LOCUS       CP057668                6230 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB18-C20 plasmid pRHB18-C20_4, complete
            sequence.
ACCESSION   CP057668
VERSION     CP057668.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148160
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH13 T2-C20
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 76.5080858017906x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 10:56:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,971
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,722
            CDSs (with protein)               :: 4,722
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 59 of 125
            Pseudo Genes (incomplete)         :: 54 of 125
            Pseudo Genes (internal stop)      :: 39 of 125
            Pseudo Genes (multiple problems)  :: 26 of 125
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB18-C20"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB18-C20_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(6089..6230,1..2))
                     /locus_tag="HVX43_24825"
     CDS             complement(join(6089..6230,1..2))
                     /locus_tag="HVX43_24825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QML31591.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            complement(64..324)
                     /locus_tag="HVX43_24830"
     CDS             complement(64..324)
                     /locus_tag="HVX43_24830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="QML31585.1"
                     /translation="MELKHSISDYTEAEFLEFVKKICRAEGATEEDDNKLVREFERLTE
                     HPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKPGFKQG"
                     /name="bacteriocin immunity protein"
     gene            complement(327..2054)
                     /locus_tag="HVX43_24835"
     CDS             complement(327..2054)
                     /locus_tag="HVX43_24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="QML31586.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENNP
                     WGGGSGSGVHWGGGSGNGNGGGNGNSGGGSNSSVAAVAFGFPALATPGAGTLGIAVSGE
                     ALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVTNVQV
                     STLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASFPGVP
                     SLTVSTVKGLPVSTTLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKPIYVSV
                     TDVLTPAQVKQRQDEENRLQQEWNDTHPVEVAERNYEQARAELDQANKDVARNQERQAK
                     AVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQRAQTDVN
                     NKKAAFDAAAKDKSDADAALGAALERRKQKENKEKDSKDKLDKESKRNKPGKATGKGKP
                     VGDKWLDDAGKDSGAPIPDRIADKLRDKEFKSFDDFRKKFWEEVSKDPELSKQFKDSNK
                     TNIQKGKAPFARKKDQVGGRERFELHHDKPISQDGGVYDMDNIRVTTPKRHIDIHRGK"
                     /name="colicin"
     gene            complement(2275..2808)
                     /locus_tag="HVX43_24840"
     CDS             complement(2275..2808)
                     /locus_tag="HVX43_24840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QML31587.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(2811..3737)
                     /locus_tag="HVX43_24845"
     CDS             complement(2811..3737)
                     /locus_tag="HVX43_24845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QML31588.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIEGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFETAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREYGFD
                     MRDGVSRQERTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            3914..4312
                     /locus_tag="HVX43_24850"
     CDS             3914..4312
                     /locus_tag="HVX43_24850"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QML31589.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQENERKQAVQNRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(4573..5592)
                     /locus_tag="HVX43_24855"
     CDS             complement(4573..5592)
                     /locus_tag="HVX43_24855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QML31590.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKC"
