LOCUS       CP057736                6669 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB16-C13 plasmid pRHB16-C13_6, complete
            sequence.
ACCESSION   CP057736
VERSION     CP057736.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148137
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6669)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH02 T2-C13
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 102.558880900888x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 12:35:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,948
            CDSs (total)                      :: 5,829
            Genes (coding)                    :: 5,602
            CDSs (with protein)               :: 5,602
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 70 of 227
            Pseudo Genes (incomplete)         :: 134 of 227
            Pseudo Genes (internal stop)      :: 42 of 227
            Pseudo Genes (multiple problems)  :: 15 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB16-C13"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB16-C13_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(125..544)
                     /gene="exc2"
                     /locus_tag="HVX20_29665"
     CDS             complement(125..544)
                     /gene="exc2"
                     /locus_tag="HVX20_29665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMM14545.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(625..1062)
                     /locus_tag="HVX20_29670"
     CDS             complement(625..1062)
                     /locus_tag="HVX20_29670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QMM14546.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            1322..2890
                     /gene="cea"
                     /locus_tag="HVX20_29675"
     CDS             1322..2890
                     /gene="cea"
                     /locus_tag="HVX20_29675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="QMM14547.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(2887..3228)
                     /locus_tag="HVX20_29680"
     CDS             complement(2887..3228)
                     /locus_tag="HVX20_29680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="QMM14548.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            3276..3413
                     /locus_tag="HVX20_29685"
     CDS             3276..3413
                     /locus_tag="HVX20_29685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QMM14549.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(3612..3770)
                     /locus_tag="HVX20_29690"
     CDS             complement(3612..3770)
                     /locus_tag="HVX20_29690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QMM14550.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(4440..4631)
                     /locus_tag="HVX20_29695"
     CDS             complement(4440..4631)
                     /locus_tag="HVX20_29695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMM14551.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4696..5019
                     /locus_tag="HVX20_29700"
     CDS             4696..5019
                     /locus_tag="HVX20_29700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMM14554.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4961..5719
                     /locus_tag="HVX20_29705"
                     /pseudo=""
     CDS             4961..5719
                     /locus_tag="HVX20_29705"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5697..6215
                     /locus_tag="HVX20_29710"
     CDS             5697..6215
                     /locus_tag="HVX20_29710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbeB"
                     /protein_id="QMM14552.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="mobilization protein MbeB"
     gene            6222..6476
                     /gene="mbeD"
                     /locus_tag="HVX20_29715"
     CDS             6222..6476
                     /gene="mbeD"
                     /locus_tag="HVX20_29715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMM14553.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSEQTERLSQLYSENWR"
                     /name="mbeD"
     Typing          4268..4355
