LOCUS       CP057671                6930 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB18-C18 plasmid pRHB18-C18_3, complete
            sequence.
ACCESSION   CP057671
VERSION     CP057671.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148159
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH13 T2-C18
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 82.3137058064701x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 10:54:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,918
            CDSs (total)                      :: 4,796
            Genes (coding)                    :: 4,707
            CDSs (with protein)               :: 4,707
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 35 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 10 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB18-C18"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB18-C18_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(391..546)
                     /gene="csl"
                     /locus_tag="HVX42_24505"
     CDS             complement(391..546)
                     /gene="csl"
                     /locus_tag="HVX42_24505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="QML36282.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GSSGRL"
                     /name="csl"
     gene            576..1115
                     /gene="csi"
                     /locus_tag="HVX42_24510"
     CDS             576..1115
                     /gene="csi"
                     /locus_tag="HVX42_24510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 immunity protein"
                     /protein_id="QML36283.1"
                     /translation="MMIDEHSIDIDNRKANNLLYLFMVIGVIPLLCILAVYYTNPDNLF
                     LHTIATSTENIPSITSAYNPLMTKVMDIYCKTAPSLALILFILTFKTRKLIKKTNRNAV
                     LRSCLLSPFVCAVFLYLLCFRNLELTTAGRPARLMANNDATLLLFYIGLYLIIFFISYF
                     TLFTPVTTFKLLKERQ"
                     /name="csi"
     gene            complement(1143..2642)
                     /gene="csa"
                     /locus_tag="HVX42_24515"
     CDS             complement(1143..2642)
                     /gene="csa"
                     /locus_tag="HVX42_24515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /protein_id="QML36284.1"
                     /translation="MAKELSGYGPTAGESVGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGGEHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPGAGGNSDVQG
                     GYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEK
                     AKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDNLPPNVRKFDVDVEQF
                     HYLVVLDDYGNVLSITRTGVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEDPQRQ
                     NQAKKAAYDVFYSFSMNRDRIQSDVLNKAAEVISDIGNKVGDYLGNAYKSLAREIADDV
                     KNFQGKTIRSYDDAMASLNKVLSNPGFKFNRADSDALANAWRSIDAQDMANKLGNISKA
                     FKFADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASAVALGVFSATLGAYAL
                     SLGAPAIAVGIVGILLAAVVGALIDDKFADALNNEIIKPAH"
                     /name="csa"
     gene            2883..3173
                     /locus_tag="HVX42_24520"
     CDS             2883..3173
                     /locus_tag="HVX42_24520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QML36285.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            complement(3165..3443)
                     /locus_tag="HVX42_24525"
     CDS             complement(3165..3443)
                     /locus_tag="HVX42_24525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QML36286.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLASLERQKKSLR"
                     /name="hypothetical protein"
     gene            3565..3987
                     /gene="exc2"
