LOCUS       CP057757                6669 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB16-C08 plasmid pRHB16-C08_7, complete
            sequence.
ACCESSION   CP057757
VERSION     CP057757.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148134
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6669)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH02 T2-C08
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 74.1494556741556x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 12:30:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,063
            CDSs (total)                      :: 5,939
            Genes (coding)                    :: 5,713
            CDSs (with protein)               :: 5,713
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 226
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 226
            Pseudo Genes (frameshifted)       :: 70 of 226
            Pseudo Genes (incomplete)         :: 133 of 226
            Pseudo Genes (internal stop)      :: 42 of 226
            Pseudo Genes (multiple problems)  :: 15 of 226
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB16-C08"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB16-C08_7"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(56..247)
                     /locus_tag="HVX17_30240"
     CDS             complement(56..247)
                     /locus_tag="HVX17_30240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMM30815.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            312..635
                     /locus_tag="HVX17_30245"
     CDS             312..635
                     /locus_tag="HVX17_30245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMM30824.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            793..1335
                     /locus_tag="HVX17_30250"
                     /pseudo=""
     CDS             793..1335
                     /locus_tag="HVX17_30250"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1313..1831
                     /locus_tag="HVX17_30255"
     CDS             1313..1831
                     /locus_tag="HVX17_30255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbeB"
                     /protein_id="QMM30816.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="mobilization protein MbeB"
     gene            1838..2092
                     /gene="mbeD"
                     /locus_tag="HVX17_30260"
     CDS             1838..2092
                     /gene="mbeD"
                     /locus_tag="HVX17_30260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMM30817.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSEQTERLSQLYSENWR"
                     /name="mbeD"
     gene            complement(2410..2829)
                     /gene="exc2"
                     /locus_tag="HVX17_30265"
     CDS             complement(2410..2829)
                     /gene="exc2"
                     /locus_tag="HVX17_30265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMM30818.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(2910..3347)
                     /locus_tag="HVX17_30270"
     CDS             complement(2910..3347)
                     /locus_tag="HVX17_30270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QMM30819.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            3607..5175
                     /gene="cea"
                     /locus_tag="HVX17_30275"
     CDS             3607..5175
                     /gene="cea"
                     /locus_tag="HVX17_30275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="QMM30820.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(5172..5513)
                     /locus_tag="HVX17_30280"
     CDS             complement(5172..5513)
                     /locus_tag="HVX17_30280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="QMM30821.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            5561..5698
                     /locus_tag="HVX17_30285"
     CDS             5561..5698
                     /locus_tag="HVX17_30285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QMM30822.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(5897..6055)
                     /locus_tag="HVX17_30290"
     CDS             complement(5897..6055)
                     /locus_tag="HVX17_30290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QMM30823.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     Typing          6553..6640
