LOCUS       CP057662                6228 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB18-C23 plasmid pRHB18-C23_3, complete
            sequence.
ACCESSION   CP057662
VERSION     CP057662.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148161
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6228)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH13 T2-C23
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 177.924812801639x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 10:57:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,878
            CDSs (total)                      :: 4,754
            Genes (coding)                    :: 4,630
            CDSs (with protein)               :: 4,630
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 63 of 124
            Pseudo Genes (incomplete)         :: 54 of 124
            Pseudo Genes (internal stop)      :: 30 of 124
            Pseudo Genes (multiple problems)  :: 22 of 124
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB18-C23"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB18-C23_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(5854..6228,1..24)
                     /locus_tag="HVX44_24320"
     CDS             join(5854..6228,1..24)
                     /locus_tag="HVX44_24320"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QML26862.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQENERKQAVQNRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(285..1304)
                     /locus_tag="HVX44_24325"
     CDS             complement(285..1304)
                     /locus_tag="HVX44_24325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QML26856.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKC"
                     /name="replication initiation protein"
     gene            complement(1799..1942)
                     /locus_tag="HVX44_24330"
     CDS             complement(1799..1942)
                     /locus_tag="HVX44_24330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QML26857.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            complement(2004..2264)
                     /locus_tag="HVX44_24335"
     CDS             complement(2004..2264)
                     /locus_tag="HVX44_24335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="QML26858.1"
                     /translation="MELKHSISDYTEAEFLEFVKKICRAEGATEEDDNKLVREFERLTE
                     HPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKPGFKQG"
                     /name="bacteriocin immunity protein"
     gene            complement(2267..3994)
                     /locus_tag="HVX44_24340"
     CDS             complement(2267..3994)
                     /locus_tag="HVX44_24340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="QML26859.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENNP
                     WGGGSGSGVHWGGGSGNGNGGGNGNSGGGSNSSVAAVAFGFPALATPGAGTLGIAVSGE
                     ALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVTNVQV
                     STLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASFPGVP
                     SLTVSTVKGLPVSTTLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKPIYVSV
                     TDVLTPAQVKQRQDEENRLQQEWNDTHPVEVAERNYEQARAELDQANKDVARNQERQAK
                     AVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQRAQTDVN
                     NKKAAFDAAAKDKSDADAALGAALERRKQKENKEKDSKDKLDKESKRNKPGKATGKGKP
                     VGDKWLDDAGKDSGAPIPDRIADKLRDKEFKSFDDFRKKFWEEVSKDPELSKQFKDSNK
                     TNIQKGKAPFARKKDQVGGRERFELHHDKPISQDGGVYDMDNIRVTTPKRHIDIHRGK"
                     /name="colicin"
     gene            complement(4215..4748)
                     /locus_tag="HVX44_24345"
     CDS             complement(4215..4748)
                     /locus_tag="HVX44_24345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QML26860.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(4751..5677)
                     /locus_tag="HVX44_24350"
     CDS             complement(4751..5677)
                     /locus_tag="HVX44_24350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QML26861.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIEGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFETAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREYGFD
                     MRDGVSRQERTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     BGC             1..6228
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(530..1304)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.263"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(4755..5677)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.403"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acatccccaa acccagacgc gtaagtgcct ccatccgtgt tccggttccc tggtgcttcc
       61 tcgctcattc gctcgctacg ctcggtcatg agactgcggc gagcacgacc acctcaatca
      121 tgcggtaagc cgcaccattg ctgggctgag aggagaaaac cgccggaaag cccgctgctc
      181 aaaaagacgc cgaaaggcgc tgttatctga tataggcttg ttcatgtccg cgaatttaac
      241 atcccattaa caaaaaacgg ggggtagtgt tgcttctttg ttaactaaca tttaattatt
      301 tgtttcttgg ttagccattc tccagaatta tccatccagc gtcgaatagt ccggctgcta
      361 acaccaaccg ccactgcaat atcttccgtt gacgctccca agatatacat atcaagcgcc
      421 ctacgacgtt tgtcgtcgtt agcagtccac cgaacagcgg cgcttttttt ccctttgtcc
      481 gactgatttt ctgacgaatt ggccttcccg cccttgcgac cgcgtgctgc ctgaatttct
      541 ggcgtgtgcg tatcagccac atactgtgcg aaagtttccg gcgtgaagtt cctgtgcgtg
      601 tatttcgcaa tgctcttgcc aatagcccga cattcagcca gtgaaagcgg aacgggaagc
      661 gatgcattgt acatttcgac acgctggatc accgcttcaa gccactgtga gaacgctggc
      721 cagccctgac gaatcgccct gtaagcccat ttgcgcgtca tttcgaacag gtggcagttt
      781 cgccccatcc cgtaatgttt atcgatgcta cgacgctctg aggcgctcaa atcgagataa
      841 tcagccagtt catcgagggt ataggcttcc tcgcgccact ccatcaccag ccattccagg
      901 tggaacggat ttttgcaaat caggccgctg tagttcacat ccgcacaaag tttctcacac
      961 agcgaacgtt caaccgctgc ggcatatttc agcgccttaa ccgaggcatc aggcgcggtt
     1021 ctcacggcga tgttgagtgc gtagagcagg tgagcgtgtc cgttcacagg atttttaacg
     1081 gtgatgttcg gcgcgggagc gttccggtcg ctccagtcaa tcgcagcccc ctcgcggtca
     1141 acgtcaaaaa ccagccagaa ctgtgcatga ggctggttct gctggatata ccgtgcaaga
     1201 agcgcgcgcc ctttgccgga gatacgaagg ccgaaagcga gatcatccgt gtgataggga
     1261 cggtggggta aattttcttc gaagtattga agcgcggcgc tcatgtgtta cgattccttc
     1321 caggttatcg accgacatcg cccctgtatt ttcgtgaaag tcttggcgga gagacacgaa
     1381 tttcaggggc gtttgttttt cagtcgctta cactaccccc aacgacaaaa cctgtcgagc
     1441 cttcccgcaa atccgatcca tttaccataa ctcccgttgt acgcacccct taaaacgccc
     1501 tcagtcgctt tctgacgcaa aaaacgcaaa aagacaagaa aacaccaggc caaaccagaa
     1561 aaacgcgcca gaatcgtttt tagagggctg cataactatg cacgaaagcg atgcgcgatc
     1621 gatttcgact gttccgaagg gcgaacaggt caaaatgaca agttttctcg ccctgggtgg
     1681 aaagttctgc acggggattt tagctctcat ctcccgcatg gaatttttgt gacatttcaa
     1741 aaaaaagcgc atcagaatca gcctgacacg ctttgttttt gtcaaagagg atttcgggtt
     1801 actgcgttgc taatccggtc acttcagctg ttgatgacgg ggatacagtc ccgccctgaa
     1861 catcccggat atagtttgcc tgacatgcag ccagaataat gactgcaaga agcaataaaa
     1921 taatccctgt tatttttttc ataacgactc cttgttgtga aagaatgcca gccattcatc
     1981 cataaacaac cggcattcat attttagccc tgtttaaatc ctggcttacc gttagcagct
     2041 cgccattctt taatttcttt aacaatccct tcaggactat cttccctgtc atcgcgagga
     2101 taataaatca gatctgaacc atctgggtgc tcagttaatc gctcaaactc tctcactaat
     2161 ttattgtcat cctcttcagt agcaccttca gctctacata ttttttttac aaactccaga
     2221 aattcagcct cggtataatc actaatacta tgtttcagtt ccatttttac ttacctctat
     2281 gaatatcaat atgtcgctta ggtgtggtca ctctgatatt atccatatca tagacaccac
     2341 catcctgact gattggttta tcatgatgta attcaaagcg ttccctacca cctacttggt
     2401 ctttcttcct tgcaaaaggt gcttttccct tttgaatgtt cgtcttatta ctgtctttaa
     2461 attgcttact aagctcggga tcttttgaca cttcttccca gaatttcttc cggaaatcgt
     2521 caaaactttt aaattcttta tcacgcaact tatcagcaat gcgatcagga attggcgctc
     2581 ctgaatcttt acccgcatca tcaagccatt tatcaccaac tggttttcct ttacccgtcg
     2641 ccttccctgg cttattccgt ttactctcct tatctaattt atccttagag tccttttctt
     2701 tattttcctt ctgcttacgg cgttccagcg cagcacctaa cgcagcatca gcatcagact
     2761 tatcttttgc agcagcatca aatgcagcct tcttattatt tacatccgtc tgtgcccgct
     2821 gggccttaag ccctgccatt tgccacattc ggtgaccagc agccattggt tctcgggcaa