                     /locus_tag="HVX42_24530"
     CDS             3565..3987
                     /gene="exc2"
                     /locus_tag="HVX42_24530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QML36287.1"
                     /translation="MRSAAVRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFHTQKADTIRMMHPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEEFLQSLRTTTT
                     FAGRKYTNSDMPSPEKMKLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(4307..4540)
                     /gene="mbeD"
                     /locus_tag="HVX42_24535"
     CDS             complement(4307..4540)
                     /gene="mbeD"
                     /locus_tag="HVX42_24535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QML36288.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="mbeD"
     gene            complement(4547..5065)
                     /locus_tag="HVX42_24540"
     CDS             complement(4547..5065)
                     /locus_tag="HVX42_24540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QML36289.1"
                     /translation="MSNLLQTGVEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIKGAIAQFTASTEESLKKHREGVKEAMMQHRKDMLKLAGNTGMRLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVLPYGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            complement(5043..5753)
                     /locus_tag="HVX42_24545"
                     /pseudo=""
     CDS             complement(5043..5753)
                     /locus_tag="HVX42_24545"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5743..6066)
                     /locus_tag="HVX42_24550"
     CDS             complement(5743..6066)
                     /locus_tag="HVX42_24550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QML36291.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            6131..6322
                     /locus_tag="HVX42_24555"
     CDS             6131..6322
                     /locus_tag="HVX42_24555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QML36290.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(6408..6495)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          2..171
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.491"
                     /coverage="89.0"
                     /evalue="1.2e-73"
     Typing          6518..6930
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.494"
                     /coverage="99.0"
                     /evalue="1.3500000000000002e-173"
     Typing          complement(4204..5753)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.603"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcatagctca cgctgttggt atctcggccc ggtgtaggtc gttcgctcca agctgggctg
       61 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtctcga
      121 gtccaacccg gtaagacacg acaaagcgcc actggcagca gccattggta actgaaaagt
      181 ggatttagat acgcagaact cttgaagttg aagccttata gcggctacac tgaaaggaca
      241 gcatttggta tctgtgctcc acttaagcca gctacccggt taagcagtcc ccaactgact
      301 taaccttcga ctaaaccgcc tccccaggcg gttttttcgt ttacagggca agagactacg
      361 acgatctgta aaggatctca agaagatcct ttacaggcga ccactactac cacttcccat
      421 actcactccg gtaacagtgg aagatggtga aacagcccca cctccagtat ctctgacatt
      481 gttgacttgg catgctgcaa gcagcattat tcctagtgaa atggcacaaa taataatttt
      541 tttcatatat acctttatta taatcctata ggtcaatgat gattgatgaa cattcaatag
      601 atatagacaa tagaaaagcc aataacttat tgtatttatt tatggtaatc ggagttatac
      661 ctttactctg tatactagcc gtttactata caaatccaga caatctgttc ttacacacaa
      721 tagctaccag tactgaaaac atcccatcaa taacctccgc ctacaaccca ttaatgacaa
      781 aggttatgga tatttattgt aaaacagcgc cttcccttgc tttgatatta tttatcctga
      841 catttaaaac gagaaaacta ataaaaaaaa caaaccgaaa tgctgtactt agaagttgct
