LOCUS       CP057628                6221 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB20-C17 plasmid pRHB20-C17_3, complete
            sequence.
ACCESSION   CP057628
VERSION     CP057628.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148182
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH10 T2-C17
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 123.200012659843x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 11:09:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,897
            CDSs (total)                      :: 4,776
            Genes (coding)                    :: 4,639
            CDSs (with protein)               :: 4,639
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 46 of 137
            Pseudo Genes (incomplete)         :: 75 of 137
            Pseudo Genes (internal stop)      :: 35 of 137
            Pseudo Genes (multiple problems)  :: 18 of 137
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB20-C17"
                     /isolation_source="Pooled cattle faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB20-C17_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(6045..6221,1..348))
                     /gene="cni"
                     /locus_tag="HVX65_24410"
     CDS             complement(join(6045..6221,1..348))
                     /gene="cni"
                     /locus_tag="HVX65_24410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_061352107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin N immunity protein"
                     /protein_id="QMK76281.1"
                     /translation="MDIKDRNKISKKISFSLLLLLSPFALIFFSYNNAPIPLLEKIIAY
                     LSLPGFHSLNNPPLSEAFNLYVHTAPLAAISLFIFTHKELELKPKSSPLRALKILTPFT
                     ILYISMIYCFLLTDTELTLSSKTFVLMSKNDLFLSFFYITLYIGIYIFTYLYFWFLIGT
                     YKLFTRGGILP"
                     /name="cni"
     gene            376..513
                     /locus_tag="HVX65_24415"
     CDS             376..513
                     /locus_tag="HVX65_24415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QMK76273.1"
                     /translation="MCGKILLILFFIMTLSACQVNHIRDVKGGTVAPSSSSRLTGVEIK
                     "
                     /name="colicin release lysis protein"
     gene            complement(1533..1724)
                     /locus_tag="HVX65_24420"
     CDS             complement(1533..1724)
                     /locus_tag="HVX65_24420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMK76274.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1788..2090
                     /locus_tag="HVX65_24425"
     CDS             1788..2090
                     /locus_tag="HVX65_24425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMK76282.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVRM"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2199..2717
                     /locus_tag="HVX65_24430"
     CDS             2199..2717
                     /locus_tag="HVX65_24430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QMK76275.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            2724..2957
                     /gene="mbeD"
                     /locus_tag="HVX65_24435"
     CDS             2724..2957
                     /gene="mbeD"
                     /locus_tag="HVX65_24435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMK76276.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="mbeD"
     gene            complement(3313..3483)
