LOCUS       CP057553                5674 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB23-C05 plasmid pRHB23-C05_9, complete
            sequence.
ACCESSION   CP057553
VERSION     CP057553.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148212
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 5674)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH12 T2-C05
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 147.407323264371x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 09:47:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,672
            Genes (coding)                    :: 4,532
            CDSs (with protein)               :: 4,532
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 56 of 140
            Pseudo Genes (incomplete)         :: 70 of 140
            Pseudo Genes (internal stop)      :: 35 of 140
            Pseudo Genes (multiple problems)  :: 20 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB23-C05"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB23-C05_9"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(5651..5674,1..903)
                     /locus_tag="HVX95_23920"
     CDS             join(5651..5674,1..903)
                     /locus_tag="HVX95_23920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QMJ58994.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKEHELVRMLQHDVAREHGFD
                     MRDRVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            906..1439
                     /locus_tag="HVX95_23925"
     CDS             906..1439
                     /locus_tag="HVX95_23925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMJ58995.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVTLED
                     KLAEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            1661..2785
                     /locus_tag="HVX95_23930"
     CDS             1661..2785
                     /locus_tag="HVX95_23930"
                     /inference="COORDINATES: protein motif:HMM:NF013211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMJ58996.1"
                     /translation="MSGGDGRGPGNTGLGHNGGKPSGNVNGSSGHGGPSSGGGYHYNPG
                     KGPASTVGPDGEVHINITGGMEKDPPGKHHGNGGSGNGHGAGNHNSNSGGNGKTTSIPG
                     WSPDSGKPGRYLPTNKDKLIKGLIQSIDDHSGTYTIHYTGGAVATVTVPNGDVNKMNVH
                     YNIGSGPSSGNKLTRDTIKSFLEFKAENEANAVNDAISKVNDFYKELASKYGDKLEKEA
                     KALADEAKGKKIRNANEAIKAFDRYKDVLNKKFSVSDREAIAKALESLNKDQMAKQLKI
                     FGKAFGVVGEAIQWGGFVSGLVKGFRTGDWNEAFISGEKIAVGKVASVMVTVAFSAMAV
                     NPIGILGFAVIMAVTSALITDERLKQLNSFINGI"
                     /name="hypothetical protein"
     gene            3586..4494
                     /locus_tag="HVX95_23935"
     CDS             3586..4494
                     /locus_tag="HVX95_23935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QMJ58997.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAIERALCEKLGADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASA
                     CRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPDYVRWLEAVNQRVEMRNASLPVPLSP
                     AECRAIGKSIAKYTHRKFSPEGFSAVQAARGRKGGIAKGEAYEDKRLMALCMLENGYSQ
                     KAIAAMLEVSTRTIRNWKSGK"
                     /name="replication initiation protein"
     gene            4719..5036
                     /locus_tag="HVX95_23940"
     CDS             4719..5036
                     /locus_tag="HVX95_23940"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMJ58998.1"
                     /translation="MPNLPKSVIAADICDSWVTRYSSHPMSKPRLVAFWKLMAKQFRVG
                     GRISRSEYRALKGHLAMAVVMVDSPSSFASPELRKAIVATALEAEEIIFIIEPDLKGFE
                     V"
                     /name="hypothetical protein"
     gene            complement(5082..5474)
                     /locus_tag="HVX95_23945"
     CDS             complement(5082..5474)
