LOCUS       CP057652                6930 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB19-C10 plasmid pRHB19-C10_3, complete
            sequence.
ACCESSION   CP057652
VERSION     CP057652.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148166
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH14 T2-C10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 118.036971671653x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 10:20:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,810
            CDSs (total)                      :: 4,691
            Genes (coding)                    :: 4,609
            CDSs (with protein)               :: 4,609
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 34 of 82
            Pseudo Genes (incomplete)         :: 33 of 82
            Pseudo Genes (internal stop)      :: 24 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB19-C10"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB19-C10_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(154..477)
                     /locus_tag="HVX49_23945"
     CDS             complement(154..477)
                     /locus_tag="HVX49_23945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QML17750.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            542..733
                     /locus_tag="HVX49_23950"
     CDS             542..733
                     /locus_tag="HVX49_23950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QML17751.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1732..1887)
                     /gene="csl"
                     /locus_tag="HVX49_23955"
     CDS             complement(1732..1887)
                     /gene="csl"
                     /locus_tag="HVX49_23955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="QML17752.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GSSGSL"
                     /name="csl"
     gene            1917..2456
                     /gene="csi"
                     /locus_tag="HVX49_23960"
     CDS             1917..2456
                     /gene="csi"
                     /locus_tag="HVX49_23960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 immunity protein"
                     /protein_id="QML17753.1"
                     /translation="MMIDEHSIDIDNRKANNLLYLFMVIGVIPLLCILAVYYTNPDNLF
                     LHTIATSTENIPSITSAYNPLMTKVMDIYCKTAPSLALILFILTFKTRKLIKKTNRNAV
                     LRSCLLSPFVCAVFLYLLCFRNLELTTAGRPARLMANNDATLLLFYIGLYLIIFFISYF
                     TLFTPVTTFKLLKERQ"
                     /name="csi"
     gene            complement(2484..3983)
                     /gene="csa"
                     /locus_tag="HVX49_23965"
     CDS             complement(2484..3983)
                     /gene="csa"
                     /locus_tag="HVX49_23965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /protein_id="QML17754.1"
                     /translation="MAKELSGYGPTAGESVGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGGEHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPGAGGNSDVQG
                     GYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEK
                     AKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDNLPPNVRKFDVDVEQF
                     HYLVVLDDYGNVLSITRTGVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEEPQRQ
                     NQAKKAAYDVFYSFSMNRDRIQSDVLNKAAEVISDIGNKVGDYLGNAYKSLAREIADDV
                     KNFQGKTIRSYDDAMASLNKVLSNPGFKFNRADSDALANAWRSIDAQDMANKLGNISKA
                     FKFADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASAVALGVFSATLGAYAL
                     SLGAPAIAVGIVGILLAAVVGALIDDKFADALNNEIIKPAH"
                     /name="csa"
     gene            4224..4514
                     /locus_tag="HVX49_23970"
     CDS             4224..4514
