataaggt cggtgattat cttggaaatg cttataagtc gttagcaaga
     1021 gaaattgctg atgatgtcaa aaatttccag gggaaaacca tccgtagcta cgatgatgca
     1081 atggcgtcat taaataaagt tttgtctaat cctgggttta aatttaacag gaccgacagt
     1141 gatgcactgg cgaacgcctg gcgaagtatt gatgctcagg atatggcaaa taaactgggt
     1201 aatatcagca aggctttcaa atttgccgat gtggtgatga aggttgagaa ggtccgggag
     1261 aaaagcatcg agggctatga aaccgggaac tggggacctc tgatgctgga ggtggagtcc
     1321 tgggtgctca gtggcattgc ttctgctgtg gccctggggg ttttttcagc cacattaggg
     1381 gcatatgcct tatctcttgg ggctcctgct attgctgtcg gtatcgtagg tattctactc
     1441 gcagcagttg taggtgcatt aattgatgac aagtttgcag atgcactaaa taacgaaatc
     1501 attaaacccg ctcattaata aataccgggt aaaaggaagg cagggttact gcctttcttt
     1561 taataattta aacgtggtaa ctggtgtgaa tagcgtgaaa tagcttataa aaaaaatgat
     1621 taagtacagg ccaatataaa atagcaatag tgttgcatca ttgttggcca ttagcctagc
     1681 tggccttcct gctgtcgtta gctcaaggtt tcggaagcag agtaaataca aaaaaacagc
     1741 acaggcaaat ggacttaata agcaacttct aagtacagca tttcggtttg ttttttttat
     1801 tagttttctc gttttaaatg tcaggataaa taatatcaaa gcaagggaag gcgctgtttt
     1861 acaataaata tccataacct ttgtcattaa tgggttgtag gcggaggtta ttgatgggat
     1921 gttttcagta ctggtagcta ttgtgtgtaa gaacagattg tctggatttg tatagtaaac
     1981 ggctagtata cagagtaaag gtataactcc gattaccata aataaataca ataagttatt
     2041 ggcttttcta ttgtctatat ctattgaatg ttcatcaatc atcattgacc tataggatta
     2101 taataaaggt atatatgaaa aaaattatta tttgtgccat ttcactagga ataatgctgc
     2161 ttgcagcatg ccaagtcaac aatgtcagag atactggagg tggggctgtt tcaccatctt
     2221 ccactgttac cggagtgagt atgggaagtg gtagtagtgg tagcctgtaa aggatcttct
     2281 tgagatcctt tacagatcgt cgtagtctct tgccctgtaa acgaaaaaac cgcctgggga
     2341 ggcggtttag tcgaaggtta agtcagttgg ggactgctta accgggtagc tggcttaagt
     2401 ggagcacaga taccaaatgc tgtcctttca gtgtagccgc tataaggctt caacttcaag
     2461 agttctgcgt atctaaatcc acttttcagt taccaatggc tgctgccagt ggcgctttgt
     2521 cgtgtcttac cgggttggac tcgagacgat agttaccgga taaggcgcag cggtcgggct
     2581 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gggccgagat
     2641 accaacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     2701 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttccg gggggaaacg
     2761 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     2821 gatgctcgtc aggggggcgg agcctatgga aaaacgccag cggcgcggcc tttttttgtc
     2881 ctttgccttt ttactcacat gttctttccg gccttatccc ctgattctgt ggataaccgt
     2941 atttccgcct tttagtgagc cggcaccgct cgccgcagcc gaacgaccga gcgcagcgag
     3001 tcagtgagcg aggaagcgga agagtgtcgt aagttgcatt ttctccttac gcatctgtgc
     3061 ggcatttcac accgtccatg gcgcactttt catacaatcc gcatcgatgc cgcatagtta
     3121 agccagtata cactccgcta tcgctacgtg actgggtcag ggctgcgccc cgacacccgc
     3181 taaaacctgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct cacaggcaag
     3241 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatcc ccgaaacgtg
     3301 cgaggcagct gcggtaaagc tcatcggcgt ggtcgtgaag cgattcacag atgtctgcct
     3361 gttcatccgc gtccagttcg ttgagctttt ccagcagcgt taatgtctgg cttctgataa
     3421 agcgggccat gttaagggcg gttttttcct gtttagtcac tgatgcctcc gtgtaagggg
     3481 gatttctgtt catgggggta atgataccga tgaaacgcga gaggatgctc acgatacggg
     3541 ttactgatga tgaacatgcc cggttactgg aacgctgtga gggtaaacaa ctggcggtat
     3601 ggatgcggcg ggtctgtctg ggtgagccgg ttgcccgttc cgggaaactg ccgacactgg
     3661 caccgccgtt actgcgtcag ctggccgcca tcggaaataa cctgaatcag acagcccgta
     3721 aggtgaacag cgggcagtgg tcttccggtg accgggttca ggtggtggcc gcactgatgg
     3781 ccatcgggga cgagctgcgc cggctgcgtc tggccgtcag ggaacagggg acgcgggatg
     3841 atagttaaat ttcatgccag gggaaaaggt ggtggcagtg gtccggttga ttacctgctg
     3901 gggagggagc gtaaccgcga aggcgcaacg gtgcttcagg gtaatccgga agaagtccgg