     2881 atcgctcgaa ttgttttatt tcagcctttg catcagcaag agttttattc gcagcatcaa
     2941 gttcactttt acgagaatta taaacctgaa cagctttagc ctgtcgttcc tggtttctgg
     3001 caacatcttt attagcctga tccagttctg cgcgggcctg ttcataattg cgttcagcca
     3061 cttctacagg atgtgtgtca ttccattcct gctggaggcg attttcctca tcctgacgtt
     3121 gttttacctg tgcaggggta agaacatctg tcactgatac ataaatcggc ttctgcccac
     3181 tttctttcgg aaaacggatc accgcttcat gtgaaccacc accaaaggta aatcctgcag
     3241 gaacggcagt ccggccttta tcctccgtaa taccacgggg aagggttgtt gacaccggaa
     3301 ggcctttgac agtgctgact gtcagagaag ggacaccagg aaatgatgcg tgaaatacac
     3361 ctggagtacg tgttggtttg gcattcacaa caggcacact cataggcaca cctgcaacta
     3421 cggcaatatg ctgtcgtgtg tccttcacaa catctgtcac acgtttcgtc acgctgacag
     3481 ttgcctggtc cagcggaagt gtactgacct gaacattcgt cactgtttct gccggtaatg
     3541 acgtcacaat ctttgacatc atattcgggt catcttttgc tatttcagat ggcagaatgc
     3601 cgtaaagcgc aataccccat gcgctgaatt taaacggacc tttcagggct gcaaagatat
     3661 cagctattgc cgcagataaa gcctcaccag agacggcgat acccagcgtt ccggcaccag
     3721 gagttgccaa agcaggaaaa ccaaatgcca ctgctgctac ggatgaattg ctgccaccac
     3781 cggaattacc attccccccg ccattaccat tgccggagcc acctccccag tgaacaccac
     3841 taccggaacc gccaccccat ggattatttt ccgaactcca tcctgaaccg tcagaagccc
     3901 caccatttcc accaagcccc gtagggccgc cattaatgtt accacctgtg ttatgtgcgc
     3961 cactgttgtg gtcgcgtcca tctccaccgc tcataaaaat tccctctttt aagcgttaaa
     4021 acaattgatt acatagttaa tactgtacat aaaaccactg gttttatgta cagatcaaaa
     4081 agtactcgcc attatttttt ctgtcaagtc agggggggat attttttggc agttcggagg
     4141 aatcactcat gaaaacggtg aacggaacgc gaaatccttt cgcgggtcgt gaggcgttgg
     4201 acaagcgaag cgcgtcagtt tttccggctc ttacccgtga caggtttctc caccagttta
     4261 tcttccagtg tcacaacttt ctgatacacc gccgctgtgg cgttgtagtt cctgtcgagt
     4321 attgttttca ttccccatcc gagcacacag aacataacca gaaaagccac tgacacccca
     4381 cccagccatt tccagctata ccctttaagg gtgttgtatt cctgcacgat attgctgtga
     4441 attgtcgcaa tatccctgtt cagggcatcc gcgtttgcct tcatgtttgc gactgactct
     4501 tccagccgct gaaaatcctg gctgatgcgc tcacctatca gtcctgccac gtccagcttc
     4561 tgcacctcat ttctgacttc atccctgacc tgttttgcca caccgtcaga aatatcccga
     4621 tatccgcgaa gtgtttccct tgccacagcc agagaatcag caacagcttt ctgttgctcc
     4681 attgcggtat gttgcagatg ctccaccgat aacaggagcg tgagtaattt ttcttctgtg
     4741 ctggccatgt tcagaatccc atagaaaatc cacgtgacgg tgatttaacc cgttcaggtt
     4801 cagttcgctc ctgtctgctc actccgtctc tcatgtcaaa gccatactcc cgcgcaacat
     4861 catgttgcag ccttctgacc agttcatgtt gcttaaccgg agtgtccagc cattccctga
     4921 tgtcatgccc gaccgttcgc gcctgttcaa gatggcggga atagtccggt tcagaccgtt
     4981 tttgcatgat gtcatgacgc tgggcatcat caagccgtcc ggcagtttca aaacccagat
     5041 gcacttcagg acgtcgttca atgccctgtt ctttcagcgt ccggcagtca atgaagcccc
     5101 tttcaaagcc atgctttctc atatgttcgt tgtgcttcac ctcccagcgt tttcgcaatt
     5161 cccgcaactg ctctttttgc tgagtgggag tcaggcttcc tgaatccttt ttcgcacctc
     5221 cccgctcggg atacctggcg ttatagcgcc ggaaatactg ttccggtgta cgttcaatgc
     5281 catcattcac acgctgactc atcatgatat gcgcatgagg ctgttcgcct ccctcaatgc
     5341 tcgctttcgg attgtgaatg gcaaaactcc atgcatgccg ttcgccagct tcctgacgta
     5401 caaaatcctg caccagttca aggcgctgtt ccggtgtcag ttcacggggc agcgctatct
     5461 ccagttcccg ataagtcgaa ccgtttgcac gttcaaactg gtcagccgcc tgccagaaat
     5521 gtgagggatt atctcgcgcc cattccggca tgtttccgtg tgcggaatat tccaaatcct
     5581 gacggtcgcg gtatttctct ttgcgctcga tgtaatcggc gtggtttgca gcctgcccct
     5641 tgccaccaaa tttaacgctg aggtgatagg ttgccacggt ttcagccctc cgcaggagcg
     5701 cactgtttct cgacgaagga gagaaacata taagtgcgct tccttctaat tttacgaatt
     5761 agcacggaag ataccccctg cacccgccga ccgcaagcga tatactgcac gaccacaaca
     5821 caccgcaaaa aaacaccagg gagcaccacg aacatgcgca tcagcaacat cgaatggctg
     5881 aagaaacgga tcggattcat caggaagctc ggagaacaga ccgcacgaca gagacagatt
     5941 attgacctgc tcgacaacga agcaggactc acagaacagg aacgcaaact cctccacgta
     6001 ctggcaacag cggaaaaaaa cgacttacag gcacaggaga acgaacgaaa acaggcggtc
     6061 caaaatcgaa tcgagggaaa aaaacaacga cgagaacgca atcaccgact ctttctcgcg
     6121 gctgggctac tgatcgaagc aggactggta gacacaaaaa caggcgagct atgctataaa
     6181 aaagacagga tattacaggc acttaaggaa attaagtacg atctggag
//
