LOCUS       CP057667                6930 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB18-C20 plasmid pRHB18-C20_3, complete
            sequence.
ACCESSION   CP057667
VERSION     CP057667.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148160
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH13 T2-C20
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 76.5080858017906x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 10:56:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,971
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,722
            CDSs (with protein)               :: 4,722
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 59 of 125
            Pseudo Genes (incomplete)         :: 54 of 125
            Pseudo Genes (internal stop)      :: 39 of 125
            Pseudo Genes (multiple problems)  :: 26 of 125
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB18-C20"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB18-C20_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(1..234)
                     /gene="mbeD"
                     /locus_tag="HVX43_24770"
     CDS             complement(1..234)
                     /gene="mbeD"
                     /locus_tag="HVX43_24770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QML31575.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="mbeD"
     gene            complement(241..759)
                     /locus_tag="HVX43_24775"
     CDS             complement(241..759)
                     /locus_tag="HVX43_24775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QML31576.1"
                     /translation="MSNLLQTGVEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIKGAIAQFTASTEESLKKHREGVKEAMMQHRKDMLKLAGNTGMRLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVLPYGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            complement(737..1279)
                     /locus_tag="HVX43_24780"
                     /pseudo=""
     CDS             complement(737..1279)
                     /locus_tag="HVX43_24780"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1437..1760)
                     /locus_tag="HVX43_24785"
     CDS             complement(1437..1760)
                     /locus_tag="HVX43_24785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QML31584.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1825..2016
                     /locus_tag="HVX43_24790"
     CDS             1825..2016
                     /locus_tag="HVX43_24790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QML31577.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3015..3170)
                     /gene="csl"
                     /locus_tag="HVX43_24795"
     CDS             complement(3015..3170)
                     /gene="csl"
                     /locus_tag="HVX43_24795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="QML31578.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GSSGSL"
                     /name="csl"
     gene            3200..3739
                     /gene="csi"
                     /locus_tag="HVX43_24800"
     CDS             3200..3739
                     /gene="csi"
                     /locus_tag="HVX43_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 immunity protein"
                     /protein_id="QML31579.1"
                     /translation="MMIDEHSIDIDNRKANNLLYLFMVIGVIPLLCILAVYYTNPDNLF
                     LHTIATSTENIPSITSAYNPLMTKVMDIYCKTAPSLALILFILTFKTRKLIKKTNRNAV
                     LRSCLLSPFVCAVFLYLLCFRNLELTTAGRPARLMANNDATLLLFYIGLYLIIFFISYF
                     TLFTPVTTFKLLKERQ"
                     /name="csi"
     gene            complement(3767..5266)
                     /gene="csa"
                     /locus_tag="HVX43_24805"
