LOCUS       CP057744                6669 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB16-C11 plasmid pRHB16-C11_7, complete
            sequence.
ACCESSION   CP057744
VERSION     CP057744.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148136
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6669)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH02 T2-C11
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 74.0769376535989x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 13:02:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,063
            CDSs (total)                      :: 5,939
            Genes (coding)                    :: 5,713
            CDSs (with protein)               :: 5,713
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 226
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 226
            Pseudo Genes (frameshifted)       :: 70 of 226
            Pseudo Genes (incomplete)         :: 133 of 226
            Pseudo Genes (internal stop)      :: 42 of 226
            Pseudo Genes (multiple problems)  :: 15 of 226
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB16-C11"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB16-C11_7"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(6233..6669,1..1132))
                     /gene="cea"
                     /locus_tag="HVX19_30240"
     CDS             complement(join(6233..6669,1..1132))
                     /gene="cea"
                     /locus_tag="HVX19_30240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="QMM20266.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            1392..1829
                     /locus_tag="HVX19_30245"
     CDS             1392..1829
                     /locus_tag="HVX19_30245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QMM20258.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            1910..2329
                     /gene="exc2"
                     /locus_tag="HVX19_30250"
     CDS             1910..2329
                     /gene="exc2"
                     /locus_tag="HVX19_30250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMM20259.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(2647..2901)
                     /gene="mbeD"
                     /locus_tag="HVX19_30255"
     CDS             complement(2647..2901)
                     /gene="mbeD"
                     /locus_tag="HVX19_30255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMM20260.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSEQTERLSQLYSENWR"
                     /name="mbeD"
     gene            complement(2908..3426)
                     /locus_tag="HVX19_30260"
     CDS             complement(2908..3426)
                     /locus_tag="HVX19_30260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbeB"
                     /protein_id="QMM20261.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="mobilization protein MbeB"
     gene            complement(3404..4162)
                     /locus_tag="HVX19_30265"
                     /pseudo=""
     CDS             complement(3404..4162)
                     /locus_tag="HVX19_30265"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4104..4427)
                     /locus_tag="HVX19_30270"
     CDS             complement(4104..4427)
                     /locus_tag="HVX19_30270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMM20267.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4492..4683
                     /locus_tag="HVX19_30275"
     CDS             4492..4683
                     /locus_tag="HVX19_30275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMM20262.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5353..5511
                     /locus_tag="HVX19_30280"
     CDS             5353..5511
                     /locus_tag="HVX19_30280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QMM20263.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(5710..5847)
                     /locus_tag="HVX19_30285"
     CDS             complement(5710..5847)
                     /locus_tag="HVX19_30285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QMM20264.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            5895..6236
                     /locus_tag="HVX19_30290"
     CDS             5895..6236
