LOCUS       CP057557                6930 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB23-C04 plasmid pRHB23-C04_3, complete
            sequence.
ACCESSION   CP057557
VERSION     CP057557.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148211
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH12 T2-C04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 127.582362447169x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 09:42:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,811
            CDSs (total)                      :: 4,691
            Genes (coding)                    :: 4,575
            CDSs (with protein)               :: 4,575
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 52 of 116
            Pseudo Genes (incomplete)         :: 46 of 116
            Pseudo Genes (internal stop)      :: 37 of 116
            Pseudo Genes (multiple problems)  :: 19 of 116
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB23-C04"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB23-C04_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(6544..6930,1..1113))
                     /gene="csa"
                     /locus_tag="HVX94_23985"
     CDS             complement(join(6544..6930,1..1113))
                     /gene="csa"
                     /locus_tag="HVX94_23985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /protein_id="QMJ63574.1"
                     /translation="MAKELSGYGPTAGESVGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGGEHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPGAGGNSDVQG
                     GYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEK
                     AKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDNLPPNVRKFDVDVEQF
                     HYLVVLDDYGNVLSITRTGVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEEPQRQ
                     NQAKKAAYDVFYSFSMNRDRIQSDVLNKAAEVISDIGNKVGDYLGNAYKSLAREIADDV
                     KNFQGKTIRSYDDAMASLNKVLSNPGFKFNRADSDALANAWRSIDAQDMANKLGNISKA
                     FKFADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASAVALGVFSATLGAYAL
                     SLGAPAIAVGIVGILLAAVVGALIDDKFADALNNEIIKPAH"
                     /name="csa"
     gene            1354..1644
                     /locus_tag="HVX94_23990"
     CDS             1354..1644
                     /locus_tag="HVX94_23990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMJ63566.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            complement(1636..1914)
                     /locus_tag="HVX94_23995"
     CDS             complement(1636..1914)
                     /locus_tag="HVX94_23995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMJ63567.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLASLERQKKSLR"
                     /name="hypothetical protein"
     gene            2036..2458
                     /gene="exc2"
                     /locus_tag="HVX94_24000"
     CDS             2036..2458
                     /gene="exc2"
                     /locus_tag="HVX94_24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMJ63568.1"
                     /translation="MRSAAVRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFHTQKADTIRMMHPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEEFLQSLRTTTT
                     FAGRKYTNSDMPSPEKMKLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(2778..3011)
                     /gene="mbeD"
                     /locus_tag="HVX94_24005"
     CDS             complement(2778..3011)
                     /gene="mbeD"
                     /locus_tag="HVX94_24005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMJ63569.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="mbeD"
     gene            complement(3018..3536)
