LOCUS       CP057719                6988 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB17-C01 plasmid pRHB17-C01_3, complete
            sequence.
ACCESSION   CP057719
VERSION     CP057719.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148141
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6988)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH03 T2-C01
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 124.846954719754x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 11:09:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,670
            CDSs (total)                      :: 4,551
            Genes (coding)                    :: 4,312
            CDSs (with protein)               :: 4,312
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 124 of 239
            Pseudo Genes (incomplete)         :: 93 of 239
            Pseudo Genes (internal stop)      :: 41 of 239
            Pseudo Genes (multiple problems)  :: 17 of 239
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB17-C01"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB17-C01_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(5343..6988,1)
                     /gene="cka"
                     /locus_tag="HVX24_23280"
     CDS             join(5343..6988,1)
                     /gene="cka"
                     /locus_tag="HVX24_23280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="QML94492.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     gene            complement(join(6986..6988,1..288))
                     /gene="cki"
                     /locus_tag="HVX24_23285"
     CDS             complement(join(6986..6988,1..288))
                     /gene="cki"
                     /locus_tag="HVX24_23285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="QML94483.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            318..449
                     /locus_tag="HVX24_23290"
     CDS             318..449
                     /locus_tag="HVX24_23290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QML94484.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(1455..1646)
                     /locus_tag="HVX24_23295"
     CDS             complement(1455..1646)
                     /locus_tag="HVX24_23295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QML94485.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1714..2037
                     /locus_tag="HVX24_23300"
     CDS             1714..2037
                     /locus_tag="HVX24_23300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QML94486.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2000..2743
                     /locus_tag="HVX24_23305"
                     /pseudo=""
     CDS             2000..2743
                     /locus_tag="HVX24_23305"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2715..3275
                     /locus_tag="HVX24_23310"
     CDS             2715..3275
                     /locus_tag="HVX24_23310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QML94487.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            3282..3515
                     /gene="mbeD"
                     /locus_tag="HVX24_23315"
     CDS             3282..3515
                     /gene="mbeD"
                     /locus_tag="HVX24_23315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QML94488.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="mbeD"
