LOCUS       CP057703                2442 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB17-C09 plasmid pRHB17-C09_8, complete
            sequence.
ACCESSION   CP057703
VERSION     CP057703.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148144
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH03 T2-C09
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 84.5809268322776x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 11:12:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,029
            CDSs (total)                      :: 4,911
            Genes (coding)                    :: 4,700
            CDSs (with protein)               :: 4,700
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 211
            CDSs (without protein)            :: 211
            Pseudo Genes (ambiguous residues) :: 0 of 211
            Pseudo Genes (frameshifted)       :: 104 of 211
            Pseudo Genes (incomplete)         :: 105 of 211
            Pseudo Genes (internal stop)      :: 43 of 211
            Pseudo Genes (multiple problems)  :: 38 of 211
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB17-C09"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB17-C09_8"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(2434..2442,1..960)
                     /locus_tag="HVX27_25130"
     CDS             join(2434..2442,1..960)
                     /locus_tag="HVX27_25130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="QML81005.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="plasmid recombination protein"
     gene            complement(1030..1260)
                     /locus_tag="HVX27_25135"
     CDS             complement(1030..1260)
                     /locus_tag="HVX27_25135"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QML81006.1"
                     /translation="MMNAEDKLFKNVDRLMLHDSLKKNEVLTVWERTFLSSVYSIYRRT
                     SGNKYSTKGLSPKQKNAAFSILKKHGYHLND"
                     /name="hypothetical protein"
     gene            complement(1247..1750)
                     /locus_tag="HVX27_25140"
     CDS             complement(1247..1750)
                     /locus_tag="HVX27_25140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QML81007.1"
                     /translation="MAKRGAGVNTKKISRLERHTYMDENGEVKQDSKIETFSIPAEPPY
                     VKLYIADIAMIHGLSKSQNDVLLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKN
                     IIAALVDKQIFFRAGRGELEANPFLFARGDWPSIYERRKKIELKISYDTNGRTVQTKVM
                     DDEC"
                     /name="hypothetical protein"
     Typing          2..957
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.611"
                     /coverage="94.0"
                     /evalue="0.0"
ORIGIN
        1 tacgcgataa tgcgctgcaa aaagctgacg ggaatgggaa gcgtggccag cgcgttgcag
       61 cactgctacc gagagcgtga gacgccgaac gctaacgctg aacggacacc agaaaactac
      121 tgctctgtgt ccaaatcaac ggatcaggca atggggcgtg tgcgggagct gctgccggaa
      181 aaacgccgta aagatgcggt actggccgtt gaatacgtta tgacagcaag ccctgagtgg
      241 tggaaagagg ccacaccacg gcagcaggcg gagtttttcg cccgctctga gcaatggctg
      301 gagaagaaat acgggaagga tcgtgttgtg gcggcggtgg ttcaccggga cgaggccacc
      361 ccgcacctgt ctgcgttcgt ggtgccattg acgcaggatg gacggctgtc cgcgaaagag
      421 tttattggcg ggcggtcaaa gatgcgtgaa gatcagagca cctacgctga atcagtaaaa
      481 aaactgggtc ttgagcgcgg catcgaggga agccgcgcca cgcatcagac agttcagcac
      541 tactacgaat cgataaaccg tggcacccgt agccaggtat cgatctcgcc ggaggcgctg
      601 gagccgcgtg tattgcggaa gggcattttc accaaagacg tggaagatca ggcagccata
      661 gccaaacgtc taagccacgc agtgaacgat ggctttgcag gaaccatcgc tatggcctct
      721 cagagcgccc agaacgcaaa aagagcgcgt gagctacaga agactatgga tgcgcagcag
      781 aaacgcctac agagcgtcac agagcctttt aaggggcttt ccagagagca gatgaccgaa
      841 atcctgatga tggctcagcg gtttaagcag caaaaccagg aaaaggaaaa acagcagcgt
      901 attgaacgtg aaaaacagcg acagacgcga tcgcgtggca tgggtggtat ggagcgttag
      961 ttttgatgcg atgcaggata gggctaacgc cgcaaagtga cgtcatttgc ggcattcacc
     1021 acgaaaaaat taatcattca gatggtaacc atgttttttt aaaatagaaa aagctgcgtt
     1081 tttctgcttt ggtgacaaac ccttagttga gtatttatta ccgctagttc tacgatatat
     1141 gctgtaaaca ctgcttaaaa aagtacgctc ccaaacagtc agaacctcat tcttttttag
     1201 tgaatcatgc agcattagcc tgtctacatt tttaaaaagt ttatcttcag cattcatcat
     1261 ccattacctt cgtctgcacc gttcttccat tggtgtcata actgatcttt aactcaattt
     1321 tctttcttct ttcgtaaatt gatggccagt ctccccgtgc aaaaagaaag gggttagctt
     1381 caagttcccc acgccctgcc cggaagaata tttgtttatc tacaagagcc gcaattatgt
     1441 ttttaaaggt ctgatattca atatgaaccc tgggctgtat ggttttttca aatctagttt
     1501 ttgatacaga cacaaaccca tcccatccaa tcagcattgc caattgtaaa agcacatcat
     1561 tttgagattt tgataaacca tgaatcatcg caatgtctgc aatgtatagc ttgacgtagg
     1621 gaggttcggc tgggatggaa aaggtctcga tcttagagtc ttgttttacc tcaccgtttt
     1681 catccatgta tgtatgccgt tcaagcctag aaatcttttt cgtattcact ccagccccac
     1741 gtttcgccat gatttagtac tcgcatttaa gtactaaaga gacatacagt caagtccttt
     1801 gtatcaagta cgattagtac ttgaggtgag tacgattagt ccttgaggtg agtacgatta
     1861 gtacttgagg tgagtacgat tggtacttga ggtgagtacg attcttcgct ctagcccttg
     1921 gtacaagcgg gttcccatat ctatatctat atattaaggg ggaaaataag ggctgaaaaa
     1981 cggcattttt agacactgaa aaactgcctt tttttagcaa ggagtgtacg catttctatg
     2041 tacggggagt ggaaattccg cttcctcgct ttggctgttc gttcctcgtc gcgttgctcc
     2101 tgcggtacgc ctcagccaaa gctgcggcga gcggttcctg ctctttcaaa gacgatttca
     2161 ttgcctgatt tcgtcaaggg cgagcgtagc gagtgccatt tacccttgac gacactccgg
     2221 ataaaacagg cttggcagtg gtggggggtg gcgcgaagcg acgccttcca cctgccagac
     2281 cgccgttgat tttgaccttc tggcagcttg ctgcccgaac gattttgcgt agcaaagtct
     2341 agtgagtaca ctatcatctt gatagtggcc cgccggaggt atcgcaatgc gctcccccgc
     2401 ctggctgaat gaatgccgga atcagggggg aacatggcga ac
//
