LOCUS       CP057596                6811 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB21-C20 plasmid pRHB21-C20_3, complete
            sequence.
ACCESSION   CP057596
VERSION     CP057596.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148196
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6811)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6811)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH06 T2-C20
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.212972384581x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 10:22:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,856
            CDSs (total)                      :: 4,738
            Genes (coding)                    :: 4,626
            CDSs (with protein)               :: 4,626
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 48 of 112
            Pseudo Genes (incomplete)         :: 50 of 112
            Pseudo Genes (internal stop)      :: 40 of 112
            Pseudo Genes (multiple problems)  :: 21 of 112
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB21-C20"
                     /isolation_source="Pooled cattle faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB21-C20_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            24..548
                     /gene="cni"
                     /locus_tag="HVX79_24230"
     CDS             24..548
                     /gene="cni"
                     /locus_tag="HVX79_24230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_061352107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin N immunity protein"
                     /protein_id="QMK20928.1"
                     /translation="MDIKDRNKISKKISFSLLLLLSPFALIFFSYNNAPIPLLEKIIAY
                     LSLPGFHSLNNPPLSEAFNLYVHTAPLAAISLFIFTHKELELKPKSSPLRALKILTPFT
                     ILYISMIYCFLLTDTELTLSSKTFVLMSKNDLFLSFFYITLYIGIYIFTYLYFWFLIGT
                     YKLFTRGGILP"
                     /name="cni"
     gene            complement(557..1720)
                     /gene="cna"
                     /locus_tag="HVX79_24235"
     CDS             complement(557..1720)
                     /gene="cna"
                     /locus_tag="HVX79_24235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin colicin N"
                     /protein_id="QMK20929.1"
                     /translation="MGSNGADNAHNNAFGGGKNPGIGNTSGAGSNGSASSNRGNSNGWS
                     WSNKPHKNDGFHSDGSYHITFHGDNNSKPKPGGNSGNRGNNGDGASAKVGEITITPDNS
                     KPGRYISSNPEYSLLAKLIDAESIKGTEVYTFHTRKGQYVKVTVPDSNIDKMRVDYVNW
                     KGPKYNNKLVKRFVSQFLLFRKEEKEKNEKEALLKASELVSGMGDKLGEYLGVKYKNVA
                     KEVANDIKNFHGRNIRSYNEAMASLNKVLANPKMKVNKSDKDAIVNAWKQVNAKDMANK
                     IGNLGKAFKVADLAIKVEKIREKSIEGYNTGNWGPLLLEVESWIIGGVVAGVAISLFGA
                     VLSFLPISGLAVTALGVIGIMTISYLSSFIDANRVSNINNIISSVIR"
                     /name="cna"
     gene            1979..2416
                     /locus_tag="HVX79_24240"
     CDS             1979..2416
                     /locus_tag="HVX79_24240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QMK20930.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGFVSYRTSQDGRR
                     EFETSELIRAYGELKQNETPERHSAGQAENPHDQPTERILRELNELKQCLTLMLEDKQA
                     QDMDRKRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            2497..2916
                     /gene="exc2"
                     /locus_tag="HVX79_24245"
     CDS             2497..2916
                     /gene="exc2"
                     /locus_tag="HVX79_24245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMK20931.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQHFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            3110..3280
