LOCUS       CP057672                6229 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB18-C18 plasmid pRHB18-C18_4, complete
            sequence.
ACCESSION   CP057672
VERSION     CP057672.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148159
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6229)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH13 T2-C18
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 82.3137058064701x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 10:54:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,918
            CDSs (total)                      :: 4,796
            Genes (coding)                    :: 4,707
            CDSs (with protein)               :: 4,707
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 35 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 10 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB18-C18"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB18-C18_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(6181..6229,1..971)
                     /locus_tag="HVX42_24560"
     CDS             join(6181..6229,1..971)
                     /locus_tag="HVX42_24560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QML36292.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKC"
                     /name="replication initiation protein"
     gene            complement(1232..1630)
                     /locus_tag="HVX42_24565"
     CDS             complement(1232..1630)
                     /locus_tag="HVX42_24565"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QML36293.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQENERKQAVQNRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            1807..2733
                     /locus_tag="HVX42_24570"
     CDS             1807..2733
                     /locus_tag="HVX42_24570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QML36294.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIEGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFETAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREYGFD
                     MRDGVSRQERTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            2736..3269
                     /locus_tag="HVX42_24575"
     CDS             2736..3269
                     /locus_tag="HVX42_24575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QML36295.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            3490..5217
                     /locus_tag="HVX42_24580"
     CDS             3490..5217
                     /locus_tag="HVX42_24580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="QML36296.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENNP
                     WGGGSGSGVHWGGGSGNGNGGGNGNSGGGSNSSVAAVAFGFPALATPGAGTLGIAVSGE
                     ALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVTNVQV
                     STLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASFPGVP
                     SLTVSTVKGLPVSTTLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKPIYVSV
                     TDVLTPAQVKQRQDEENRLQQEWNDTHPVEVAERNYEQARAELDQANKDVARNQERQAK
                     AVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQRAQTDVN
                     NKKAAFDAAAKDKSDADAALGAALERRKQKENKEKDSKDKLDKESKRNKPGKATGKGKP
                     VGDKWLDDAGKDSGAPIPDRIADKLRDKEFKSFDDFRKKFWEEVSKDPELSKQFKDSNK
                     TNIQKGKAPFARKKDQVGGRERFELHHDKPISQDGGVYDMDNIRVTTPKRHIDIHRGK"
                     /name="colicin"
     gene            5220..5480
                     /locus_tag="HVX42_24585"
     CDS             5220..5480
                     /locus_tag="HVX42_24585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="QML36297.1"
                     /translation="MELKHSISDYTEAEFLEFVKKICRAEGATEEDDNKLVREFERLTE
                     HPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKPGFKQG"
                     /name="bacteriocin immunity protein"
     gene            5542..5685
                     /locus_tag="HVX42_24590"
     CDS             5542..5685
