LOCUS       CP057654                3751 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB19-C10 plasmid pRHB19-C10_5, complete
            sequence.
ACCESSION   CP057654
VERSION     CP057654.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148166
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH14 T2-C10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 118.036971671653x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 10:20:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,810
            CDSs (total)                      :: 4,691
            Genes (coding)                    :: 4,609
            CDSs (with protein)               :: 4,609
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 34 of 82
            Pseudo Genes (incomplete)         :: 33 of 82
            Pseudo Genes (internal stop)      :: 24 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB19-C10"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB19-C10_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(2814..3751,1..82)
                     /locus_tag="HVX49_24020"
     CDS             join(2814..3751,1..82)
                     /locus_tag="HVX49_24020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QML17767.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKC"
                     /name="replication initiation protein"
     gene            complement(343..741)
                     /locus_tag="HVX49_24025"
     CDS             complement(343..741)
                     /locus_tag="HVX49_24025"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QML17764.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            918..1844
                     /locus_tag="HVX49_24030"
     CDS             918..1844
                     /locus_tag="HVX49_24030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QML17765.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGYDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1847..2380
                     /locus_tag="HVX49_24035"
     CDS             1847..2380
                     /locus_tag="HVX49_24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QML17766.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVTLED
                     KLAEKPVTGKSRKN"
                     /name="hypothetical protein"
     Typing          2815..3589
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.263"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          919..1841
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.727"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tggtgttagc agccggacta ttcgacgctg gatggataat tctggagaat ggctaaccaa
       61 gaaacaaata attaaatgtt agttaacaaa aaagcaacac taccccccgt tttttgttaa
      121 tgggaggtta aattcgcgga catgaacaag cctatatcag ataacagcgc ctttcggcgt
      181 ctttttgagc agcgggcttt ccggcggttt tctcctctca gcccagcaat ggtgcggctt
      241 accgcatgat tgaggtggtc gtgctcgccg cagtctcatg accgagcgta gcgagcgaat
      301 gagcgaggaa gcaccaggga accggaacac ggatggaggc acttacgcgt ctgggtttgg
      361 ggatgtctcc agatcgtact taatttcctt aagtgcctgt aatatcctgt cttttttata
      421 gcatagctcg cctgtttttg tgtctaccag tcctgcttcg atcaatagcc cagccgcgag
      481 aaagagtcgg tgattgcgtt ctcgtcgttg tttttttccc tcgattcgtt tttggaccgc
      541 ctgttttcgt tcgctctcct gtgcctgtaa gtcgtttttt tccgctgttg ccagtacgtg
      601 gaggagtttg cgttcctgtt ctgtgagtcc tgcttcgttg tcgagcaggt caataatctg
      661 tctctgtcgt gcggtctgtt ctccgagctt cctgatgaat ccgatccgtt tcttcagcca
      721 ttcgatgttg ctgatgcgca tgttcgtggt gctccctggt gtttttttgc ggtgtgttgt
      781 ggtcgtgcag tatatcgctt gcggtcggcg ggtgcagggg gtatcttccg tgctaattcg
