LOCUS       CP057711                4072 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB17-C03 plasmid pRHB17-C03_7, complete
            sequence.
ACCESSION   CP057711
VERSION     CP057711.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148143
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH03 T2-C03
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 76.2135419097413x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 11:06:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,795
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,437
            CDSs (with protein)               :: 4,437
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 124 of 239
            Pseudo Genes (incomplete)         :: 93 of 239
            Pseudo Genes (internal stop)      :: 41 of 239
            Pseudo Genes (multiple problems)  :: 17 of 239
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB17-C03"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB17-C03_7"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(3456..4072,1..76))
                     /locus_tag="HVX26_23960"
     CDS             complement(join(3456..4072,1..76))
                     /locus_tag="HVX26_23960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QML85501.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(608..862)
                     /locus_tag="HVX26_23965"
     CDS             complement(608..862)
                     /locus_tag="HVX26_23965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QML85499.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobilization protein"
     gene            1041..3200
                     /locus_tag="HVX26_23970"
     CDS             1041..3200
                     /locus_tag="HVX26_23970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QML85500.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     Typing          complement(864..1040)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.438"
                     /coverage="100.0"
                     /evalue="2.610000000000001e-86"
     Typing          1041..1399
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          complement(3457..4072)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.117"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          955..3197
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.332"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aacgttgttc ctgtagggtc aacttgtagc tggcctcaac caaagcattc gacttgtagg
       61 caatcaagtc cgacatacaa agatcccgtt caaatgtggt gatgctttaa tgtgccccac
      121 aaataagaaa tgtggaatat ttttttttat atcctcacat ttctgtgaat aacaccttaa
      181 attcctccac ctttacctta aattcctcca cctaaacacc ttaaattcct ccacctttac
      241 cttaaattcc tccacatata cctctataac atattgattt tattaatgaa ttttgaccct
      301 aaaacaagta ttaaaaacaa gatcttaaaa acaagatcta aaaacttaac ggcgagcctg
      361 tggataactc ggggcgctga aaacaaaaaa ccaataaaaa caatgtgttg caagaaagca
      421 gaattgcaag ttccgaatac tccccttcca accctgtgga aatccagtat tcatgcgggt
      481 tcagcgcata tcacctaccg aaaaaccgtg cgtatgtgct caacctggcg acggattcac
      541 ttccgcttcc tcgcccactc gctcgctaac gctcggtcgc tggggctgcg gcgagcggac
      601 gatacaatca ctgtcctggc agcgcctcca gctcgttaat cagcggcagt atgcgcgtca
      661 catcagcctg tcgtgtcacc tctgccttca ggaactggag taaccaggca cgcaactgca
      721 cctcctccgg ctttctgacc tttgccagca atgctgcacc cacaacaacc ttctgcgctg
      781 tgtccaggct ccggctcttc gccttcaggc gctgtaatct cgcctcagca tcagcaatct
      841 tctgttcgag cgttctgctc atgatgtaac tccgtgcgcg atgaaaaatc gcattttagc
      901 gcgtcactgg tagtttaaaa actgagctgg cataatgcac gacaaatcac aaagtgcgca
      961 cttatacaat ctccactccg tttcgattgt gtgcgccctg cggggctgaa agaaaactac
     1021 aaaaaggcat tacggcagaa atggcgattt atcatctcag catgaaaatt atctccagaa
