LOCUS       CP057710                6077 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB17-C03 plasmid pRHB17-C03_6, complete
            sequence.
ACCESSION   CP057710
VERSION     CP057710.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148143
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6077)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH03 T2-C03
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 76.2135419097413x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 11:06:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,795
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,437
            CDSs (with protein)               :: 4,437
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 124 of 239
            Pseudo Genes (incomplete)         :: 93 of 239
            Pseudo Genes (internal stop)      :: 41 of 239
            Pseudo Genes (multiple problems)  :: 17 of 239
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB17-C03"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB17-C03_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(6036..6077,1..492))
                     /locus_tag="HVX26_23925"
     CDS             complement(join(6036..6077,1..492))
                     /locus_tag="HVX26_23925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QML85498.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVTLED
                     KLAEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(495..1421)
                     /locus_tag="HVX26_23930"
     CDS             complement(495..1421)
                     /locus_tag="HVX26_23930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QML85492.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1597..1995
                     /locus_tag="HVX26_23935"
     CDS             1597..1995
                     /locus_tag="HVX26_23935"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QML85493.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(2189..3124)
                     /locus_tag="HVX26_23940"
     CDS             complement(2189..3124)
                     /locus_tag="HVX26_23940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QML85494.1"
                     /translation="MSAALQYFEENLPLRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDLNAPAPNITIKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAIERALCEKLGADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSTSA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTAETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDKRFMAL
                     CMLENGYSQKAIAAMLEVSTRTIRNWKSGK"
                     /name="replication initiation protein"
     gene            complement(3619..3762)
                     /locus_tag="HVX26_23945"
     CDS             complement(3619..3762)
                     /locus_tag="HVX26_23945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QML85495.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            complement(3824..4084)
                     /locus_tag="HVX26_23950"
     CDS             complement(3824..4084)
                     /locus_tag="HVX26_23950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="QML85496.1"
                     /translation="MELKHSISDYTEAEFLEFVKKICRAEGATEEDDNKLVREFERLTE
                     HPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKPGFKQG"
                     /name="bacteriocin immunity protein"
     gene            complement(4087..5814)
                     /locus_tag="HVX26_23955"
     CDS             complement(4087..5814)
                     /locus_tag="HVX26_23955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="QML85497.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENNP
                     WGGGSGSGVHWGGGSGHGNGGGNGNSGGGSNSSVAAVAFGFPALAAPGAGTLGIAVSGE
                     ALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPKMMSKIVTSLPAETVTNVQV
                     STLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASFPGVP
                     SLTVSTVKGLPVSTNLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKPIYVSV
                     TDVLTPAQVKQRQDEENRLQQEWNDTHPVEVAERNYEQARAELDQANKDVARNQERQAK