                     /locus_tag="HVX49_23970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QML17755.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            complement(4506..4784)
                     /locus_tag="HVX49_23975"
     CDS             complement(4506..4784)
                     /locus_tag="HVX49_23975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QML17756.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLASLERQKKSLR"
                     /name="hypothetical protein"
     gene            4906..5328
                     /gene="exc2"
                     /locus_tag="HVX49_23980"
     CDS             4906..5328
                     /gene="exc2"
                     /locus_tag="HVX49_23980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QML17757.1"
                     /translation="MRSAAVRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFHTQKADTIRMMHPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEEFLQSLRTTTT
                     FAGRKYTNSDMPSPEKMKLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(5648..5881)
                     /gene="mbeD"
                     /locus_tag="HVX49_23985"
     CDS             complement(5648..5881)
                     /gene="mbeD"
                     /locus_tag="HVX49_23985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QML17758.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="mbeD"
     gene            complement(5888..6406)
                     /locus_tag="HVX49_23990"
     CDS             complement(5888..6406)
                     /locus_tag="HVX49_23990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QML17759.1"
                     /translation="MSNLLQTGVEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIKGAIAQFTASTEESLKKHREGVKEAMMQHRKDMLKLAGNTGMRLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVLPYGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            complement(6384..6923)
                     /locus_tag="HVX49_23995"
                     /pseudo=""
     CDS             complement(6384..6923)
                     /locus_tag="HVX49_23995"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(819..906)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          929..1475
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.171"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(158..510)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.305"
                     /coverage="100.0"
                     /evalue="6.13e-70"
     Typing          complement(5545..6929)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.618"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gacgtgtatt tcttcgcaaa tggcgtggca tcgatgagtt cccggacttc ttccggatta
       61 ccctgaagca ccgttgcgcc ttcgcggtta cgctccctcc ccagcaggta atcaaccgga
      121 ccactgccac caccttttcc cctggcatga aatttaacta tcatcccgcg tcccctgttc
      181 cctgacggcc agacgcagcc ggcgcagctc gtccccgatg gccatcagtg cggccaccac
      241 ctgaacccgg tcaccggaag accactgccc gctgttcacc ttacgggctg tctgattcag
      301 gttatttccg atggcggcca gctgacgcag taacggcggt gccagtgtcg gcagtttccc
      361 ggaacgggca accggctcac ccagacagac ccgccgcatc cataccgcca gttgtttacc
      421 ctcacagcgt tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct
      481 ctcgcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc
      541 agtgactaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt
      601 aacgctgctg gaaaagctca acgaactgga cgcggatgaa caggcagaca tctgtgaatc
      661 gcttcacgac cacgccgatg agctttaccg cagctgcctc gcacgtttcg gggatgacgg
      721 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgcctgt gagcggatgc
      781 cgggagcaga caagcccgtc agggcgcgtc agcaggtttt agcgggtgtc ggggcgcagc
      841 cctgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcgatg
      901 cggattgtat gaaaagtgcg ccatggacgg tgtgaaatgc cgcacagatg cgtaaggaga
      961 aaatgcaact tacgacactc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     1021 cggctgcggc gagcggtgcc ggctcactaa aaggcggaaa tacggttatc cacagaatca
     1081 ggggataagg ccggaaagaa catgtgagta aaaaggcaaa ggacaaaaaa aggccgcgcc
     1141 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1201 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ccggaagctc
     1261 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1321 ttcgggaagc gtggcgcttt ctcatagctc acgctgttgg tatctcggcc cggtgtaggt
     1381 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1441 atccggtaac tatcgtctcg agtccaaccc ggtaagacac gacaaagcgc cactggcagc
     1501 agccattggt aactgaaaag tggatttaga tacgcagaac tcttgaagtt gaagccttat
     1561 agcggctaca ctgaaaggac agcatttggt atctgtgctc cacttaagcc agctacccgg
     1621 ttaagcagtc cccaactgac ttaaccttcg actaaaccgc ctccccaggc ggttttttcg
     1681 tttacagggc aagagactac gacgatctgt aaaggatctc aagaagatcc tttacaggct
     1741 accactacta ccacttccca tactcactcc ggtaacagtg gaagatggtg aaacagcccc
     1801 acctccagta tctctgacat tgttgacttg gcatgctgca agcagcatta ttcctagtga
     1861 aatggcacaa ataataattt ttttcatata tacctttatt ataatcctat aggtcaatga
     1921 tgattgatga acattcaata gatatagaca atagaaaagc caataactta ttgtatttat
     1981 ttatggtaat cggagttata cctttactct gtatactagc cgtttactat acaaatccag
     2041 acaatctgtt cttacacaca atagctacca gtactgaaaa catcccatca ataacctccg
     2101 cctacaaccc attaatgaca aaggttatgg atatttattg taaaacagcg ccttcccttg
     2161 ctttgatatt atttatcctg acatttaaaa cgagaaaact aataaaaaaa acaaaccgaa
     2221 atgctgtact tagaagttgc ttattaagtc catttgtctg tgctgttttt ttgtatttac
     2281 tctgcttccg aaaccttgag ctaacgacag caggaaggcc agctaggcta atggccaaca
     2341 atgatgcaac actattgcta ttttatattg gcctgtactt aatcattttt tttataagct
     2401 atttcacgct attcacacca gttaccacgt ttaaattatt aaaagaaagg cagtaaccct
     2461 gccttccttt tacccggtat ttattaatga gcgggtttaa tgatttcgtt atttagtgca
     2521 tctgcaaact tgtcatcaat taatgcacct acaactgctg cgagtagaat acctacgata
     2581 ccgacagcaa tagcaggagc cccaagagat aaggcatatg cccctaatgt ggctgaaaaa
     2641 acccccaggg ccacagcaga agcaatgcca ctgagcaccc aggactccac ctccagcatc
     2701 agaggtcccc agttcccggt ttcatagccc tcgatgcttt tctcccggac cttctcaacc
     2761 ttcatcacca catcggcaaa tttgaaagcc ttgctgatat tacccagttt atttgccata
     2821 tcctgagcat caatacttcg ccaggcgttc gccagtgcat cactgtcggc cctgttaaat
     2881 ttaaacccag gattagacaa aactttattt aatgacgcca ttgcatcatc gtagctacgg
     2941 atggttttcc cctggaaatt tttgacatca tcagcaattt ctcttgctaa cgacttataa
     3001 gcatttccaa gataatcacc gaccttatta cctatatcag agataacttc agcagcttta
     3061 ttaagtacat cactctgaat ccggtcacgg ttcatactga aagaataaaa cacatcataa
     3121 gcagcttttt tagcttgatt ctgtcgttga ggctcttcat tattaaatcc taatgcctca
     3181 tatatctctt ccggggtctt atggtcgact ttatccataa taccagcctt agctttttca
     3241 ctccctacat aaggacgaac acctgttcta gttatagata aaacattacc ataatcatca
     3301 agcaccacta aatagtggaa ctgttcaaca tcaacatcaa attttctaac attaggaggc
     3361 aaattatccc acgcatcttc agcctgttgt tttgcttttt cctgccgctg agattcatct
     3421 ttattaaaac ctaacgcctc atatatctcc tcaggtgttt tatgattaat tttttccata
     3481 atacccgcct tagctttttc actccctacg taaggacgga cacctgttcg aagaataccg
     3541 atagctctac cattatcatt aagcgttaca cgatatttaa aaccatcgat actcacatca
     3601 atctctcgcg gtaagttgtt tttatcccac ttacgagcaa cttcatcatt aggattttca
     3661 ggaatatagc ccccctgaac atcactattc ccgccagccc caggaactcc atatacaggt
     3721 gtttttacca gaccatttgt gatggtaatg cgcacctgcc cattatcatc aacataaggc
     3781 ggcacatccg gattattggt tttactccat ccccaccccg tctgacttcc atgttcacca
     3841 cctccattac cagagcctga accaccaccc cagtgaaccc cactattact attattattt
     3901 ccaccttgct gattcagatt cgcccctgtc ccccccacag actcaccagc agttgggcca