     gene            complement(3999..4421)
                     /gene="exc2"
                     /locus_tag="HVX24_23320"
     CDS             complement(3999..4421)
                     /gene="exc2"
                     /locus_tag="HVX24_23320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QML94489.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTA
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            4543..4821
                     /locus_tag="HVX24_23325"
     CDS             4543..4821
                     /locus_tag="HVX24_23325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QML94490.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(4813..5103)
                     /locus_tag="HVX24_23330"
     CDS             complement(4813..5103)
                     /locus_tag="HVX24_23330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QML94491.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     Typing          1283..1370
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          complement(716..1272)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.633"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2028..3622
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.624"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atttctcttt agagtgtaat atatgaaact tggcaatcct actgggattg ccattaataa
       61 ataaaacaat gcagataact tgcataagta ccagggttct ttttttaact ttgtattttc
      121 tttgatatat ctagatatgg ttaatttaga gtatggatat agcaatacat gtattgatga
      181 tataaatata aataacaaag gagtgttatc attgtcgtta aatattaaaa taagaatcca
      241 tggtataagt gattcaggta aattatgtag gtagtatttt aaatgcataa gtatttttac
      301 ttttgttgga aagaaatatg aaaataataa tttttctaat tacaatatta ttcttgtcag
      361 gatgtcaagc aaactatatt cgtgatgtac aaggaggaac cgttgcacca tcttcgtctt
      421 ctgaactgac ggggatcgcg gttcagtaga aaagatcaaa ggatcttctt gagatccttt
      481 ttttctgcgc gtaatctgct gcctgtaaac gaaaaaaccg cctggggagg cggtttagtc
      541 gaaggttaag tcagttgggg actgcttaac cgggtaactg gctttagtgg agcacagata
      601 ccaaatgctg tccttccagt gtagccgcta taaggcttca acttcaagag ttctgcgtat
      661 ctaaatccac tatccagtta ccaatggctg ctgccagtgg cgttaagtcg tgtcttaccg
      721 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
      781 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac caacagcgtg
      841 agctatgaga aagcgccacg cttcccaaag ggagaaaggc ggacaggtat ccggtaagcg
      901 gcagggtcgg aacaggagag cgcacgaggg agccaccagg gggaaacgcc tggtatcttt
      961 aagtcctgtc gggtttcgcc acctctgact tgagcgtcgg tttttgtgat gctcgtcagg
     1021 ggggcggagc ctatggaaaa acgccagctg cgtggccttt tccgggttct tgttttttgc
     1081 tcacatgttt cttttggtgt tatcccctga ttctgtggat aaccgcatta ccgtctttga
     1141 gtgagctgat accgctcgcc gcagccgaac gaccgagcgg agcgagtcag tgagcgagga
     1201 agcggaaaag cgcctggacg agcattttct ccttacgcat ctgtgcggca tttcacaccc
     1261 ggcatggtgc acttttcata caatcctcat tgatgccaaa tgattaagcc agtatacact
     1321 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgctaaa acccgctgac
     1381 gcgccctgac gggcttgtct gctcccggca tccgctcaca ggtcagctgt gaccgtctcc
     1441 gggagctgca tgtgtcagag gttttcaccg tcatccccga agcgtgcgag gcagctgcgg
     1501 taaagctcgt cagcgtggtc gtgaagcgct tcgcagatgt cagcctggtc gtcggcgtcc
     1561 agttcattca gtttttccag cagggtcagc gtctggcttc tgatgaagcg ggccatgtta