                     AVQVYNSRKSELDAANKTLADATAEIKQFERFAREPMAAGHRMWQMAGLKAQRAQTDVN
                     NKKAAFDAAAKDKSDADAALGAALERRKQKENKEKDSKDKLDKESKRNKPGKATGKGKP
                     VGDKWLDDAGKDSGVPIPDRIADKLRDKEFKSFDDFRKKFWEEVSKDPELSKQFKDSNK
                     TNIQKGKAPFARKKDQVGGRERFELHHDKPISQDGGVYDMDNIRVTTPKRHIDIHRGK"
                     /name="colicin"
     BGC             1..6077
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2190..3124)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.551"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(499..1421)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttatcttcc agtgtcacaa ctttctgata caccgccgct gtggcgttgt agttcctgtc
       61 gagcattgtt ttcattcccc atccaagcac acagaacata accagaaaag ccactgacac
      121 cccacccagc catttccagc tatacccttt aagggtgttg tattcctgca cgatattgct
      181 gtgaattgtc gcaatatccc tgttcagggc atccgcgttt gccttcatgt ttgcgactga
      241 ctcttccagc cgctgaaaat cctggctgat gcgctcacct atcagccctg caacatccag
      301 cttctgcacc tcgtttctga cttcatccct gacctgtttt gccacaccgt cagaaatatc
      361 ccgatatccg cgaagtgttt cccttgccac agccagagaa tcagcaacag ctttctgttg
      421 ctccattgcg gtatgttgca gatgctccac cgataacagg agcgtgagta atttttcttc
      481 tgtgctggcc atgttcagaa tcccatagaa aatccacgtg acggtgattt aacccgttca
      541 ggttcagttc ggttctgtct gctcactctg tctctcatgt caaagccatg ctcccgcgca
      601 acatcatgtt gcagccttct gaccagttca tgttgcttaa ccggagtgtc cagccattcc
      661 ctgatgtcat gcccgaccgt tcgcgcctgt tcaagatggc gggaatagtc cggttcagac
      721 cgtttttgca tgatgtcatg acgctgggca tcatcaagcc gtccggcagc ttcaaaaccc
      781 agatgcactt cgggacgtcg ttcaatgccc tgttctttca gcgtccggca gtcaatgaag
      841 cccctttcaa agccatgctt tctcatatgt tcgttgtgct tcacctccca gcgttttcgc
      901 aattcccgca actgctcttt ttgctgagtg agagtcaggc ttccggaatc ctttttcgca
      961 cctccccgct cgggatacct ggcgttatag cgccggaaat actgttccgg tgtacgttca
     1021 atgccatcat tcacacgctg gctcatcatg atatgcgcat gaggctgttc gccgccgtca
     1081 atgctcgctt tcggattgtg aatggcaaaa ctccaggcgt gccgttcgcc agcttcctga
     1141 cgtacaaaat cctgcaccag ttcaaggcgc tgttccgctg tcagttcacg gggtaatgca
     1201 atttcaagtt cccgataggt cgaaccgttc gcacgttcaa actggtcagc cgcctgccag
     1261 aaatgagagg gattatctcg cgcccattcc ggcatgtttc cgtgtgcgga atattccaaa
     1321 tcctgacggt cgcggtattt ctctttgcgc tcgatgtaat cggcgtggtt tgcagcctgc
     1381 cccttgccac caaatttaac gctgaggtga taggttgcca cggtttcagc cctccgcagg
     1441 agcgcactgt ttctcgacga aggagagaaa catataagtg cgcttccttc taattttacg
     1501 aattagcacg gaagataccc cctgcaccca ccgaccgcaa gcgatatact gcacgaccac
     1561 aacacacagc aaaaaacacc agggagcacc acgaacatgc gcatcagcaa catcgaatgg
     1621 ctgaagaaac ggatcggatt catcaggaag ctcggagaac agaccgcacg acagagacag
     1681 attattgacc tgctcgacaa cgaagcagga ctcacagaac aggaacgcaa actcctccac
     1741 gtactggcaa cagcggaaaa aaacgactta caggcacagg agagcgaacg aaaacaggcg
     1801 gtccaaaaac gaatcgaggg aaaaaaacaa cgacgagaac gcaatcaccg actctttctc
     1861 gcggctgggc tactgatcga agcaggactg gtagacacaa aaacaggcga gctatgctat
     1921 aaaaaagaca ggatattaca ggcacttaag gaaattaagt acgatctgga gacatcccca
     1981 aacccagacg cgtaagtgcc tccatccgtg ttccggttcc ctggtgcttc ctcgctcatt
     2041 cgctcgctac gctcggtcat gagactgcgg cgagcactac cagcacaatc atgcggaaaa
     2101 cagcaccatt gcagggctga acggggaaaa acggcaaaag acctactgct caaagaaacg
     2161 ccagaaaggc gctgttatct gatataggct attttccgct tttccagttt cgaatggttc
     2221 gagtagaaac ctccaacatc gccgcaatag ctttctgaga atatccattc tccagcatac
     2281 atagcgccat gaaacgcttg tcatcgtagg cttcgccttt agcgatgcca cctttcctgc
     2341 ctctcttggc ctgtatttct ggcgtgtgcg tatcagccac atactgtgcg aaagtttccg
     2401 ccgtgaagtt cctgtgcgtg tatttcgcaa tactcttgcc aatagcccga cattcaggag
     2461 gtgaaagcgg aacgggaagc gatgcgttgt acatttcgac acgctgaatc acggcatcaa
     2521 gccactgtga gaatgctggc cagccctgac gaatcgccct gtaagcccat ttgcgcgtca
     2581 tttcgaacag atggcagttt cgccccatcc cgtaatgttt atcgatgcta cggcgcgctg
     2641 aagtgctcaa atcgagataa tcagccagtt catcgagggt ataggcttcc tcacgccact
     2701 ccatcacctg ccattccaga tggaacggat ttttgcaaat cagtccgctg tagtttacat
     2761 ctgcgcccag tttttcacac aacgcacgtt caatcgcggc ggcgtatttc agcgccttaa
