LOCUS       CP057765                6669 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB16-C07 plasmid pRHB16-C07_7, complete
            sequence.
ACCESSION   CP057765
VERSION     CP057765.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148133
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6669)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH02 T2-C07
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 62.6311386817588x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 12:33:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,057
            CDSs (total)                      :: 5,933
            Genes (coding)                    :: 5,709
            CDSs (with protein)               :: 5,709
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 69 of 224
            Pseudo Genes (incomplete)         :: 132 of 224
            Pseudo Genes (internal stop)      :: 42 of 224
            Pseudo Genes (multiple problems)  :: 15 of 224
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB16-C07"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB16-C07_7"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(182..601)
                     /gene="exc2"
                     /locus_tag="HVX16_30210"
     CDS             complement(182..601)
                     /gene="exc2"
                     /locus_tag="HVX16_30210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMM36524.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(682..1119)
                     /locus_tag="HVX16_30215"
     CDS             complement(682..1119)
                     /locus_tag="HVX16_30215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QMM36525.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            1379..2947
                     /gene="cea"
                     /locus_tag="HVX16_30220"
     CDS             1379..2947
                     /gene="cea"
                     /locus_tag="HVX16_30220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="QMM36526.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(2944..3285)
                     /locus_tag="HVX16_30225"
     CDS             complement(2944..3285)
                     /locus_tag="HVX16_30225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="QMM36527.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            3333..3470
                     /locus_tag="HVX16_30230"
     CDS             3333..3470
                     /locus_tag="HVX16_30230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QMM36528.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(3669..3827)
                     /locus_tag="HVX16_30235"
     CDS             complement(3669..3827)
                     /locus_tag="HVX16_30235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QMM36529.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(4497..4688)
                     /locus_tag="HVX16_30240"
     CDS             complement(4497..4688)
                     /locus_tag="HVX16_30240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMM36530.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4753..5076
                     /locus_tag="HVX16_30245"
     CDS             4753..5076
                     /locus_tag="HVX16_30245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMM36533.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5018..5776
                     /locus_tag="HVX16_30250"
                     /pseudo=""
     CDS             5018..5776
                     /locus_tag="HVX16_30250"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5754..6272
                     /locus_tag="HVX16_30255"
     CDS             5754..6272
                     /locus_tag="HVX16_30255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbeB"
                     /protein_id="QMM36531.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="mobilization protein MbeB"
     gene            6279..6533
                     /gene="mbeD"
                     /locus_tag="HVX16_30260"
     CDS             6279..6533
                     /gene="mbeD"
                     /locus_tag="HVX16_30260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMM36532.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSEQTERLSQLYSENWR"
                     /name="mbeD"
     Typing          4325..4412