                     /locus_tag="HVX65_24440"
     CDS             complement(3313..3483)
                     /locus_tag="HVX65_24440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005074609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMK76277.1"
                     /translation="MSKAPIGTTASTGQKCPESGVWKVVGTPSTTAPIAKGNVMPPYNG
                     KAVTWKLTQYA"
                     /name="hypothetical protein"
     gene            complement(3677..4096)
                     /gene="exc2"
                     /locus_tag="HVX65_24445"
     CDS             complement(3677..4096)
                     /gene="exc2"
                     /locus_tag="HVX65_24445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMK76278.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQHFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(4177..4614)
                     /locus_tag="HVX65_24450"
     CDS             complement(4177..4614)
                     /locus_tag="HVX65_24450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QMK76279.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGFVSYRTSQDGRR
                     EFETSELIRAYGELKQNETPERHSAGQAENPHDQPTERILRELNELKQCLTLMLEDKQA
                     QDMDRKRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            4873..6036
                     /gene="cna"
                     /locus_tag="HVX65_24455"
     CDS             4873..6036
                     /gene="cna"
                     /locus_tag="HVX65_24455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin colicin N"
                     /protein_id="QMK76280.1"
                     /translation="MGSNGADNAHNNAFGGGKNPGIGNTSGAGSNGSASSNRGNSNGWS
                     WSNKPHKNDGFHSDGSYHITFHGDNNSKPKPGGNSGNRGNNGDGASAKVGEITITPDNS
                     KPGRYISSNPEYSLLAKLIDAESIKGTEVYTFHTRKGQYVKVTVPDSNIDKMRVDYVNW
                     KGPKYNNKLVKRFVSQFLLFRKEEKEKNEKEALLKASELVSGMGDKLGEYLGVKYKNVA
                     KEVANDIKNFHGRNIRSYNEAMASLNKVLANPKMKVNKSDKDAIVNAWKQVNAKDMANK
                     IGNLGKAFKVADLAIKVEKIREKSIEGYNTGNWGPLLLEVESWIIGGVVAGVAISLFGA
                     VLSFLPISGLAVTALGVIGIMTISYLSSFIDANRVSNINNIISSVIR"
                     /name="cna"
     Typing          1361..1448
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          complement(792..1337)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.526"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1759..2084
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.165"
                     /coverage="92.0"
                     /evalue="1.83e-63"
ORIGIN
        1 tagcaagaaa cagtatatca tggatatata aagaatagtg aaaggagtta atatctttag
       61 tgcccgcaga ggtgacgact ttggttttaa ctctaattct ttgtgtgtga atatgaataa
      121 gctgattgca gctaaagggg ctgtatgaac atagagattg aatgcttcgc ttaggggcgg
      181 gttgtttaat gaatgaaatc ctggtaggga taggtatgcg atgatttttt cgaggagtgg
      241 tattggtgca ttattataac tgaaaaatat taatgcgaat ggggaaagta agagcagaag
      301 actgaatgat attttttttg atatcttatt tctgtctttt atatccatgt tgctttcttt
      361 ggatggttga aaattatgtg tgggaaaatt ttattaatct tgttttttat catgacactt
      421 agtgcatgtc aggtcaatca catccgtgat gtgaaaggag ggactgttgc tccatcatca
      481 tcatccagac taacaggagt tgaaattaag taaaagatca aaggatcctc ttgagatcct
      541 ttttttctac gcgtaatctg ctgcctgtaa acgaaaaaac cgcctgggga ggcggtttag
      601 tcgaaggtta agtcagttgg ggactgctta accgaggtaa ctggcttcag cagagcgcag
      661 ataccaaata ctgtcctttc agtgtagccg cagttgggcc atcacttcaa gaactctgta