      901 tattaagtcc atttgtctgt gctgtttttt tgtatttact ctgcttccga aaccttgagc
      961 taacgacagc aggaaggcca gctaggctaa tggccaacaa tgatgcaaca ctattgctat
     1021 tttatattgg cctgtactta atcatttttt ttataagcta tttcacgcta ttcacaccag
     1081 ttaccacgtt taaattatta aaagaaaggc agtaaccctg ccttcctttt acccggtatt
     1141 tattaatgag cgggtttaat gatttcgtta tttagtgcat ctgcaaactt gtcatcaatt
     1201 aatgcaccta caactgctgc gagtagaata cctacgatac cgacagcaat agcaggagcc
     1261 ccaagagata aggcatatgc ccctaatgtg gctgaaaaaa cccccagggc cacagcagaa
     1321 gcaatgccac tgagcaccca ggactccacc tccagcatca gaggtcccca gttcccggtt
     1381 tcatagccct cgatgctttt ctcccggacc ttctcaacct tcatcaccac atcggcaaat
     1441 ttgaaagcct tgctgatatt acccagttta tttgccatat cctgagcatc aatacttcgc
     1501 caggcgttcg ccagtgcatc actgtcggcc ctgttaaatt taaacccagg attagacaaa
     1561 actttattta atgacgccat tgcatcatcg tagctacgga tggttttccc ctggaaattt
     1621 ttgacatcat cagcaatttc tcttgctaac gacttataag catttccaag ataatcaccg
     1681 accttattac ctatatcaga gataacttca gcagctttat taagtacatc actctgaatc
     1741 cggtcacggt tcatactgaa agaataaaac acatcataag cagctttttt agcttgattc
     1801 tgtcgttgag gatcttcatt attaaatcct aatgcctcat atatctcttc cggggtctta
     1861 tggtcgactt tatccataat accagcctta gctttttcac tccctacata aggacgaaca
     1921 cctgttctag ttatagataa aacattacca taatcatcaa gcaccactaa atagtggaac
     1981 tgttcaacat caacatcaaa ttttctaaca ttaggaggca aattatccca cgcatcttca
     2041 gcctgttgtt ttgctttttc ctgccgctga gattcatctt tattaaaacc taacgcctca
     2101 tatatctcct caggtgtttt atgattaatt ttttccataa tacccgcctt agctttttca
     2161 ctccctacgt aaggacggac acctgttcga agaataccga tagctctacc attatcatta
     2221 agcgttacac gatatttaaa accatcgata ctcacatcaa tctctcgcgg taagttgttt
     2281 ttatcccact tacgagcaac ttcatcatta ggattttcag gaatatagcc cccctgaaca
     2341 tcactattcc cgccagcccc aggaactcca tatacaggtg tttttaccag accatttgtg
     2401 atggtaatgc gcacctgccc attatcatca acataaggcg gcacatccgg attattggtt
     2461 ttactccatc cccaccccgt ctgacttcca tgttcaccac ctccattacc agagcctgaa
     2521 ccaccacccc agtgaacccc actattacta ttattatttc caccttgctg attcagattc
     2581 gcccctgttc cccccacaga ctcaccagca gttgggccat atccacttag ttctttagcc
     2641 ataaattcct ctttgataat taaaacaata aattaaaagc aatatactgt acatataacc
     2701 actggttttg tattcagcaa aaacctacta ctcagcatta tccatgtcaa gagcatggat
     2761 tttcattttt gcaataagga tcacactatg gggaggcaga cattgagaac gtcgaaacag
     2821 aacaccggag caaatcagga tgagatataa aactgttgga tcatgaaaaa acggagaacg
     2881 atgtgagcaa atctccccgc cataaactga acaaaacaga caagcgactt ctcgacaccc
     2941 tggttgctgc cggatatgag catgacaaag cccgtgacct catccagaaa caggtttaca
     3001 cgctgacact ggctgatcag cgtcatgtgg tcagtgaaat cagtaacggt gtgaatccca
     3061 cccaggctta ctcggcggta taccaggcaa gacgcattcg cctcgcccgt aaatatctga
     3121 acggtaaaaa ggttatggaa gaaaccgggg aaaatacgcc cccatcagcg taaggatttc
     3181 ttttgccgct ccagagacgc cagtttttta cgcaaatcct ctcttttttg ggcatctctg
     3241 gtgccagcca gctctgctct caactcatcg atctgaagtt gtatcttcag tctgttactg
     3301 aacattttct gtctggcatt aacatccgca acaatgccgt tttttgtctt ctcggccttt
     3361 tgttgaaaaa cattgctgtc cgcatgactg gcaaccgaag cagaaagaac actaaaaagc
     3421 aggactggca cacatttttt cacgggatta ttcctgactc attgaccatc aagtcacatt
     3481 gggagtaaac cgacgtatga taagagatac tcttcggaga tataactccc tgagtatcaa
     3541 gattaaaaac gcaaggagat gtttatgaga tctgccgctg tcaggctgct tctgatacct
     3601 ctgataacag caacaatagc tcttacagga tgcacaccaa agaccagcct ggaacgacat
     3661 acccggcatt atgtttatgc ttcagatgat ggatttgatc ctaacttcca cacccagaaa
     3721 gcagacacca tacgtatgat gcacccgttc tttcagcagt tccgggatat ggggatgaaa
     3781 gacaaagcag ccggagtatc agcagaaacg gcacagcaac gtgtaaaaga attccactct
     3841 gaagaattcc ttcagtcact gagaaccaca accacctttg cgggcagaaa atacacgaac
