LOCUS       CP057624                6647 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB20-C18 plasmid pRHB20-C18_4, complete
            sequence.
ACCESSION   CP057624
VERSION     CP057624.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148183
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH10 T2-C18
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 118.267888103994x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 10:34:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,102
            CDSs (total)                      :: 4,983
            Genes (coding)                    :: 4,846
            CDSs (with protein)               :: 4,846
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 61 of 137
            Pseudo Genes (internal stop)      :: 45 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB20-C18"
                     /isolation_source="Pooled cattle faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB20-C18_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(5259..6647,1..180))
                     /gene="cea"
                     /locus_tag="HVX66_25435"
     CDS             complement(join(5259..6647,1..180))
                     /gene="cea"
                     /locus_tag="HVX66_25435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="QMK71634.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            440..877
                     /locus_tag="HVX66_25440"
     CDS             440..877
                     /locus_tag="HVX66_25440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QMK71635.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            958..1377
                     /gene="exc2"
                     /locus_tag="HVX66_25445"
     CDS             958..1377
                     /gene="exc2"
                     /locus_tag="HVX66_25445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMK71636.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(1695..1928)
                     /gene="mbeD"
                     /locus_tag="HVX66_25450"
     CDS             complement(1695..1928)
                     /gene="mbeD"
                     /locus_tag="HVX66_25450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMK71637.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="mbeD"
     gene            complement(1935..2453)
                     /locus_tag="HVX66_25455"
     CDS             complement(1935..2453)
                     /locus_tag="HVX66_25455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbeB"
                     /protein_id="QMK71638.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="mobilization protein MbeB"
     gene            complement(2431..3189)
                     /locus_tag="HVX66_25460"
                     /pseudo=""
     CDS             complement(2431..3189)
                     /locus_tag="HVX66_25460"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3131..3454)
                     /locus_tag="HVX66_25465"
     CDS             complement(3131..3454)
                     /locus_tag="HVX66_25465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMK71639.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3518..3709
                     /locus_tag="HVX66_25470"
     CDS             3518..3709
                     /locus_tag="HVX66_25470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMK71640.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4736..4873)
                     /locus_tag="HVX66_25475"
     CDS             complement(4736..4873)
                     /locus_tag="HVX66_25475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QMK71641.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            4921..5262
                     /locus_tag="HVX66_25480"
     CDS             4921..5262
                     /locus_tag="HVX66_25480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="QMK71642.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     Typing          complement(3795..3882)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          3893..4451
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.544"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1592..3141)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agcagtagac catttagcag ttgcatgaat agctgcagaa ctttcacttt tactgcctcc