                     /name="replication initiation protein"
     BGC             1..6230
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(4818..5592)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(2815..3737)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.403"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ataacgactc cttgttgtga aagaatgcca gccattcatc cataaacaac cggcattcat
       61 attttagccc tgtttaaatc ctggcttacc gttagcagct cgccattctt taatttcttt
      121 aacaatccct tcaggactat cttccctgtc atcgcgagga taataaatca gatctgaacc
      181 atctgggtgc tcagttaatc gctcaaactc tctcactaat ttattgtcat cctcttcagt
      241 agcaccttca gctctacata ttttttttac aaactccaga aattcagcct cggtataatc
      301 actaatacta tgtttcagtt ccatttttac ttacctctat gaatatcaat atgtcgctta
      361 ggtgtggtca ctctgatatt atccatatca tagacaccac catcctgact gattggttta
      421 tcatgatgta attcaaagcg ttccctacca cctacttggt ctttcttcct tgcaaaaggt
      481 gcttttccct tttgaatgtt cgtcttatta ctgtctttaa attgcttact aagctcggga
      541 tcttttgaca cttcttccca gaatttcttc cggaaatcgt caaaactttt aaattcttta
      601 tcacgcaact tatcagcaat gcgatcagga attggcgctc ctgaatcttt acccgcatca
      661 tcaagccatt tatcaccaac tggttttcct ttacccgtcg ccttccctgg cttattccgt
      721 ttactctcct tatctaattt atccttagag tccttttctt tattttcctt ctgcttacgg
      781 cgttccagcg cagcacctaa cgcagcatca gcatcagact tatcttttgc agcagcatca
      841 aatgcagcct tcttattatt tacatccgtc tgtgcccgct gggccttaag ccctgccatt
      901 tgccacattc ggtgaccagc agccattggt tctcgggcaa atcgctcgaa ttgttttatt
      961 tcagcctttg catcagcaag agttttattc gcagcatcaa gttcactttt acgagaatta
     1021 taaacctgaa cagctttagc ctgtcgttcc tggtttctgg caacatcttt attagcctga
     1081 tccagttctg cgcgggcctg ttcataattg cgttcagcca cttctacagg atgtgtgtca
     1141 ttccattcct gctggaggcg attttcctca tcctgacgtt gttttacctg tgcaggggta
     1201 agaacatctg tcactgatac ataaatcggc ttctgcccac tttctttcgg aaaacggatc
     1261 accgcttcat gtgaaccacc accaaaggta aatcctgcag gaacggcagt ccggccttta
     1321 tcctccgtaa taccacgggg aagggttgtt gacaccggaa ggcctttgac agtgctgact
     1381 gtcagagaag ggacaccagg aaatgatgcg tgaaatacac ctggagtacg tgttggtttg
     1441 gcattcacaa caggcacact cataggcaca cctgcaacta cggcaatatg ctgtcgtgtg
     1501 tccttcacaa catctgtcac acgtttcgtc acgctgacag ttgcctggtc cagcggaagt
     1561 gtactgacct gaacattcgt cactgtttct gccggtaatg acgtcacaat ctttgacatc
     1621 atattcgggt catcttttgc tatttcagat ggcagaatgc cgtaaagcgc aataccccat
     1681 gcgctgaatt taaacggacc tttcagggct gcaaagatat cagctattgc cgcagataaa
     1741 gcctcaccag agacggcgat acccagcgtt ccggcaccag gagttgccaa agcaggaaaa
     1801 ccaaatgcca ctgctgctac ggatgaattg ctgccaccac cggaattacc attccccccg
     1861 ccattaccat tgccggagcc acctccccag tgaacaccac taccggaacc gccaccccat
     1921 ggattatttt ccgaactcca tcctgaaccg tcagaagccc caccatttcc accaagcccc
     1981 gtagggccgc cattaatgtt accacctgtg ttatgtgcgc cactgttgtg gtcgcgtcca
     2041 tctccaccgc tcataaaaat tccctctttt aagcgttaaa acaattgatt acatagttaa
     2101 tactgtacat aaaaccactg gttttatgta cagatcaaaa agtactcgcc attatttttt
     2161 ctgtcaagtc agggggggat attttttggc agttcggagg aatcactcat gaaaacggtg
     2221 aacggaacgc gaaatccttt cgcgggtcgt gaggcgttgg acaagcgaag cgcgtcagtt
     2281 tttccggctc ttacccgtga caggtttctc caccagttta tcttccagtg tcacaacttt
     2341 ctgatacacc gccgctgtgg cgttgtagtt cctgtcgagt attgttttca ttccccatcc
     2401 gagcacacag aacataacca gaaaagccac tgacacccca cccagccatt tccagctata
     2461 ccctttaagg gtgttgtatt cctgcacgat attgctgtga attgtcgcaa tatccctgtt
     2521 cagggcatcc gcgtttgcct tcatgtttgc gactgactct tccagccgct gaaaatcctg
     2581 gctgatgcgc tcacctatca gtcctgccac gtccagcttc tgcacctcat ttctgacttc
     2641 atccctgacc tgttttgcca caccgtcaga aatatcccga tatccgcgaa gtgtttccct
     2701 tgccacagcc agagaatcag caacagcttt ctgttgctcc attgcggtat gttgcagatg
     2761 ctccaccgat aacaggagcg tgagtaattt ttcttctgtg ctggccatgt tcagaatccc
     2821 atagaaaatc cacgtgacgg tgatttaacc cgttcaggtt cagttcgctc ctgtctgctc
     2881 actccgtctc tcatgtcaaa gccatactcc cgcgcaacat catgttgcag ccttctgacc