                     /locus_tag="HVX19_30290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="QMM20265.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     Typing          complement(4769..4856)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          4867..5425
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.365"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2544..4114)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.809"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acttatcagc aagttcctgt gccattttcg aatatttttc accatatttt tcagtcagcg
       61 tttgataaaa gctaactgtt gcatcaacag catccttaat ctgtgaatta aggagattat
      121 tctgtgcttt tttcaaattt tcttcagctt catgaacacg agcgataccg gcattacgat
      181 tattactgac ctgagaaata gccttctgga tctgagttat atcagcattt atccggttaa
      241 tacgtgtttc tgatgctgtt acctgttttt gtttttcttc tctaatctta ccggccccaa
      301 cccgtcgtct ggttgcttca aaaaaaggac ggttctgaag cggatcattg gctcttggtg
      361 atagtttttt gaccagctca tccagttctt tatatttagc ggatgcctga gccagttcat
      421 ttcgttttcc agcgagcgtt ttcatttctg catcacgggc atggatactg gagcttaaac
      481 gagaattgag agtcttaatc tctccatcca ttttcaccac ttcagattgt gcagcagaaa
      541 gttttttttg ggcgatctca acagctttag cttcttcact caatgcagcc agtcgtttct
      601 cttcagcttc agccagtttc aactggcgtt ctgtttcagc cttctcccgt tcaatctctt
      661 tacgtcgttg ttctgcttcc tgaaaagcct tttctgctgc ttccgcttct ttacgggctt
      721 tttcttctgc tttcgcaagg cgcaaacgct ctgcttccgc ctgcatagct gcattattag
      781 catgagcaag ctctgttgct gaaggcgtac gtgaggcatt gtgacgaaga gcctcattca
      841 cgatatcctt caggcgctga gtcagcgcat ccctgtttgc ctttgctttc gcctgtgctt
      901 ccgctgcagc ttttgcccgg gcagcctgct ctgcctgtgt tttctttaat tgagcagtag
      961 accatttagc agttgcatga atagctgcag aactttcact tttactgcct ccttttccac
     1021 ctccgccgcc agagccactc ccgtcaggag taccattcaa aagagtaata attacctgtc
     1081 ccttatcatc ataaggaaca ccatctttat agtacgctac cgcggtttcc attataaaat
     1141 cctctttgac ttttaaaaca ataagttaaa aataaatact gtacatataa ccactggttt
     1201 tatatacagc ataaaagcta cgccgctgca ttttccctgt caagactgtg gacttccatt
     1261 tttgtgaaaa cgatcaaaaa aacagtcttt cacaccacgc gctattctcg cccgatgcca
     1321 caaaaaccag cacaaacatt accgttctca gacctcatta tgttttactg aaactatgag
     1381 atgagacatc tatgggacac tgtcacttta tggcatggca cacactccgg gacgcactaa
     1441 aaatgacagg cagatcgcgt tcacagtttt accgtgatat gcgcgcaggc cttgtcagtt
     1501 accgtaccgg cagggacgga cgacgggagt ttgaaaccag tgaactgatc cgggcatacg
     1561 gcgaattaaa gcagaatgag acaccagaaa ggcacagtga gggacatgca gaaaatccac
     1621 atgatcagca gacagaacgc attctccggg agctgaatga gctgaaacaa tgcctgacgc
     1681 tgatgcttga ggataaacag gcacaggata tggatcgcag acgccaggaa gcagaacggg
     1741 aacagctaca aaatgagata gcccagctca ggcaggcact ggaactggaa aagaaacggg
     1801 gattctggtc caggttgttc ggtcgctgaa cgctgtcaga gactgatgat aaaatagtct
     1861 tcggataata actcaccgag aataaatact ttaaggtagg gagacactca tgagacgtac
     1921 cggaaacaaa ctttgtctta tcgccatgat aacagcaaca gtagctctca cagcctgtac
     1981 cccaaagggc agcgtggaac aacatacccg gcattacgta tatgcttctg atgacggttt
     2041 tgatcccaac ttttccaccc aaaaagccga cacaacacga atgatggtgc ctttttttcg
     2101 gcagttctgg gatatgggag ctaaagataa agcgacagga aaatcacgga gtgatgtgca
     2161 acaacgcatt cagcagtttc acagccagga atttttaaac tcactccggg gcacaactca
     2221 atttgcgggt actgattacc gcagcaaaga ccttaccccg aaaaaatcca ggctgctggc
     2281 tgacacgatt tctgcggttt atctcgatgg ctacgagggc agacagtaag tggatttacc
     2341 ataatccctt aattgtacgc accgctaaaa cgcgttcagc gcgatcacgg cagcagacag
     2401 gtaaaaatgg caacaaacca cccgaaaaac tgccgcgatc gcgcctgata aattttaacc
     2461 gtatgaatac ctatgcaacc agagggtaca ggccacatta cccccactta atccactgaa
     2521 gctgccattt ttcatggttt caccatccca gcgaagggcc atccagcgtg cgttcctgta
     2581 tttccggctg acgctcccgt tccagggata acacatgttc gcgctcctgt atcagccgtt
     2641 cctctcttat ctccagttct cgctgtataa ctggctcaag cgttctgtct gctcgctcaa
     2701 gcgttctgtc tgctcgctca agtgctgcac ctgctgactc aactgcatga cccgctcgtt
     2761 cagcatcgcg ttgtcccgtt gcgtaagcga aaacatcttc tgcaattcca cgaaggcgct
     2821 ctcccattcg ctcagccgct gcatatagtc ctgttgcagc tgctctaagg cgttcagcaa
     2881 atgtgtttcc agctctgtca ctctgtgtca ctccttcaga tgtacccact ctttcccctg
     2941 aaagggaatc acctccgctg atttcccgta cggaaggaca aggaatttcc tgttcccgtc
     3001 ctgcacaaac tccacgcccc atgtcttcgc gttcagtttc tgcaatgtct cttcctgctt
     3061 cctgatttct tccaggttcg cctgtatcct ccctccaaga taccagagcg tcccgccact