     3961 tatccactta gttctttagc cataaattcc tctttgataa ttaaaacaat aaattaaaag
     4021 caatatactg tacatataac cactggtttt gtattcagca aaaacctact actcagcatt
     4081 atccatgtca agagcatgga ttttcatttt tgcaataagg atcacactat ggggaggcag
     4141 acattgagaa cgtcgaaaca gaacaccgga gcaaatcagg atgagatata aaactgttgg
     4201 atcatgaaaa aacggagaac gatgtgagca aatctccccg ccataaactg aacaaaacag
     4261 acaagcgact tctcgacacc ctggttgctg ccggatatga gcatgacaaa gcccgtgacc
     4321 tcatccagaa acaggtttac acgctgacac tggctgatca gcgtcatgtg gtcagtgaaa
     4381 tcagtaacgg tgtgaatccc acccaggctt actcggcggt ataccaggca agacgcattc
     4441 gcctcgcccg taaatatctg aacggtaaaa aggttatgga agaaaccggg gaaaatacgc
     4501 ccccatcagc gtaaggattt cttttgccgc tccagagacg ccagtttttt acgcaaatcc
     4561 tctctttttt gggcatctct ggtgccagcc agctctgctc tcaactcatc gatctgaagt
     4621 tgtatcttca gtctgttact gaacattttc tgtctggcat taacatccgc aacaatgccg
     4681 ttttttgtct tctcggcctt ttgttgaaaa acattgctgt ccgcatgact ggcaaccgaa
     4741 gcagaaagaa cactaaaaag caggactggc acacattttt tcacgggatt attcctgact
     4801 cattgaccat caagtcacat tgggagtaaa ccgacgtatg ataagagata ctcttcggag
     4861 atataactcc ctgagtatca agattaaaaa cgcaaggaga tgtttatgag atctgccgct
     4921 gtcaggctgc ttctgatacc tctgataaca gcaacaatag ctcttacagg atgcacacca
     4981 aagaccagcc tggaacgaca tacccggcat tatgtttatg cttcagatga tggatttgat
     5041 cctaacttcc acacccagaa agcagacacc atacgtatga tgcacccgtt ctttcagcag
     5101 ttccgggata tggggatgaa agacaaagca gccggagtat cagcagaaac ggcacagcaa
     5161 cgtgtaaaag aattccactc tgaagaattc cttcagtcac tgagaaccac aaccaccttt
     5221 gcgggcagaa aatacacgaa cagtgatatg ccatcaccgg aaaaaatgaa gctgctggct
     5281 gacacgattt ctgcggttta tctcgatggc tacgagggca ggcagtaagg gagacattta
     5341 ccataatccc ttattgtacg caccgctgaa acgcgttcag cgcgatcacg gcagcagaca
     5401 ggtaaaaatg gcaacaaacc acccgaaaaa ctgccgcgat cgcgcctgat aaattttaac
     5461 cgcatgaata cctatgcaac cagatggtac aggccacatt aacccctctt aatccactga
     5521 agctgccatt tttcatggtt tcaccatccc agcgaaggac catccagcgt gcgttcctgt
     5581 atttccggct gacgctcccg ttccagggat aacgcatgtt cgcgttcctg tatcagccgt
     5641 tcgtccctta tctcctgttc tcgctgtata actggctcaa gcgttctgtc cgctcgctca
     5701 agtgctgcac ctgctgactc aactgcatga cctgctcgtt cagcatcgcg ttgtcccgtt
     5761 gcgtaagcga aaacattttc tgcaattcca cgaaggcgct ctcccattcg ttcagccgct
     5821 gcatatagtc ctgttgcagt tgctctaatg cgttcagcaa atgtctttcc agctccgtca
     5881 ctctgtgtca ctccgtcagt tgtacccagt ccttcccctg atagggaatc accgttgccg
     5941 atttcccgta cggaaggacc agaaatttcc ttctcccgtc ctgcacaaac tccacgcccc
     6001 atgtcttcgc gttcagtttc tgcaatgtct cttcctgtat cctgatttct tccaggttcg
     6061 cctgtatccg cccgccaaga taccagagcg tcccgccact cgcggtaaac aggagaaaga
     6121 ctatccccag caacctcatg cccgtattcc ctgccagctt taacatgtcc ttccggtgct
     6181 gcatcatcgc ctctttcacc ccttcccggt gttttttcag cgattcctcc gtcgaggccg
     6241 taaactgggc tatagcgcct ttgattttcg tctcgtttga ggtcacagcc tcgcttacag
     6301 attcgccgag cttcctgaat tcgtcgttca gcattttctc tgtagattcg gctctctctt
     6361 tcagcttttt ctcgaactcc acgcccgtct gtaaaagatt gctcataaaa tgctcctttc
     6421 agcctgatat tcttcccgcc gttcgggtct gcaatgctga tactgcttcg cgtcaccctg
     6481 accacttcaa gccctgcctc tgtgagcgcc tgaatcacat cctgacggcc tcttatctcc
     6541 ccgacatgat aaagagcgtc tatcccgcgt gtgacgctct ctgcaagcgc ctgtttcgtt
     6601 tccggcaggt tatcagggag tgtcagtatc cggcggttct ccggggcgtt ggggtcatgc
     6661 agcccgtaat ggtgattcac cagcgtctgc caggcatcaa ttctcggcct gtctgcccgg
     6721 tcgtagtacg gctggaggcg ttttccggtc tgtagctcca tgttcggaat gacaaaattc
     6781 agctcaagcc gtcccttgtc ctggtgctcc acccacagga tgctgtactg attcttttcg
     6841 agaccgggca tcagtacgcg ctcaaagctc gccatcactt tttcacgtcc tcccggcggc
     6901 agctccttct ccgcgaacga cagaacgcct
//