      721 agcatctgga taaatctcgc tctgctaatc cggttaccag tggctgctgc cagtggcgtt
      781 aaggcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
      841 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccaagccg
      901 agataccaac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
      961 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     1021 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gtgtcggttt
     1081 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccatcggcgc ggcctttttc
     1141 cggttcctgg tcttttgctc acatgttctt tccggcctta tcccctgatt ctgtggataa
     1201 ccgtattacc gtctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     1261 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tatgttctcc ttacgcatct
     1321 gtgcggcatt tcacacccgg catggcgtgc ttttcataca atccgcactg atgccgcata
     1381 gttaagccag tatacactcc gctatcgcta cgtgactggg tcagggctgc gccccgacac
     1441 ccgctaaaac ctgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgctcacaga
     1501 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atccccgaaa
     1561 cgtgcgaggc agctgcggta aagctcatcg gcgtggtcgt gaagcgattc acagatgtct
     1621 gcctgttcat ccgcgtccag ttcgttgagc ttttccagca gcgttaatgt ctggcttctg
     1681 ataaagcgcg ccatgttaag ggcggttttt tcctgtttag tcactgatgc ctccgtgtaa
     1741 gggggatttc tgttcatggg gtaatgatac cgatgaaacg cgagaggatg ctcacgatac
     1801 gggttactga tgatgaacat gccaggttac ttgaacgctg tgagggtaaa caactggcag
     1861 tatggatgcg gcgggtctgc ctgggtgagc cggttgcccg ttccgggaag ctgccgacac
     1921 tggcaccgcc gttactgcgt cagctggccg ccatcgggaa taacctgaat cagacagcga
     1981 ggaaggtgaa cagcgggcag tggtcttccg gtgaccgggt tcaggtggtg gccgcactga
     2041 tggccatcgg ggatgagctg cgccggctgc gtctggccgt caggatgtga ttcaggcgct
     2101 cacagaggcg gggcttgaag tggtcagggt gacgcgaagc agtatcagca ttgcagaccc
     2161 gaacggcggg aggaatatca gactgaaagg agcgttttat gagcaatctt ttacagacgg
     2221 gcgcggagtt cgagaaaaag ctgaaagaga gagccgaatc tacagagaaa atgctgaacg
     2281 acgagttcag gaagctcggc gagtctgtca gcgaggctgt gacctcaaac gagacgaaaa
     2341 tcagagacgc tatagccctg ttcacagcct cgacagagaa atcgctggaa aaacaccggg
     2401 aaggggtgaa agaggcgatg atgcagcaca ggaaggacgt gttaaagctg gcagggaata
     2461 cgggcatgat gttactgggg atagtctttc tcctgtttac cgcgagtggc gggacgctct
     2521 ggtatcttgg cggacggata cagacgaacc tggaagaaat caggaagcag gaagagacat
     2581 tgcagaaact gaacgcgaag acatggggcg tggagtttgt gcaggacggg aacaggaaat
     2641 tccttgtcct tccgtacggg aaatcagcgg aggtgattcc ctttcagggg aaagagtggg
     2701 tacatctgaa ggagtgacac agagtgacag agctggaaat acatttgctg aacgccttag
     2761 agcagctgca acaggactat atgcagcggc tgagcgaatg ggagagcgcc ttcgtggaat
     2821 tgcagaagat gttttcgctt acgcaacggg acaacgcgat gctgaacgag cgggtcatgc
     2881 agttgagtca gcaggtgcag cacttgagcg agcagacaga acgcttgagc cagttataca
     2941 gcgagaactg gagataagag aggaacggct gatacaggag cgcgaacatg cgttatccct
     3001 ggaacgggag cgtcagccgg aaatacagga acgcacgctg gatggtcctt cgctgggatg
     3061 gtgaaaccat gaaaaatggc agcttcagtg gattaagagg ggttaatgtg gcctgtacca
     3121 tctggttgca taggtattca tgcggttaaa atttatcagg cgcgatcgcg gcagtttttc
     3181 gggtggtttg ttgccatttt tacctgtctg ctgccgtgat cgcaccgaac gcgttttggc
     3241 ggtgcgtaca attaagggat tatggtaaat ccaagaataa cgaaaaagcc cccggtgggg
     3301 gcttttgaga tatcaagcgt attgggttaa tttccaagtt acggcttttc cgttatacgg
     3361 aggcattaca ttgcctttgg caattggtgc tgtagttgaa ggcgttccta ccactttcca
     3421 aacaccgctt tcagggcatt tttgtccggt tgaagctgtt gtaccaattg gggctttaga