     3121 cgcggtaaac aggagaaaga ctatccccag taacatcatg cccgtattcc ctgccagctt
     3181 taacacgtcc ctcctgtgct gcatcatcgc ctctttcacc ccttcccggt gtttttccag
     3241 cgattcctct gtcgaggctg tgaacagggc tatagcgtct ctgattttcg tctcgtttga
     3301 tgtcacagcc tcgcttacag attcgccgag cctcctgaac tcgttgttca gcattttctc
     3361 tgtagattcg gctctctctt tcagcttttt ctcgaactcc gcgcccgtct gcaaaagatt
     3421 gctcataaaa tgctcctttc agcctgatat tcttcccgcc gttcggatct gcaatgctga
     3481 tactgcttcg cgtcaccctg accacttcca gccccgcctc agtgagcgcc tgaatcacat
     3541 cctgacggcc ttttatctct ccggcatggt aaagtgcatc tatacctcgc gtgacgccct
     3601 cagcaagcgc ctgtttcgtt tcaggcaggt tatcagggag tgtcagcgtc ctgcggttct
     3661 ccggggcgtt cgggtcatgc agcccgtaat ggtgattcac cagcgtctgc caggcatcaa
     3721 ttctaggcct gtctgcgcgg tcgtagtacg gctggaggcg ttttccggtc tgtagctcca
     3781 tgttcggaat gacaaaattc agctcaagcc gtcccttgtc ctggtgctcc acccacagga
     3841 tgctgtactg atttttttcg agaccgggca tcagtacacg ctcaaagctc gccatcactt
     3901 tttcacgtcc tcccggcggc agctccttct ccgcgaacga cagaacaccg gacgtgtatt
     3961 tcttcgcaaa tggcgtggca tcgatgagtt cccggacttc ttccggatta ccctgaagca
     4021 ccgttgcgcc ttcgcggtta cgctccctcc ccagcaggta atcaaccgga ccactgccac
     4081 caccttttcc cctggcatga aatttaacta tcatcccgcg ccccctgttc cctgacggcc
     4141 agacgcagcc ggcgcagctc atccccgatg gccatcagtg cggccaccac ctgaacccgg
     4201 tcaccggaag accactgccc gctgttcacc ttcctcgctg tctgattcag gttattcccg
     4261 atggcggcca gctgacgcag taacggcggt gccagtgtcg gcagcttccc ggaacgggca
     4321 accggctcac ccaggcagac ccgccgcatc catactgcca gttgtttacc ctcacagcgt
     4381 tcaagtaacc tggcatgttc atcatcagta acccgtatcg tgagcatcct ctcgcgtttc
     4441 atcggtatca ttaccccatg aacagaaata cccccttaca cggaggcatc agtgactaaa
     4501 caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt aacgctgctg
     4561 gagaagctca acgaactgga cgcagatgaa caggcagaca tctgtgaatc gcttcacgac
     4621 cacgccgatg agctttaccg cagctgcctc gcacgtttcg gggatgacgg tgaaaacctc
     4681 tgacacatgc agctcccgga gacggtcaca gcttgtctgt gagcggatgc cgggagcaga
     4741 caagcccgtc agggcgcgtc agcaggtttt agcgggtgtc ggggcgcagc cctgacccag
     4801 tcacgtagcg atagcggagt gtatactggc ttaaccatgc ggcattagtg cggattgtat
     4861 gaaaagtacg ccatgccggg tgtgaaatgc cgcacagatg cgtaaggaga aaatgcacgt
     4921 ccaggcgctt ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc
     4981 gagcggtact gactcacaca aaaacggtaa cacagttatc cacagaatca ggggataagg
     5041 ccggaaagaa catgtgagca aaagaccaag aacaggaaga aggccacgta gcaggcgttt
     5101 ttccataggc tccgcccccc tgacgagcat cacaaaaata gacgctcaag tcagaggtgg
     5161 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5221 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5281 gtggcgcttt ctcatagctc acgctgttgg tatctcagtt cggtgtaggt cgttcgctcc
     5341 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5401 tatcgtcttg agtccaaccc ggtaaggcac gccttaacgc cactggcagc agccactggt
     5461 aaccggatta gcagagcgat gatggcacaa acggtgctac agagttcttg aagtagtggc
     5521 ccgactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     5581 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgtt ggtaacggtg
     5641 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     5701 tgatcttttc tactgaaccg cgatccccgt cagtttagaa gaggaggatg gtgcgatggt
     5761 ccctccctga acatcacgta tatagttagc ctgacatcca acaaggaggt ttatcgcgaa
     5821 tattcccaca aaaaatcttt tcctcataac tcgatcctta taaaatgaaa agaatatatg
     5881 gcgaggttta atttatgagc ttaagatact acataaaaaa tattttattt ggcctgtact
     5941 gcacacttat atatatatac cttataacaa aaaacagcga agggtattat ttccttgtgt
     6001 cagataagat gctatatgca atagtgataa gcactattct atgtccatat tcaaaatatg
     6061 ctattgaata catagctttt aacttcataa agaaagattt tttcgaaaga agaaaaaacc
     6121 taaataacgc ccccgtagca aaattaaacc tatttatgct atataatcta ctttgtttgg
     6181 tcctagcaat cccatttgga ttgctaggac tttttatatc aataaagaat aattaaatcc
     6241 ctaacacctc atttatagta ttaagtttat tcttatcaat ataggagcat agaattcctg
     6301 taacaatagc aataccccaa atacctaatg tagttccagc aagcaagcta aaaagtaaag
     6361 caacaacata actcacccct gcatctgcag ctttcttctc taatgtaaga aatagtggct
     6421 tccagtcacc tgtatcctta atttttagga tatcagatac cacatcatat ccaaaagaaa
     6481 catgccccgt aatcttcaag tacttggcaa actgatctaa atgtttagcc cagtcatcat
     6541 acttcaccga tgccaacgca ttaaaaatag catcacgatc ggctttgctg aatttcttat
     6601 ttaaaacatc cttgtatttt tcaaaagcag cgagagcttc attcacattg ccgattttct
     6661 tacctttag
//
