LOCUS       CP057655                2064 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB19-C10 plasmid pRHB19-C10_6, complete
            sequence.
ACCESSION   CP057655
VERSION     CP057655.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148166
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH14 T2-C10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 118.036971671653x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 10:20:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,810
            CDSs (total)                      :: 4,691
            Genes (coding)                    :: 4,609
            CDSs (with protein)               :: 4,609
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 34 of 82
            Pseudo Genes (incomplete)         :: 33 of 82
            Pseudo Genes (internal stop)      :: 24 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB19-C10"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB19-C10_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            770..1759
                     /locus_tag="HVX49_24040"
     CDS             770..1759
                     /locus_tag="HVX49_24040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="QML17768.1"
                     /translation="MQNPKLQYLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYEHYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGEMLNRMNAAFQRMKVRKEFLPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMAPPGMLNGKSYVRHERWVELWRECLRVDYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESD"
                     /name="protein rep"
     Typing          complement(117..198)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.976"
                     /coverage="96.0"
                     /evalue="3.4000000000000004e-27"
     Typing          1763..1849
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.182"
                     /coverage="96.0"
                     /evalue="9.44e-28"
     Typing          complement(1486..1677)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.446"
                     /coverage="100.0"
                     /evalue="2.86e-91"
ORIGIN
        1 tcgacaaact ggcagtcggg gaatctcacg aagtgcgcac ttatacaaac ttcacttcgt
       61 ttcgtttgtc gtgcggtctg cgacgctcaa aaaaccgaga aacggcggga attttcggga
      121 caggatgtgt aattgtaata ccgtcacacg cgacgctatc acaattacca cctggtcagg
      181 gctgcgcccc gacaccccgc caacgcctca cggcccgcga aaaaattccg cgtactgctc
      241 accgttcccg aactcgtcaa acagaaaatc gcggggcccc gcgcctctgg cgtgggttcc
      301 gattttgtga ttgacgacgg cacggggaaa acactgggga tggtggttgg tgtcacggag
      361 tgaggccaac cacctgcaag acggtttttt gttgttcaca aaatcggggt gaaagtctgt
      421 ggatgagatg ggtgtaagtg ggtggggttg ggaggttttt ggttactgaa aatccagctc
      481 aaaattcaag caaatctcgc gcgtgatcct tgtatttata cttaagggat aactctagga
      541 ttcaaattcg cctccagccc ttgtgccgca aggcctgagg ggggatttgt cggaaaatgg
      601 ttttggagtt attctgtcgg gagtggttgc gttaaaagtg acccccgcaa cgacgggcat
      661 cgttcggggg tgtgaccacc tgcagaaact ggattcatga tgagagcaac aggtgatatg
      721 gcaatgataa cacgtcaatc gagccatcgc ccagtatggg gcggtgtgca tgcaaaaccc
      781 taaactccag tacctcactg attattcccc ctctgatgcg ccctgggacg cacacagaag
      841 cgtttctgac gacgtgggag ggatttacct gcttgcggcc gaatatgagc actacggggc
      901 tcgtatggct tcctgtggcg gtttactgcg tttcgggtgg tctaccctca aggaaaccgg
      961 agaaacgcgt ctgaggctgc gtgaggcaca tttctgccgt gtccggcact gtcctgtctg
     1021 ccagtggagg cgctccctga tgtggcaagc ccgtttctac cagtccctgc cgcgtatcgt
     1081 ggcggattat cccgatgccc gctggatgtt cctgaccctg accgtgcgca actgtgcgat
     1141 tggggaactg ggcgagatgc tgaaccggat gaatgcagct tttcagcgta tgaaggttcg
     1201 caaggagttc ctgcctgttc agggctggat ccgcacaacg gaagttaccc gcggcagcga
     1261 cggcagcgca cacccgcact tccatacctt gatgatggct cctcctggca tgctaaatgg
     1321 gaaaagttac gtcaggcatg aacgctgggt tgagctatgg cgggagtgcc tgcgggttga
     1381 ttatgacccg aatgttgatg tccgggcagt gaaaccccgc aagccgaagg atggcgagtc
     1441 cttagcctgc gcgaccgctg aactggtgcg cggcgcagtg gccgaaaccc tgaaatattc
     1501 cacgaagccg gctgacatgg tggccgatcc tgaatggttc ctcgaactga ctcgccagac
     1561 gcacaagcgc cgctttgtgg cgaccggcgg cgcactcaaa gatgtgctga aactcgatca
     1621 ggaaaccgac gccgacatgg tgatcggaga tgatatttcc gagggcgacg atgacggttc
     1681 acgcatcgcg ttcgaatgga aaaccgaggt cagaaagtac cgccgctcac cgacgaaaga
     1741 taaaactgaa tcagactagc agggtgtcgg ggcgcagccc tgaccaggtg gtaattgtga
     1801 tagcgtcgcg tgtgacggta ttacaattac acatcctgtc ccgatttttt cgccagaacg
     1861 ctggcagagc atcggcagtt ctgcgccgtt aacccctccg caggagcgca cagtttcccg
     1921 acgaaggaga gaaactacta agtgcgcatc cgcacttaac gcgcggatga tttttggcac
     1981 cgcagccacc acagctacgc aaaaatttcg gcgaacggac agcaaatcat tttgccgttt
     2041 ttgtgccgca gccgttccgc gcga
//