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          complement(3699..4254)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.52"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5010..6580
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.809"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atttatcagg cgcgatcgcg gcagtttttc gggtggtttg ttgccatttt tacctgtctg
       61 ctgccgtgat cgcgctgaac gcgttttagc ggtgcgtaca attaagggat tatggtaaat
      121 ccacttactg tctgccctcg tagccatcga gataaaccgc agaaatcgtg tcagccagca
      181 gcctggattt tttcggggta aggtctttgc tgcggtaatc agtacccgca aattgagttg
      241 tgccccggag tgagtttaaa aattcctggc tgtgaaactg ctgaatgcgt tgttgcacat
      301 cactccgtga ttttcctgtc gctttatctt tagctcccat atcccagaac tgccgaaaaa
      361 aaggcaccat cattcgtgtt gtgtcggctt tttgggtgga aaagttggga tcaaaaccgt
      421 catcagaagc atatacgtaa tgccgggtat gttgttccac gctgcccttt ggggtacagg
      481 ctgtgagagc tactgttgct gttatcatgg cgataagaca aagtttgttt ccggtacgtc
      541 tcatgagtgt ctccctacct taaagtattt attctcggtg agttattatc cgaagactat
      601 tttatcatca gtctctgaca gcgttcagcg accgaacaac ctggaccaga atccccgttt
      661 cttttccagt tccagtgcct gcctgagctg ggctatctca ttttgtagct gttcccgttc
      721 tgcttcctgg cgtctgcgat ccatatcctg tgcctgttta tcctcaagca tcagcgtcag
      781 gcattgtttc agctcattca gctcccggag aatgcgttct gtctgctgat catgtggatt
      841 ttctgcatgt ccctcactgt gcctttctgg tgtctcattc tgctttaatt cgccgtatgc
      901 ccggatcagt tcactggttt caaactcccg tcgtccgtcc ctgccggtac ggtaactgac
      961 aaggcctgcg cgcatatcac ggtaaaactg tgaacgcgat ctgcctgtca tttttagtgc
     1021 gtcccggagt gtgtgccatg ccataaagtg acagtgtccc atagatgtct catctcatag
     1081 tttcagtaaa acataatgag gtctgagaac ggtaatgttt gtgctggttt ttgtggcatc
     1141 gggcgagaat agcgcgtggt gtgaaagact gtttttttga tcgttttcac aaaaatggaa
     1201 gtccacagtc ttgacaggga aaatgcagcg gcgtagcttt tatgctgtat ataaaaccag
     1261 tggttatatg tacagtattt atttttaact tattgtttta aaagtcaaag aggattttat
     1321 aatggaaacc gcggtagcgt actataaaga tggtgttcct tatgatgata agggacaggt
     1381 aattattact cttttgaatg gtactcctga cgggagtggc tctggcggcg gaggtggaaa
     1441 aggaggcagt aaaagtgaaa gttctgcagc tattcatgca actgctaaat ggtctactgc
     1501 tcaattaaag aaaacacagg cagagcaggc tgcccgggca aaagctgcag cggaagcaca
     1561 ggcgaaagca aaggcaaaca gggatgcgct gactcagcgc ctgaaggata tcgtgaatga
     1621 ggctcttcgt cacaatgcct cacgtacgcc ttcagcaaca gagcttgctc atgctaataa
     1681 tgcagctatg caggcggaag cagagcgttt gcgccttgcg aaagcagaag aaaaagcccg
     1741 taaagaagcg gaagcagcag aaaaggcttt tcaggaagca gaacaacgac gtaaagagat
     1801 tgaacgggag aaggctgaaa cagaacgcca gttgaaactg gctgaagctg aagagaaacg
     1861 actggctgca ttgagtgaag aagctaaagc tgttgagatc gcccaaaaaa aactttctgc
     1921 tgcacaatct gaagtggtga aaatggatgg agagattaag actctcaatt ctcgtttaag
     1981 ctccagtatc catgcccgtg atgcagaaat gaaaacgctc gctggaaaac gaaatgaact
     2041 ggctcaggca tccgctaaat ataaagaact ggatgagctg gtcaaaaaac tatcaccaag
     2101 agccaatgat ccgcttcaga accgtccttt ttttgaagca accagacgac gggttggggc
     2161 cggtaagatt agagaagaaa aacaaaaaca ggtaacagca tcagaaacac gtattaaccg
     2221 gataaatgct gatataactc agatccagaa ggctatttct caggtcagta ataatcgtaa
     2281 tgccggtatc gctcgtgttc atgaagctga agaaaatttg aaaaaagcac agaataatct
     2341 ccttaattca cagattaagg atgctgttga tgcaacagtt agcttttatc aaacgctgac
     2401 tgaaaaatat ggtgaaaaat attcgaaaat ggcacaggaa cttgctgata agtctaaagg
     2461 taagaaaatc ggcaatgtga atgaagctct cgctgctttt gaaaaataca aggatgtttt
     2521 aaataagaaa ttcagcaaag ccgatcgtga tgctattttt aatgcgttgg catcggtgaa
     2581 gtatgatgac tgggctaaac atttagatca gtttgccaag tacttgaaga ttacggggca
     2641 tgtttctttt ggatatgatg tggtatctga tatcctaaaa attaaggata caggtgactg
     2701 gaagccacta tttcttacat tagagaagaa agctgcagat gcaggggtga gttatgttgt
     2761 tgctttactt tttagcttgc ttgctggaac tacattaggt atttggggta ttgctattgt
     2821 tacaggaatt ctatgctcct atattgataa gaataaactt aatactataa atgaggtgtt
     2881 agggatttaa ttattcttta ttgatataaa aagtcctagc aatccaaatg ggattgctag
     2941 gaccaaacaa agtagattat atagcataaa taggtttaat tttgctacgg gggcgttatt
     3001 taggtttttt cttctttcga aaaaatcttt ctttatgaag ttaaaagcta tgtattcaat
     3061 agcatatttt gaatatggac atagaatagt gcttatcact attgcatata gcatcttatc
     3121 tgacacaagg aaataatacc cttcgctgtt ttttgttata aggtatatat atataagtgt
