LOCUS       CP057709                6988 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB17-C03 plasmid pRHB17-C03_5, complete
            sequence.
ACCESSION   CP057709
VERSION     CP057709.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148143
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6988)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH03 T2-C03
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 76.2135419097413x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 11:06:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,795
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,437
            CDSs (with protein)               :: 4,437
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 124 of 239
            Pseudo Genes (incomplete)         :: 93 of 239
            Pseudo Genes (internal stop)      :: 41 of 239
            Pseudo Genes (multiple problems)  :: 17 of 239
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB17-C03"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB17-C03_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(6765..6988,1..199)
                     /gene="exc2"
                     /locus_tag="HVX26_23870"
     CDS             join(6765..6988,1..199)
                     /gene="exc2"
                     /locus_tag="HVX26_23870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QML85482.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTA
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(683..916)
                     /gene="mbeD"
                     /locus_tag="HVX26_23875"
     CDS             complement(683..916)
                     /gene="mbeD"
                     /locus_tag="HVX26_23875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QML85483.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="mbeD"
     gene            complement(923..1483)
                     /locus_tag="HVX26_23880"
     CDS             complement(923..1483)
                     /locus_tag="HVX26_23880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QML85484.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(1455..2198)
                     /locus_tag="HVX26_23885"
                     /pseudo=""
     CDS             complement(1455..2198)
                     /locus_tag="HVX26_23885"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2161..2484)
                     /locus_tag="HVX26_23890"
     CDS             complement(2161..2484)
                     /locus_tag="HVX26_23890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QML85485.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2552..2743
                     /locus_tag="HVX26_23895"
     CDS             2552..2743
                     /locus_tag="HVX26_23895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QML85486.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3749..3880)
                     /locus_tag="HVX26_23900"
     CDS             complement(3749..3880)
                     /locus_tag="HVX26_23900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QML85487.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            3910..4200
                     /gene="cki"
                     /locus_tag="HVX26_23905"
     CDS             3910..4200
                     /gene="cki"
                     /locus_tag="HVX26_23905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="QML85488.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            complement(4197..5843)