     CDS             complement(3767..5266)
                     /gene="csa"
                     /locus_tag="HVX43_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /protein_id="QML31580.1"
                     /translation="MAKELSGYGPTAGESVGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGGEHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPGAGGNSDVQG
                     GYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEK
                     AKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDNLPPNVRKFDVDVEQF
                     HYLVVLDDYGNVLSITRTGVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEEPQRQ
                     NQAKKAAYDVFYSFSMNRDRIQSDVLNKAAEVISDIGNKVGDYLGNAYKSLAREIADDV
                     KNFQGKTIRSYDDAMASLNKVLSNPGFKFNRADSDALANAWRSIDAQDMANKLGNISKA
                     FKFADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASAVALGVFSATLGAYAL
                     SLGAPAIAVGIVGILLAAVVGALIDDKFADALNNEIIKPAH"
                     /name="csa"
     gene            5507..5797
                     /locus_tag="HVX43_24810"
     CDS             5507..5797
                     /locus_tag="HVX43_24810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QML31581.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            complement(5789..6067)
                     /locus_tag="HVX43_24815"
     CDS             complement(5789..6067)
                     /locus_tag="HVX43_24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QML31582.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLASLERQKKSLR"
                     /name="hypothetical protein"
     gene            6189..6611
                     /gene="exc2"
                     /locus_tag="HVX43_24820"
     CDS             6189..6611
                     /gene="exc2"
                     /locus_tag="HVX43_24820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QML31583.1"
                     /translation="MRSAAVRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFHTQKADTIRMMHPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEEFLQSLRTTTT
                     FAGRKYTNSDMPSPEKMKLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     Typing          complement(2102..2189)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          2212..2758
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.171"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(30..1447)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.812"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 ttatctcctg ttctcgctgt ataactggct caagcgttct gtccgctcgc tcaagtgctg
       61 cacctgctga ctcaactgca tgacctgctc gttcagcatc gcgttgtccc gttgcgtaag
      121 cgaaaacatt ttctgcaatt ccacgaaggc gctctcccat tcgctcagcc gctgcatata
      181 gtcctgttgc agttgctcta atgcgttcag caaatgtctt tccagctccg tcactctgtg
      241 tcactccgtc agttgtaccc agtccttccc ctgataggga atcaccgttg ccgatttccc
      301 gtacggaagg accagaaatt tccttctccc gtcctgcaca aactccacgc cccatgtctt
      361 cgcgttcagt ttctgcaatg tctcttcctg tatcctgatt tcttccaggt tcgcctgtat
      421 ccgcccgcca agataccaga gcgtcccgcc actcgcggta aacaggagaa agactatccc
      481 cagcaacctc atgcccgtat tccctgccag ctttaacatg tccttccggt gctgcatcat
      541 cgcctctttc accccttccc ggtgtttttt cagcgattcc tccgtcgagg ccgtaaactg
      601 ggctatagcg cctttgattt tcgtctcgtt tgaggtcaca gcctcgctta cagattcgcc
      661 gagcttcctg aattcgtcgt tcagcatttt ctctgtagat tcggctctct ctttcagctt
      721 tttctcgaac tccacgcccg tctgtaaaag attgctcata aaatgctcct ttcagcctga
      781 tattcttccc gccgttcggg tctgcaatgc tgatactgct tcgcgtcacc ctgaccactt
      841 caagccctgc ctctgtgagc gcctgaatca catcctgacg gcctcttatc tccccgacat
      901 gataaagagc gtctatcccg cgtgtgacgc tctctgcaag cgcctgtttc gtttccggca
