LOCUS       CP057542                7198 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB23-C06 plasmid pRHB23-C06_4, complete
            sequence.
ACCESSION   CP057542
VERSION     CP057542.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148213
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 7198)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH12 T2-C06
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 126.333533647315x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 09:57:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,801
            CDSs (total)                      :: 4,685
            Genes (coding)                    :: 4,615
            CDSs (with protein)               :: 4,615
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 27 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 10 of 70
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB23-C06"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB23-C06_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(6966..7198,1..286)
                     /locus_tag="HVX96_23915"
     CDS             join(6966..7198,1..286)
                     /locus_tag="HVX96_23915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QMJ53544.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKVLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMRQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            293..526
                     /gene="mbeD"
                     /locus_tag="HVX96_23920"
     CDS             293..526
                     /gene="mbeD"
                     /locus_tag="HVX96_23920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMJ53545.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSRTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="mbeD"
     gene            complement(844..1263)
                     /gene="exc2"
                     /locus_tag="HVX96_23925"
     CDS             complement(844..1263)
                     /gene="exc2"
                     /locus_tag="HVX96_23925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMJ53546.1"
                     /translation="MRLTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSKLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(1344..1781)
                     /locus_tag="HVX96_23930"
     CDS             complement(1344..1781)
                     /locus_tag="HVX96_23930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QMJ53547.1"
                     /translation="MGHCNFMAWHTLQDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKQNETQERHTVGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRHQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            2007..3896
                     /gene="cra"
                     /locus_tag="HVX96_23935"
     CDS             2007..3896
                     /gene="cra"
                     /locus_tag="HVX96_23935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin R"
                     /protein_id="QMJ53548.1"
                     /translation="MVCFKSQRGMNMPGFNYGGYGDGTGWSSESGGPAPGGGMHGNSGG
                     QRGDNANSSNSVSQQISAIQNDQKLKQKVVNMLIAARKMNPEAKMILGSIAPSGVMQVT
                     IEGVTSTQARQLGLGGLVMGYNASGVIGAVGEIDTGHRLNASGASTPGSETSVESFVNG
                     QKPAGEWHAVAKDSWTGAGPVNVGLVNNAIKSVRIIKKGYVTGVLTPEEVMNKAEYKAM
                     RQAFDSLPLAKQGEAVRQIVAAWSLAYQDFPVNLKKEMGRVTERIVDAINLALILNQTE
                     SRLSESQKNVDVANQIISDTVKAINDVNKKIAEKRNQQVSLTDLMNKKQKEVEDLKKIF
                     KNHSYHRIRDAQREYDDARNKYALLASDINALQAQVSGLTARKQQAEQNKAAAEKAKAD
                     AAAKAAAEKAAAEAKAKAEAEKARKEAEEKANDEKAVLTKASEIIISVGDKAGEYLGDK
                     YKVLSREIADNIKNFQGKTIRSYDEAMASVNKLMANPDLKINAADRDAIVNAWKAFDAE
                     DMGNKFAALGKTFKAADYVMKANNVREKSIEGYQTGNWGPLMLEIESWVLSGIASAVAL
                     SFFSVAVGSLFITAGVSVTAVGLMGIIFAGFIGALIDDDFVDKLNNEIIRPAY"
                     /name="cra"
     gene            complement(3924..4448)
                     /gene="cri"
                     /locus_tag="HVX96_23940"
     CDS             complement(3924..4448)
                     /gene="cri"
                     /locus_tag="HVX96_23940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_089619593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin R immunity protein"
                     /protein_id="QMJ53549.1"
                     /translation="MQKEYTYNKDLNRMLFLITFAGIIPLIIIFITYTSNPNFYLIRII
                     FDNTQNIPSVISSYNPVMTKVMDIYGKSAPLLAFIAFIMLFKHTRTPKITDRKKIITAC
                     ILSPFLCIFYAYFFLWHNFELTTSGRPVRLMSENDLTLLIFYICLYYCSFFITYALCLQ