     3901 agtgatatgc catcaccgga aaaaatgaag ctgctggctg acacgatttc tgcggtttat
     3961 ctcgatggct acgagggcag gcagtaaggg agacatttac cataatccct tattgtacgc
     4021 accgctgaaa cgcgttcagc gcgatcacgg cagcagacag gtaaaaatgg caacaaacca
     4081 cccgaaaaac tgccgcgatc gcgcctgata aattttaacc gcatgaatac ctatgcaacc
     4141 agatggtaca ggccacatta acccctctta atccactgaa gctgccattt ttcatggttt
     4201 caccatccca gcgaaggacc atccagcgtg cgttcctgta tttccggctg acgctcccgt
     4261 tccagggata acgcatgttc gcgttcctgt atcagccgtt cgtcccttat ctcctgttct
     4321 cgctgtataa ctggctcaag cgttctgtcc gctcgctcaa gtgctgcacc tgctgactca
     4381 actgcatgac ctgctcgttc agcatcgcgt tgtcccgttg cgtaagcgaa aacattttct
     4441 gcaattccac gaaggcgctc tcccattcgc tcagccgctg catatagtcc tgttgcagtt
     4501 gctctaatgc gttcagcaaa tgtctttcca gctccgtcac tctgtgtcac tccgtcagtt
     4561 gtacccagtc cttcccctga tagggaatca ccgttgccga tttcccgtac ggaaggacca
     4621 gaaatttcct tctcccgtcc tgcacaaact ccacgcccca tgtcttcgcg ttcagtttct
     4681 gcaatgtctc ttcctgtatc ctgatttctt ccaggttcgc ctgtatccgc ccgccaagat
     4741 accagagcgt cccgccactc gcggtaaaca ggagaaagac tatccccagc aacctcatgc
     4801 ccgtattccc tgccagcttt aacatgtcct tccggtgctg catcatcgcc tctttcaccc
     4861 cttcccggtg ttttttcagc gattcctccg tcgaggccgt aaactgggct atagcgcctt
     4921 tgattttcgt ctcgtttgag gtcacagcct cgcttacaga ttcgccgagc ttcctgaatt
     4981 cgtcgttcag cattttctct gtagattcgg ctctctcttt cagctttttc tcgaactcca
     5041 cgcccgtctg taaaagattg ctcataaaat gctcctttca gcctgatatt cttcccgccg
     5101 ttcgggtctg caatgctgat actgcttcgc gtcaccctga ccacttcaag ccctgcctct
     5161 gtgagcgcct gaatcacatc ctgacggcct cttatctccc cgacatgata aagagcgtct
     5221 atcccgcgtg tgacgctctc tgcaagcgcc tgtttcgttt ccggcaggtt atcagggagt
     5281 gtcagtatcc ggcggttctc cggggcgttg gggtcatgca gcccgtaatg gtgattcacc
     5341 agcgtctgcc aggcatcaat tctcggcctg tctgcccggt cgtagtacgg ctggaggcgt
     5401 tttccggtct gtagctccat gttcggaatg acaaaattca gctcaagccg tcccttgtcc
     5461 tggtgctcca cccacaggat gctgtactga ttcttttcga gaccgggcat cagtacgcgc
     5521 tcaaagctcg ccatcacttt ttcacgtcct cccggcggca gctccttctc cgcgaacgac
     5581 agaacgcctg acgtgtattt cttcgcaaat ggcgtggcat cgatgagttc ccggacttct
     5641 tccggattac cctgaagcac cgttgcgcct tcgcggttac gctccctccc cagcaggtaa
     5701 tcaaccggac cactgccacc accttttccc ctggcatgaa atttaactat catcccgcgt
     5761 cccctgttcc ctgacggcca gacgcagccg gcgcagctcg tccccgatgg ccatcagtgc
     5821 ggccaccacc tgaacccggt caccggaaga ccactgcccg ctgttcacct tacgggctgt
     5881 ctgattcagg ttatttccga tggcggccag ctgacgcagt aacggcggtg ccagtgtcgg
     5941 cagtttcccg gaacgggcaa ccggctcacc cagacagacc cgccgcatcc ataccgccag
     6001 ttgtttaccc tcacagcgtt ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt
     6061 gagcatcctc tcgcgtttca tcggtatcat tacccccatg aacagaaatc ccccttacac
     6121 ggaggcatca gtgactaaac aggaaaaaac tgcccttaac atggcccgct ttatcagaag
     6181 ccagacatta acgctgctgg aaaagctcaa cgaactggac gcggatgaac aggcagacat
     6241 ctgtgaatcg cttcacgacc acgccgatga gctttaccgc agctgcctcg cacgtttcgg
     6301 ggatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgcctgtg
     6361 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcaggtttta gcgggtgtcg
     6421 gggcgcagcc ctgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg
     6481 gcatcgatgc ggattgtatg aaaagtgcgc catggacggt gtgaaatgcc gcacagatgc
     6541 gtaaggagaa aatgcaactt acgacactct tccgcttcct cgctcactga ctcgctgcgc
     6601 tcggtcgttc ggctgcggcg agcggtgccg gctcactaaa aggcggaaat acggttatcc
     6661 acagaatcag gggataaggc cggaaagaac atgtgagtaa aaaggcaaag gacaaaaaaa
     6721 ggccgcgccg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6781 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6841 cggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6901 ctttctccct tcgggaagcg tggcgctttc
//
