LOCUS       CP057550                6930 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB23-C05 plasmid pRHB23-C05_6, complete
            sequence.
ACCESSION   CP057550
VERSION     CP057550.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148212
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH12 T2-C05
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 147.407323264371x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 09:47:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,672
            Genes (coding)                    :: 4,532
            CDSs (with protein)               :: 4,532
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 56 of 140
            Pseudo Genes (incomplete)         :: 70 of 140
            Pseudo Genes (internal stop)      :: 35 of 140
            Pseudo Genes (multiple problems)  :: 20 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB23-C05"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB23-C05_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(5545..6930,1..114))
                     /gene="csa"
                     /locus_tag="HVX95_23820"
     CDS             complement(join(5545..6930,1..114))
                     /gene="csa"
                     /locus_tag="HVX95_23820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /protein_id="QMJ58975.1"
                     /translation="MAKELSGYGPTAGESVGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGGEHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPGAGGNSDVQG
                     GYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEK
                     AKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDNLPPNVRKFDVDVEQF
                     HYLVVLDDYGNVLSITRTGVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEEPQRQ
                     NQAKKAAYDVFYSFSMNRDRIQSDVLNKAAEVISDIGNKVGDYLGNAYKSLAREIADDV
                     KNFQGKTIRSYDDAMASLNKVLSNPGFKFNRADSDALANAWRSIDAQDMANKLGNISKA
                     FKFADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASAVALGVFSATLGAYAL
                     SLGAPAIAVGIVGILLAAVVGALIDDKFADALNNEIIKPAH"
                     /name="csa"
     gene            355..645
                     /locus_tag="HVX95_23825"
     CDS             355..645
                     /locus_tag="HVX95_23825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMJ58976.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            complement(637..915)
                     /locus_tag="HVX95_23830"
     CDS             complement(637..915)
                     /locus_tag="HVX95_23830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMJ58977.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLASLERQKKSLR"
                     /name="hypothetical protein"
     gene            1037..1459
                     /gene="exc2"
                     /locus_tag="HVX95_23835"
     CDS             1037..1459
                     /gene="exc2"
                     /locus_tag="HVX95_23835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMJ58978.1"
                     /translation="MRSAAVRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFHTQKADTIRMMHPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEEFLQSLRTTTT
                     FAGRKYTNSDMPSPEKMKLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(1779..2012)
                     /gene="mbeD"
                     /locus_tag="HVX95_23840"
     CDS             complement(1779..2012)
                     /gene="mbeD"
                     /locus_tag="HVX95_23840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMJ58979.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="mbeD"
     gene            complement(2019..2537)
                     /locus_tag="HVX95_23845"