                     /locus_tag="HVX95_23945"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMJ58999.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAIQKRIEGKKQRRERNHRLFLAAGLLIEAGLMESKTG
                     NLKFKRELTLQALKRIRYELETSPEA"
                     /name="hypothetical protein"
     Typing          3587..4314
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.623"
                     /coverage="94.0"
                     /evalue="0.0"
     Typing          4341..4494
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.548"
                     /coverage="88.0"
                     /evalue="4.26e-58"
     Typing          2..900
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.667"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 aaatttggtg gcaaggggca ggctgcaaac cacgccgatt acatcgagcg caaagagaaa
       61 taccgcgacc gtcaggattt ggaatattcc gcacacggaa acatgccgga atgggcgcga
      121 gataatccct cacatttctg gcaggcggct gaccagtttg aacgtgcgaa cggttcgacc
      181 tatcgggaac ttgaaattgc attaccccgt gaactgacag cggaacagcg ccttgaactg
      241 gtgcaggatt ttgtacgtca ggaagctggc gaacggcacg cctggagttt tgccattcac
      301 aatccgaaag cgagcattga cggcggcgaa cagcctcatg cgcatatcat gatgagccag
      361 cgtgtgaatg atggcattga acgtacaccg gaacagtatt tccggcgcta taacgccagg
      421 tatcccgagc ggggaggtgc gaaaaaggat tccggaagcc tgactctcac tcagcaaaaa
      481 gagcagttgc gggaattgcg aaaacgctgg gaggtgaagc acaacgaaca tatgagaaag
      541 catggctttg aaaggggctt cattgactgc cggacgctga aagaacaggg cattgaacga
      601 cgtcccgaag tgcatctggg ttttgaagct gccggacggc ttgatgatgc ccagcgtcat
      661 gacatcatgc aaaaacggtc tgaaccggac tattcccgcc atcttgaaca ggcgcgaacg
      721 gtcgggcatg acatcaggga atggctggac actccggtta aggaacatga actggtcaga
      781 atgctgcaac atgatgttgc gcgggagcat ggttttgaca tgagagacag agtgagcaga
      841 cagaaccgaa ctgaacctga acgggttaaa tcaccgtcac gtggattttc tatgggattc
      901 tgaacatggc cagcacagaa gaaaaattac tcacgctcct gttatcggtg gagcatctgc
      961 aacataccgc aatggagcaa cagaaagctg ttgctgattc tctggctgtg gcaagggaaa
     1021 cacttcgcgg atatcgggat atttctgacg gtgtggcaaa acaggtcagg gatgaagtca
     1081 gaaacgaggt gcagaagctg gatgttgcag ggctgatagg tgagcgcatc agccaggatt
     1141 ttcagcggct ggaagagtca gtcgcaaaca tgaaggcaaa cgcggatgcc ttgaacaggg
     1201 atattgcgac aattcacagc aatatcgtgc aggaatacaa cacccttaaa gggtatagct
     1261 ggaaatggct gggtggggtg tcagtggctt ttctggttat gttctgtgtg cttggatggg
     1321 gaatgaaaac aatgctcgac aggaactaca acgccacagc ggcggtgtat cagaaagttg
     1381 tgacactgga agataaactg gcggagaaac ctgtcacggg caagagccgg aaaaactgac
     1441 gcgcttcgct tgtccaacgc ctcacgaccc gcgaaaggat ttcgcgttcc gttcaccgtt
     1501 ttcatgagtg attcctccga acagccaaaa aatatccctc ccctgacttg acagaaaaaa
     1561 taatggcgag tactttttga tctgtacata aaaccagtgg ttttatgtac agtattaact
     1621 atgtaatcaa ttgttttaac gcttaaaaga gggaattttt atgagtggtg gagatggtcg
     1681 tggccctggt aatacagggc ttggacataa cggtggtaaa cccagtggaa acgtgaatgg
     1741 ctcatctgga catggaggac catcatctgg tggtgggtat cattacaatc cgggaaaagg
     1801 tcctgcaagc actgtaggtc ctgacgggga ggtgcatatc aacattaccg gtggtatgga
     1861 aaaagatcct ccaggaaaac atcacggtaa tggtggctca ggtaatggtc acggtgctgg
     1921 aaatcataac agcaattcag gcggcaatgg aaaaacaacg tctattcctg gatggagccc
     1981 tgactctggg aaacctggca gatatttacc gacaaataaa gataagttaa ttaagggact
     2041 tattcagtca atagatgacc attctggaac gtacactatc cattatactg gtggtgcagt
     2101 agctacagta actgtaccaa acggtgatgt gaataaaatg aatgttcact ataatatcgg
     2161 tagtgggcct tcatcaggta ataaactgac tcgtgatact ataaaaagct ttttagaatt
     2221 caaggcagaa aatgaagcta atgctgtaaa tgatgcaata agcaaagtta atgactttta
     2281 taaagagtta gcatcaaaat acggcgataa gttggagaaa gaagccaagg cactagccga
     2341 tgaagctaaa gggaaaaaaa ttcgcaatgc taatgaggcg attaaagcct ttgataggta
     2401 caaggatgtg cttaataaga aattcagcgt atccgaccga gaagcgattg caaaagcact
     2461 ggagtctctt aataaagatc aaatggctaa acagttaaaa atttttggta aggcctttgg
     2521 tgtcgtaggt gaggctattc agtggggggg atttgtcagt ggcttagtta aaggattcag