                     /gene="cka"
                     /locus_tag="HVX26_23910"
     CDS             complement(4197..5843)
                     /gene="cka"
                     /locus_tag="HVX26_23910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="QML85489.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     gene            6083..6373
                     /locus_tag="HVX26_23915"
     CDS             6083..6373
                     /locus_tag="HVX26_23915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QML85490.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            complement(6365..6643)
                     /locus_tag="HVX26_23920"
     CDS             complement(6365..6643)
                     /locus_tag="HVX26_23920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QML85491.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     Typing          complement(2829..2916)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          2927..3483
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.633"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(577..2171)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.624"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2544..2746)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.706"
                     /coverage="100.0"
                     /evalue="3.86e-12"
ORIGIN
        1 agccggagta tcagcagaaa cggcacagca acgtgtaaaa gaattccact cagaaaaatt
       61 ttttcactca ctccggagca caacagcctt tgctggcaga aaatacacaa acagcgatat
      121 gccttcgccg aaaaaaatga aactaatggc agacaccatt tctgcggttt atctcgatgg
      181 atacgagggc agacagtaag ggatttacca taatccctta attgtacgca ccgctgaaat
      241 gcgttcagcg cgatcacggc tgctgacagg taaaaatggc aacaaaccac ccgaaaagct
      301 gccgcgatcg cacctgataa attttaaccg tatgcatagc tattcagcca tgtgaataac
      361 gctagttttg cctgcgtaaa cctcatgaca ctgttttttt tccatctttt cagttgatga
      421 catacgcaga catcgcggga tgaggctgag gaatgagcgc gatctggcaa agaggcaaaa
      481 cacagcaaca aaaacgacac gccagaatcg cgcccggatg cgtttttaac gcgttccggt
      541 accatctggc aacctcccgg aacaactcac cgtcacatcc ctattgacgg gccacgccat
      601 acccgtgctt cccgttcctg ctcttcatgc caggaccgcg cacgctcccg ttccaggcgt
      661 gcctgccttt cctgttcatc ccttatctgc tgttcgtgat aaataaccga ctcaagtggt
      721 ccacctgccc ggctaatctc tgcacctgct gactcaagtc gtcgcactgt tccctcagtt
      781 gcccgttctc ctgtcgtgtc agctcgaaca tatgctgcaa atccgtgaag gcgctctccc
      841 agtctttcag ccgctgcgta tagtcctgct gcaattgctc taaggcgttc agtaagtgca
      901 tttccagctc tgtcatactc acttactccc tgaccagtct tactgcgttc ttcttctcca
      961 ccgtccagtt gttttccccc ttcaccccgg acggcaacac cagaaatttc ccgttcctgc
     1021 cctcgtgata cgtcacaccc catgtttttt cccggagttt cgccagcgtc tcttcctggt
     1081 ccctgatagc caggatgttc gccgcaatcc ggctttcctg ccactgaatc agcccccatg
     1141 acgccagaaa aatcccgccc gtgacgcaaa gcgccgtcag cgacgggtac agtatccgcc
     1201 ctttgaccag cttccagagc agctcttcct gccgccgggc cagttcgttc tctgtggcgc
     1261 tgaactgcgc gttcacggca ctgttcagcg tctccagttg ttctttcacc gctgctgtgt
     1321 gtgcgctgat agcgtctctg attttctgcc cgtttaagtt cagttccctg tctacagacg
     1381 cttcgagctt cctgaactcg ctgttcagca tgttctctgt agagacggca cgctctttca
     1441 gtttcttctc gaagtctgtc cccatttgta aaagattgct catacagcgc ccctttcagc
     1501 ctgagattac gcccaccctc cgggtcggcg atactgatac tgctcctggt tgtcctcaca