      841 taaaattaga aggaagaaca cttatatgtt tttctccttc gtcgagaaac agtgcgctcc
      901 tgcggagggc tgaaaccgtg gcaacctatc acctcagcgt taaatttggt ggcaaggggc
      961 aggctgcaaa ccacgccgat tacatcgagc gcaaagagaa ataccgcgac cgtcaggatt
     1021 tggaatattc cgcacacgga aacatgccgg aatgggcgcg agataatccc tcacatttct
     1081 ggcaggctgc tgaccagttt gaacgtgcga acggttcgac ctatcgggaa cttgaaattg
     1141 cattaccccg tgaactgaca gcggaacagc gccttgaact ggtgcaggat tttgtacgtc
     1201 aggaagctgg cgaacggcac gcctggagtt ttgccattca caatccgaaa gcgagcattg
     1261 acggcggcga acagcctcat gcgcatatca tgatgagcca gcgtgtgaat gatggcatag
     1321 aacgtactcc ggaacagtat ttccggcgct ataacgccag gtatcccgag cggggaggtg
     1381 cgaaaaagga ttccggaagc ctgactctca ctcagcaaaa agagcagttg cgggaattgc
     1441 gaaaacgctg ggaggtgaag cacaacgaac atatgagaaa gcatggcttt gaaaggggct
     1501 tcattgactg ccggacgctg aaagaacagg gcattgaacg acgtcccgaa gtgcatctgg
     1561 gttttgaagc tgccggacgg cttgatgatg cccagcgtca tgacatcatg caaaaacggt
     1621 ctgaaccgga ctattcccgc catcttgaac aggcgcgaac ggtcgggtat gacatcaggg
     1681 aatggctgga cactccggtt aagcaacatg aactggtcag aaggctgcaa catgatgttg
     1741 cgcgggagca tggctttgac atgagagaca gagtgagcag acagaaccga actgaacctg
     1801 aacgggttaa atcaccgtca cgtggatttt ctatgggatt ctgaacatgg ccagcacaga
     1861 agaaaaatta ctcacgctcc tgttatcggt ggagcatctg caacataccg caatggagca
     1921 acagaaagct gttgctgatt ctctggctgt ggcaagggaa acacttcgcg gatatcggga
     1981 tatttctgac ggtgtggcaa aacaggtcag ggatgaagtc agaaacgagg tgcagaagct
     2041 ggatgttgca gggctgatag gtgagcgcat cagccaggat tttcagcggc tggaagagtc
     2101 agtcgcaaac atgaaggcaa acgcggatgc cctgaacagg gatattgcga caattcacag
     2161 caatatcgtg caggaataca acacccttaa agggtatagc tggaaatggc tgggtggggt
     2221 gtcagtggct tttctggtta tgttctgtgt gcttggatgg ggaatgaaaa caatgctcga
     2281 caggaactac aacgccacag cggcggtgta tcagaaagtt gtgacactgg aagataaact
     2341 ggcggagaaa cctgtcacgg gcaagagccg gaaaaactga cgcgcttcgc ttgtccaacg
     2401 cctcacgacc cgcgaaaaaa ttccgcgtcc cgttcaccgt tttctgatga gaaagtttgc
     2461 tgttttgacc cgtttgccct tctgaacgat cgaaacagat cgcgcattgc tttcgtgaat
     2521 agttatgcag cctcctaaaa acaattctgc cgcgtttttc tggttcttcc tggtgtttcc
     2581 ttgtatttct gcgttttttg cgtcagaaag cgtctgagag cgttttaagg ggtgcgcaca
     2641 acgggagtta tggtaaatgg ctcggatttg cgggaaggct cgacaggttt tgtcgttggg
     2701 ggtagtgtaa gcgactgaaa aacaaacgcc cctgaaattc gtgtctctcc gccaagactt
     2761 tcacgaaaat acaggggcga tgtcggtcga taacctggaa ggaatcgtaa cacatgagcg
     2821 ccgcgcttca atacttcgaa gaaaatttac cccaccgtcc ctatcacacg gatgatctcg
     2881 ctttcggcct tcgtatctcc ggcaaagggc gcgcgcttct tgcacggtat atccagcaga
     2941 accagcctca tgcacagttc tggctggttt ttgacgttga ccgcgagggg gctgcgattg
     3001 actggagcga ccggaacgct cccgcgccga acatcaccgt taaaaatcct gtgaacggac
     3061 acgctcacct gctctacgca ctcaacatcg ccgtgagaac cgcgcctgat gcctcggtta
     3121 aggcgctgaa atatgccgca gcggttgaac gttcgctgtg tgagaaactt tgtgcggatg
     3181 tgaactacag cggcctgatt tgcaaaaatc cgttccacct ggaatggctg gtgatggagt
     3241 ggcgcgagga agcctatacc ctcgatgaac tggctgatta tctcgatttg agcgcctcag
     3301 agcgtcgtag catcgataaa cattacggga tggggcgaaa ctgccacctg ttcgaaatga
     3361 cgcgcaaatg ggcttacagg gcgattcgtc agggctggcc agcgttctca cagtggcttg
     3421 aagcggtgat ccagcgtgtc gaaatgtaca atgcatcgct tcccgttccg ctttcactgg
     3481 ctgaatgtcg ggctattggc aagagcattg cgaaatacac gcacaggaac ttcacgccgg
     3541 aaactttcgc acagtatgtg gctgatacgc acacgccaga aattcaggca gcacgcggtc
     3601 gcaagggcgg gaaggccaat tcgtcagaaa atcagtcgga caaagggaaa aaaagcgccg
     3661 ctgttcggtg gactgctaac gacgacaaac gtcgtagggc gcttgatatg tatatcttgg
     3721 gagcgtcaac ggaagatatt gcagtggcgg t
//