       61 ttttccacct ccgccgccag agccactccc gtcaggagta ccattcaaaa gagtaataat
      121 tacctgtccc ttatcatcat aaggaacacc atctttatag tacgctaccg cggtttccat
      181 tataaaatcc tctttgactt ttaaaacaat aagttaaaaa taaatactgt acatataacc
      241 actggtttta tatacagcat aaaagctacg ccgctgcatt ttccctgtca agactgtgga
      301 cttccatttt tgtgaaaacg atcaaaaaaa cagtctttca caccacgcgc tattctcgcc
      361 cgatgccaca aaaaccagca caaacattac cgttctcaga cctcattatg ttttactgaa
      421 actatgagat gagacatcta tgggacactg tcactttatg gcatggcaca cactccggga
      481 cgcactaaaa atgacaggca gatcgcgttc acagttttac cgtgatatgc gcgcaggcct
      541 tgtcagttac cgtaccggca gggacggacg acgggagttt gaaaccagtg aactgatccg
      601 ggcatacggc gaattaaagc agaatgagac accagaaagg cacagtgagg gacatgcaga
      661 aaatccacat gatcagcaga cagaacgcat tctccgggag ctgaatgagc tgaaacaatg
      721 cctgacgctg atgcttgagg ataaacaggc acaggatatg gatcgcagac gccaggaagc
      781 agaacgggaa cagctacaaa atgagatagc ccagctcagg caggcactgg aactggaaaa
      841 gaaacgggga ttctggtcca ggttgttcgg tcgctgaacg ctgtcagaga ctgatgataa
      901 aatagtcttc ggataataac tcaccgagaa taaatacttt aaggtaggga gacactcatg
      961 agacgtaccg gaaacaaact ttgtcttatc gccatgataa cagcaacagt agctctcaca
     1021 gcctgtaccc caaagggcag cgtggaacaa catacccggc attacgtata tgcttctgat
     1081 gacggttttg atcccaactt ttccacccaa aaagccgaca caacacgaat gatggtgcct
     1141 ttttttcggc agttctggga tatgggagct aaagataaag cgacaggaaa atcacggagt
     1201 gatgtgcaac aacgcattca gcagtttcac agccaggaat ttttaaactc actccggggc
     1261 acaactcaat ttgcgggtac tgattaccgc agcaaagacc ttaccccgaa aaaatccagg
     1321 ctgctggctg acacgatttc tgcggtttat ctcgatggct acgagggcag acagtaagtg
     1381 gatttaccat aatcccttaa ttgtacgcac cgctaaaacg cgttcagcgc gatcacggca
     1441 gcagacaggt aaaaatggca acaaaccacc cgaaaaactg ccgcgatcgc gcctgataaa
     1501 ttttaaccgt atgaatacct atgcaaccag agggtacagg ccacattacc cccacttaat
     1561 ccactgaagc tgccattttt catggtttca ccatcccagc gaagggccat ccagcgtgcg
     1621 ttcctgtatt tccggctgac gctcccgttc cagggataac acatgttcgc gctcctgtat
     1681 cagccgttcc tctcttatct ccagttctcg ctgtataact ggctcaagcg ttctgtctgc
     1741 tcgctcaagt gctgcacctg ctgactcaac tgcatgaccc gctcgttcag catcgcgttg
     1801 tcccgttgcg taagcgaaaa catcttctgc aattccacga aggcgctctc ccattcgctc
     1861 agccgctgca tatagtcctg ttgcagctgc tctaaggcgt tcagcaaatg tgtttccagc
     1921 tctgtcactc tgtgtcactc cttcagatgt acccactctt tcccctgaaa gggaatcacc
     1981 tccgctgatt tcccgtacgg aaggacaagg aatttcctgt tcccgtcctg cacaaactcc
     2041 acgccccatg tcttcgcgtt cagtttctgc aatgtctctt cctgcttcct gatttcttcc
     2101 aggttcgcct gtatcctccc tccaagatac cagagcgtcc cgccactcgc ggtaaacagg
     2161 agaaagacta tccccagtaa catcatgccc gtattccctg ccagctttaa cacgtccctc
     2221 ctgtgctgca tcatcgcctc tttcacccct tcccggtgtt tttccagcga ttcctctgtc
     2281 gaggctgtga acagggctat agcgtctctg attttcgtct cgtttgatgt cacagcctcg
     2341 cttacagatt cgccgagcct cctgaactcg ttgttcagca ttttctctgt agattcggct
     2401 ctctctttca gctttttctc gaactccgcg cccgtctgca aaagattgct cataaaatgc
     2461 tcctttcagc ctgatattct tcccgccgtt cggatctgca atgctgatac tgcttcgcgt
     2521 caccctgacc acttccagcc ccgcctcagt gagcgcctga atcacatcct gacggccttt
     2581 tatctctccg gcatggtaaa gtgcatctat acctcgcgtg acgccctcag caagcgcctg
     2641 tttcgtttca ggcaggttat cagggagtgt caacgtcctg cggttctccg gggcgttcgg
     2701 gtcatgcagc ccgtaatggt gattcaccag cgtctgccag gcatcaattc taggcctgtc
     2761 tgcgcggtcg tagtacggct ggaggcgttt tccggtctgt agctccatgt tcggaatgac
     2821 aaaattcagc tcaagccgtc ccttgtcctg gtgctccacc cacaggatgc tgtactgatt
     2881 tttttcgaga ccgggcatca gtacacgctc aaagctcgcc atcacttttt cacgtcctcc
     2941 cggcggcagc tccttctccg cgaacgacag aacaccggac gtgtatttct tcgcaaatgg
     3001 cgtggcatcg atgagttccc ggacttcttc cggattaccc tgaagcaccg ttgcgccttc
     3061 gcggttacgc tccctcccca gcaggtaatc aaccggacca ctgccaccac cttttcccct
     3121 ggcatgaaat ttaactatca tcccgcgccc cctgttccct gacagccaga cgcagccggc
     3181 gcagctcatc cccgatggcc atcagtgcgg ccaccacctg aacccggtca ccggaagacc
     3241 actgcccgct gttcacctta cgggctgtct gattcaggtt atttccgatg gcggccagct
