LOCUS       CP057653                4726 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB19-C10 plasmid pRHB19-C10_4, complete
            sequence.
ACCESSION   CP057653
VERSION     CP057653.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148166
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH14 T2-C10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 118.036971671653x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 10:20:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,810
            CDSs (total)                      :: 4,691
            Genes (coding)                    :: 4,609
            CDSs (with protein)               :: 4,609
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 34 of 82
            Pseudo Genes (incomplete)         :: 33 of 82
            Pseudo Genes (internal stop)      :: 24 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB19-C10"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB19-C10_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(4338..4726,1..265)
                     /locus_tag="HVX49_24000"
     CDS             join(4338..4726,1..265)
                     /locus_tag="HVX49_24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C family protein"
                     /protein_id="QML17760.1"
                     /translation="MIAFSFSDIGNRCENLDLLSTINCQNDVFGMVIMDGYSCSEWNMK
                     ILSGRLDKCVFSGDLNKDIIEPCKSVPVKAAVCFLFKNNTHLHIYNAGDCRVYNKSGVL
                     LTEDNSVAWKRLKENGIDNKKIPHLVCYCPDRNYLTSFVDFCSDEIIFNHSVFDLNDEN
                     KEVVLCTDGFWEHFNDGTVIDLMKDNPESNLAKFTKNIPGNAENHTVCYVRFPS"
                     /name="protein phosphatase 2C family protein"
     gene            complement(1266..1460)
                     /locus_tag="HVX49_24005"
     CDS             complement(1266..1460)
                     /locus_tag="HVX49_24005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QML17761.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2535..3491
                     /locus_tag="HVX49_24010"
     CDS             2535..3491
                     /locus_tag="HVX49_24010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QML17762.1"
                     /translation="MYLIEEALTSWLLNEYKKPVITKEEISLQIYLFYLKKEYNGERIY
                     QIRKNKAGPNEFEKNILKLLKSGVLSRVESNDKHHAYPHLIGGFYEVFFVKGNDGSPEN
                     DIVCSTYPYGYLSYLSAMDWHNITDKIPKIIHFTTCRSEQWKNKFYHDFQKDNPDIDEA
                     KQFMPRFPRNSEVNGIKIIVTQETNYVEPMKARNSPVRVSSIGKTFMDMTRRPDYCGGE
                     EHVLESFIEHGKKYAPLIIKEVDKLGRHIDKVRVGFILDRIIGVNNKKISEWKQEAGKY
                     RGSSKVLSPKSPFSPYFDEEWFISINVDMVKSYGKRN"
                     /name="hypothetical protein"
     gene            3475..4341
                     /locus_tag="HVX49_24015"
     CDS             3475..4341
                     /locus_tag="HVX49_24015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="QML17763.1"
                     /translation="MEKEINNWIEESGGKYRLMRQAVHIILKSISIEESLNEMMVMKGG
                     TLLGVKHGSDRYTKDIDFSTEKKLSEIDLGDFFNRFNDSMDVAEAELGYGIKCKVQSHK
                     IQPNAQGTFPTVQINIAFCDRSNASAMKRLESNQSPSVINIDFSFNEKTYDFDFLSLDG
                     DDDNDSVKTYSLTDLMAEKYRSVLQQKVRERNREQDIYDINYLLGKYEFSRQDKYKILE
                     SLLKKSEGKQLDNLLNVKGIRDVEIIERSSQRYQDLSSTVKSLPLFEDAYEKVACFYES