     CDS             complement(2019..2537)
                     /locus_tag="HVX95_23845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QMJ58980.1"
                     /translation="MSNLLQTGVEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIKGAIAQFTASTEESLKKHREGVKEAMMQHRKDMLKLAGNTGMRLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVLPYGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            complement(2515..3225)
                     /locus_tag="HVX95_23850"
                     /pseudo=""
     CDS             complement(2515..3225)
                     /locus_tag="HVX95_23850"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3215..3538)
                     /locus_tag="HVX95_23855"
     CDS             complement(3215..3538)
                     /locus_tag="HVX95_23855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMJ58984.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3603..3794
                     /locus_tag="HVX95_23860"
     CDS             3603..3794
                     /locus_tag="HVX95_23860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMJ58981.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4793..4948)
                     /gene="csl"
                     /locus_tag="HVX95_23865"
     CDS             complement(4793..4948)
                     /gene="csl"
                     /locus_tag="HVX95_23865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="QMJ58982.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GSSGSL"
                     /name="csl"
     gene            4978..5517
                     /gene="csi"
                     /locus_tag="HVX95_23870"
     CDS             4978..5517
                     /gene="csi"
                     /locus_tag="HVX95_23870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 immunity protein"
                     /protein_id="QMJ58983.1"
                     /translation="MMIDEHSIDIDNRKANNLLYLFMVIGVIPLLCILAVYYTNPDNLF
                     LHTIATSTENIPSITSAYNPLMTKVMDIYCKTAPSLALILFILTFKTRKLIKKTNRNAV
                     LRSCLLSPFVCAVFLYLLCFRNLELTTAGRPARLMANNDATLLLFYIGLYLIIFFISYF
                     TLFTPVTTFKLLKERQ"
                     /name="csi"
     Typing          complement(3880..3967)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          3990..4536
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.989"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1676..3225)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.412"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccagtgaacc ccactattac tattattatt tccaccttgc tgattcagat tcgcccctgt
       61 tccccccaca gactcaccag cagttgggcc atatccactt agttctttag ccataaattc
      121 ctctttgata attaaaacaa taaattaaaa gcaatatact gtacatataa ccactggttt
      181 tgtattcagc aaaaacctac tactcagcat tatccatgtc aagagcatgg attttcattt
      241 ttgcaataag gatcacacta tggggaggca gacattgaga acgtcgaaac agaacaccgg
      301 agcaaatcag gatgagatat aaaactgttg gatcatgaaa aaacggagaa cgatgtgagc
      361 aaatctcccc gccataaact gaacaaaaca gacaagcgac ttctcgacac cctggttgct
      421 gccggatatg agcatgacaa agcccgtgac ctcatccaga aacaggttta cacgctgaca
      481 ctggctgatc agcgtcatgt ggtcagtgaa atcagtaacg gtgtgaatcc cacccaggct
      541 tactcggcgg tataccaggc aagacgcatt cgcctcgccc gtaaatatct gaacggtaaa
      601 aaggttatgg aagaaaccgg ggaaaatacg cccccatcag cgtaaggatt tcttttgccg
      661 ctccagagac gccagttttt tacgcaaatc ctctcttttt tgggcatctc tggtgccagc
      721 cagctctgct ctcaactcat cgatctgaag ttgtatcttc agtctgttac tgaacatttt
      781 ctgtctggca ttaacatccg caacaatgcc gttttttgtc ttctcggcct tttgttgaaa
      841 aacattgctg tccgcatgac tggcaaccga agcagaaaga acactaaaaa gcaggactgg
      901 cacacatttt ttcacgggat tattcctgac tcattgacca tcaaatcaca ttgggagtaa
