LOCUS       CP057750                6801 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Citrobacter freundii strain RHB16-C09 plasmid pRHB16-C09_5, complete
            sequence.
ACCESSION   CP057750
VERSION     CP057750.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148135
KEYWORDS    .
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6801)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH02 T2-C09
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 81.0763709409121x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 14:39:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,061
            CDSs (total)                      :: 4,944
            Genes (coding)                    :: 4,844
            CDSs (with protein)               :: 4,844
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 27 of 100
            Pseudo Genes (incomplete)         :: 70 of 100
            Pseudo Genes (internal stop)      :: 14 of 100
            Pseudo Genes (multiple problems)  :: 10 of 100
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6801
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="RHB16-C09"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:546"
                     /plasmid="pRHB16-C09_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(6319..6801,1..45))
                     /locus_tag="HVX18_25265"
                     /pseudo=""
     CDS             complement(join(6319..6801,1..45))
                     /locus_tag="HVX18_25265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /name="phosphotransferase"
     gene            complement(106..921)
                     /gene="sul2"
                     /locus_tag="HVX18_25270"
     ARG             complement(110..921)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            2110..2259
                     /locus_tag="HVX18_25275"
     CDS             2110..2259
                     /locus_tag="HVX18_25275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMM25112.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            complement(3037..3477)
                     /locus_tag="HVX18_25280"
     CDS             complement(3037..3477)
                     /locus_tag="HVX18_25280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QMM25113.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="entry exclusion protein 1"
     gene            3695..4084
                     /locus_tag="HVX18_25285"
     CDS             3695..4084
                     /locus_tag="HVX18_25285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMM25114.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            4128..4532
                     /locus_tag="HVX18_25290"
                     /pseudo=""
     CDS             4128..4532
                     /locus_tag="HVX18_25290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(4639..5475)
                     /locus_tag="HVX18_25295"
     CDS             complement(4639..5475)
                     /locus_tag="HVX18_25295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="QMM25115.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(5475..5750)
                     /locus_tag="HVX18_25300"
                     /pseudo=""
     CDS             complement(5475..5750)
                     /locus_tag="HVX18_25300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