                     /locus_tag="HVX94_24010"
     CDS             complement(3018..3536)
                     /locus_tag="HVX94_24010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QMJ63570.1"
                     /translation="MSNLLQTGVEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIKGAIAQFTASTEESLKKHREGVKEAMMQHRKDMLKLAGNTGMRLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVLPYGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            complement(3514..4224)
                     /locus_tag="HVX94_24015"
                     /pseudo=""
     CDS             complement(3514..4224)
                     /locus_tag="HVX94_24015"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4214..4537)
                     /locus_tag="HVX94_24020"
     CDS             complement(4214..4537)
                     /locus_tag="HVX94_24020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMJ63575.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4602..4793
                     /locus_tag="HVX94_24025"
     CDS             4602..4793
                     /locus_tag="HVX94_24025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMJ63571.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5792..5947)
                     /gene="csl"
                     /locus_tag="HVX94_24030"
     CDS             complement(5792..5947)
                     /gene="csl"
                     /locus_tag="HVX94_24030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="QMJ63572.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GSSGSL"
                     /name="csl"
     gene            5977..6516
                     /gene="csi"
                     /locus_tag="HVX94_24035"
     CDS             5977..6516
                     /gene="csi"
                     /locus_tag="HVX94_24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 immunity protein"
                     /protein_id="QMJ63573.1"
                     /translation="MMIDEHSIDIDNRKANNLLYLFMVIGVIPLLCILAVYYTNPDNLF
                     LHTIATSTENIPSITSAYNPLMTKVMDIYCKTAPSLALILFILTFKTRKLIKKTNRNAV
                     LRSCLLSPFVCAVFLYLLCFRNLELTTAGRPARLMANNDATLLLFYIGLYLIIFFISYF
                     TLFTPVTTFKLLKERQ"
                     /name="csi"
     Typing          complement(4879..4966)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          4989..5535
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.171"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2675..4224)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.603"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cctgttaaat ttaaacccag gattagacaa aactttattt aatgacgcca ttgcatcatc
       61 gtagctacgg atggttttcc cctggaaatt tttgacatca tcagcaattt ctcttgctaa
      121 cgacttataa gcatttccaa gataatcacc gaccttatta cctatatcag agataacttc
      181 agcagcttta ttaagtacat cactctgaat ccggtcacgg ttcatactga aagaataaaa
      241 cacatcataa gcagcttttt tagcttgatt ctgtcgttga ggctcttcat tattaaatcc
      301 taatgcctca tatatctctt ccggggtctt atggtcgact ttatccataa taccagcctt
      361 agctttttca ctccctacat aaggacgaac acctgttcta gttatagata aaacattacc
      421 ataatcatca agcaccacta aatagtggaa ctgttcaaca tcaacatcaa attttctaac
      481 attaggaggc aaattatccc acgcatcttc agcctgttgt tttgcttttt cctgccgctg
      541 agattcatct ttattaaaac ctaacgcctc atatatctcc tcaggtgttt tatgattaat
      601 tttttccata atacccgcct tagctttttc actccctacg taaggacgga cacctgttcg
      661 aagaataccg atagctctac cattatcatt aagcgttaca cgatatttaa aaccatcgat
      721 actcacatca atctctcgcg gtaagttgtt tttatcccac ttacgagcaa cttcatcatt
      781 aggattttca ggaatatagc ccccctgaac atcactattc ccgccagccc caggaactcc
      841 atatacaggt gtttttacca gaccatttgt gatggtaatg cgcacctgcc cattatcatc
      901 aacataaggc ggcacatccg gattattggt tttactccat ccccaccccg tctgacttcc