     2581 aactggtgac tggaatgagg cttttataag cggggaaaaa atcgcagtcg gcaaggtagc
     2641 atctgttatg gttacagtgg catttagtgc tatggcagta aatcctattg gtattttagg
     2701 gtttgcagta attatggcag tgactagtgc cttaattaca gatgaaaggc tgaaacaact
     2761 aaacagcttt attaacggca tttaataaac aaaaggtcgt accgtttgat atggtatgac
     2821 ctttattttg tttttctatc gctttttgtt ggttataaag taatagactg taaaaatcaa
     2881 tccaaaagga ggtagtgata atataatgca tattataaaa ccccaattaa cgaagttaaa
     2941 agttgttttt gttagtaagg gagtttgctt atttattagt aatagacaaa aagggtatgt
     3001 ggacgatcca aaaagagata taagtataaa tataaataat tctatacttg ttgggcttag
     3061 attcaaactt gcgatgataa aagataagat gtaaaaggat aaggcaagga taaaattcct
     3121 taaatagaat tccatatcta tttttttcat ttataaaatc ctcgttagtt aaatgtttat
     3181 aatattgcct tactttatcc ataaatctag aagtagttat cacaaaagtt acttttttga
     3241 ctcgttcgcc cttctgaacg ttcgaaactg tttgcgaatc gctttcgtgc atagttatgc
     3301 aacgccctga aagtgattct gacgcatttt tctggtttgg cctggtgttt ccttgtcttt
     3361 ttgcgttttt tgcgccagaa agcgactgag ggcgttttaa ggggtacgta caacgggagt
     3421 tatggtaaat ggctcggatt tgcgggaagg ctcgacaggt tttgtcgttg ggggtagtgt
     3481 aagcgactga aaaacaaacg cccctgaaat tcgtgtctct ccgccaagac tttcacgaaa
     3541 atacaggggc gatgtcggtc gataacctgg aaggaatcgt aacacatgag cgccgcgctt
     3601 caatacttcg aagaaaattt accccaccgt ccctatcaca cggatgatct cgctttcggc
     3661 cttcgtatct ccggcaaagg gcgcgcgctt cttgcacggt atatccagca gaaccagcct
     3721 catgcacagt tctggctggt ttttgacgtt gaccgcgagg gggctgctat tgactggagc
     3781 gaccggaacg cccccgcgcc gaacatcacc gtaaaaaatc ctgtgaacgg tcacgcccac
     3841 ctgctctacg cactcaatat cgccgtgaga accgcgccag atgcctcggt taaggcgctg
     3901 aaatacgctg ccgcgataga gcgtgcgttg tgtgagaaac ttggcgcaga tgtgaattac
     3961 agcggactga tttgtaaaaa tccgttccac ctggaatggc aggtgatgga gtggcgcgag
     4021 gaagcctata ccctcgatga actggctgat tatctcgatt tgagcgcctc agcgtgccgt
     4081 agcatcgata aacattacgg gatggggcga aactgccacc tgttcgaaat gacgcgcaaa
     4141 tgggcttaca gggcgattcg gcagggctgg cccgattacg ttcgctggct ggaagcggtg
     4201 aaccagcgtg tcgaaatgcg caacgcatcg cttcccgtac cgctttctcc ggctgaatgt
     4261 cgggctattg gcaagagtat tgcgaaatac acgcacagga aattctcgcc ggaaggtttt
     4321 tccgctgtac aggctgcacg aggccggaaa ggtggcatcg caaaaggcga agcctacgag
     4381 gacaagcgct taatggcatt gtgtatgctg gagaatggat attctcagaa agctattgct
     4441 gcgatgttgg aggtttctac tcgaactatt cgaaactgga aaagcggaaa atagccttat
     4501 cagataacag cgcctttttg gcgttttttt gagccgtagg ttttccggcg gttttctctg
     4561 ctcagcccta tgatggtgcg gctttctgca tgattgaggt ggtagtgctc gccgcagcct
     4621 catgaccgag cgttcgcgag cgaatgagcg aggaagcacc agggaaccgg aacctagtgg
     4681 tggctctttc gcgctgtcgt ttattttcaa gaggttatat gcctaactta cccaaaagtg
     4741 ttattgctgc cgacatctgc gattcttggg tgactcggta ttcttctcat cccatgagca
     4801 agccgcgtct tgtcgctttc tggaaattga tggctaaaca gtttcgtgta ggtggccgga
     4861 ttagccgtag tgagtacagg gcgttgaaag gccatcttgc tatggctgtc gtcatggttg
     4921 attcccctag ttcatttgca tctccggaac tacgaaaagc tattgttgcc acagccttgg
     4981 aagcggagga gatcattttt atcatcgaac ctgacttaaa gggcttcgag gtttaaattg
     5041 gctgtttact aaacggaaaa atggtcctgg aatagggact cttacgcctc tgggcttgtt
     5101 tccagctcat acctgatccg tttaagtgcc tgtaacgtaa gctctctttt gaacttcagg
     5161 ttgcctgttt ttgattccat tagtcccgct tcgatgagta atcctgccgc cagaaagagc
     5221 cggtgattgc gttcccgtcg ttgttttttt ccctcgattc gtttctggat cgcctgtttt
     5281 cgttcgctct cctgtgcctg taaatcgttt ttttccgctg ttgccagtac gtggagaagt
     5341 ttgcgctcct gttctgtgag tccggcttcg ttgtcgagca ggtcaataat ctgtctctgt
     5401 cgcgcggttt gttctccgag cttcctgatg aatccgatcc gtttcttcag ccattcgatg
     5461 ttgctgatgc gcatgttcgt ggtgctccct ggtgtttttt tgcggtgtgt tgtggtcgtg
     5521 cagtatatcg cttgcggtcg gtgggtgcag ggggtatctt ccgtgctaat tcgtaaaatt
     5581 agaaggaagc gcacttatat gtttctctcc ttcgtcgaga aacagtgcgc tcctgcggag
     5641 ggctgaaacc gtggcaacct atcacctcag cgtt
//