                     /locus_tag="HVX79_24250"
     CDS             3110..3280
                     /locus_tag="HVX79_24250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005074609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMK20932.1"
                     /translation="MSKAPIGTTASTGQKCPESGVWKVVGTPSTTAPIAKGNVMPPYNG
                     KAVTWKLTQYA"
                     /name="hypothetical protein"
     gene            complement(3636..3869)
                     /gene="mbeD"
                     /locus_tag="HVX79_24255"
     CDS             complement(3636..3869)
                     /gene="mbeD"
                     /locus_tag="HVX79_24255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMK20933.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="mbeD"
     gene            complement(3876..4394)
                     /locus_tag="HVX79_24260"
     CDS             complement(3876..4394)
                     /locus_tag="HVX79_24260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QMK20934.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(4372..5130)
                     /locus_tag="HVX79_24265"
                     /pseudo=""
     CDS             complement(4372..5130)
                     /locus_tag="HVX79_24265"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5072..5395)
                     /locus_tag="HVX79_24270"
     CDS             complement(5072..5395)
                     /locus_tag="HVX79_24270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMK20937.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5459..5650
                     /locus_tag="HVX79_24275"
     CDS             5459..5650
                     /locus_tag="HVX79_24275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMK20935.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6670..6807)
                     /locus_tag="HVX79_24280"
     CDS             complement(6670..6807)
                     /locus_tag="HVX79_24280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QMK20936.1"
                     /translation="MCGKILLILFFIMTLSACQVNHIRDVKGGTVAPSSSSRLTGVEIK
                     "
                     /name="colicin release lysis protein"
     Typing          complement(5736..5823)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          5837..6392
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.086"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3533..5082)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.992"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttcaaccatc caaagaaagc aacatggata taaaagacag aaataagata tcaaaaaaaa
       61 tatcattcag tcttctgctc ttactttccc cattcgcatt aatatttttc agttataata
      121 atgcaccaat accactcctc gaaaaaatca tcgcatacct atccctacca ggatttcatt
      181 cattaaacaa cccgccccta agcgaagcat tcaatctcta tgttcataca gcccctttag
      241 ctgcaatcag cttattcata ttcacacaca aagaattaga gttaaaacca aagtcgtcac
      301 ctctgcgggc actaaagata ttaactcctt tcactattct ttatatatcc atgatatact
      361 gtttcttgct aactgacaca gaactaacct tgtcatcaaa aacatttgta ttaatgtcaa
      421 aaaacgatct gtttttgtct tttttctata taacactata tattgggata tatatattca
      481 catatttgta cttttggttc cttataggaa catataagct atttaccagg ggaggaatac
      541 tcccctgaca ctaatatcat cgaataacac tagatataat gttatttata ttcgaaactc
      601 gatttgcatc tatgaaagat gacaagtaac taatcgtcat tattcctatt acccccaacg
      661 cagtaactgc aagtccagag attgggagaa aactcaacac agccccgaat aaactaatag
      721 caactccagc aacaacgcca ccaatgatcc atgattcaac ctccaacagg agaggtcccc
      781 agttgccagt attgtatccc tcaatacttt tttccctaat tttctcaacc tttatagcta
      841 aatcagcaac cttaaatgcc ttgccaagat taccaatctt attagccatg tccttagcat