     3481 catattgatt cctcttgtat gttttatccg tatgcttgtg tagcaatact tatttctggt
     3541 ttagaaatca gctttcaagc gggcttaaag gtcgtttttt cacagatgtg attgtggtca
     3601 tatttttaac aaagttgtat atgattttgg cgttgtaagt tttggttttg ttataaagga
     3661 cttatggaaa atcaacttac tgcctgccct cgtagccatc gagataaaca gccgaaatgg
     3721 tgtcagccag cagcctggat tttttcgggg tgaggtcttt gctgcggtaa tcggtacccg
     3781 caaattgtgt tgtgcctcgg agtgagttta aaaattcctg actgtgaaag tgctgaatgc
     3841 gttgctgcac atcactccgt gattttcctg tcgctttatc tttagctccc atatcccaga
     3901 actgccgaaa aaaaggcacc atcattcgtg ttgtgtcggc cttctgggtg gaaaagttag
     3961 gatcaaaacc gtcatcagaa gcatatacgt aatgccgggt atgttgttcc acgctgccct
     4021 ttggggtaca ggctgtgaga gctactgttg ctgttatcat ggcgataaga cacagtttgt
     4081 ttccggtacg tctcatgagt gtctccctac cttcaagtgt ttattctcgg tgagttatta
     4141 tccgaagact attttatcat cagtctctga cagcgttcag cgaccgaaca gcctggacca
     4201 gaatccccgt ttcttttcca gttccagtgc ctgcctgagc tgggctatct cattctgcag
     4261 ctgttcccgt tctgcttcct ggcgtttgcg atccatatcc tgtgcctgtt tgtcctcaag
     4321 catcagcgtc aggcattgtt tcagctcatt cagctcccgg agaatgcgtt ctgtcggctg
     4381 atcatgtgga ttttctgcct gtcccgcact gtgcctttct ggtgtctcat tctgctttaa
     4441 ttcgccgtat gcccggatca gttcactggt ttcaaactcc cgtcgtccgt cttgactggt
     4501 acggtaactg acaaagcctg cgcgcatatc acggtaaaac tgtgagcgcg atctgcctgt
     4561 cattttcagt gcgtcacgga gtgtgtgcca tgccataaag tgacagtgtc ccataggtgt
     4621 ctcatctcat ggtttcagta aaacataatg aggtctgaga acggtaatgt ttgtgctggt
     4681 ttttgtggta tccggcggga atagcacgtg gtgtgggtgg ctgttttttt gatcgttttc
     4741 acaaaaacga aaattcccag ggttgacagg aaaaacgcag cggcgtaatt ttaaactgta
     4801 tataaaacca gtggttatgt gtacagtatt tatttttaac ggtttgtttt aaaagtcaaa
     4861 gagggattta ttatgggtag taatggcgca gataatgcac ataacaatgc ttttggtgga
     4921 gggaaaaatc cgggcattgg taataccagt ggcgcaggaa gtaatggtag tgcatcaagt
     4981 aaccgaggaa attccaatgg atggtcatgg agtaataagc ctcataaaaa tgatggcttc
     5041 cacagtgatg gttcttacca tattacattt catggggaca ataattcaaa gcctaaacct
     5101 ggagggaata gtggaaatcg aggtaataac ggtgatggag cgagtgctaa ggttggagag
     5161 ataacaatca cacctgacaa ctcgaaacca ggtcgttata tttcgtcaaa tcctgaatat
     5221 tcattgttgg caaaattaat tgatgcggaa tcaattaaag gtacagaggt atatactttt
     5281 cacaccagaa aaggtcagta tgttaaggtt actgttccag atagtaatat tgataaaatg
     5341 agagttgatt atgtgaactg gaagggaccg aaatataaca ataaacttgt gaagaggttt
     5401 gtgagccagt ttttattatt taggaaggaa gaaaaagaaa aaaatgaaaa agaagccttg
     5461 ctaaaggcta gtgaacttgt gtctggtatg ggggataagc ttggcgagta tcttggagta
     5521 aaatataaaa atgtagctaa ggaagttgcc aatgatatta aaaacttcca tggtcgtaat
     5581 attcgtagct ataatgaagc aatggcttca cttaataaag tgttagcaaa tccaaagatg
     5641 aaagtaaaca aatctgataa ggatgccatt gtgaatgcct ggaaacaggt taatgctaag
     5701 gacatggcta ataagattgg taatcttggc aaggcattta aggttgctga tttagctata
     5761 aaggttgaga aaattaggga aaaaagcatt gagggataca atactggcaa ctggggacct
     5821 ctcctgttgg aggttgaatc atggatcatt ggtggcgttg ttgctggagt tgctattagt
     5881 ttattcgggg ctgtgttgag ttttctccca atctctggac ttgcagttac tgcgttgggg
     5941 gtaataggaa taatgacgat tagttacttg tcatctttca tagatgcaaa tcgagtttcg
     6001 aatataaata acattatatc tagtgttatt cgatgatatt agtgtcaggg gagtattcct
     6061 cccctggtaa atagcttata tgttcctata aggaaccaaa agtacaaata tgtgaatata
     6121 tatatcccaa tatatagtgt tatatagaaa aaagacaaaa acagatcgtt ttttgacatt
     6181 aatacaaatg tttttgatga caaggttagt tctgtgtcag t
//