                     LPWDIFK"
                     /name="nucleotidyl transferase AbiEii/AbiGii toxin family
                     protein"
     Typing          1101..1186
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.851"
                     /coverage="100.0"
                     /evalue="3.99e-36"
     Typing          1497..1595
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          1720..1975
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.86"
                     /coverage="89.0"
                     /evalue="1.25e-96"
     Typing          complement(529..1088)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.027"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgttattgc ccagacagaa attatctaac atcatttgtt gatttctgct ctgatgagat
       61 aattttcaat cattcagttt ttgatttaaa tgatgaaaac aaagaggttg ttttatgtac
      121 tgatgggttt tgggagcatt ttaatgatgg tactgttatt gacctcatga aagataaccc
      181 agaatctaac ttggctaaat ttactaaaaa tatcccgggt aacgcagaga atcatacggt
      241 ttgctacgtt agattccctt cttgatttcg tgttttttga agaagctatt gagatcttcg
      301 tgtcatatag ataatctctt ttctgtaaaa gaaaaaaacc gcctttgcgg ggcggttttc
      361 tcttaggttc aaagagtttg cggctcgaag aaccaaggta actggcttgg aggagcgcag
      421 tcaccaaaac ctgttctttc agtttagcct taactggcgc atgacttcaa gactaactcc
      481 tctaaatcag ttaccagtgg ctgctgccag tggcgttaag gcgtgtctta ccgggttgga
      541 ctcaagacga tagttaccgg ataaggcgca gcagtcgggc tgaacggggg gttcgtgcac
      601 acagcccagc ttggagcgaa cgacctacac cgaactgaga taccaacagc gtgagctatg
      661 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
      721 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
      781 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
      841 gagcctatgg aaaaacgcca gcggcgcggc cttttgtcct ggctttgcct tgttgctcac
      901 atgttctttc cggccttatc ccctgattct gtggataact gtgttaccgt ttttgtgtga
      961 gtcagtaccg ctcgccgcag ccgaacgacc gagcgtagcg agtcagtgag cgaggaagcg
     1021 gaaaagcgcc tggacgtgca ttttctcctt acgcatctgt gcggcatttc acacccggca
     1081 tggggcactt ttcatacaat ctgcactgat gccgcatggt taagccagta tacactccgc
     1141 tatcgctacg tgactgggtc agggcttcgc cccgacaccc gctaaaaccc cctgacgcgc
     1201 cctgacgggc ttgtctgctg ccgaaaacgc tcgcggacaa gccgtgagcg cctccgtcag
     1261 cttcgttatg ttcctgtttc actttcggtc tcaaagcgaa cctggatgct gttctggagt
     1321 tcttccgcga gttcgtgcag tcgttcgcac atggccgcct gttcgtcggc atccagagca
     1381 tccagttttt caagcagtgt caggctctgg cttttgataa atcgcgccat attcagcgca
     1441 gtttgttgct gtttgttcac cttccgttgt tctccgtcag taccgggtgt cgggtggacc
     1501 ctgaccaggt ggtaattgta agacgttgcg ggcgacggta ttacaattgc acatcctgtc
     1561 ctgttttttt aggacgtatc atggcacagc atatcaactg ataaataatc cctgtgcagt
     1621 gcagccgctg ttgcataaaa tttcgctctg cgtcgagtgt ttcttaatca ataattccgc
     1681 tgatactccg gaaaaacgcg agtctgttct catgcctgaa atgcccacac cccacgcaaa
     1741 aacaattttt tgctgatttt tctttataaa tagagagtta tgacaaatta gttcttcttg
     1801 ctctctttgc gatatttaaa aaagcggtgc cggcgcagct acaacaccgc gccgacaccg
     1861 ctttgtaggg gtggtactga ctatttttat aaaaaacatc attttatatc aggggggctg
     1921 ctagcggcgc ggtgtgtttt tttacaggac gcccctgggg gcgctgccag gggtgtctgt
     1981 tcagatatgt agcaatgagt cagggattca cgaactgaaa atgtccgaaa atgtgtctca
     2041 tgggcaccgt atgcgcccaa accgttcgtc attcattttc atgcatagct atgcagtggg