     1561 acctcaaaac ctgccgctgt aagcgcctca gtgacatcct gacgcgtttt tagcgctccg
     1621 gcatggtaaa gagcctccag tcccctcgta atcgcttctg cggcctcctg tttcgctttc
     1681 ggcagattat tcggggtgac aagtgtccgc ctgttctccg gtgcgttcgg gtcgtgcagc
     1741 ccgtaatggt gattcaccag tgtctgccag gcattgattc gcggacggtc tgctcggtcg
     1801 taatagggct ggagccgttt tccgctcgcc agctccatat tcgggatgac aaaattcagc
     1861 tcaagacgcc ccttgtcctg gtgctccacc cacaggatgc tgtactgatt tttttcaaga
     1921 ccgggcatca gtacccgctc aaagctctcc atcacccttt cacgctctcc cggtggcagg
     1981 gtctgctctg caaaagacag aacccccgag gtgtattttt tcgcaaacgg cgtggcatcg
     2041 atgagttccc gcacctcttc gggagcaccc cgcagaactc tcgccccttc ccggttacgc
     2101 tcccggccca gcaggtaatc aaccggacca ctgccaccgc cttttcccct ggcatgaaac
     2161 ttaactatca tcccgttctc cctgtttacg gacctcatcc ctcagctcac tcagttcacg
     2221 tccgatggcc atcagtgcag ccaccacatg aacccggtca tgccccgacc actgtccgct
     2281 gtttatcttc cgggctatct gattcaggtt attgccgacc gaagcgaact ggcgcaacag
     2341 cggcggtgcc agtgtcggaa gacctgacgt tttcgatggc ggtgccccca ggcagacctt
     2401 acgcatccat gacgcaagtt gttttccctc acaacgtgcc agcagccgcg catgttcctc
     2461 atccgtgacc cgtatcgtga gcatcctttc gcgtttcacc ggtatcatta aaaacctccg
     2521 acagactccc cacacatgga gaaacagaac tgtgactaaa caggaaaaaa ccgcccttaa
     2581 catggcccgc ttcatcagaa gccagacgct gaccctgctg gaaaaactga atgaactgga
     2641 cgccgacgac caggctgaca tctgcgaagc gcttcacgac cacgctgacg agctttaccg
     2701 cagctgcctc gcacgcttcg gggatgacgg tgaaaacctc tgacacatgc agctcccgga
     2761 gacggtcaca gctgacctgt gagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     2821 agcgggtttt agcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
     2881 gtatactggc ttaatcattt ggcatcaatg aggattgtat gaaaagtgca ccatgccggg
     2941 tgtgaaatgc cgcacagatg cgtaaggaga aaatgctcgt ccaggcgctt ttccgcttcc
     3001 tcgctcactg actcgctccg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     3061 aagacggtaa tgcggttatc cacagaatca ggggataaca ccaaaagaaa catgtgagca
     3121 aaaaacaaga acccggaaaa ggccacgcag ctggcgtttt tccataggct ccgcccccct
     3181 gacgagcatc acaaaaaccg acgctcaagt cagaggtggc gaaacccgac aggacttaaa
     3241 gataccaggc gtttccccct ggtggctccc tcgtgcgctc tcctgttccg accctgccgc
     3301 ttaccggata cctgtccgcc tttctccctt tgggaagcgt ggcgctttct catagctcac
     3361 gctgttggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3421 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     3481 taagacacga cttaacgcca ctggcagcag ccattggtaa ctggatagtg gatttagata
     3541 cgcagaactc ttgaagttga agccttatag cggctacact ggaaggacag catttggtat
     3601 ctgtgctcca ctaaagccag ttacccggtt aagcagtccc caactgactt aaccttcgac
     3661 taaaccgcct ccccaggcgg ttttttcgtt tacaggcagc agattacgcg cagaaaaaaa
     3721 ggatctcaag aagatccttt gatcttttct actgaaccgc gatccccgtc agttcagaag
     3781 acgaagatgg tgcaacggtt cctccttgta catcacgaat atagtttgct tgacatcctg
     3841 acaagaataa tattgtaatt agaaaaatta ttattttcat atttctttcc aacaaaagta
     3901 aaaatactta tgcatttaaa atactaccta cataatttac ctgaatcact tataccatgg
     3961 attcttattt taatatttaa cgacaatgat aacactcctt tgttatttat atttatatca
     4021 tcaatacatg tattgctata tccatactct aaattaacca tatctagata tatcaaagaa
     4081 aatacaaagt taaaaaaaga accctggtac ttatgcaagt tatctgcatt gttttattta
     4141 ttaatggcaa tcccagtagg attgccaagt ttcatatatt acactctaaa gagaaattaa
     4201 atccctaaca actcattaag tttgctcaac tcatcatccc ctatgtaaga agaaacaata