                     /name="aminoglycoside phosphotransferase APH(3')"
     gene            complement(5825..6298)
                     /locus_tag="HVX18_25305"
     CDS             complement(5825..6298)
                     /locus_tag="HVX18_25305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="QMM25116.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVGR
                     KTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEIYR
                     ETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
                     /name="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
     ARG             complement(4643..5475)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(5829..6298)
                     /name="dfrA14"
                     /gene="dfrA14"
                     /gene_name="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /tool="amrfinderplus"
                     /drug_class="TRIMETHOPRIM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(6305..6801)
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.38"
                     /coverage="61.8"
     ARG             complement(5476..5720)
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="30.34"
     Typing          1902..2075
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(1373..1978)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ccagtcagaa tgcgattcac caaaaaaaat attagttcga ttcaatggag gttccttcag
       61 ttttctgatg aagcgcgaat atagagaaat atcccgaatg tgcagttaac gaattcttgc
      121 ggtttctttc agcgccgcca ataccgccag cccgtcgcgc aaggggcgcg gctcgtgtgt
      181 gcggatgaag tcagctccac ctgcggcggc ggcaagctct gcagcgagtg tcgcggcccc
      241 gacatccccc ggaccacggc ctgtgagcgc gcgcagaaag gatttgcgcg aaacagacag
      301 aagcaccggc aaatcgaagc gcagccgcaa ttcatcgaac cgcgccagca ccgagagcga
      361 ggtttcggga gcagccccca gaaaaaaccc catgccggga tcaaggacaa ggcggttgcg
      421 tttgataccg gcacccgtca gcgccgcgat gcgcgcgtca aagaacgccg caatgtgatc
      481 catgatgtcg ccagcgggtg cctcgcgccg atctgcctgc ccgtcttgca ccgaatgcat
      541 aacgacgagt ttggcagatg atttcgccaa ttgcggatag aacgcagcgt ctggaaaacc
      601 gcgaatatca ttgagatagg ccacaccacg cgacaaggca taggcttgcg tcgcgggttg
      661 ataactgtcg agcgagacgg gaatgccatc tgccttgagc gcgtccagca ccggcgcgat
      721 acgcgcgatt tctgtgtcgg acgaaacagg cgcggcgtcg ggattgctgg atgccggacc
      781 gaggtcgatc acatctgccc cctcggccat cagcttacgc gcctgcgcaa tggctgcgtc
      841 tggcgccaga taccggcctc catcggagaa actgtccgag gttatgttga cgatgccgaa
      901 aatgatgagc gatttattca tgggggcttc tataataata ataatcgagc atgagtctca
      961 tacggatgct cgggtcgaaa gggaatcccc aggcgagtaa cctgtttgcg gtgatccgtt
     1021 tcatgatgat gtatctgtag cattttggaa ataaatatat catctctcaa taagtagata
     1081 tatttatctt ttttgcttct ttggtgctgc aagtcaccaa tacgagggaa acctgttacg
     1141 gagcggttta accgtaatca acagtgactg gtgtcatgtt cggaagatta attgatagta
     1201 atctacaaag gatcttcttg agatcctttt caatgaatcg taatctcttg ctctgtaaac
     1261 gaaaaaaccg cctggggagg cggtttgttc gaaggttaag tcagttgggg aactgcttaa
     1321 cccggtaaca gggttcacga gaccgccatt accaaaactg ttctttcagt gtagccgcag
     1381 tcaggcctct acttcaagac ctcttaatct atcttctcgt gcactcctgt tactaatggc
     1441 tgctgccagt ggcgttttgt cgtgtctttc cgggttggac tcaagttgat agttaccgga
     1501 caaggcgcag cggtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac
     1561 tgccttcacg gaactgagtg tcaggcgtgg aatgagaaac cgcggccata acagcggaat
     1621 gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagctac cagggggaaa
     1681 cgcctgggat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagatttt
     1741 gcgatgcttg tcaggggggc ggagcctatg gaaaaacggc ttcgccgtgg ccttatctct
     1801 tctttgctaa gtgtcttcct ggtatcttcc aggaaatttc cgtccccgtc cgtaagccgg
     1861 taccgctcgc cgcagccgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaata
     1921 tctcctgtat cgcatattct gctgacgcgc cggtgctgcc ttttttctcc tgccacatga
     1981 agcacttcac tgattttcgc aatcgtggca acatagtaag ccagtataca ctccgctagc
     2041 gctacatgac tggttcaggg ctgcgccccg aaacccgcta acaacctctc agctactgga
     2101 tatcacccca tgccatctga atatgatgaa caactggatt cgactgatac gcagcttccc
     2161 ggcgaatatg acctttttga tttaatggcg caggttactg aagagaacct gcatgcccct
     2221 gtggattttg ggtcgcctgt cggccgggaa gcgttatagc tttttcgctt gcccgcgtac
     2281 gtggtaatac tccggtatta ccttttattt ctttattttc atgtaattac agtggatttt
     2341 taatacccgg gtattactcg tgaaaacgaa atattccttt tttttctttt aattcatgca
     2401 gttgcgatga aaatcgcggc tgcgttaggt gtatgaccat tttgaggttg aaaaataatc
     2461 aaccatggcg ggtgatttac gcaggttaag gggagatcgt gacaaaaaaa gcgttcctca
     2521 ctgtatcgtt catgcctgca tgaacgggtt attcttcctg actacagaat tacatcgctc
     2581 cttcagttca tacatcactt ttacatcact tatacatcac ttttacatca cttttacatc
     2641 acttatacat cacttttaca tcattccttc agctcatacg tcactttcat cctctgtgcc
     2701 atttgtacat cacccccttc ctgaacgtgc tgacagcggg ttttccggct gcgccggcaa
     2761 cgcttcacgg ccacgcaata aattgcgtcc ccgttcaccg ttttcggatg ggaagaacgc
     2821 cggaaacagg acttaccccg gctgaatggt gtgaatttcc gcgctatgca cttgtgcgca
     2881 tactcatgca tgccgtaaaa acagagcctg cgcgtttctg gcgggttttc gggtggtttg
     2941 ttgcctgttt taccggtttc ccgtcagaaa cgccctgagg gcctctcagg cggtgctgat
     3001 aacagatgtt atggtaaatc agagtcacct gcactgttac tctgaaccgg agcgaaacag
     3061 ccgcgaccag aatccccgct ttttttcctg tgccagcgcg tcagccagac ggctaatttc
     3121 ggcctgtagt tgttccctct cctcatttcg caggcggttt tctgcttccc gcgccatctt
     3181 gtcttcaagc attagggtaa cagtctgctg cagagcgtcc agtttctgct gaatgacgtc
     3241 tagctgagca ttttccgcct ggctaacgac agatttggag tgtccctcct tgtgcctttt
     3301 tggtgtctca gaagtgcgca gctcgccata ggcccgaatc agttcactgg tctcgaactc
     3361 ccggcgatca ttttttcctg tgcgatagct gacaaggcct ttagccatgt cacgatataa
     3421 ttgcgagcgc gactttcctg ttaatttcag tgtctcttga actgtgtgcc atgccataaa
     3481 agtgacagtg tctcctgtgt gtaccgtatt gtaacagcag aacatattac tgttttaaca
     3541 caaaaggtct gaggctgccc gtgaggcgtg aaaaatacac aaaaaacctg ttgacggtgt
     3601 ggttttgacg ggcgtagtat ttcgaatact gtatataaac acattgttat aaatacagtt
     3661 tttgctgttt ttaaaagtca aagaggataa ttttatgagt acagagttaa atagcggtac
     3721 tgtcggtggt tttggtcaca tgggaccaaa tgacccctac aatgatggtc atagcggctc
     3781 tggaagttcg ggaggtagtt ttaacggtgg tggtaatggt ggccatgata atagtagtgg
     3841 ttcatcttat actgcatcct ttggtctcat cactggccga cacccgataa ctgtcccagg
     3901 tacgaagaat attaattctg tcgatctttc tgctgtcatt aataacatta tacatcatga
     3961 aggtgcacgc aataacatgt acctagatac tgcgaacaat gtaagagtgg gagtgacggg
     4021 cactggctgg caatgtctag caacggcagg catttcggct gagggtaaaa gaactttccg
     4081 ctaagcgata gactgtatgt aaacacagta ttgcaaggac gcggaacatg cctcatgtgg
     4141 cggccaggac ggccagccgg gatcgggata ctggtcgtta ccagagccac cgacccgagc