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          complement(5984..6539)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.52"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          626..2196
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.809"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgctcccggc atccgctcac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
       61 ggttttcacc gtcatccccg aaacgtgcga ggcagctgcg gtaaagctca tcggcgtggt
      121 cgtgaagcga ttcacagatg tctgcctgtt catctgcgtc cagttcgttg agcttctcca
      181 gcagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
      241 tagtcactga tgcctccgtg taagggggta tttctgttca tggggtaatg ataccgatga
      301 aacgcgagag gatgctcacg atacgggtta ctgatgatga acatgccagg ttacttgaac
      361 gctgtgaggg taaacaactg gcagtatgga tgcggcgggt ctgcctgggt gagccggttg
      421 cccgttccgg gaagctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg
      481 ggaataacct gaatcagaca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc
      541 gggttcaggt ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg
      601 ccgtcaggga acagggggcg cgggatgata gttaaatttc atgccagggg aaaaggtggt
      661 ggcagtggtc cggttgatta cctgctgggg agggagcgta accgcgaagg cgcaacggtg
      721 cttcagggta atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa
      781 tacacgtccg gtgttctgtc gttcgcggag aaggagctgc cgccgggagg acgtgaaaaa
      841 gtgatggcga gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatc
      901 ctgtgggtgg agcaccagga caagggacgg cttgagctga attttgtcat tccgaacatg
      961 gagctacaga ccggaaaacg cctccagccg tactacgacc gcgcagacag gcctagaatt
     1021 gatgcctggc agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag
     1081 aaccgcagga cgctgacact ccctgataac ctgcctgaaa cgaaacaggc gcttgctgag
     1141 ggcgtcacgc gaggtataga tgcactttac catgccggag agataaaagg ccgtcaggat
     1201 gtgattcagg cgctcactga ggcggggctg gaagtggtca gggtgacgcg aagcagtatc
     1261 agcattgcag atccgaacgg cgggaagaat atcaggctga aaggagcatt ttatgagcaa
     1321 tcttttgcag acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga
     1381 gaaaatgctg aacaacgagt tcaggaggct cggcgaatct gtaagcgagg ctgtgacatc
     1441 aaacgagacg aaaatcagag acgctatagc cctgttcaca gcctcgacag aggaatcgct
     1501 ggaaaaacac cgggaagggg tgaaagaggc gatgatgcag cacaggaggg acgtgttaaa
     1561 gctggcaggg aatacgggca tgatgttact ggggatagtc tttctcctgt ttaccgcgag
     1621 tggcgggacg ctctggtatc ttggagggag gatacaggcg aacctggaag aaatcaggaa
     1681 gcaggaagag acattgcaga aactgaacgc gaagacatgg ggcgtggagt ttgtgcagga
     1741 cgggaacagg aaattccttg tccttccgta cgggaaatca gcggaggtga ttccctttca
     1801 ggggaaagag tgggtacatc tgaaggagtg acacagagtg acagagctgg aaacacattt
     1861 gctgaacgcc ttagagcagc tgcaacagga ctatatgcag cggctgagcg aatgggagag
     1921 cgccttcgtg gaattgcaga agatgttttc gcttacgcaa cgggacaacg cgatgctgaa
     1981 cgagcgggtc atgcagttga gtcagcaggt gcagcacttg agcgagcaga cagaacgctt
     2041 gagcgagcag acagaacgct tgagccagtt atacagcgag aactggagat aagagaggaa
     2101 cggctgatac aggagcgcga acatgtgtta tccctggaac gggagcgtca gccggaaata
     2161 caggaacgca cgctggatgg cccttcgctg ggatggtgaa accatgaaaa atggcagctt
     2221 cagtggatta agtgggggta atgtggcctg taccctctgg ttgcataggt attcatacgg
     2281 ttaaaattta tcaggcgcga tcgcggcagt ttttcgggtg gtttgttgcc atttttacct
     2341 gtctgctgcc gtgatcgcgc tgaacgcgtt ttagcggtgc gtacaattaa gggattatgg
     2401 taaatccact tactgtctgc cctcgtagcc atcgagataa accgcagaaa tcgtgtcagc
     2461 cagcagcctg gattttttcg gggtaaggtc tttgctgcgg taatcagtac ccgcaaattg
     2521 agttgtgccc cggagtgagt ttaaaaattc ctggctgtga aactgctgaa tgcgttgttg
     2581 cacatcactc cgtgattttc ctgtcgcttt atctttagct cccatatccc agaactgccg
     2641 aaaaaaaggc accatcattc gtgttgtgtc ggctttttgg gtggaaaagt tgggatcaaa
     2701 accgtcatca gaagcatata cgtaatgccg ggtatgttgt tccacgctgc cctttggggt
     2761 acaggctgtg agagctactg ttgctgttat catggcgata agacaaagtt tgtttccggt
     2821 acgtctcatg agtgtctccc taccttaaag tatttattct cggtgagtta ttatccgaag
     2881 actattttat catcagtctc tgacagcgtt cagcgaccga acaacctgga ccagaatccc
     2941 cgtttctttt ccagttccag tgcctgcctg agctgggcta tctcattttg tagctgttcc
     3001 cgttctgctt cctggcgtct gcgatccata tcctgtgcct gtttatcctc aagcatcagc
     3061 gtcaggcatt gtttcagctc attcagctcc cggagaatgc gttctgtctg ctgatcatgt