                     /locus_tag="HVX42_24590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QML36298.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     BGC             1..6229
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2..727
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.149"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          1808..2730
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.403"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atcacacgga tgatctcgct ttcggccttc gtatctccgg caaagggcgc gcgcttcttg
       61 cacggtatat ccagcagaac cagcctcatg cacagttctg gctggttttt gacgttgacc
      121 gcgagggggc tgcgattgac tggagcgacc ggaacgctcc cgcgccgaac atcaccgtta
      181 aaaatcctgt gaacggacac gctcacctgc tctacgcact caacatcgcc gtgagaaccg
      241 cgcctgatgc ctcggttaag gcgctgaaat atgccgcagc ggttgaacgt tcgctgtgtg
      301 agaaactttg tgcggatgtg aactacagcg gcctgatttg caaaaatccg ttccacctgg
      361 aatggctggt gatggagtgg cgcgaggaag cctataccct cgatgaactg gctgattatc
      421 tcgatttgag cgcctcagag cgtcgtagca tcgataaaca ttacgggatg gggcgaaact
      481 gccacctgtt cgaaatgacg cgcaaatggg cttacagggc gattcgtcag ggctggccag
      541 cgttctcaca gtggcttgaa gcggtgatcc agcgtgtcga aatgtacaat gcatcgcttc
      601 ccgttccgct ttcactggct gaatgtcggg ctattggcaa gagcattgcg aaatacacgc
      661 acaggaactt cacgccggaa actttcgcac agtatgtggc tgatacgcac acgccagaaa
      721 ttcaggcagc acgcggtcgc aagggcggga aggccaattc gtcagaaaat cagtcggaca
      781 aagggaaaaa aagcgccgct gttcggtgga ctgctaacga cgacaaacgt cgtagggcgc
      841 ttgatatgta tatcttggga gcgtcaacgg aagatattgc agtggcggtt ggtgttagca
      901 gccggactat tcgacgctgg atggataatt ctggagaatg gctaaccaag aaacaaataa
      961 ttaaatgtta gttaacaaag aagcaacact accccccgtt ttttgttaat gggatgttaa
     1021 attcgcggac atgaacaagc ctatatcaga taacagcgcc tttcggcgtc tttttgagca
     1081 gcgggctttc cggcggtttt ctcctctcag cccagcaatg gtgcggctta ccgcatgatt
     1141 gaggtggtcg tgctcgccgc agtctcatga ccgagcgtag cgagcgaatg agcgaggaag
     1201 caccagggaa ccggaacacg gatggaggca cttacgcgtc tgggtttggg gatgtctcca
     1261 gatcgtactt aatttcctta agtgcctgta atatcctgtc ttttttatag catagctcgc
     1321 ctgtttttgt gtctaccagt cctgcttcga tcagtagccc agccgcgaga aagagtcggt
     1381 gattgcgttc tcgtcgttgt ttttttccct cgattcgatt ttggaccgcc tgttttcgtt
     1441 cgttctcctg tgcctgtaag tcgttttttt ccgctgttgc cagtacgtgg aggagtttgc
     1501 gttcctgttc tgtgagtcct gcttcgttgt cgagcaggtc aataatctgt ctctgtcgtg
     1561 cggtctgttc tccgagcttc ctgatgaatc cgatccgttt cttcagccat tcgatgttgc
     1621 tgatgcgcat gttcgtggtg ctccctggtg tttttttgcg gtgtgttgtg gtcgtgcagt
     1681 atatcgcttg cggtcggcgg gtgcaggggg tatcttccgt gctaattcgt aaaattagaa
     1741 ggaagcgcac ttatatgttt ctctccttcg tcgagaaaca gtgcgctcct gcggagggct
     1801 gaaaccgtgg caacctatca cctcagcgtt aaatttggtg gcaaggggca ggctgcaaac
     1861 cacgccgatt acatcgagcg caaagagaaa taccgcgacc gtcaggattt ggaatattcc
     1921 gcacacggaa acatgccgga atgggcgcga gataatccct cacatttctg gcaggcggct
     1981 gaccagtttg aacgtgcaaa cggttcgact tatcgggaac tggagatagc gctgccccgt
     2041 gaactgacac cggaacagcg ccttgaactg gtgcaggatt ttgtacgtca ggaagctggc
     2101 gaacggcatg catggagttt tgccattcac aatccgaaag cgagcattga gggaggcgaa
     2161 cagcctcatg cgcatatcat gatgagtcag cgtgtgaatg atggcattga acgtacaccg
     2221 gaacagtatt tccggcgcta taacgccagg tatcccgagc ggggaggtgc gaaaaaggat
     2281 tcaggaagcc tgactcccac tcagcaaaaa gagcagttgc gggaattgcg aaaacgctgg
     2341 gaggtgaagc acaacgaaca tatgagaaag catggctttg aaaggggctt cattgactgc
     2401 cggacgctga aagaacaggg cattgaacga cgtcctgaag tgcatctggg ttttgaaact
     2461 gccggacggc ttgatgatgc ccagcgtcat gacatcatgc aaaaacggtc tgaaccggac
     2521 tattcccgcc atcttgaaca ggcgcgaacg gtcgggcatg acatcaggga atggctggac
     2581 actccggtta agcaacatga actggtcaga aggctgcaac atgatgttgc gcgggagtat
     2641 ggctttgaca tgagagacgg agtgagcaga caggagcgaa ctgaacctga acgggttaaa
     2701 tcaccgtcac gtggattttc tatgggattc tgaacatggc cagcacagaa gaaaaattac
     2761 tcacgctcct gttatcggtg gagcatctgc aacataccgc aatggagcaa cagaaagctg
     2821 ttgctgattc tctggctgtg gcaagggaaa cacttcgcgg atatcgggat atttctgacg
     2881 gtgtggcaaa acaggtcagg gatgaagtca gaaatgaggt gcagaagctg gacgtggcag