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          complement(3756..4311)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.52"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5067..6637
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.809"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taagtggggg taatgtggcc tgtaccctct ggttgcatag gtattcatac ggttaaaatt
       61 tatcaggcgc gatcgcggca gtttttcggg tggtttgttg ccatttttac ctgtctgctg
      121 ccgtgatcgc gctgaacgcg ttttagcggt gcgtacaatt aagggattat ggtaaatcca
      181 cttactgtct gccctcgtag ccatcgagat aaaccgcaga aatcgtgtca gccagcagcc
      241 tggatttttt cggggtaagg tctttgctgc ggtaatcagt acccgcaaat tgagttgtgc
      301 cccggagtga gtttaaaaat tcctggctgt gaaactgctg aatgcgttgt tgcacatcac
      361 tccgtgattt tcctgtcgct ttatctttag ctcccatatc ccagaactgc cgaaaaaaag
      421 gcaccatcat tcgtgttgtg tcggcttttt gggtggaaaa gttgggatca aaaccgtcat
      481 cagaagcata tacgtaatgc cgggtatgtt gttccacgct gccctttggg gtacaggctg
      541 tgagagctac tgttgctgtt atcatggcga taagacaaag tttgtttccg gtacgtctca
      601 tgagtgtctc cctaccttaa agtatttatt ctcggtgagt tattatccga agactatttt
      661 atcatcagtc tctgacagcg ttcagcgacc gaacaacctg gaccagaatc cccgtttctt
      721 ttccagttcc agtgcctgcc tgagctgggc tatctcattt tgtagctgtt cccgttctgc
      781 ttcctggcgt ctgcgatcca tatcctgtgc ctgtttatcc tcaagcatca gcgtcaggca
      841 ttgtttcagc tcattcagct cccggagaat gcgttctgtc tgctgatcat gtggattttc
      901 tgcatgtccc tcactgtgcc tttctggtgt ctcattctgc tttaattcgc cgtatgcccg
      961 gatcagttca ctggtttcaa actcccgtcg tccgtccctg ccggtacggt aactgacaag
     1021 gcctgcgcgc atatcacggt aaaactgtga acgcgatctg cctgtcattt ttagtgcgtc
     1081 ccggagtgtg tgccatgcca taaagtgaca gtgtcccata gatgtctcat ctcatagttt
     1141 cagtaaaaca taatgaggtc tgagaacggt aatgtttgtg ctggtttttg tggcatcggg
     1201 cgagaatagc gcgtggtgtg aaagactgtt tttttgatcg ttttcacaaa aatggaagtc
     1261 cacagtcttg acagggaaaa tgcagcggcg tagcttttat gctgtatata aaaccagtgg
     1321 ttatatgtac agtatttatt tttaacttat tgttttaaaa gtcaaagagg attttataat
     1381 ggaaaccgcg gtagcgtact ataaagatgg tgttccttat gatgataagg gacaggtaat
     1441 tattactctt ttgaatggta ctcctgacgg gagtggctct ggcggcggag gtggaaaagg
     1501 aggcagtaaa agtgaaagtt ctgcagctat tcatgcaact gctaaatggt ctactgctca
     1561 attaaagaaa acacaggcag agcaggctgc ccgggcaaaa gctgcagcgg aagcacaggc
     1621 gaaagcaaag gcaaacaggg atgcgctgac tcagcgcctg aaggatatcg tgaatgaggc
     1681 tcttcgtcac aatgcctcac gtacgccttc agcaacagag cttgctcatg ctaataatgc
     1741 agctatgcag gcggaagcag agcgtttgcg ccttgcgaaa gcagaagaaa aagcccgtaa
     1801 agaagcggaa gcagcagaaa aggcttttca ggaagcagaa caacgacgta aagagattga
     1861 acgggagaag gctgaaacag aacgccagtt gaaactggct gaagctgaag agaaacgact
     1921 ggctgcattg agtgaagaag ctaaagctgt tgagatcgcc caaaaaaaac tttctgctgc
     1981 acaatctgaa gtggtgaaaa tggatggaga gattaagact ctcaattctc gtttaagctc
     2041 cagtatccat gcccgtgatg cagaaatgaa aacgctcgct ggaaaacgaa atgaactggc
     2101 tcaggcatcc gctaaatata aagaactgga tgagctggtc aaaaaactat caccaagagc
     2161 caatgatccg cttcagaacc gtcctttttt tgaagcaacc agacgacggg ttggggccgg
     2221 taagattaga gaagaaaaac aaaaacaggt aacagcatca gaaacacgta ttaaccggat
     2281 aaatgctgat ataactcaga tccagaaggc tatttctcag gtcagtaata atcgtaatgc
     2341 cggtatcgct cgtgttcatg aagctgaaga aaatttgaaa aaagcacaga ataatctcct
     2401 taattcacag attaaggatg ctgttgatgc aacagttagc ttttatcaaa cgctgactga
     2461 aaaatatggt gaaaaatatt cgaaaatggc acaggaactt gctgataagt ctaaaggtaa
     2521 gaaaatcggc aatgtgaatg aagctctcgc tgcttttgaa aaatacaagg atgttttaaa
     2581 taagaaattc agcaaagccg atcgtgatgc tatttttaat gcgttggcat cggtgaagta
     2641 tgatgactgg gctaaacatt tagatcagtt tgccaagtac ttgaagatta cggggcatgt
     2701 ttcttttgga tatgatgtgg tatctgatat cctaaaaatt aaggatacag gtgactggaa
     2761 gccactattt cttacattag agaagaaagc tgcagatgca ggggtgagtt atgttgttgc
     2821 tttacttttt agcttgcttg ctggaactac attaggtatt tggggtattg ctattgttac
     2881 aggaattcta tgctcctata ttgataagaa taaacttaat actataaatg aggtgttagg
     2941 gatttaatta ttctttattg atataaaaag tcctagcaat ccaaatggga ttgctaggac
     3001 caaacaaagt agattatata gcataaatag gtttaatttt gctacggggg cgttatttag
     3061 gttttttctt ctttcgaaaa aatctttctt tatgaagtta aaagctatgt attcaatagc
     3121 atattttgaa tatggacata gaatagtgct tatcactatt gcatatagca tcttatctga