     3961 gaactcatcg atgccacgcc atttgcgaag aaatacacgt caggcgttct gtcgttcgcg
     4021 gagaaggagc tgccgccggg aggacgtgaa aaagtgatgg cgagctttga gcgcgtactg
     4081 atgcccggtc tcgaaaagaa tcagtacagc atcctgtggg tggagcacca ggacaaggga
     4141 cggcttgagc tgaattttgt cattccgaac atggagctac agaccggaaa acgcctccag
     4201 ccgtactacg accgggcaga caggccgaga attgatgcct ggcagacgct ggtgaatcac
     4261 cattacgggc tgcatgaccc caacgccccg gagaaccgcc ggatactgac actccctgat
     4321 aacctgccgg aaacgaaaca ggcgcttgca gagagcgtca cacgcgggat agacgctctt
     4381 tatcatgtcg gggagataag aggccgtcag gatgtgattc aggcgctcac agaggcaggg
     4441 cttgaagtgg tcagggtgac gcgaagcagt atcagcattg cagacccgaa cggcgggaag
     4501 aatatcaggc tgaaaggagc attttatgag caatctttta cagacgggcg tggagttcga
     4561 gaaaaagctg aaagagagag ccgaatctac agagaaaatg ctgaacgacg aattcaggaa
     4621 gctcggcgaa tctgtaagcg aggctgtgac ctcaaacgag acgaaaatca aaggcgctat
     4681 agcccagttt acggcctcga cggaggaatc gctgaaaaaa caccgggaag gggtgaaaga
     4741 ggcgatgatg cagcaccgga aggacatgtt aaagctggca gggaatacgg gcatgaggtt
     4801 gctggggata gtctttctcc tgtttaccgc gagtggcggg acgctctggt atcttggcgg
     4861 gcggatacag gcgaacctgg aagaaatcag gatacaggaa gagacattgc agaaactgaa
     4921 cgcgaagaca tggggcgtgg agtttgtgca ggacgggaga aggaaatttc tggtccttcc
     4981 gtacgggaaa tcggcaacgg tgattcccta tcaggggaag gactgggtac aactgacgga
     5041 gtgacacaga gtgacggagc tggaaagaca tttgctgaac gcattagagc aactgcaaca
     5101 ggactatatg cagcggctga gcgaatggga gagcgccttc gtggaattgc agaaaatgtt
     5161 ttcgcttacg caacgggaca acgcgatgct gaacgagcag gtcatgcagt tgagtcagca
     5221 ggtgcagcac ttgagcgagc ggacagaacg cttgagccag ttatacagcg agaacaggag
     5281 ataagggacg aacggctgat acaggaacgc gaacatgcgt tatccctgga acgggagcgt
     5341 cagccggaaa tacaggaacg cacgctggat ggtccttcgc tgggatggtg aaaccatgaa
     5401 aaatggcagc ttcagtggat taagaggggt taatgtggcc tgtaccatct ggttgcatag
     5461 gtattcatgc ggttaaaatt tatcaggcgc gatcgcggca gtttttcggg tggtttgttg
     5521 ccatttttac ctgtctgctg ccgtgatcgc gctgaacgcg tttcagcggt gcgtacaata
     5581 agggattatg gtaaatgtct cccttactgc ctgccctcgt agccatcgag ataaaccgca
     5641 gaaatcgtgt cagccagcag cttcattttt tccggtgatg gcatatcact gttcgtgtat
     5701 tttctgcccg caaaggtggt tgtggttctc agtgactgaa ggaattcttc agagtggaat
     5761 tcttttacac gttgctgtgc cgtttctgct gatactccgg ctgctttgtc tttcatcccc
     5821 atatcccgga actgctgaaa gaacgggtgc atcatacgta tggtgtctgc tttctgggtg
     5881 tggaagttag gatcaaatcc atcatctgaa gcataaacat aatgccgggt atgtcgttcc
     5941 aggctggtct ttggtgtgca tcctgtaaga gctattgttg ctgttatcag aggtatcaga
     6001 agcagcctga cagcggcaga tctcataaac atctccttgc gtttttaatc ttgatactca
     6061 gggagttata tctccgaaga gtatctctta tcatacgtcg gtttactccc aatgtgattt
     6121 gatggtcaat gagtcaggaa taatcccgtg aaaaaatgtg tgccagtcct gctttttagt
     6181 gttctttctg cttcggttgc cagtcatgcg gacagcaatg tttttcaaca aaaggccgag
     6241 aagacaaaaa gcggcattgt tgcggatgtt aatgccagac agaaaatgtt cagtaacaga
     6301 ctgaagatac aacttcagat cgatgagttg agagcagagc tggctggcac cagagatgcc
     6361 caaaaaagag aggatttgcg taaaaaactg gcgtctctgg agcggcaaaa gaaatcctta
     6421 cgctgatggg ggcgtatttt ccccggtttc ttccataacc tttttaccgt tcagatattt
     6481 acgggcgagg cgaatgcgtc ttgcctggta taccgccgag taagcctggg tgggattcac
     6541 accgttactg atttcactga ccacatgacg ctgatcagcc agtgtcagcg tgtaaacctg
     6601 tttctggatg aggtcacggg ctttgtcatg ctcatatccg gcagcaacca gggtgtcgag
     6661 aagtcgcttg tctgttttgt tcagtttatg gcggggagat ttgctcacat cgttctccgt
     6721 tttttcatga tccaacagtt ttatatctca tcctgatttg ctccggtgtt ctgtttcgac
     6781 gttctcaatg tctgcctccc catagtgtga tccttattgc aaaaatgaaa atccatgctc
     6841 ttgacatgga taatgctgag tagtaggttt ttgctgaata caaaaccagt ggttatatgt
     6901 acagtatatt gcttttaatt tattgtttta
//