      961 atgttcacca cctccattac cagagcctga accaccaccc cagtgaaccc cactattact
     1021 attattattt ccaccttgct gattcagatt cgcccctgtt ccccccacag actcaccagc
     1081 agttgggcca tatccactta gttctttagc cataaattcc tctttgataa ttaaaacaat
     1141 aaattaaaag caatatactg tacatataac cactggtttt gtattcagca aaaacctact
     1201 actcagcatt atccatgtca agagcatgga ttttcatttt tgcaataagg atcacactat
     1261 ggggaggcag acattgagaa cgtcgaaaca gaacaccgga gcaaatcagg atgagatata
     1321 aaactgttgg atcatgaaaa aacggagaac gatgtgagca aatctccccg ccataaactg
     1381 aacaaaacag acaagcgact tctcgacacc ctggttgctg ccggatatga gcatgacaaa
     1441 gcccgtgacc tcatccagaa acaggtttac acgctgacac tggctgatca gcgtcatgtg
     1501 gtcagtgaaa tcagtaacgg tgtgaatccc acccaggctt actcggcggt ataccaggca
     1561 agacgcattc gcctcgcccg taaatatctg aacggtaaaa aggttatgga agaaaccggg
     1621 gaaaatacgc ccccatcagc gtaaggattt cttttgccgc tccagagacg ccagtttttt
     1681 acgcaaatcc tctctttttt gggcatctct ggtgccagcc agctctgctc tcaactcatc
     1741 gatctgaagt tgtatcttca gtctgttact gaacattttc tgtctggcat taacatccgc
     1801 aacaatgccg ttttttgtct tctcggcctt ttgttgaaaa acattgctgt ccgcatgact
     1861 ggcaaccgaa gcagaaagaa cactaaaaag caggactggc acacattttt tcacgggatt
     1921 attcctgact cattgaccat caagtcacat tgggagtaaa ccgacgtatg ataagagata
     1981 ctcttcggag atataactcc ctgagtatca agattaaaaa cgcaaggaga tgtttatgag
     2041 atctgccgct gtcaggctgc ttctgatacc tctgataaca gcaacaatag ctcttacagg
     2101 atgcacacca aagaccagcc tggaacgaca tacccggcat tatgtttatg cttcagatga
     2161 tggatttgat cctaacttcc acacccagaa agcagacacc atacgtatga tgcacccgtt
     2221 ctttcagcag ttccgggata tggggatgaa agacaaagca gccggagtat cagcagaaac
     2281 ggcacagcaa cgtgtaaaag aattccactc tgaagaattc cttcagtcac tgagaaccac
     2341 aaccaccttt gcgggcagaa aatacacgaa cagtgatatg ccatcaccgg aaaaaatgaa
     2401 gctgctggct gacacgattt ctgcggttta tctcgatggc tacgagggca ggcagtaagg
     2461 gagacattta ccataatccc ttattgtacg caccgctgaa acgcgttcag cgcgatcacg
     2521 gcagcagaca ggtaaaaatg gcaacaaacc acccgaaaaa ctgccgcgat cgcgcctgat
     2581 aaattttaac cgcatgaata cctatgcaac cagatggtac aggccacatt aacccctctt
     2641 aatccactga agctgccatt tttcatggtt tcaccatccc agcgaaggac catccagcgt
     2701 gcgttcctgt atttccggct gacgctcccg ttccagggat aacgcatgtt cgcgttcctg
     2761 tatcagccgt tcgtccctta tctcctgttc tcgctgtata actggctcaa gcgttctgtc
     2821 cgctcgctca agtgctgcac ctgctgactc aactgcatga cctgctcgtt cagcatcgcg
     2881 ttgtcccgtt gcgtaagcga aaacattttc tgcaattcca cgaaggcgct ctcccattcg
     2941 ctcagccgct gcatatagtc ctgttgcagt tgctctaatg cgttcagcaa atgtctttcc
     3001 agctccgtca ctctgtgtca ctccgtcagt tgtacccagt ccttcccctg atagggaatc
     3061 accgttgccg atttcccgta cggaaggacc agaaatttcc ttctcccgtc ctgcacaaac
     3121 tccacgcccc atgtcttcgc gttcagtttc tgcaatgtct cttcctgtat cctgatttct
     3181 tccaggttcg cctgtatccg cccgccaaga taccagagcg tcccgccact cgcggtaaac
     3241 aggagaaaga ctatccccag caacctcatg cccgtattcc ctgccagctt taacatgtcc
     3301 ttccggtgct gcatcatcgc ctctttcacc ccttcccggt gttttttcag cgattcctcc
     3361 gtcgaggccg taaactgggc tatagcgcct ttgattttcg tctcgtttga ggtcacagcc
     3421 tcgcttacag attcgccgag cttcctgaat tcgtcgttca gcattttctc tgtagattcg
     3481 gctctctctt tcagcttttt ctcgaactcc acgcccgtct gtaaaagatt gctcataaaa
     3541 tgctcctttc agcctgatat tcttcccgcc gttcgggtct gcaatgctga tactgcttcg
     3601 cgtcaccctg accacttcaa gccctgcctc tgtgagcgcc tgaatcacat cctgacggcc
     3661 tcttatctcc ccgacatgat aaagagcgtc tatcccgcgt gtgacgctct ctgcaagcgc
     3721 ctgtttcgtt tccggcaggt tatcagggag tgtcagtatc cggcggttct ccggggcgtt
     3781 ggggtcatgc agcccgtaat ggtgattcac cagcgtctgc caggcatcaa ttctcggcct
     3841 gtctgcccgg tcgtagtacg gctggaggcg ttttccggtc tgtagctcca tgttcggaat
     3901 gacaaaattc agctcaagcc gtcccttgtc ctggtgctcc acccacagga tgctgtactg