     3181 cacaaggaaa taataccctt cgctgttttt tgttataagg tatatatata taagtgtgca
     3241 gtacaggcca aataaaatat tttttatgta gtatcttaag ctcataaatt aaacctcgcc
     3301 atatattctt ttcattttat aaggatcgag ttatgaggaa aagatttttt gtgggaatat
     3361 tcgcgataaa cctccttgtt ggatgtcagg ctaactatat acgtgatgtt cagggaggga
     3421 ccatcgcacc atcctcctct tctaaactga cggggatcgc ggttcagtag aaaagatcaa
     3481 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3541 accgttacca acggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3601 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagtcggg
     3661 ccactacttc aagaactctg tagcaccgtt tgtgccatca tcgctctgct aatccggtta
     3721 ccagtggctg ctgccagtgg cgttaaggcg tgccttaccg ggttggactc aagacgatag
     3781 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3841 gagcgaacga cctacaccga actgagatac caacagcgtg agctatgaga aagcgccacg
     3901 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3961 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4021 cacctctgac ttgagcgtct atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4081 aacgcctgct acgtggcctt cttcctgttc ttggtctttt gctcacatgt tctttccggc
     4141 cttatcccct gattctgtgg ataactgtgt taccgttttt gtgtgagtca gtaccgctcg
     4201 ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaaa agcgcctgga
     4261 cgtgcatttt ctccttacgc atctgtgcgg catttcacac ccggcatggc gtacttttca
     4321 tacaatccgc actaatgccg catggttaag ccagtataca ctccgctatc gctacgtgac
     4381 tgggtcaggg ctgcgccccg acacccgcta aaacctgctg acgcgccctg acgggcttgt
     4441 ctgctcccgg catccgctca cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     4501 aggttttcac cgtcatcccc gaaacgtgcg aggcagctgc ggtaaagctc atcggcgtgg
     4561 tcgtgaagcg attcacagat gtctgcctgt tcatctgcgt ccagttcgtt gagcttctcc
     4621 agcagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt
     4681 ttagtcactg atgcctccgt gtaagggggt atttctgttc atggggtaat gataccgatg
     4741 aaacgcgaga ggatgctcac gatacgggtt actgatgatg aacatgccag gttacttgaa
     4801 cgctgtgagg gtaaacaact ggcagtatgg atgcggcggg tctgcctggg tgagccggtt
     4861 gcccgttccg ggaagctgcc gacactggca ccgccgttac tgcgtcagct ggccgccatc
     4921 gggaataacc tgaatcagac agcgaggaag gtgaacagcg ggcagtggtc ttccggtgac
     4981 cgggttcagg tggtggccgc actgatggcc atcggggatg agctgcgccg gctgcgtctg
     5041 gccgtcaggg aacagggggc gcgggatgat agttaaattt catgccaggg gaaaaggtgg
     5101 tggcagtggt ccggttgatt acctgctggg gagggagcgt aaccgcgaag gcgcaacggt
     5161 gcttcagggt aatccggaag aagtccggga actcatcgat gccacgccat ttgcgaagaa
     5221 atacacgtcc ggtgttctgt cgttcgcgga gaaggagctg ccgccgggag gacgtgaaaa
     5281 agtgatggcg agctttgagc gtgtactgat gcccggtctc gaaaaaaatc agtacagcat
     5341 cctgtgggtg gagcaccagg acaagggacg gcttgagctg aattttgtca ttccgaacat
     5401 ggagctacag accggaaaac gcctccagcc gtactacgac cgcgcagaca ggcctagaat
     5461 tgatgcctgg cagacgctgg tgaatcacca ttacgggctg catgacccga acgccccgga
     5521 gaaccgcagg acgctgacac tccctgataa cctgcctgaa acgaaacagg cgcttgctga
     5581 gggcgtcacg cgaggtatag atgcacttta ccatgccgga gagataaaag gccgtcagga
     5641 tgtgattcag gcgctcactg aggcggggct ggaagtggtc agggtgacgc gaagcagtat
     5701 cagcattgca gatccgaacg gcgggaagaa tatcaggctg aaaggagcat tttatgagca
     5761 atcttttgca gacgggcgcg gagttcgaga aaaagctgaa agagagagcc gaatctacag
     5821 agaaaatgct gaacaacgag ttcaggaggc tcggcgaatc tgtaagcgag gctgtgacat
     5881 caaacgagac gaaaatcaga gacgctatag ccctgttcac agcctcgaca gaggaatcgc
     5941 tggaaaaaca ccgggaaggg gtgaaagagg cgatgatgca gcacaggagg gacgtgttaa
     6001 agctggcagg gaatacgggc atgatgttac tggggatagt ctttctcctg tttaccgcga
     6061 gtggcgggac gctctggtat cttggaggga ggatacaggc gaacctggaa gaaatcagga
     6121 agcaggaaga gacattgcag aaactgaacg cgaagacatg gggcgtggag tttgtgcagg
     6181 acgggaacag gaaattcctt gtccttccgt acgggaaatc agcggaggtg attccctttc
     6241 aggggaaaga gtgggtacat ctgaaggagt gacacagagt gacagagctg gaaacacatt
     6301 tgctgaacgc cttagagcag ctgcaacagg actatatgca gcggctgagc gaatgggaga
     6361 gcgccttcgt ggaattgcag aagatgtttt cgcttacgca acgggacaac gcgatgctga
     6421 acgagcgggt catgcagttg agtcagcagg tgcagcactt gagcgagcag acagaacgct
     6481 tgagcgagca gacagaacgc ttgagccagt tatacagcga gaactggaga taagagagga
     6541 acggctgata caggagcgcg aacatgtgtt atccctggaa cgggagcgtc agccggaaat
     6601 acaggaacgc acgctggatg gcccttcgct gggatggtga aaccatgaaa aatggcagct
     6661 tcagtggat
//