      961 accgacgtat gataagagat actcttcgga gatataactc cctgagtatc aagattaaaa
     1021 acgcaaggag atgtttatga gatctgccgc tgtcaggctg cttctgatac ctctgataac
     1081 agcaacaata gctcttacag gatgcacacc aaagaccagc ctggaacgac atacccggca
     1141 ttatgtttat gcttcagatg atggatttga tcctaacttc cacacccaga aagcagacac
     1201 catacgtatg atgcacccgt tctttcagca gttccgggat atggggatga aagacaaagc
     1261 agccggagta tcagcagaaa cggcacagca acgtgtaaaa gaattccact ctgaagaatt
     1321 ccttcagtca ctgagaacca caaccacctt tgcgggcaga aaatacacga acagtgatat
     1381 gccatcaccg gaaaaaatga agctgctggc tgacacgatt tctgcggttt atctcgatgg
     1441 ctacgagggc aggcagtaag ggagacattt accataatcc cttattgtac gcaccgctga
     1501 aacgcgttca gcgcgatcac ggcagcagac aggtaaaaat ggcaacaaac cacccgaaaa
     1561 actgccgcga tcgcgcctga taaattttaa ccgcatgaat acctatgcaa ccagatggta
     1621 caggccacat taacccctct taatccactg aagctgccat ttttcatggt ttcaccatcc
     1681 cagcgaagga ccatccagcg tgcgttcctg tatttccggc tgacgctccc gttccaggga
     1741 taacgcatgt tcgcgttcct gtatcagccg ttcgtccctt atctcctgtt ctcgctgtat
     1801 aactggctca agcgttctgt ccgctcgctc aagtgctgca cctgctgact caactgcatg
     1861 acctgctcgt tcagcatcgc gttgtcccgt tgcgtaagcg aaaacatttt ctgcaattcc
     1921 acgaaggcgc tctcccattc gctcagccgc tgcatatagt cctgttgcag ttgctctaat
     1981 gcgttcagca aatgtctttc cagctccgtc actctgtgtc actccgtcag ttgtacccag
     2041 tccttcccct gatagggaat caccgttgcc gatttcccgt acggaaggac cagaaatttc
     2101 cttctcccgt cctgcacaaa ctccacgccc catgtcttcg cgttcagttt ctgcaatgtc
     2161 tcttcctgta tcctgatttc ttccaggttc gcctgtatcc gcccgccaag ataccagagc
     2221 gtcccgccac tcgcggtaaa caggagaaag actatcccca gcaacctcat gcccgtattc
     2281 cctgccagct ttaacatgtc cttccggtgc tgcatcatcg cctctttcac cccttcccgg
     2341 tgttttttca gcgattcctc cgtcgaggcc gtaaactggg ctatagcgcc tttgattttc
     2401 gtctcgtttg aggtcacagc ctcgcttaca gattcgccga gcttcctgaa ttcgtcgttc
     2461 agcattttct ctgtagattc ggctctctct ttcagctttt tctcgaactc cacgcccgtc
     2521 tgtaaaagat tgctcataaa atgctccttt cagcctgata ttcttcccgc cgttcgggtc
     2581 tgcaatgctg atactgcttc gcgtcaccct gaccacttca agccctgcct ctgtgagcgc
     2641 ctgaatcaca tcctgacggc ctcttatctc cccgacatga taaagagcgt ctatcccgcg
     2701 tgtgacgctc tctgcaagcg cctgtttcgt ttccggcagg ttatcaggga gtgtcagtat
     2761 ccggcggttc tccggggcgt tggggtcatg cagcccgtaa tggtgattca ccagcgtctg
     2821 ccaggcatca attctcggcc tgtctgcccg gtcgtagtac ggctggaggc gttttccggt
     2881 ctgtagctcc atgttcggaa tgacaaaatt cagctcaagc cgtcccttgt cctggtgctc
     2941 cacccacagg atgctgtact gattcttttc gagaccgggc atcagtacgc gctcaaagct
     3001 cgccatcact ttttcacgtc ctcccggcgg cagccccttc tccgcgaacg acagaacgcc
     3061 tgacgtgtat ttcttcgcaa atggcgtggc atcgatgagt tcccggactt cttccggatt
     3121 accctgaagc accgttgcgc cttcgcggtt acgctccctc cccagcaggt aatcaaccgg
     3181 accactgcca ccaccttttc ccctggcatg aaatttaact atcatcccgc gtcccctgtt
     3241 ccctgacggc cagacgcagc cggcgcagct cgtccccgat ggccatcagt gcggccacca
     3301 cctgaacccg gtcaccggaa gaccactgcc cgctgttcac cttacgggct gtctgattca
     3361 ggttatttcc gatggcggcc agctgacgca gtaacggcgg tgccagtgtc ggcagtttcc
     3421 cggaacgggc aaccggctca cccagacaga cccgccgcat ccataccgcc agttgtttac
     3481 cctcacagcg ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc
     3541 tctcgcgttt catcggtatc attaccccca tgaacagaaa tcccccttac acggaggcat
     3601 cagtgactaa acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat
     3661 taacgctgct ggaaaagctc aacgaactgg acgcggatga acaggcagac atctgtgaat
     3721 cgcttcacga ccacgccgat gagctttacc gcagctgcct cgcacgtttc ggggatgacg
     3781 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgcctg tgagcggatg
     3841 ccgggagcag acaagcccgt cagggcgcgt cagcaggttt tagcgggtgt cggggcgcag
     3901 ccctgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcgat
     3961 gcggattgta tgaaaagtgc gccatggacg gtgtgaaatg ccgcacagat gcgtaaggag
     4021 aaaatgcaac ttacgacact cttccgcttc ctcgctcacg gactcgctgc gctcggtcgt
     4081 tcggctgcgg cgagcggtgc cggctcacta aaaggcggaa atacggttat ccacagaatc
     4141 aggggataag gccggaaaga acatgtgagt aaaaaggcaa aggacaaaaa aaggccgcgc
     4201 cgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     4261 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cccggaagct
     4321 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     4381 cttcgggaag cgtggcgctt tctcatagct cacgctgttg gtatctcggc ccggtgtagg
     4441 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     4501 tatccggtaa ctatcgtctc gagtccaacc cggtaagaca cgacaaagcg ccactggcag
     4561 cagccattgg taactgaaaa gtggatttag atacgcagaa ctcttgaagt tgaagcctta
     4621 tagcggctac actgaaagga cagcatttgg tatctgtgct ccacttaagc cagctacccg
     4681 gttaagcagt ccccaactga cttaaccttc gactaaaccg cctccccagg cggttttttc
     4741 gtttacaggg caagagacta cgacgatctg taaaggatct caagaagatc ctttacaggc
     4801 taccactact accacttccc atactcactc cggtaacagt ggaagatggt gaaacagccc
     4861 cacctccagt atctctgaca ttgttgactt ggcatgctgc aagcagcatt attcctagtg
     4921 aaatggcaca aataataatt tttttcatat atacctttat tataatccta taggtcaatg
     4981 atgattgatg aacattcaat agatatagac aatagaaaag ccaataactt attgtattta
     5041 tttatggtaa tcggagttat acctttactc tgtatactag ccgtttacta tacaaatcca
     5101 gacaatctgt tcttacacac aatagctacc agtactgaaa acatcccatc aataacctcc
     5161 gcctacaacc cattaatgac aaaggttatg gatatttatt gtaaaacagc gccttccctt
     5221 gctttgatat tatttatcct gacatttaaa acgagaaaac taataaaaaa aacaaaccga
     5281 aatgctgtac ttagaagttg cttattaagt ccatttgtct gtgctgtttt tttgtattta
     5341 ctctgcttcc gaaaccttga gctaacgaca gcaggaaggc cagctaggct aatggccaac
     5401 aatgatgcaa cactattgct attttatatt ggcctgtact taatcatttt ttttataagc
     5461 tatttcacgc tattcacacc agttaccacg tttaaattat taaaagaaag gcagtaaccc
     5521 tgccttcctt ttacccggta tttattaatg agcgggttta atgatttcgt tatttagtgc
     5581 atctgcaaac ttgtcatcaa ttaatgcacc tacaactgct gcgagtagaa tacctacgat
     5641 accgacagca atagcaggag ccccaagaga taaggcatat gcccctaatg tggctgaaaa
     5701 aacccccagg gccacagcag aagcaatgcc actgagcacc caggactcca cctccagcat
     5761 cagaggtccc cagttcccgg tttcatagcc ctcgatgctt ttctcccgga ccttctcaac
     5821 cttcatcacc acatcggcaa atttgaaagc cttgctgata ttacccagtt tatttgccat
     5881 atcctgagca tcaatacttc gccaggcgtt cgccagtgca tcactgtcgg ccctgttaaa
     5941 tttaaaccca ggattagaca aaactttatt taatgacgcc attgcatcat cgtagctacg
     6001 gatggttttc ccctggaaat ttttgacatc atcagcaatt tctcttgcta acgacttata
     6061 agcatttcca agataatcac cgaccttatt acctatatca gagataactt cagcagcttt
     6121 attaagtaca tcactctgaa tccggtcacg gttcatactg aaagaataaa acacatcata
     6181 agcagctttt ttagcttgat tctgtcgttg aggctcttca ttattaaatc ctaatgcctc
     6241 atatatctct tccggggtct tatggtcgac tttatccata ataccagcct tagctttttc
     6301 actccctaca taaggacgaa cacctgttct agttatagat aaaacattac cataatcatc
     6361 aagcaccact aaatagtgga actgttcaac atcaacatca aattttctaa cattaggagg
     6421 caaattatcc cacgcatctt cagcctgttg ttttgctttt tcctgccgct gagattcatc
     6481 tttattaaaa cctaacgcct catatatctc ctcaggtgtt ttatgattaa ttttttccat
     6541 aatacccgcc ttagcttttt cactccctac gtaaggacgg acacctgttc gaagaatacc
     6601 gatagctcta ccattatcat taagcgttac acgatattta aaaccatcga tactcacatc
     6661 aatctctcgc ggtaagttgt ttttatccca cttacgagca acttcatcat taggattttc
     6721 aggaatatag cccccctgaa catcactatt cccgccagcc ccaggaactc catatacagg
     6781 tgtttttacc agaccatttg tgatggtaat gcgcacctgc ccattatcat caacataagg
     6841 cggcacatcc ggattattgg ttttactcca tccccacccc gtctgacttc catgttcacc
     6901 acctccatta ccagagcctg aaccaccacc
//