     2941 gactgatagg tgagcgcatc agccaggatt ttcagcggct ggaagagtca gtcgcaaaca
     3001 tgaaggcaaa cgcggatgcc ctgaacaggg atattgcgac aattcacagc aatatcgtgc
     3061 aggaatacaa cacccttaaa gggtatagct ggaaatggct gggtggggtg tcagtggctt
     3121 ttctggttat gttctgtgtg ctcggatggg gaatgaaaac aatactcgac aggaactaca
     3181 acgccacagc ggcggtgtat cagaaagttg tgacactgga agataaactg gtggagaaac
     3241 ctgtcacggg taagagccgg aaaaactgac gcgcttcgct tgtccaacgc ctcacgaccc
     3301 gcgaaaggat ttcgcgttcc gttcaccgtt ttcatgagtg attcctccga actgccaaaa
     3361 aatatccccc cctgacttga cagaaaaaat aatggcgagt actttttgat ctgtacataa
     3421 aaccagtggt tttatgtaca gtattaacta tgtaatcaat tgttttaacg cttaaaagag
     3481 ggaattttta tgagcggtgg agatggacgc gaccacaaca gtggcgcaca taacacaggt
     3541 ggtaacatta atggcggccc tacggggctt ggtggaaatg gtggggcttc tgacggttca
     3601 ggatggagtt cggaaaataa tccatggggt ggcggttccg gtagtggtgt tcactgggga
     3661 ggtggctccg gcaatggtaa tggcgggggg aatggtaatt ccggtggtgg cagcaattca
     3721 tccgtagcag cagtggcatt tggttttcct gctttggcaa ctcctggtgc cggaacgctg
     3781 ggtatcgccg tctctggtga ggctttatct gcggcaatag ctgatatctt tgcagccctg
     3841 aaaggtccgt ttaaattcag cgcatggggt attgcgcttt acggcattct gccatctgaa
     3901 atagcaaaag atgacccgaa tatgatgtca aagattgtga cgtcattacc ggcagaaaca
     3961 gtgacgaatg ttcaggtcag tacacttccg ctggaccagg caactgtcag cgtgacgaaa
     4021 cgtgtgacag atgttgtgaa ggacacacga cagcatattg ccgtagttgc aggtgtgcct
     4081 atgagtgtgc ctgttgtgaa tgccaaacca acacgtactc caggtgtatt tcacgcatca
     4141 tttcctggtg tcccttctct gacagtcagc actgtcaaag gccttccggt gtcaacaacc
     4201 cttccccgtg gtattacgga ggataaaggc cggactgccg ttcctgcagg atttaccttt
     4261 ggtggtggtt cacatgaagc ggtgatccgt tttccgaaag aaagtgggca gaagccgatt
     4321 tatgtatcag tgacagatgt tcttacccct gcacaggtaa aacaacgtca ggatgaggaa
     4381 aatcgcctcc agcaggaatg gaatgacaca catcctgtag aagtggctga acgcaattat
     4441 gaacaggccc gcgcagaact ggatcaggct aataaagatg ttgccagaaa ccaggaacga
     4501 caggctaaag ctgttcaggt ttataattct cgtaaaagtg aacttgatgc tgcgaataaa
     4561 actcttgctg atgcaaaggc tgaaataaaa caattcgagc gatttgcccg agaaccaatg
     4621 gctgctggtc accgaatgtg gcaaatggca gggcttaagg cccagcgggc acagacggat
     4681 gtaaataata agaaggctgc atttgatgct gctgcaaaag ataagtctga tgctgatgct
     4741 gcgttaggtg ctgcgctgga acgccgtaag cagaaggaaa ataaagaaaa ggactctaag
     4801 gataaattag ataaggagag taaacggaat aagccaggga aggcgacggg taaaggaaaa
     4861 ccagttggtg ataaatggct tgatgatgcg ggtaaagatt caggagcgcc aattcctgat
     4921 cgcattgctg ataagttgcg tgataaagaa tttaaaagtt ttgacgattt ccggaagaaa
     4981 ttctgggaag aagtgtcaaa agatcccgag cttagtaagc aatttaaaga cagtaataag
     5041 acgaacattc aaaagggaaa agcacctttt gcaaggaaga aagaccaagt aggtggtagg
     5101 gaacgctttg aattacatca tgataaacca atcagtcagg atggtggtgt ctatgatatg
     5161 gataatatca gagtgaccac acctaagcga catattgata ttcatagagg taagtaaaaa
     5221 tggaactgaa acatagtatt agtgattata ccgaggctga atttctggag tttgtaaaaa
     5281 aaatatgtag agctgaaggt gctactgaag aggatgacaa taaattagtg agagagtttg
     5341 agcgattaac tgagcaccca gatggttcag atctgattta ttatcctcgc gatgacaggg
     5401 aagatagtcc tgaagggatt gttaaagaaa ttaaagaatg gcgagctgct aacggtaagc
     5461 caggatttaa acagggctaa aatatgaatg ccggttgttt atggatgaat ggctggcatt
     5521 ctttcacaac aaggagtcgt tatgaaaaaa ataacaggga ttattttatt gcttcttgca
     5581 gtcattattc tggctgcatg tcaggcaaac tatatccggg atgttcaggg cgggactgta
     5641 tccccgtcat caacagctga agtgaccgga ttagcaacgc agtaacccga aatcctcttt
     5701 gacaaaaaca aagcgtgtca ggctgattct gatgcgcttt ttttttgaaa tgtcacaaaa
     5761 attccatgcg ggagatgaga gctaaaatcc ccgtgcagaa ctttccaccc agggcgagaa
     5821 aacttgtcat tttgacctgt tcgcccttcg gaacagtcga aatcgatcgc gcatcgcttt
     5881 cgtgcatagt tatgcagccc tctaaaaacg attctggcgc gtttttctgg tttggcctgg
     5941 tgttttcttg tctttttgcg ttttttgcgt cagaaagcga ctgagggcgt tttaaggggt
     6001 gcgtacaacg ggagttatgg taaatggatc ggatttgcgg gaaggctcga caggttttgt
     6061 cgttgggggt agtgtaagcg actgaaaaac aaacgcccct gaaattcgtg tctctccgcc
     6121 aagactttca cgaaaataca ggggcgatgt cggtcgataa cctggaagga atcgtaacac
     6181 atgagcgccg cgcttcaata cttcgaagaa aatttacccc accgtccct
//