     4261 cctgtgacaa ttgcaatccc ccagaaacca agaggcacac caacaataaa actaaacatt
     4321 aatgccacaa tttttgccac acctacatcg accgcacttt tctccagcgt gacaaacaaa
     4381 ggtcgccagt taccagtttc tattgcattt ttaaaatcag aaccaacatc atatagaaaa
     4441 gatactcgac tggtgatttt aagcgacttt gatatcttcg ttaaattctt agataactca
     4501 tcataattaa cagactctaa cgcattaaaa atagcatccc gatcaacctt actgaatttc
     4561 ttatccagta cattcttata cttttcaaat gctgccagag cttcatcaac accttgaatt
     4621 ttctttcctt tagacttttc cgctaaatcc tgagcaattt ttgcgtattt ttctccatat
     4681 tgttcggtta tatattgata aaatccaacc atagtatcaa cagcatcttt tatctggctc
     4741 ttttcaagag catcctgagc ttcttttaat ttctgctctg cctctttaac ctcagcggcc
     4801 ttaccatccc tgaaactaac agcattctta agttcatttt caactgcgga gtactcagcc
     4861 tgcttagctt caagctgacg ctgtagtttt ttctgaacgt cacgccatcc cggaaatccg
     4921 gtgaccttca cgtcaacact cttctgagcg ttttcaagct ctcctgcaac tctggaaaca
     4981 gtagcttgct tactctgtac atcactttca gccttcgcca gttcagcttt cgcctcagct
     5041 aaacgcttct ctgcttccgc caccgcctgt ttctcttcat taagagtttc atgttcagcg
     5101 acctctgcat tttcacgagc cttttcttca gcttccttct gtttattcac atcaccaatt
     5161 ttactttgca atgtattttt ataagaatta agtttgctaa tatctgcatc aagctgattt
     5221 gatttttttt gcaactcatc aacatccctc tcaagatcag tgataccatg ataagaatga
     5281 tgcttgaata cttttttcat ctcctcgatt ttcttctgtt tttcactaat ctgtgtagca
     5341 attttattct tctgcttttg tttttcatta attacattac ccaccacctt cgaactctta
     5401 tccatatcac tgacctgagc attcgttggt gcagtattaa ctgaagcagt gttattgttg
     5461 ttatttcctg agctttctgc aaaaagtgat ggcatatcac taattaaaga attaagaact
     5521 ctggagaccc ctccaaatgg attatcaacc agagttgaat tctcttctgt cataacaata
     5581 ccattcatca ccggaaggcc atcattatta atgacaacat caccccacgg agtcagatat
     5641 gactcaccgg ttttcatcac agttgatgta gaaccagatg aatttgaatt tccctgacca
     5701 ccattattac catgcccaga gccaccaccc cagtgaaccc cactattact attattattt
     5761 ccaccttgct gattcagatt cgcccctgtt ccccccatag actcaccagc agttggtcca
     5821 tatccactta gttctttagc cataaattcc tctttgataa ttaaaacaat aaattaaaaa
     5881 caatatactg tatatataac cactggtttt atatacagta aaacctacta ctcagcattg
     5941 tccatgtcaa gagcatggat tttcattttt gcaataagga tcacactatg gggaggcagg
     6001 cattgagaac gtcgaaacag aacaccggag caaatcagga tgagatataa aactgttgga
     6061 tcatgaaaaa acggagaacg atgtgagcaa atcaccccgc cataaactga acaaaacaga
     6121 caaacgactt ctcgacaccc ttgttgctgc cggatatgag catgacaaag cccgtgacct
     6181 catccagaaa caggtttaca cgctgacact ggctgatcag cgtcatgtgg tcagtgaaat
     6241 cagtaatggt gtgaatccca cccaggctta ctcggcggta taccaggcaa gacgcattcg
     6301 cctcgcccgt aaatatctga acggtaaaaa ggttatggaa gaaaccgggg aaaatacgcc
     6361 cccatcagcg taaggatttc ttttgccgct ccagagactc cagtttttta cgcaaatcct
     6421 ctcttttttg ggcatctctg gtgccagcca gctctgctct caactcatcg atctgaagtt
     6481 gtatcttcag tctgttactg aacattttct gtctggcatt aacatccgca acaatgccgt
     6541 tttttgtctt ctcggccttt tgttgaaaaa cattgctgtc cgcatgactg gcaaccgaag
     6601 cagaaagaac actaaaaagc aggactggca cacatttttt cacgggatta ttcctgactc
     6661 attgaccatc aaatcacatt gggagtaaac cgacgtatga taagagatac tcttcggaga
     6721 tataactccc tgagtatcaa gattaaaaac gcaaggagat gtttatgaga tctgccgctg
     6781 ccaggctgct tctgatacct ctgataacag caacaatagc tcttacagga tgcacaccaa
     6841 agaccagcct ggaacgacat acccggcatt atgtttatgc ttcagatgat ggatttgatc
     6901 ctaacttcta cacccagaaa gcagacacca tacgtatgat gctcccgttc tttcagcagt
     6961 tccgggatat ggggatgaaa gacaaagc
//