     2101 ctgaaaacga tcctgacgca ttttttcggt tttcactggg taaaacatct ctttttgcgg
     2161 tatctgcgtc agaatcgcgt tcagtgcgtt ttagcggtgc gcgtaatgcg acgttatggt
     2221 aaatgacgct gaaaaatgat cttttacgct catcgtttat gagcaacaga tgtcctaatc
     2281 atggatttga actataaccg ccgctgaatt gatcgcatga ggtgttatga aaacggtgat
     2341 ataagtaaac tttttcttga cttcctgctt attatcctat aaaataaatc ctaaggattt
     2401 attttatagg atatcaaaaa tataaaaatg gcaaatgctt gtttgttatt atttttttct
     2461 tgacaatcaa tctctaatag aatatacgtc cttaggacgt atattctatt agagactaac
     2521 cgaaaaggtt gaatatgtat cttatcgaag aagcgctaac gtcttggttg ttgaatgaat
     2581 ataaaaaacc agtcataacc aaagaagaaa tctctttgca gatttacctt ttttatttaa
     2641 aaaaagaata taatggtgag aggatatatc aaattagaaa gaacaaagca ggacctaatg
     2701 aatttgaaaa aaatatatta aaattattaa agtcaggggt tttatcaaga gttgaaagta
     2761 acgataagca tcatgcttac ccccatttaa ttggcggttt ctatgaggtc ttttttgtta
     2821 aaggtaacga tggttctccg gaaaatgata tagtgtgctc tacgtatcca tatggatact
     2881 tatcctattt atccgctatg gattggcata acatcacaga taaaataccc aagataatcc
     2941 actttacgac atgtcgttcc gaacagtgga agaataagtt ctatcatgat tttcaaaagg
     3001 ataatcctga tatagacgaa gccaaacaat tcatgcctag atttccaaga aattctgagg
     3061 tcaatggaat aaaaattatc gttacacaag aaacaaatta tgttgagcca atgaaggctc
     3121 gtaattcccc tgtcagggta tcatctatcg gtaagacctt tatggatatg accaggcggc
     3181 ctgattactg tggtggtgag gagcatgtat tggaatcatt tattgagcac ggtaaaaaat
     3241 atgcacctct gattataaag gaagtcgata agcttggccg tcatattgac aaggtaaggg
     3301 ttggattcat tttggataga attattgggg ttaataataa aaaaataagc gaatggaaac
     3361 aagaggcggg taaatacaga ggtagttcga aagtgctatc tccaaagagt ccattctcac
     3421 cttactttga tgaagaatgg tttatttcta ttaatgttga tatggttaag tcttatggaa
     3481 aaagaaatta ataattggat cgaagagtct ggtgggaagt acaggttgat gcggcaggct
     3541 gtacatataa tacttaaatc tatttctata gaggagagtt tgaatgaaat gatggttatg
     3601 aagggaggga ctcttcttgg tgttaagcat ggtagtgata gatacacaaa ggatattgat
     3661 ttttctaccg aaaaaaaact ttctgaaatt gacttggggg attttttcaa taggtttaat
     3721 gactcaatgg atgttgctga ggctgaattg ggatatggta ttaaatgtaa agttcaatct
     3781 cataaaattc aacctaatgc acaaggaaca tttccaactg tacagataaa tatagccttt
     3841 tgcgatcgtt ctaatgcgtc tgcaatgaaa aggcttgaat ctaatcaatc accttcagtg
     3901 ataaatatcg attttagctt taacgaaaaa acgtatgatt ttgacttttt atcattagat
     3961 ggtgatgatg acaatgatag cgtaaagacc tacagcttaa ctgatttgat ggctgaaaaa
     4021 tatcgatccg ttttgcaaca aaaagttaga gagagaaatc gagagcaaga tatatatgat
     4081 atcaattatc ttttaggtaa atatgagttt tctcggcaag ataagtataa aatactggag
     4141 tctcttttaa aaaagagtga aggtaaacaa ttggataatc tcttaaacgt taaaggtata
     4201 agggatgtag aaattatcga gcgttccagt cagcgatatc aggatttatc atccaccgtt
     4261 aaatcattac ctctttttga agatgcttat gaaaaagttg cctgtttcta tgaaagtctt
     4321 ccgtgggata tattcaaatg atagcgttca gttttagtga tattggtaac agatgtgaaa
     4381 acttagactt attatctacg ataaattgtc aaaatgatgt gtttggaatg gttattatgg
     4441 atgggtattc ctgctctgag tggaatatga aaattctctc tggtaggtta gataagtgcg
     4501 tgttttctgg tgatctgaat aaagatatta ttgaaccatg caagtcagtt cctgttaagg
     4561 ctgcagtatg tttccttttt aaaaataata cgcacctcca tatatataac gcaggagatt
     4621 gccgtgtata taacaaaagt ggagtccttc taacagagga taactcggtt gcctggaaaa
     4681 gattaaagga aaatggaatc gataataaaa aaatacctca cctcgt
//