     3961 attcttttcg agaccgggca tcagtacgcg ctcaaagctc gccatcactt tttcacgtcc
     4021 tcccggcggc agctccttct ccgcgaacga cagaacgcct gacgtgtatt tcttcgcaaa
     4081 tggcgtggca tcgatgagtt cccggacttc ttccggatta ccctgaagca ccgttgcgcc
     4141 ttcgcggtta cgctccctcc ccagcaggta atcaaccgga ccactgccac caccttttcc
     4201 cctggcatga aatttaacta tcatcccgcg tcccctgttc cctgacggcc agacgcagcc
     4261 ggcgcagctc gtccccgatg gccatcagtg cggccaccac ctgaacccgg tcaccggaag
     4321 accactgccc gctgttcacc ttacgggctg tctgattcag gttatttccg atggcggcca
     4381 gctgacgcag taacggcggt gccagtgtcg gcagtttccc ggaacgggca accggctcac
     4441 ccagacagac ccgccgcatc cataccgcca gttgtttacc ctcacagcgt tccagtaacc
     4501 gggcatgttc atcatcagta acccgtatcg tgagcatcct ctcgcgtttc atcggtatca
     4561 ttacccccat gaacagaaat cccccttaca cggaggcatc agtgactaaa caggaaaaaa
     4621 ccgcccttaa catggcccgc tttatcagaa gccagacatt aacgctgctg gaaaagctca
     4681 acgaactgga cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgccgatg
     4741 agctttaccg cagctgcctc gcacgtttcg gggatgacgg tgaaaacctc tgacacatgc
     4801 agctcccgga gacggtcaca gcttgcctgt gagcggatgc cgggagcaga caagcccgtc
     4861 agggcgcgtc agcaggtttt agcgggtgtc ggggcgcagc cctgacccag tcacgtagcg
     4921 atagcggagt gtatactggc ttaactatgc ggcatcgatg cggattgtat gaaaagtgcg
     4981 ccatggacgg tgtgaaatgc cgcacagatg cgtaaggaga aaatgcaact tacgacactc
     5041 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtgcc
     5101 ggctcactaa aaggcggaaa tacggttatc cacagaatca ggggataagg ccggaaagaa
     5161 catgtgagta aaaaggcaaa ggacaaaaaa aggccgcgcc gctggcgttt ttccataggc
     5221 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     5281 caggactata aagataccag gcgtttcccc ccggaagctc cctcgtgcgc tctcctgttc
     5341 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     5401 ctcatagctc acgctgttgg tatctcggcc cggtgtaggt cgttcgctcc aagctgggct
     5461 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtctcg
     5521 agtccaaccc ggtaagacac gacaaagcgc cactggcagc agccattggt aactgaaaag
     5581 tggatttaga tacgcagaac tcttgaagtt gaagccttat agcggctaca ctgaaaggac
     5641 agcatttggt atctgtgctc cacttaagcc agctacccgg ttaagcagtc cccaactgac
     5701 ttaaccttcg actaaaccgc ctccccaggc ggttttttcg tttacagggc aagagactac
     5761 gacgatctgt aaaggatctc aagaagatcc tttacaggct accactacta ccacttccca
     5821 tactcactcc ggtaacagtg gaagatggtg aaacagcccc acctccagta tctctgacat
     5881 tgttgacttg gcatgctgca agcagcatta ttcctagtga aatggcacaa ataataattt
     5941 ttttcatata tacctttatt ataatcctat aggtcaatga tgattgatga acattcaata
     6001 gatatagaca atagaaaagc caataactta ttgtatttat ttatggtaat cggagttata
     6061 cctttactct gtatactagc cgtttactat acaaatccag acaatctgtt cttacacaca
     6121 atagctacca gtactgaaaa catcccatca ataacctccg cctacaaccc attaatgaca
     6181 aaggttatgg atatttattg taaaacagcg ccttcccttg ctttgatatt atttatcctg
     6241 acatttaaaa cgagaaaact aataaaaaaa acaaaccgaa atgctgtact tagaagttgc
     6301 ttattaagtc catttgtctg tgctgttttt ttgtatttac tctgcttccg aaaccttgag
     6361 ctaacgacag caggaaggcc agctaggcta atggccaaca atgatgcaac actattgcta
     6421 ttttatattg gcctgtactt aatcattttt tttataagct atttcacgct attcacacca
     6481 gttaccacgt ttaaattatt aaaagaaagg cagtaaccct gccttccttt tacccggtat
     6541 ttattaatga gcgggtttaa tgatttcgtt atttagtgca tctgcaaact tgtcatcaat
     6601 taatgcacct acaactgctg cgagtagaat acctacgata ccgacagcaa tagcaggagc
     6661 cccaagagat aaggcatatg cccctaatgt ggctgaaaaa acccccaggg ccacagcaga
     6721 agcaatgcca ctgagcaccc aggactccac ctccagcatc agaggtcccc agttcccggt
     6781 ttcatagccc tcgatgcttt tctcccggac cttctcaacc ttcatcacca catcggcaaa
     6841 tttgaaagcc ttgctgatat tacccagttt atttgccata tcctgagcat caatacttcg
     6901 ccaggcgttc gccagtgcat cactgtcggc
//