      961 ggttatcagg gagtgtcagt atccggcggt tctccggggc gttggggtca tgcagcccgt
     1021 aatggtgatt caccagcgtc tgccaggcat caattctcgg cctgtctgcc cggtcgtagt
     1081 acggctggag gcgttttccg gtctgtagct ccatgttcgg aatgacaaaa ttcagctcaa
     1141 gccgtccctt gtcctggtgc tccacccaca ggatgctgta ctgattcttt tcgagaccgg
     1201 gcatcagtac gcgctcaaag ctcgccatca ctttttcacg tcctcccggc ggcagctcct
     1261 tctccgcgaa cgacagaacg cctgacgtgt atttcttcgc aaatggcgtg gcatcgatga
     1321 gttcccggac ttcttccgga ttaccctgaa gcaccgttgc gccttcgcgg ttacgctccc
     1381 tccccagcag gtaatcaacc ggaccactgc caccaccttt tcccctggca tgaaatttaa
     1441 ctatcatccc gcgtcccctg ttccctgacg gccagacgca gccggcgcag ctcgtccccg
     1501 atggccatca gtgcggccac cacctgaacc cggtcaccgg aagaccactg cccgctgttc
     1561 accttacggg ctgtctgatt caggttattt ccgatggcgg ccagctgacg cagtaacggc
     1621 ggtgccagtg tcggcagttt cccggaacgg gcaaccggct cacccagaca gacccgccgc
     1681 atccataccg ccagttgttt accctcacag cgttccagta accgggcatg ttcatcatca
     1741 gtaacccgta tcgtgagcat cctctcgcgt ttcatcggta tcattacccc catgaacaga
     1801 aatccccctt acacggaggc atcagtgact aaacaggaaa aaaccgccct taacatggcc
     1861 cgctttatca gaagccagac attaacgctg ctggaaaagc tcaacgaact ggacgcggat
     1921 gaacaggcag acatctgtga atcgcttcac gaccacgccg atgagcttta ccgcagctgc
     1981 ctcgcacgtt tcggggatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     2041 acagcttgcc tgtgagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcaggt
     2101 tttagcgggt gtcggggcgc agccctgacc cagtcacgta gcgatagcgg agtgtatact
     2161 ggcttaacta tgcggcatcg atgcggattg tatgaaaagt gcgccatgga cggtgtgaaa
     2221 tgccgcacag atgcgtaagg agaaaatgca acttacgaca ctcttccgct tcctcgctca
     2281 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt gccggctcac taaaaggcgg
     2341 aaatacggtt atccacagaa tcaggggata aggccggaaa gaacatgtga gtaaaaaggc
     2401 aaaggacaaa aaaaggccgc gccgctggcg tttttccata ggctccgccc ccctgacgag
     2461 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2521 caggcgtttc cccccggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2581 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2641 tggtatctcg gcccggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2701 gttcagcccg accgctgcgc cttatccggt aactatcgtc tcgagtccaa cccggtaaga
     2761 cacgacaaag cgccactggc agcagccatt ggtaactgaa aagtggattt agatacgcag
     2821 aactcttgaa gttgaagcct tatagcggct acactgaaag gacagcattt ggtatctgtg
     2881 ctccacttaa gccagctacc cggttaagca gtccccaact gacttaacct tcgactaaac
     2941 cgcctcccca ggcggttttt tcgtttacag ggcaagagac tacgacgatc tgtaaaggat
     3001 ctcaagaaga tcctttacag gctaccacta ctaccacttc ccatactcac tccggtaaca
     3061 gtggaagatg gtgaaacagc cccacctcca gtatctctga cattgttgac ttggcatgct
     3121 gcaagcagca ttattcctag tgaaatggca caaataataa tttttttcat atataccttt
     3181 attataatcc tataggtcaa tgatgattga tgaacattca atagatatag acaatagaaa
     3241 agccaataac ttattgtatt tatttatggt aatcggagtt atacctttac tctgtatact
     3301 agccgtttac tatacaaatc cagacaatct gttcttacac acaatagcta ccagtactga
     3361 aaacatccca tcaataacct ccgcctacaa cccattaatg acaaaggtta tggatattta
     3421 ttgtaaaaca gcgccttccc ttgctttgat attatttatc ctgacattta aaacgagaaa
     3481 actaataaaa aaaacaaacc gaaatgctgt acttagaagt tgcttattaa gtccatttgt
     3541 ctgtgctgtt tttttgtatt tactctgctt ccgaaacctt gagctaacga cagcaggaag
     3601 gccagctagg ctaatggcca acaatgatgc aacactattg ctattttata ttggcctgta
     3661 cttaatcatt ttttttataa gctatttcac gctattcaca ccagttacca cgtttaaatt
     3721 attaaaagaa aggcagtaac cctgccttcc ttttacccgg tatttattaa tgagcgggtt
     3781 taatgatttc gttatttagt gcatctgcaa acttgtcatc aattaatgca cctacaactg
     3841 ctgcgagtag aatacctacg ataccgacag caatagcagg agccccaaga gataaggcat
     3901 atgcccctaa tgtggctgaa aaaaccccca gggccacagc agaagcaatg ccactgagca