     4201 aaacccttct ctatcagatc gttgacgagt attacccggc attcgctgcg cttatggcag
     4261 agcagggaaa ggaattgccg ggctatgtgc aacgggaatt tgaagaattt ctccaatgcg
     4321 ggcggctgga gcatggcttt ctacgggttc gctgcgagtc ttgccacgcc gagcacctgg
     4381 tcgctttcag ctgtaagcgt cgcggtttct gcccgagctg tggggcgcgg cggatggccg
     4441 aaagtgccgc cttgctggtt gatgaagtac tgcctgaaca acccatgcgt cagtgggtgt
     4501 tgagcttccc gtttcagctg cgtttcctgt ttggggtcgt ttgcgggaag gggcggaatc
     4561 ctacgctaag gctttggcca gcgatattct ccggtgagat tgatgtgttc ccaggggata
     4621 ggagaagtcg cttgatatct agtatgacgt ctgtcgcacc tgcttgatcg cggccgcgat
     4681 agctagatcg cgttgctcct cttctccatc cgcgttccaa gctgcggaaa ggcacccata
     4741 agcgtacgcc tggtcgagca ggcgacgcgg atcgacgtcc agcgcacgag agaatgcgtc
     4801 cgccatctgt gcaatgcgtc taggatcgag acaaaggtcg tctctgtcag ccggatcgta
     4861 gaacatattg gcggcgccaa agcccacttc accgaccaga ccgacgggat ctatcaccag
     4921 ccagccgcga ctggagaaca tgatgttttc atgatgcaga tcgccatgta gcccacgcag
     4981 ttccgaggca ttgctcatca tttgatcggc tataatcgcc gcgtggacgt agtcagtttg
     5041 acaacctgcg ttttgatcat cgcgcgcccg ctgaaacaaa gctgcaaagc gatcccggat
     5101 cgggagaagg gcagaaggca ggggttcctc agatgcggca tacagcttcg ccattagttc
     5161 cgctgcaatt tcggtcgcct ggtagtcgcc gtgctcggca acgatgtgag agagcattcg
     5221 ctccccggca tattcgagca acatcagatt gttctcacga ccgagcaacc ggactgctcc
     5281 cctcccattg cgccatacca gatagtcggc cccgcgcagt tcatcagcaa tgtcttctat
     5341 aggtttcaat cccttgacga ttgcaggagt cccgtctggc aatgaaactt tccaaacgag
     5401 gctggaaaag gtgtccgcaa tgagaacagg ttgcgaaacg tgccaatgag caggaaaaac
     5461 aggcggcatg aacatcaacc ccaagtcaga gggtccaatc gcagatagaa ggcaaggcgt
     5521 tcgcggtcgg gggcttcgat ccccaataca ttgaatagga cagcgaaggc gcgctctgct
     5581 tcatctggcg ctgcccagtt ctcttcggcg ttagcaatca tgagtgccaa atcggcatag
     5641 cgatctgctg ttccgagccg cccaaggtcg atcagacccg tgcattgaag agttttaggg
     5701 tccaccatga agttcggcat gcagggatca ccatggcaaa caaccatatc gtcgctatca
     5761 atcgcggaaa aaggcgtagt gcgcgaagcg aacgaagctt tttgccgtcg attgcatagc
     5821 tttgttaacc ctttttccaa atttgatagc aatagttaat gtttgaagta aagtgttgct
     5881 caaaaacaac ttcgaaggta tttggaatac tcgggaagaa aacatccccc tctggctcga
     5941 tgtcgatcgt cgataagtgg agcgtagagg ccatgggtaa tgtttctcgg taaatttctc
     6001 cgccaccaga cactataacg tgaccggtga attcagctag cctgtccatg gcctcttcga
     6061 ttgactgaaa tacaactaca ttgtcatcat ttgatgtcca acctgagcgg gtaacgaccg
     6121 cgtatttcct attggggagt gcgcccatag attcaaacgt cttgcgaccc accagaagcc
     6181 actgattgta ggtcaatgct ttaaaaagta gctgctcccc tttcgcggac cagggtatgt
     6241 ctggaccgca accaatcacg ccgtttttcg ctttcgcagc tatcaatgat actttcaagg
     6301 ttctcatcct gggttaacgg tgcgctcttg gtcgagccgc accggtagct ctcgttcgac
     6361 acgagccaaa agatcgagct gcggcgtact cttgtcctcg tccggtaaga agtcgggatt
     6421 gacggcattg cgggacacca catcaacggc gcgtccgaac attcgcgaca gcctgcgctc
     6481 aaacggacat tgatcaaccg ataggctgtg aacagcgcca agttgctgcc ccattgacgg
     6541 ccacgctttg agcaaatccg ctccagacag atcagccgcc ggtactcccg gaattgccgt
     6601 tatcaccaag catgcaccct cctgttcctc ctgccagttg atgacctcgg ggcaagccac
     6661 acctcgacct ttgagccaaa tgaggcggtc acgctctcca gcgagctcac cgcggcggga
     6721 agcaggtgcg attttcgcga aggcatgccc gtcaccacgt cgaaaaacaa aatcaccaga
     6781 ttctccgcct ctgacaggca a
//