     1621 agggcggttt tttcctgttt agtcacagtt ctgtttctcc atgtgtgggg agtctgtcgg
     1681 aggtttttaa tgataccggt gaaacgcgaa aggatgctca cgatacgggt cacggatgag
     1741 gaacatgcgc ggctgctggc acgttgtgag ggaaaacaac ttgcgtcatg gatgcgtaag
     1801 gtctgcctgg gggcaccgcc atcgaaaacg tcaggtcttc cgacactggc accgccgctg
     1861 ttgcgccagt tcgcttcggt cggcaataac ctgaatcaga tagcccggaa gataaacagc
     1921 ggacagtggt cggggcatga ccgggttcat gtggtggctg cactgatggc catcggacgt
     1981 gaactgagtg agctgaggga tgaggtccgt aaacagggag aacgggatga tagttaagtt
     2041 tcatgccagg ggaaaaggcg gtggcagtgg tccggttgat tacctgctgg gccgggagcg
     2101 taaccgggaa ggggcgagag ttctgcgggg tgctcccgaa gaggtgcggg aactcatcga
     2161 tgccacgccg tttgcgaaaa aatacacctc gggggttctg tcttttgcag agcagaccct
     2221 gccaccggga gagcgtgaaa gggtgatgga gagctttgag cgggtactga tgcccggtct
     2281 tgaaaaaaat cagtacagca tcctgtgggt ggagcaccag gacaaggggc gtcttgagct
     2341 gaattttgtc atcccgaata tggagctggc gagcggaaaa cggctccagc cctattacga
     2401 ccgagcagac cgtccgcgaa tcaatgcctg gcagacactg gtgaatcacc attacgggct
     2461 gcacgacccg aacgcaccgg agaacaggcg gacacttgtc accccgaata atctgccgaa
     2521 agcgaaacag gaggccgcag aagcgattac gaggggactg gaggctcttt accatgccgg
     2581 agcgctaaaa acgcgtcagg atgtcactga ggcgcttaca gcggcaggtt ttgaggttgt
     2641 gaggacaacc aggagcagta tcagtatcgc cgacccggag ggtgggcgta atctcaggct
     2701 gaaaggggcg ctgtatgagc aatcttttac aaatggggac agacttcgag aagaaactga
     2761 aagagcgtgc cgtctctaca gagaacatgc tgaacagcga gttcaggaag ctcgaagcgt
     2821 ctgtagacag ggaactgaac ttaaacgggc agaaaatcag agacgctatc agcgcacaca
     2881 cagcagcggt gaaagaacaa ctggagacgc tgaacagtgc cgtgaacgcg cagttcagcg
     2941 ccacagagaa cgaactggcc cggcggcagg aagagctgct ctggaagctg gtcaaagggc
     3001 ggatactgta cccgtcgctg acggcgcttt gcgtcacggg cgggattttt ctggcgtcat
     3061 gggggctgat tcagtggcag gaaagccgga ttgcggcgaa catcctggct atcagggacc
     3121 aggaagagac gctggcgaaa ctccgggaaa aaacatgggg tgtgacgtat cacgagggca
     3181 ggaacgggaa atttctggtg ttgccgtccg gggtgaaggg ggaaaacaac tggacggtgg
     3241 agaagaagaa cgcagtaaga ctggtcaggg agtaagtgag tatgacagag ctggaaatgc
     3301 acttactgaa cgccttagag caattgcagc aggactatac gcagcggctg aaagactggg
     3361 agagcgcctt cacggatttg cagcatatgt tcgagctgac acgacaggag aacgggcaac
     3421 tgagggaaca gtgcgacgac ttgagtcagc aggtgcagag attagccggg caggtggacc
     3481 acttgagtcg gttatttatc acgaacagca gataagggat gaacaggaaa ggcaggcacg
     3541 cctggaacgg gagcgtgcgc ggtcctggca tgaagagcag gaacgggaag cacgggtatg
     3601 gcgtggcccg tcaataggga tgtgacggtg agttgttccg ggaggttgcc agatggtacc
     3661 ggaacgcgtt aaaaacgcat ccgggcgcga ttctggcgtg tcgtttttgt tgctgtgttt
     3721 tgcctctttg ccagatcgcg ctcattcctc agcctcatcc cgcgatgtct gcgtatgtca
     3781 tcaactgaaa agatggaaaa aaaacagtgt catgaggttt acgcaggcaa aactagcgtt
     3841 attcacatgg ctgaatagct atgcatacgg ttaaaattta tcaggtgcga tcgcggcagc
     3901 ttttcgggtg gtttgttgcc atttttacct gtcagcagcc gtgatcgcgc tgaacgcatt
     3961 tcagcggtgc gtacaattaa gggattatgg taaatccctt actgtctgcc ctcgtatcca
     4021 tcgagataaa ccgcagaaat ggtgtctgcc attagtttca tttttttcgg cgaaggcata
     4081 tcgctgtttg tgtattttct gccagcaaag gctgttgtgc tccggagtga gtgaaaaaat
     4141 ttttctgagt ggaattcttt tacacgttgc tgtgccgttt ctgctgatac tccggctgct
     4201 ttgtctttca tccccatatc ccggaactgc tgaaagaacg ggagcatcat acgtatggtg
     4261 tctgctttct gggtgtagaa gttaggatca aatccatcat ctgaagcata aacataatgc