     3961 cccaggactc cacctccagc atcagaggtc cccagttccc ggtttcatag ccctcgatgc
     4021 ttttctcccg gaccttctca accttcatca ccacatcggc aaatttgaaa gccttgctga
     4081 tattacccag tttatttgcc atatcctgag catcaatact tcgccaggcg ttcgccagtg
     4141 catcactgtc ggccctgtta aatttaaacc caggattaga caaaacttta tttaatgacg
     4201 ccattgcatc atcgtagcta cggatggttt tcccctggaa atttttgaca tcatcagcaa
     4261 tttctcttgc taacgactta taagcatttc caagataatc accgacctta ttacctatat
     4321 cagagataac ttcagcagct ttattaagta catcactctg aatccggtca cggttcatac
     4381 tgaaagaata aaacacatca taagcagctt ttttagcttg attctgtcgt tgaggctctt
     4441 cattattaaa tcctaatgcc tcatatatct cttccggggt cttatggtcg actttatcca
     4501 taataccagc cttagctttt tcactcccta cataaggacg aacacctgtt ctagttatag
     4561 ataaaacatt accataatca tcaagcacca ctaaatagtg gaactgttca acatcaacat
     4621 caaattttct aacattagga ggcaaattat cccacgcatc ttcagcctgt tgttttgctt
     4681 tttcctgccg ctgagattca tctttattaa aacctaacgc ctcatatatc tcctcaggtg
     4741 ttttatgatt aattttttcc ataatacccg ccttagcttt ttcactccct acgtaaggac
     4801 ggacacctgt tcgaagaata ccgatagctc taccattatc attaagcgtt acacgatatt
     4861 taaaaccatc gatactcaca tcaatctctc gcggtaagtt gtttttatcc cacttacgag
     4921 caacttcatc attaggattt tcaggaatat agcccccctg aacatcacta ttcccgccag
     4981 ccccaggaac tccatataca ggtgttttta ccagaccatt tgtgatggta atgcgcacct
     5041 gcccattatc atcaacataa ggcggcacat ccggattatt ggttttactc catccccacc
     5101 ccgtctgact tccatgttca ccacctccat taccagagcc tgaaccacca ccccagtgaa
     5161 ccccactatt actattatta tttccacctt gctgattcag attcgcccct gttcccccca
     5221 cagactcacc agcagttggg ccatatccac ttagttcttt agccataaat tcctctttga
     5281 taattaaaac aataaattaa aagcaatata ctgtacatat aaccactggt tttgtattca
     5341 gcaaaaacct actactcagc attatccatg tcaagagcat ggattttcat ttttgcaata
     5401 aggatcacac tatggggagg cagacattga gaacgtcgaa acagaacacc ggagcaaatc
     5461 aggatgagat ataaaactgt tggatcatga aaaaacggag aacgatgtga gcaaatctcc
     5521 ccgccataaa ctgaacaaaa cagacaagcg acttctcgac accctggttg ctgccggata
     5581 tgagcatgac aaagcccgtg acctcatcca gaaacaggtt tacacgctga cactggctga
     5641 tcagcgtcat gtggtcagtg aaatcagtaa cggtgtgaat cccacccagg cttactcggc
     5701 ggtataccag gcaagacgca ttcgcctcgc ccgtaaatat ctgaacggta aaaaggttat
     5761 ggaagaaacc ggggaaaata cgcccccatc agcgtaagga tttcttttgc cgctccagag
     5821 acgccagttt tttacgcaaa tcctctcttt tttgggcatc tctggtgcca gccagctctg
     5881 ctctcaactc atcgatctga agttgtatct tcagtctgtt actgaacatt ttctgtctgg
     5941 cattaacatc cgcaacaatg ccgttttttg tcttctcggc cttttgttga aaaacattgc
     6001 tgtccgcatg actggcaacc gaagcagaaa gaacactaaa aagcaggact ggcacacatt
     6061 ttttcacggg attattcctg actcattgac catcaagtca cattgggagt aaaccgacgt
     6121 atgataagag atactcttcg gagatataac tccctgagta tcaagattaa aaacgcaagg
     6181 agatgtttat gagatctgcc gctgtcaggc tgcttctgat acctctgata acagcaacaa
     6241 tagctcttac aggatgcaca ccaaagacca gcctggaacg acatacccgg cattatgttt
     6301 atgcttcaga tgatggattt gatcctaact tccacaccca gaaagcagac accatacgta
     6361 tgatgcaccc gttctttcag cagttccggg atatggggat gaaagacaaa gcagccggag
     6421 tatcagcaga aacggcacag caacgtgtaa aagaattcca ctctgaagaa ttccttcagt
     6481 cactgagaac cacaaccacc tttgcgggca gaaaatacac gaacagtgat atgccatcac
     6541 cggaaaaaat gaagctgctg gctgacacga tttctgcggt ttatctcgat ggctacgagg
     6601 gcaggcagta agggagacat ttaccataat cccttattgt acgcaccgct gaaacgcgtt
     6661 cagcgcgatc acggcagcag acaggtaaaa atggcaacaa accacccgaa aaactgccgc
     6721 gatcgcgcct gataaatttt aaccgcatga atacctatgc aaccagatgg tacaggccac
     6781 attaacccct cttaatccac tgaagctgcc atttttcatg gtttcaccat cccagcgaag
     6841 gaccatccag cgtgcgttcc tgtatttccg gctgacgctc ccgttccagg gataacgcat
     6901 gttcgcgttc ctgtatcagc cgttcgtccc
//