     3121 ggattttctg catgtccctc actgtgcctt tctggtgtct cattctgctt taattcgccg
     3181 tatgcccgga tcagttcact ggtttcaaac tcccgtcgtc cgtccctgcc ggtacggtaa
     3241 ctgacaaggc ctgcgcgcat atcacggtaa aactgtgaac gcgatctgcc tgtcattttt
     3301 agtgcgtccc ggagtgtgtg ccatgccata aagtgacagt gtcccataga tgtctcatct
     3361 catagtttca gtaaaacata atgaggtctg agaacggtaa tgtttgtgct ggtttttgtg
     3421 gcatcgggcg agaatagcgc gtggtgtgaa agactgtttt tttgatcgtt ttcacaaaaa
     3481 tggaagtcca cagtcttgac agggaaaatg cagcggcgta gcttttatgc tgtatataaa
     3541 accagtggtt atatgtacag tatttatttt taacttattg ttttaaaagt caaagaggat
     3601 tttataatgg aaaccgcggt agcgtactat aaagatggtg ttccttatga tgataaggga
     3661 caggtaatta ttactctttt gaatggtact cctgacggga gtggctctgg cggcggaggt
     3721 ggaaaaggag gcagtaaaag tgaaagttct gcagctattc atgcaactgc taaatggtct
     3781 actgctcaat taaagaaaac acaggcagag caggctgccc gggcaaaagc tgcagcggaa
     3841 gcacaggcga aagcaaaggc aaacagggat gcgctgactc agcgcctgaa ggatatcgtg
     3901 aatgaggctc ttcgtcacaa tgcctcacgt acgccttcag caacagagct tgctcatgct
     3961 aataatgcag ctatgcaggc ggaagcagag cgtttgcgcc ttgcgaaagc agaagaaaaa
     4021 gcccgtaaag aagcggaagc agcagaaaag gcttttcagg aagcagaaca acgacgtaaa
     4081 gagattgaac gggagaaggc tgaaacagaa cgccagttga aactggctga agctgaagag
     4141 aaacgactgg ctgcattgag tgaagaagct aaagctgttg agatcgccca aaaaaaactt
     4201 tctgctgcac aatctgaagt ggtgaaaatg gatggagaga ttaagactct caattctcgt
     4261 ttaagctcca gtatccatgc ccgtgatgca gaaatgaaaa cgctcgctgg aaaacgaaat
     4321 gaactggctc aggcatccgc taaatataaa gaactggatg agctggtcaa aaaactatca
     4381 ccaagagcca atgatccgct tcagaaccgt cctttttttg aagcaaccag acgacgggtt
     4441 ggggccggta agattagaga agaaaaacaa aaacaggtaa cagcatcaga aacacgtatt
     4501 aaccggataa atgctgatat aactcagatc cagaaggcta tttctcaggt cagtaataat
     4561 cgtaatgccg gtatcgctcg tgttcatgaa gctgaagaaa atttgaaaaa agcacagaat
     4621 aatctcctta attcacagat taaggatgct gttgatgcaa cagttagctt ttatcaaacg
     4681 ctgactgaaa aatatggtga aaaatattcg aaaatggcac aggaacttgc tgataagtct
     4741 aaaggtaaga aaatcggcaa tgtgaatgaa gctctcgctg cttttgaaaa atacaaggat
     4801 gttttaaata agaaattcag caaagccgat cgtgatgcta tttttaatgc gttggcatcg
     4861 gtgaagtatg atgactgggc taaacattta gatcagtttg ccaagtactt gaagattacg
     4921 gggcatgttt cttttggata tgatgtggta tctgatatcc taaaaattaa ggatacaggt
     4981 gactggaagc cactatttct tacattagag aagaaagctg cagatgcagg ggtgagttat
     5041 gttgttgctt tactttttag cttgcttgct ggaactacat taggtatttg gggtattgct
     5101 attgttacag gaattctatg ctcctatatt gataagaata aacttaatac tataaatgag
     5161 gtgttaggga tttaattatt ctttattgat ataaaaagtc ctagcaatcc aaatgggatt
     5221 gctaggacca aacaaagtag attatatagc ataaataggt ttaattttgc tacgggggcg
     5281 ttatttaggt tttttcttct ttcgaaaaaa tctttcttta tgaagttaaa agctatgtat
     5341 tcaatagcat attttgaata tggacataga atagtgctta tcactattgc atatagcatc
     5401 ttatctgaca caaggaaata atacccttcg ctgttttttg ttataaggta tatatatata
     5461 agtgtgcagt acaggccaaa taaaatattt tttatgtagt atcttaagct cataaattaa
     5521 acctcgccat atattctttt cattttataa ggatcgagtt atgaggaaaa gattttttgt
     5581 gggaatattc gcgataaacc tccttgttgg atgtcaggct aactatatac gtgatgttca
     5641 gggagggacc atcgcaccat cctcctcttc taaactgacg gggatcgcgg ttcagtagaa
     5701 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5761 aaaaaaccac cgttaccaac ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5821 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     5881 tagtcgggcc actacttcaa gaactctgta gcaccgtttg tgccatcatc gctctgctaa
     5941 tccggttacc agtggctgct gccagtggcg ttaaggcgtg ccttaccggg ttggactcaa
     6001 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6061 ccagcttgga gcgaacgacc tacaccgaac tgagatacca acagcgtgag ctatgagaaa
     6121 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6181 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6241 ggtttcgcca cctctgactt gagcgtctat ttttgtgatg ctcgtcaggg gggcggagcc
     6301 tatggaaaaa cgcctgctac gtggccttct tcctgttctt ggtcttttgc tcacatgttc
     6361 tttccggcct tatcccctga ttctgtggat aactgtgtta ccgtttttgt gtgagtcagt
     6421 accgctcgcc gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga agcggaaaag
     6481 cgcctggacg tgcattttct ccttacgcat ctgtgcggca tttcacaccc ggcatggcgt
     6541 acttttcata caatccgcac taatgccgca tggttaagcc agtatacact ccgctatcgc
     6601 tacgtgactg ggtcagggct gcgccccgac acccgctaaa acctgctgac gcgccctgac
     6661 gggcttgtc
//