     2941 agttcatgtt gcttaaccgg agtgtccagc cattccctga tgtcatgccc gaccgttcgc
     3001 gcctgttcaa gatggcggga atagtccggt tcagaccgtt tttgcatgat gtcatgacgc
     3061 tgggcatcat caagccgtcc ggcagtttca aaacccagat gcacttcagg acgtcgttca
     3121 atgccctgtt ctttcagcgt ccggcagtca atgaagcccc tttcaaagcc atgctttctc
     3181 atatgttcgt tgtgcttcac ctcccagcgt tttcgcaatt cccgcaactg ctctttttgc
     3241 tgagtgggag tcaggcttcc tgaatccttt ttcgcacctc cccgctcggg atacctggcg
     3301 ttatagcgcc ggaaatactg ttccggtgta cgttcaatgc catcattcac acgctgactc
     3361 atcatgatat gcgcatgagg ctgttcgcct ccctcaatgc tcgctttcgg attgtgaatg
     3421 gcaaaactcc atgcatgccg ttcgccagct tcctgacgta caaaatcctg caccagttca
     3481 aggcgctgtt ccggtgtcag ttcacggggc agcgctatct ccagttcccg ataagtcgaa
     3541 ccgtttgcac gttcaaactg gtcagccgcc tgccagaaat gtgagggatt atctcgcgcc
     3601 cattccggca tgtttccgtg tgcggaatat tccaaatcct gacggtcgcg gtatttctct
     3661 ttgcgctcga tgtaatcggc gtggtttgca gcctgcccct tgccaccaaa tttaacgctg
     3721 aggtgatagg ttgccacggt ttcagccctc cgcaggagcg cactgtttct cgacgaagga
     3781 gagaaacata taagtgcgct tccttctaat tttacgaatt agcacggaag ataccccctg
     3841 cacccgccga ccgcaagcga tatactgcac gaccacaaca caccgcaaaa aaacaccagg
     3901 gagcaccacg aacatgcgca tcagcaacat cgaatggctg aagaaacgga tcggattcat
     3961 caggaagctc ggagaacaga ccgcacgaca gagacagatt attgacctgc tcgacaacga
     4021 agcaggactc acagaacagg aacgcaaact cctccacgta ctggcaacag cggaaaaaaa
     4081 cgacttacag gcacaggaga acgaacgaaa acaggcggtc caaaatcgaa tcgagggaaa
     4141 aaaacaacga cgagaacgca atcaccgact ctttctcgcg gctgggctac tgatcgaagc
     4201 aggactggta gacacaaaaa caggcgagct atgctataaa aaagacagga tattacaggc
     4261 acttaaggaa attaagtacg atctggagac atccccaaac ccagacgcgt aagtgcctcc
     4321 atccgtgttc cggttccctg gtgcttcctc gctcattcgc tcgctacgct cggtcatgag
     4381 actgcggcga gcacgaccac ctcaatcatg cggtaagccg caccattgct gggctgagag
     4441 gagaaaaccg ccggaaagcc cgctgctcaa aaagacgccg aaaggcgctg ttatctgata
     4501 taggcttgtt catgtccgcg aatttaacat cacattaaca aaaaacgggg ggtagtgttg
     4561 cttctttgtt aactaacatt taattatttg tttcttggtt agccattctc cagaattatc
     4621 catccagcgt cgaatagtcc ggctgctaac accaaccgcc actgcaatat cttccgttga
     4681 cgctcccaag atatacatat caagcgccct acgacgtttg tcgtcgttag cagtccaccg
     4741 aacagcggcg ctttttttcc ctttgtccga ctgattttct gacgaattgg ccttcccgcc
     4801 cttgcgaccg cgtgctgcct gaatttctgg cgtgtgcgta tcagccacat actgtgcgaa
     4861 agtttccggc gtgaagttcc tgtgcgtgta tttcgcaatg ctcttgccaa tagcccgaca
     4921 ttcagccagt gaaagcggaa cgggaagcga tgcattgtac atttcgacac gctggatcac
     4981 cgcttcaagc cactgtgaga acgctggcca gccctgacga atcgccctgt aagcccattt
     5041 gcgcgtcatt tcgaacaggt ggcagtttcg ccccatcccg taatgtttat cgatgctacg
     5101 acgctctgag gcgctcaaat cgagataatc tgccagttca tcgagggtat aggcgtcctc
     5161 gcgccactcc atcacctgcc attccaggtg gaacggattt ttgcaaatca ggccgctgta
     5221 gttcacatca gcacaaagtt tctcacacag cgaacgttca accgctgcgg catatttcag
     5281 cgccttaacc gaggcatcag gcgcggttct cacggcgatg ttgagtgcgt agagcaggtg
     5341 agcgtgtccg ttcacaggat ttttaacggt gatgttcggc gcgggggcgt tccggtcgct
     5401 ccagtcaatc gcagccccct cgcggtcaac gtcaaaaacc agccagaact gcgcatgagg
     5461 ctggttctgc tggatgtacc gcgcaagaag cgcacgccct ttgccggaga tgcgaaggcc
     5521 gaaagcgaga tcatccgtgt gatagggacg atggggtaaa ttttcgtcga agtattgaag
     5581 cgcggcgctc atgtgttacg attctctccg ggttatgcga cctacacgcc ctctgtattt
     5641 catgttgaat cttgccggat ctggacatga tttcaggggc gtttgttttt cagccgctta
     5701 cactacaccc aacggcaaat cctgtcgatc cttcccgaaa aaccgatcca tttaccataa
     5761 ctcccgttgt acgcacccct taaaacgccc tcagtcgctt tctgacgcaa aaaacgcaaa
     5821 aagacaagaa aacaccaggc caaaccagaa aaacgcgcca gaatcgtttt tagagggctg
     5881 cataactatg cacgaaagcg atgcgcgatc gatttcgact gttccgaagg gcgaacaggt
     5941 caaaatgaca agttttctcg ccctgggtgg aaagttctgc acggggattt tagctctcat
     6001 ctcccgcatg gaatttttgt gacatttcaa aaaaaagcgc atcagaatca gcctgacacg
     6061 ctttgttttt gtcaaagagg atttcgggtt actgcgttgc taatccggtc acttcagctg
     6121 ttgatgacgg ggatacagtc ccgccctgaa catcccggat atagtttgcc tgacatgcag
     6181 ccagaataat gactgcaaga agcaataaaa taatccctgt tatttttttc
//