     3181 gcagtacagg ccaaataaaa tattttttat gtagtatctt aagctcataa attaaacctc
     3241 gccatatatt cttttcattt tataaggatc gagttatgag gaaaagattt tttgtgggaa
     3301 tattcgcgat aaacctcctt gttggatgtc aggctaacta tatacgtgat gttcagggag
     3361 ggaccatcgc accatcctcc tcttctaaac tgacggggat cgcggttcag tagaaaagat
     3421 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3481 accaccgtta ccaacggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3541 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtc
     3601 gggccactac ttcaagaact ctgtagcacc gtttgtgcca tcatcgctct gctaatccgg
     3661 ttaccagtgg ctgctgccag tggcgttaag gcgtgcctta ccgggttgga ctcaagacga
     3721 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3781 ttggagcgaa cgacctacac cgaactgaga taccaacagc gtgagctatg agaaagcgcc
     3841 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3901 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3961 cgccacctct gacttgagcg tctatttttg tgatgctcgt caggggggcg gagcctatgg
     4021 aaaaacgcct gctacgtggc cttcttcctg ttcttggtct tttgctcaca tgttctttcc
     4081 ggccttatcc cctgattctg tggataactg tgttaccgtt tttgtgtgag tcagtaccgc
     4141 tcgccgcagt cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg aaaagcgcct
     4201 ggacgtgcat tttctcctta cgcatctgtg cggcatttca cacccggcat ggcgtacttt
     4261 tcatacaatc cgcactaatg ccgcatggtt aagccagtat acactccgct atcgctacgt
     4321 gactgggtca gggctgcgcc ccgacacccg ctaaaacctg ctgacgcgcc ctgacgggct
     4381 tgtctgctcc cggcatccgc tcacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     4441 cagaggtttt caccgtcatc cccgaaacgt gcgaggcagc tgcggtaaag ctcatcggcg
     4501 tggtcgtgaa gcgattcaca gatgtctgcc tgttcatctg cgtccagttc gttgagcttc
     4561 tccagcagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc
     4621 tgtttagtca ctgatgcctc cgtgtaaggg ggtatttctg ttcatggggt aatgataccg
     4681 atgaaacgcg agaggatgct cacgatacgg gttactgatg atgaacatgc caggttactt
     4741 gaacgctgtg agggtaaaca actggcagta tggatgcggc gggtctgcct gggtgagccg
     4801 gttgcccgtt ccgggaagct gccgacactg gcaccgccgt tactgcgtca gctggccgcc
     4861 atcgggaata acctgaatca gacagcgagg aaggtgaaca gcgggcagtg gtcttccggt
     4921 gaccgggttc aggtggtggc cgcactgatg gccatcgggg atgagctgcg ccggctgcgt
     4981 ctggccgtca gggaacaggg ggcgcgggat gatagttaaa tttcatgcca ggggaaaagg
     5041 tggtggcagt ggtccggttg attacctgct ggggagggag cgtaaccgcg aaggcgcaac
     5101 ggtgcttcag ggtaatccgg aagaagtccg ggaactcatc gatgccacgc catttgcgaa
     5161 gaaatacacg tccggtgttc tgtcgttcgc ggagaaggag ctgccgccgg gaggacgtga
     5221 aaaagtgatg gcgagctttg agcgtgtact gatgcccggt ctcgaaaaaa atcagtacag
     5281 catcctgtgg gtggagcacc aggacaaggg acggcttgag ctgaattttg tcattccgaa
     5341 catggagcta cagaccggaa aacgcctcca gccgtactac gaccgcgcag acaggcctag
     5401 aattgatgcc tggcagacgc tggtgaatca ccattacggg ctgcatgacc cgaacgcccc
     5461 ggagaaccgc aggacgctga cactccctga taacctgcct gaaacgaaac aggcgcttgc
     5521 tgagggcgtc acgcgaggta tagatgcact ttaccatgcc ggagagataa aaggccgtca
     5581 ggatgtgatt caggcgctca ctgaggcggg gctggaagtg gtcagggtga cgcgaagcag
     5641 tatcagcatt gcagatccga acggcgggaa gaatatcagg ctgaaaggag cattttatga
     5701 gcaatctttt gcagacgggc gcggagttcg agaaaaagct gaaagagaga gccgaatcta
     5761 cagagaaaat gctgaacaac gagttcagga ggctcggcga atctgtaagc gaggctgtga
     5821 catcaaacga gacgaaaatc agagacgcta tagccctgtt cacagcctcg acagaggaat
     5881 cgctggaaaa acaccgggaa ggggtgaaag aggcgatgat gcagcacagg agggacgtgt
     5941 taaagctggc agggaatacg ggcatgatgt tactggggat agtctttctc ctgtttaccg
     6001 cgagtggcgg gacgctctgg tatcttggag ggaggataca ggcgaacctg gaagaaatca
     6061 ggaagcagga agagacattg cagaaactga acgcgaagac atggggcgtg gagtttgtgc
     6121 aggacgggaa caggaaattc cttgtccttc cgtacgggaa atcagcggag gtgattccct
     6181 ttcaggggaa agagtgggta catctgaagg agtgacacag agtgacagag ctggaaacac
     6241 atttgctgaa cgccttagag cagctgcaac aggactatat gcagcggctg agcgaatggg
     6301 agagcgcctt cgtggaattg cagaagatgt tttcgcttac gcaacgggac aacgcgatgc
     6361 tgaacgagcg ggtcatgcag ttgagtcagc aggtgcagca cttgagcgag cagacagaac
     6421 gcttgagcga gcagacagaa cgcttgagcc agttatacag cgagaactgg agataagaga
     6481 ggaacggctg atacaggagc gcgaacatgt gttatccctg gaacgggagc gtcagccgga
     6541 aatacaggaa cgcacgctgg atggcccttc gctgggatgg tgaaaccatg aaaaatggca
     6601 gcttcagtgg attaagtggg ggtaatgtgg cctgtaccct ctggttgcat aggtattcat
     6661 acggttaaa
//