      901 taacctgttt ccaggcattc acaatggcat ccttatcaga tttgtttact ttcatctttg
      961 gatttgctaa cactttatta agtgaagcca ttgcttcatt atagctacga atattacgac
     1021 catggaagtt tttaatatca ttggcaactt ccttagctac atttttatat tttactccaa
     1081 gatactcgcc aagcttatcc cccataccag acacaagttc actagccttt agcaaggctt
     1141 ctttttcatt tttttctttt tcttccttcc taaataataa aaactggctc acaaacctct
     1201 tcacaagttt attgttatat ttcggtccct tccagttcac ataatcaact ctcattttat
     1261 caatattact atctggaaca gtaaccttaa catactgacc ttttctggtg tgaaaagtat
     1321 atacctctgt acctttaatt gattccgcat caattaattt tgccaacaat gaatattcag
     1381 gatttgacga aatataacga cctggtttcg agttgtcagg tgtgattgtt atctctccaa
     1441 ccttagcact cgctccatca ccgttattac ctcgatttcc actattccct ccaggtttag
     1501 gctttgaatt attgtcccca tgaaatgtaa tatggtaaga accatcactg tggaagccat
     1561 catttttatg aggcttatta ctccatgacc atccattgga atttcctcgg ttacttgatg
     1621 cactaccatt acttcctgcg ccactggtat taccaatgcc cggatttttc cctccaccaa
     1681 aagcattgtt atgtgcatta tctgcgccat tactacccat aataaatccc tctttgactt
     1741 ttaaaacaaa ccgttaaaaa taaatactgt acacataacc actggtttta tatacagttt
     1801 aaaattacgc cgctgcgttt ttcctgtcaa ccctgggaat tttcgttttt gtgaaaacga
     1861 tcaaaaaaac agccacccac accacgtgct attcccgccg gataccacaa aaaccagcac
     1921 aaacattacc gttctcagac ctcattatgt tttactgaaa ccatgagatg agacacctat
     1981 gggacactgt cactttatgg catggcacac actccgtgac gcactgaaaa tgacaggcag
     2041 atcgcgctca cagttttacc gtgatatgcg cgcaggcttt gtcagttacc gtaccagtca
     2101 agacggacga cgggagtttg aaaccagtga actgatccgg gcatacggcg aattaaagca
     2161 gaatgagaca ccagaaaggc acagtgcggg acaggcagaa aatccacatg atcagccgac
     2221 agaacgcatt ctccgggagc tgaatgagct gaaacaatgc ctgacgctga tgcttgagga
     2281 caaacaggca caggatatgg atcgcaaacg ccaggaagca gaacgggaac agctgcagaa
     2341 tgagatagcc cagctcaggc aggcactgga actggaaaag aaacggggat tctggtccag
     2401 gctgttcggt cgctgaacgc tgtcagagac tgatgataaa atagtcttcg gataataact
     2461 caccgagaat aaacacttga aggtagggag acactcatga gacgtaccgg aaacaaactg
     2521 tgtcttatcg ccatgataac agcaacagta gctctcacag cctgtacccc aaagggcagc
     2581 gtggaacaac atacccggca ttacgtatat gcttctgatg acggttttga tcctaacttt
     2641 tccacccaga aggccgacac aacacgaatg atggtgcctt tttttcggca gttctgggat
     2701 atgggagcta aagataaagc gacaggaaaa tcacggagtg atgtgcagca acgcattcag
     2761 cactttcaca gtcaggaatt tttaaactca ctccgaggca caacacaatt tgcgggtacc
     2821 gattaccgca gcaaagacct caccccgaaa aaatccaggc tgctggctga caccatttcg
     2881 gctgtttatc tcgatggcta cgagggcagg cagtaagttg attttccata agtcctttat
     2941 aacaaaacca aaacttacaa cgccaaaatc atatacaact ttgttaaaaa tatgaccaca
     3001 atcacatctg tgaaaaaacg acctttaagc ccgcttgaaa gctgatttct aaaccagaaa
     3061 taagtattgc tacacaagca tacggataaa acatacaaga ggaatcaata tgtctaaagc
     3121 cccaattggt acaacagctt caaccggaca aaaatgccct gaaagcggtg tttggaaagt
     3181 ggtaggaacg ccttcaacta cagcaccaat tgccaaaggc aatgtaatgc ctccgtataa
     3241 cggaaaagcc gtaacttgga aattaaccca atacgcttga tatctcaaaa gcccccaccg
     3301 ggggcttttt cgttattctt ggatttacca taatccctta attgtacgca ccgccaaaac
     3361 gcgttcggtg cgatcacggc agcagacagg taaaaatggc aacaaaccac ccgaaaaact
     3421 gccgcgatcg cgcctgataa attttaaccg catgaatacc tatgcaacca gatggtacag
     3481 gccacattaa cccctcttaa tccactgaag ctgccatttt tcatggtttc accatcccag
     3541 cgaaggacca tccagcgtgc gttcctgtat ttccggctga cgctcccgtt ccagggataa
     3601 cgcatgttcg cgctcctgta tcagccgttc ctctcttatc tccagttctc gctgtataac
     3661 tggctcaagc gttctgtctg ctcgctcaag tgctgcacct gctgactcaa ctgcatgacc
     3721 cgctcgttca gcatcgcgtt gtcccgttgc gtaagcgaaa acatcttctg caattccacg
     3781 aaggcgctct cccattcgct cagccgctgc atatagtcct gttgcagctg ctctaaggcg