     4321 cgggtatgtc gttccaggct ggtctttggt gtgcatcctg taagagctat tgttgctgtt
     4381 atcagaggta tcagaagcag cctggcagcg gcagatctca taaacatctc cttgcgtttt
     4441 taatcttgat actcagggag ttatatctcc gaagagtatc tcttatcata cgtcggttta
     4501 ctcccaatgt gatttgatgg tcaatgagtc aggaataatc ccgtgaaaaa atgtgtgcca
     4561 gtcctgcttt ttagtgttct ttctgcttcg gttgccagtc atgcggacag caatgttttt
     4621 caacaaaagg ccgagaagac aaaaaacggc attgttgcgg atgttaatgc cagacagaaa
     4681 atgttcagta acagactgaa gatacaactt cagatcgatg agttgagagc agagctggct
     4741 ggcaccagag atgcccaaaa aagagaggat ttgcgtaaaa aactggagtc tctggagcgg
     4801 caaaagaaat ccttacgctg atgggggcgt attttccccg gtttcttcca taaccttttt
     4861 accgttcaga tatttacggg cgaggcgaat gcgtcttgcc tggtataccg ccgagtaagc
     4921 ctgggtggga ttcacaccat tactgatttc actgaccaca tgacgctgat cagccagtgt
     4981 cagcgtgtaa acctgtttct ggatgaggtc acgggctttg tcatgctcat atccggcagc
     5041 aacaagggtg tcgagaagtc gtttgtctgt tttgttcagt ttatggcggg gtgatttgct
     5101 cacatcgttc tccgtttttt catgatccaa cagttttata tctcatcctg atttgctccg
     5161 gtgttctgtt tcgacgttct caatgcctgc ctccccatag tgtgatcctt attgcaaaaa
     5221 tgaaaatcca tgctcttgac atggacaatg ctgagtagta ggttttactg tatataaaac
     5281 cagtggttat atatacagta tattgttttt aatttattgt tttaattatc aaagaggaat
     5341 ttatggctaa agaactaagt ggatatggac caactgctgg tgagtctatg gggggaacag
     5401 gggcgaatct gaatcagcaa ggtggaaata ataatagtaa tagtggggtt cactggggtg
     5461 gtggctctgg gcatggtaat aatggtggtc agggaaattc aaattcatct ggttctacat
     5521 caactgtgat gaaaaccggt gagtcatatc tgactccgtg gggtgatgtt gtcattaata
     5581 atgatggcct tccggtgatg aatggtattg ttatgacaga agagaattca actctggttg
     5641 ataatccatt tggaggggtc tccagagttc ttaattcttt aattagtgat atgccatcac
     5701 tttttgcaga aagctcagga aataacaaca ataacactgc ttcagttaat actgcaccaa
     5761 cgaatgctca ggtcagtgat atggataaga gttcgaaggt ggtgggtaat gtaattaatg
     5821 aaaaacaaaa gcagaagaat aaaattgcta cacagattag tgaaaaacag aagaaaatcg
     5881 aggagatgaa aaaagtattc aagcatcatt cttatcatgg tatcactgat cttgagaggg
     5941 atgttgatga gttgcaaaaa aaatcaaatc agcttgatgc agatattagc aaacttaatt
     6001 cttataaaaa tacattgcaa agtaaaattg gtgatgtgaa taaacagaag gaagctgaag
     6061 aaaaggctcg tgaaaatgca gaggtcgctg aacatgaaac tcttaatgaa gagaaacagg
     6121 cggtggcgga agcagagaag cgtttagctg aggcgaaagc tgaactggcg aaggctgaaa
     6181 gtgatgtaca gagtaagcaa gctactgttt ccagagttgc aggagagctt gaaaacgctc
     6241 agaagagtgt tgacgtgaag gtcaccggat ttccgggatg gcgtgacgtt cagaaaaaac
     6301 tacagcgtca gcttgaagct aagcaggctg agtactccgc agttgaaaat gaacttaaga
     6361 atgctgttag tttcagggat ggtaaggccg ctgaggttaa agaggcagag cagaaattaa
     6421 aagaagctca ggatgctctt gaaaagagcc agataaaaga tgctgttgat actatggttg
     6481 gattttatca atatataacc gaacaatatg gagaaaaata cgcaaaaatt gctcaggatt
     6541 tagcggaaaa gtctaaagga aagaaaattc aaggtgttga tgaagctctg gcagcatttg
     6601 aaaagtataa gaatgtactg gataagaaat tcagtaaggt tgatcgggat gctattttta
     6661 atgcgttaga gtctgttaat tatgatgagt tatctaagaa tttaacgaag atatcaaagt
     6721 cgcttaaaat caccagtcga gtatcttttc tatatgatgt tggttctgat tttaaaaatg
     6781 caatagaaac tggtaactgg cgacctttgt ttgtcacgct ggagaaaagt gcggtcgatg
     6841 taggtgtggc aaaaattgtg gcattaatgt ttagttttat tgttggtgtg cctcttggtt
     6901 tctgggggat tgcaattgtc acaggtattg tttcttctta cataggggat gatgagttga
     6961 gcaaacttaa tgagttgtta gggattta
//