     1081 gcagcggcta cagcgccgta gcttccgctg cctatcgttc cggttcactg atgcttgatg
     1141 agcgtaccgg actgactcac gactacaccc gcaaaagcgg tgtggctgaa gctgtgattc
     1201 tgacccccgc gacagcccct gcctggtgta cgaaccgtgc cgaactctgg aatgccgttg
     1261 agaaggccga atgccggaag aactcacaac ttgcgcggga aattgaactc gctatccccc
     1321 gtgaactgcc gcaggatgcc gcacgggaaa ccgttctcgc ctttgtcaga gaaaactttg
     1381 tcagtcaggg catgattgct gatgtggcgt tccatcacat ggataaaaca aatccccatg
     1441 cgcacatcat gctgaccacg agggctgtcg gacctgctgg gttcggcgga aaggtcaggg
     1501 actggaacga ccggacacat gccgagacgt ggcgtgcatc atgggctgac catgcaaaca
     1561 gagcactggc gaacgccggt tatcaggaag agatagacca tcgttcatac gagcgacagg
     1621 ggcttgagaa aacgcctggt attcacctcg gaaagtctgc ctgtgcgatg gaaacacgcg
     1681 gtatcgaaac agaacgcggc gaacagaatc gtctgattaa caggcttaac ctggaaatac
     1741 agatttcccg cactgaactc aggaacagcg ggctgacaga gcctgcccgc cgtgtggctg
     1801 accttctgaa tatcgtgctt cctgacaacg caacttcgta cacactacgt gacatcatcg
     1861 aagcgttgcc gaaggacagt aatgcggcat ggaaactcac cagtaatgcc atgtcgatga
     1921 tggcagatat gcaggcgacg cgcagacgct gggaagagct gaacaccgaa cgaaaggaag
     1981 cgatgagcca tgctgccagc ctgaaaaaat catcgccgtt ttcgtccggt ttttcccgta
     2041 ttcccctgat gcaatgggcg gcaccggaat accgtagcga acagggaaaa atccagtcgc
     2101 tgagccagca aatggaaaaa ttgcgacagc attaccggaa ggtggagaaa caggacattc
     2161 ccgcgagtca gacggcattt gaaaaacagt ggcagcagtg gggggcatcc ggccttgcag
     2221 ctctcagagt ccagttgctt aaacgtgaag aggaattgcg ccggaaggag caggaggata
     2281 cagaacgtag tcgcgcggag cgacgagcgg aacagctcag gaaaaatgac aatgccgtta
     2341 tggattcagg ggtactgagc gcagtcgtta cggggtatgg cgaggccccc atgcctggta
     2401 acggggaaat gacctgttac cttctgcttc acaaccggaa cggagaatac acgctctggg
     2461 ggaacgagct ggagaaatac cgcaccagaa tctttgagcg tgttgattta atgcgtgaca
     2521 ggagtggtta tatctgtgac cgaagcgaaa tcggtcaaca tccaccactg caacgtgttt
     2581 attcctctgc gacctttgag caacttctga cgcaggtttg ccagacatgg ccgcaatata
     2641 cccgcaatct ccgccagccg aagacatggc cggagtcttt ctgtcttgga gaagaccgcc
     2701 agccagccat gccatcactg gcagcaagga aggtggattt tacgcaggga cgtctgctac
     2761 cgacgctgat gccggtaatg agttctgtcg accgggaaac ccgccagctt cagcttttgc
     2821 tggtcatggg cgtggatgac tctctgggcg gtgttgtccg gcttaatggt acgctgtatc
     2881 ccgcgtttgc cgttccctca gccgataaca gccagttggt catcagtgca ctgaccgata
     2941 aggggttacg gtatgcaggg tatggtgttg ccgtaaatca cgatgcagac agccacatat
     3001 cacctgcgcc ggaacttatg gagtttcatc tgaaaacccg tgaggcaccg ctttttgctg
     3061 ccgtcaacac accagaaaaa cagccggatc atctgttccg ctctctgggt ttcaatcgta
     3121 cctgggatga atggcggcgc gaggaagatg caagaacaca caccaccgag aggagacatg
     3181 atcgcggttg gtcacagtga atgtgaacaa tattttgttt tgtttaacat cgtgttatgt
     3241 tcgtcgatgt tcttctatgt tcatcgacag ggatgaatgc aagaagcaca cacataatgc
     3301 aagcatagtt ttgtttttcc gcccccaaaa attgggggtt cttttttcgc tgcattagag
     3361 cgcttttgac gcaatttttc acttcaatag taccttcgca ctactaacac aaaaaaaacg
     3421 cagcagggag cgttttaggt gccttaaaat cgattttaga tcgtcatctt gatctgtttg
     3481 tcttccttga agcgaaaatg cagggctaca accgtgcgcc cctttttgat cgtctcaacc
     3541 gttacagcca ggtcgctttt tttgttcagt tcgtcaacac agggttttat tagtatttta
     3601 tttaagtctc tgaattgttt gtatttattt tcaatcccta acattgaacg aaagtcatta
     3661 agagcaataa ttctctctcc cgtactgcgg aattgctgga ggaactcgta tattcttata
     3721 ctgtaactac ttgataaacc tgatatgttt tttataacaa cgcgcgtgaa ttgccccttc
     3781 agttgcgtca gataaggcat taccgcgtca gaaaaggtta ttcgagcctt ggcctcacct
     3841 ttagcgtact gtgcccgata ctggatccag cggaactcct cacgcttctc gtcattcttg
     3901 agaatgatcg acctgtccca taaacgatcg atcgcttcct gtaactgaac ctcggcattt
     3961 ttccgcccca tgtcagggaa tgaatcaaaa tattccgcag cggtaatggt cattgtcttt
     4021 tgcaattcag catcagcacc agatttcaat cgactgatac acagcaacag aa
//