     3841 ttcagcaaat gtatttccag ctctgtcact ctgtgtcact ccttcagatg tacccactct
     3901 ttcccctgaa agggaatcac ctccgctgat ttcccgtacg gaaggacaag gaatttcctg
     3961 ttcccgtcct gcacaaactc cacgccccat gtcttcgcgt tcagtttctg caatgtctct
     4021 tcctgcttcc tgatttcttc caggttcgtc tgtatccgtc cgccaagata ccagagcgtc
     4081 ccgccactcg cggtaaacag gagaaagact atccccagta acatcatgcc cgtattccct
     4141 gccagcttta acacgtcctt cctgtgctgc atcatcgcct ctttcacccc ttcccggtgt
     4201 ttttccagcg atttctctgt cgaggctgtg aacagggcta tagcgtctct gattttcgtc
     4261 tcgtttgagg tcacagcctc gctgacagac tcgccgagct tcctgaactc gtcgttcagc
     4321 attttctctg tagattcggc tctctctttc agctttttct cgaactccgc gcccgtctgt
     4381 aaaagattgc tcataaaacg ctcctttcag tctgatattc ctcccgccgt tcgggtctgc
     4441 aatgctgata ctgcttcgcg tcaccctgac cacttcaagc cccgcctctg tgagcgcctg
     4501 aatcacatcc tgacggcctt ttatctctcc ggcatggtaa agcgcgtcta tcccgcgtgt
     4561 aacgccctca gcaatcgcct gtttcgtttc cggcagatta tcagagagtg tcagcgtcct
     4621 gcggttctcc ggggcgttcg ggtcatgcag cccgtaatgg tgattaacca gcgtctgcca
     4681 ggcatcaatt ctaggcctgt ctgcgcggtc gtagtacggc tggaggcgtt ttccggtctg
     4741 tagctccatg ttcggaatga caaaattcag ctcaagccgt cccttgtcct ggtgctccac
     4801 ccacaggatg ctgtactgat ttttttcgag accgggcatc agtacacgct caaagctcgc
     4861 catcactttt tcacgttctc ccggcggcag ctccttctcc gcgaacgaca gaacaccgga
     4921 cgtgtatttc ttcgcaaatg gcgtggcatc gatgagttcc cggacttctt ccggattacc
     4981 ctgaagcacc gttgcgcctt cgcggttacg ctccctcccc agcaggtaat caaccggacc
     5041 actgccacca ccttttcccc tggcatgaaa tttaactatc atcccgcgcc ccctgttccc
     5101 tgacggccag acgcagccgg cgcagctcat ccccgatggc catcagtgcg gccaccacct
     5161 gaacccggtc accggaagac cactgcccgc tgttcacctt cctcgctgtc tgattcaggt
     5221 tattcccgat ggcggccagc tgacgcagta acggcggtgc cagtgtcggc agcttcccgg
     5281 aacgggcaac cggctcaccc aggcagaccc gccgcatcca tactgccagt tgtttaccct
     5341 cacagcgttc aagtaacctg gcatgttcat catcagtaac ccgtatcgtg agcatcctct
     5401 cgcgtttcat cggtatcatt accccatgaa cagaaatccc ccttacacgg aggcatcagt
     5461 gactaaacag gaaaaaaccg cccttaacat ggcgcgcttt atcagaagcc agacattaac
     5521 gctgctggaa aagctcaacg aactggacgc ggatgaacag gcagacatct gtgaatcgct
     5581 tcacgaccac gccgatgagc tttaccgcag ctgcctcgca cgtttcgggg atgacggtga
     5641 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtgag cggatgccgg
     5701 gagcagacaa gcccgtcagg gcgcgtcagc aggttttagc gggtgtcggg gcgcagccct
     5761 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagtgcgg
     5821 attgtatgaa aagcacgcca tgccgggtgt gaaatgccgc acagatgcgt aaggagaaca
     5881 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     5941 ctgcggcgag cggtatcagc tcactcaaag acggtaatac ggttatccac agaatcaggg
     6001 gataaggccg gaaataacat gtgagcaaaa gaccaggaac cggaaaaagg ccgcgccgat
     6061 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaaccgac actcaagtca
     6121 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     6181 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     6241 gggaagcgtg gcgctttctc atagctcacg ctgttggtat ctcggcttgg tgtaggtcgt
     6301 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6361 cggtaactat cgtcttgagt ccaacccggt aagacacgcc ttaacgccac tggcagcagc
     6421 cactggtaac cggattagca gagcgagatt tatccagatg cttacagagt tcttgaagtg
     6481 atggcccaac tgcggctaca ctgaaaggac agtatttggt atctgcgctc tgctgaagcc
     6541 agttacctcg gttaagcagt ccccaactga cttaaccttc gactaaaccg cctccccagg
     6601 cggttttttc gtttacaggc agcagattac gcgtagaaaa aaaggatctc aagaggatcc
     6661 tttgatcttt tacttaattt caacccctgt tagtctggat gatgatgatg gagcaacagt
     6721 ccctcctttc acatcacgga tgtgattgac ctgacatgca ctaagtgtca tgataaaaaa
     6781 caagattaat aaaattttcc cacacataat t
//