     2821 ccgaagcatc aggcgcggtt ctcacggcga tattgagtgc gtagagcagg tgggcgtgac
     2881 cgttcacggg attttttatg gtgatgttcg gtgcgggggc gttcaggtcg ctccagtcaa
     2941 tcgcagcccc ctcgcggtca acgtcaaaaa ccagccagaa ctgcgcatga ggttggttct
     3001 gctggatata ccgtgcaaga agcgcgcgcc ctttaccgga gatacgaagg ccgaaagcga
     3061 gatcatccgt gtgatagggg cggaggggta aattttcttc gaagtattga agcgcggcgc
     3121 tcatgtgtta cgattccttc taggttatcg accgacctcg cccctgtatt ctcgtgacaa
     3181 tcttggcgga gagacacgaa tttcaggggc gtttgttttt cagtcgctta cactacaccc
     3241 aacggcaaat cctgtcgatc cttcccgaaa acccgatcca tttaccataa ctcccgttgt
     3301 acgtatcctg taaaacgccc tcaatcgctt tctggcgcaa aaaacgcaaa aagacaagga
     3361 aacaccaggc taaaccagaa aaacgcgcca gaatcgtttt tagagggctg cataactatg
     3421 cacgaaagcg atgcgcgatc gatttcgact gttccgaagg gcgaacaggt caaaatgaca
     3481 agttttctcc ccctgggtgg aaagttctgc acggggattt tagctctcat ctcccgcatg
     3541 gaatttttgt gacatttcaa aaaaaagcgc atcagaatca gcctgacacg ctttgttttt
     3601 gtcaaagagg atttcgggtt actgcgttgc taatccggtc acttcagctg ttgatgacgg
     3661 ggatacagtc ccgccctgaa catcccggat atagtttgcc tgacatgcag ccagaataat
     3721 gactgcaaga agcaataaaa taatccctgt tatttttttc ataacgactc cttgttgtga
     3781 aagaatgcca gccattcatc cataaacaac cggcattcat attttagccc tgtttaaatc
     3841 ctggcttacc gttagcagct cgccattctt taatttcttt aacaatccct tcaggactat
     3901 cttccctgtc atcgcgagga taataaatca gatctgaacc atctgggtgc tcagttaatc
     3961 gctcaaactc tctcactaat ttattgtcat cctcttcagt agcaccttca gctctacata
     4021 ttttttttac aaactccaga aattcagcct cggtataatc actaatacta tgtttcagtt
     4081 ccatttttac ttacctctat gaatatcaat atgtcgctta ggtgtggtca ctctgatatt
     4141 atccatatca tagacaccac catcctgact gattggttta tcatgatgta attcaaagcg
     4201 ttccctacca cctacttggt ctttcttcct tgcaaaaggt gcttttccct tttgaatgtt
     4261 cgtcttatta ctgtctttaa attgcttact aagctcggga tcttttgaca cttcttccca
     4321 gaatttcttc cggaaatcgt caaaactttt aaattcttta tcacgcaact tatcagcaat
     4381 gcgatcagga attggcactc ctgaatcttt acccgcatca tcaagccatt tatcaccaac
     4441 tggttttcct ttacccgtcg ccttccctgg cttattccgt ttactctcct tatctaattt
     4501 atccttagag tccttttctt tattttcctt ctgcttacgg cgttccagcg cagcacctaa
     4561 cgcagcatca gcatcagact tatcttttgc agcagcatca aatgcagcct tcttattatt
     4621 tacatccgtc tgtgcccgct gggccttaag ccctgccatt tgccacattc ggtgaccagc
     4681 agccattggt tctcgggcaa atcgctcgaa ttgttttatt tcagccgttg catcagcaag
     4741 agttttattc gctgcatcaa gttcactttt acgagaatta taaacctgaa cagctttagc
     4801 ctgtcgttcc tggtttctgg caacatcttt attagcctga tccagttctg cgcgggcctg
     4861 ttcataattg cgttcagcca cttctacagg atgtgtgtca ttccattcct gctggaggcg
     4921 attttcctca tcctgacgtt gttttacctg tgcaggggta agaacatctg tcactgatac
     4981 ataaatcggc ttctgcccac tttctttcgg gaaacggatc accgcttcat gtgaaccacc
     5041 accaaaggta aatcctgcag gaacggcagt ccggccctta tcctccgtaa taccacgggg
     5101 aaggtttgtt gacacaggaa gacctttgac cgtgctgact gtcagagaag ggacaccagg
     5161 aaatgatgcg tgaaatacac ctggggtacg tgttggttta gcattcacaa caggcacact
     5221 cataggtaca cctgcaacta cggcaatatg ctgtcgtgtg tccttcacaa catcggtcac
     5281 acgttttgtc acgctaacag ttgcctggtc cagcggaagt gtactgacct gaacatttgt
     5341 tactgtttct gccggtaatg acgtcacaat ctttgacatc attttcgggt catcttttgc
     5401 tatttcagat ggcagaatgc cgtaaagcgc aataccccat gcactgaatt taaacggacc
     5461 tttcagggct gcaaagatat ccgctattgc cgcagataaa gcctcaccag agacggcgat
     5521 acccagtgtt ccggcaccag gagctgccaa agcaggaaaa ccaaatgcca ctgctgctac
     5581 ggatgaattg ctgccaccac cggaattacc attccccccg ccattgccat ggccggagcc
     5641 acctccccag tgaacaccac taccagaacc gccaccccat gggttatttt ccgaactcca
     5701 tccggagccg tcagaagccc caccatttcc accaagcccc gtagggccgc cattaatgtt
     5761 accacctgtg ttatgtgcgc cactgttgtg gtcgcgtcca tctccaccgc tcataaaaat
     5821 tccctctttt aagcgttaaa acaattgatt acatagttaa tactgtacat aaaaccactg
     5881 gttttatgta cagatcaaaa agtactcgcc attatttttt ctgtcaagtc agggggagga
     5941 tattttttgg cagttcggag gaatcactca tgaaaacggt gaacggaacg cgaaatcctt
     6001 tcgcgggtcg tgaggtgttg gacaagcgaa gcgcgtcagt ttttccggct cttgcccgtg
     6061 acaggtttct ccgccag
//