     3301 gacgcagtaa cggcggtgcc agtgtcggca gttttccgga acgggcaacc ggctccccca
     3361 ggcagacccg ccgcatccat accgccagtt gtttaccctc acagcgttca agtaaccggg
     3421 catgttcatc atcagtaacc cgtattgtga gcatcctctc gcgtttcatc ggtatcatta
     3481 ccccatgaac agaaatcccc cttacacgga ggcatcagtg actaaacagg aaaaaaccgc
     3541 ccttaacatg gcccgcttta tcagaagcca gacattaacg ctgctggaga agctcaacga
     3601 actggacgca gatgaacagg ccgatatttg tgaatcgctt cacgaccacg ccgatgagct
     3661 ttaccgcagc tgcctcgcac gtttcgggga tgacggtgaa aacctctgac acatgcagct
     3721 cccggagacg gtcacagctt gtctgtgagc ggatgccggg agctgacaag cccgtcaggg
     3781 cgcgtcagca ggttttagcg ggtgtcgggg cgcagccctg acccagtcac gtagcgatag
     3841 cggagtgtat actggcttaa ccatgcggca tcagtgcgga ttgtatgaaa agtacgccat
     3901 gccgggtgtg aaatgccgca cagatgcgta aggagaaaat gcacgtccag gcgcttttcc
     3961 gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggtactgact
     4021 cacacaaaaa cggtaacaca gttatccaca gaatcagggg ataaggccgg aaagaacatg
     4081 tgagcaaaag accaggaaca ggaagaaggc cacgtagcag gcgtttttcc ataggctccg
     4141 cccccctgac gagcatcaca aaaatagacg ctcaagtcag aggtggcgaa acccgacagg
     4201 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     4261 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     4321 tagctcacgc tgttggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4381 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4441 caacccggta aggcacgcct taacgccact ggcagcagcc actggtaacc ggattagcag
     4501 agcgatgatg gcacaaacgg tgctacagag ttcttgaagt agtggcccga ctacggctac
     4561 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4621 gttggtagct cttgatccgg caaacaaacc accgttggta gcggtggttt ttttgtttgc
     4681 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctact
     4741 gaaccgcgat ccccgtcagt ttagaagagg aggatggtgc gatggtccct ccctgaacat
     4801 cacgtatata gttagcctga catccaacaa ggaggtttat cgcgaatatt cccacaaaaa
     4861 atcttttcct cataactcga tccttataaa atgaaaagaa tatatggcga ggtttaattt
     4921 atgagcttaa gatactacat aaaaaatatt ttatttggcc tgtactgcac acttatatat
     4981 atatacctta taacaaaaaa cagcgaaggg tattatttcc ttgtgtcaga taagatgcta
     5041 tatgcaatag tgataagcac tattctatgt ccatattcaa aatatgctat tgaatacata
     5101 gcttttaact tcataaagaa agattttttc gaaagaagaa aaaacctaaa taacgccccc
     5161 gtagcaaaat taaacctatt tatgctatat aatctacttt gtttggtcct agcaatccca
     5221 tttggattgc taggactttt tatatcaata aagaataatt aaatccctaa cacctcattt
     5281 atagtattaa gtttattctt atcaatatag gagcatagaa ttcctgtaac aatagcaata
     5341 ccccaaatac ctaatgtagt tccagcaagc aagctaaaaa gtaaagcaac aacataactc
     5401 acccctgcat ctgcagcttt cttctctaat gtaagaaata gtggcttcca gtcacctgta
     5461 tccttaattt ttaggatatc agataccaca tcatatccaa aagaaacatg ccccgtaatc
     5521 ttcaagtact tggcaaactg atctaaatgt ttagcccagt catcatactt caccgatgcc
     5581 aacgcattaa aaatagcatc acgatcggct ttgctgaatt tcttatttaa aacatccttg
     5641 tatttttcaa aagcagcgag agcttcattc acattgccga ttttcttacc tttagactta
     5701 tcagcaagtt cctgtgccat tttcgaatat ttttcaccat atttttcagt cagcgtttga
     5761 taaaagctaa ctgttgcatc aacagcatcc ttaatctgtg aattaaggag attattctgt
     5821 gcttttttca aattttcttc agcttcatga acacgagcga taccggcatt acgattatta
     5881 ctgacctgag aaatagcctt ctggatctga gttatatcag catttatccg gttaatacgt
     5941 gtttctgatg ctgttacctg tttttgtttt tcttctctaa tcttaccggc cccaacccgt
     6001 cgtctggttg cttcaaaaaa aggacggttc tgaagcggat cattggctct tggtgatagt
     6061 tttttgacca gctcatccag ttctttatat ttagcggatg cctgagccag ttcatttcgt
     6121 tttccagcga gcgttttcat ttctgcatca cgggcatgga tactggagct taaacgagaa
     6181 ttgagagtct taatctctcc atccattttc accacttcag attgtgcagc agaaagtttt
     6241 ttttgggcga tctcaacagc tttagcttct tcactcaatg cagccagtcg tttctcttca
     6301 gcttcagcca gtttcaactg gcgttctgtt tcagccttct cccgttcaat ctctttacgt
     6361 cgttgttctg cttcctgaaa agccttttct gctgcttccg cttctttacg ggctttttct
     6421 tctgctttcg caaggcgcaa acgctctgct tccgcctgca tagctgcatt attagcatga
     6481 gcaagctctg ttgctgaagg cgtacgtgag gcattgtgac gaagagcctc attcacgata
     6541 tccttcaggc gctgagtcag cgcatccctg tttgcctttg ctttcgcctg tgcttccgct
     6601 gcagcttttg cccgggcagc ctgctctgcc tgtgttttct ttaattg
//