                     PVIAYKLWKER"
                     /name="cri"
     gene            4568..4708
                     /gene="crl"
                     /locus_tag="HVX96_23945"
     CDS             4568..4708
                     /gene="crl"
                     /locus_tag="HVX96_23945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin R system lysis protein"
                     /protein_id="QMJ53550.1"
                     /translation="MKMILSGGMLVLMALLSGCQANYIRDVQGGTVAPSSSSELTGIAV
                     Q"
                     /name="crl"
     gene            complement(5709..5900)
                     /locus_tag="HVX96_23950"
     CDS             complement(5709..5900)
                     /locus_tag="HVX96_23950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMJ53551.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5965..6288
                     /locus_tag="HVX96_23955"
     CDS             5965..6288
                     /locus_tag="HVX96_23955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMJ53552.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPLARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          5537..5624
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          complement(4968..5514)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.082"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2..630
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.088"
                     /coverage="100.0"
                     /evalue="1.0499999999999999e-94"
     Typing          6279..7198
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.44"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaaggacgtg ttaaagctgg cagggaatac gggcatgatg ttactgggga tagtctttct
       61 cctgtttacc gcgagtggcg ggacgctctg gtatcttggc gggaggatac aggcgaacct
      121 ggaagatatc aggaagcagg aagagacatt gcagaaactg aacgcgaaga catggggcgt
      181 ggagttcgtg caggacggga acaggaaatt tctggtcctt ccgtacggga aatcagcaga
      241 ggtgattccc tttcagggga aagagtgggt acatctgaag gagtgacaca gaatgacaga
      301 gctggaaaca catttgctga acgccttaga gcagctgcaa caggactata tgcagcggct
      361 gagcgaatgg gagagcgcct tcgtggaatt gcagaagatg ttttcgcgta cgcaacggga
      421 caacgcgatg ctgaacgagc gggtcatgca gttgagtcag caggtgcagc acttgagcga
      481 gcagacagaa cgcttgagcc agttatacag cgagaactgg agataagaga ggaacggctg
      541 atacaggagc gcgaacatgc gttatcccta gagcgtgagc gccagctgga aatacaggaa
      601 cgcacactgg atggtccttc gctgggatgg tgaaactatg aaaaatggca ggttaagtgg
      661 attaagacgg gctaatgtgg tctgtaccat ctggttgcat aggtattcat gcggttaaaa
      721 tttatcaggc gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc
      781 tgccgtgatc gcgctgaacg cgttttggcg gtgcgtacaa ttaagggatt atggtaaatc
      841 cacttactgt cttccctcgt agccatcgag ataaaccgca gaaatcgtgt cagccagcag
      901 cttggatttt ttcggggtaa ggtctttgct gcggtaatca gtacccgcaa attgagttgt
      961 gccccggagt gagtttaaaa attcttggct gtgaaactgc tgaatgcgtt gttgcacatc
     1021 actccgtgat tttcctgtcg ctttatcttt agctcccata tcccagaact gccgaaaaaa
     1081 aggcaccatc attcgtgttg tgtcggcttt ttgggtggaa aagttgggat caaaaccgtc
     1141 atcagaagca tatacgtaat gccgggtatg ttgttccacg ctgccctttg gggtacaggc
     1201 tgtgagagct actgttgctg ttatcatggc gataagacaa agtttgtttc cggtaagtct
     1261 catgagtgtc tccctacctt aaagtattta ttctcggtga gttattatcc gaagactatt
     1321 ttatcatcag tctctgacag cgttcagcga ccgaacaacc tggaccagaa tccccgtttc
     1381 ttttccagtt ccagtgcctg cctgagctgg gctatctcat tttgtagctg ttcccgttct
     1441 gcttcctggt gtctgcgatc catatcctgt gcctgtttat cctcaagcat cagcgtcagg
     1501 cattgtttca gctcattcag ttcccggaga atgcgttctg tctgctgatc atgtggattt
     1561 tctgcctgtc ccacagtgtg cctttcttgt gtctcatttt gctttaattc gccgtatgcc
     1621 cggatcagtt cactggtttc aaactccctt cgtccgtcct ggctggtgcg gtaactgaca
     1681 agacctgagc gcatatcacg gtaaaactgt gaacgcgatc ttccagtcat tttcagtgcg
     1741 tcctggagtg tgtgccatgc cataaagtta cagtgtccca taggtgtctc atctcatagt
     1801 ttcagtaaaa cataatgagg tctgagaacg gtaatgtttg cgccggtttt tgtggtttcc
     1861 ggcgggaata gcgcgtggtg tgagagactg tttttttgat cgttttcaca aaaatggaag
     1921 tccacactct tgacagggaa aatgcagcgg cgtagctttt atactgtaca taaagccagt
     1981 ggttatgtgt acagtatttg tttttaatgg tttgttttaa aagtcaaaga ggaatgaata
     2041 tgccggggtt taattacggt gggtatggtg acggtactgg ctggagttct gaaagtggtg
     2101 gtccagctcc aggcggtggt atgcatggaa atagtggagg ccagcgcgga gataacgcaa
     2161 acagtagcaa ttctgtgagt cagcagattt ctgccattca gaatgatcag aaacttaaac
     2221 agaaagtggt gaatatgctg attgctgcga gaaaaatgaa tcctgaagca aaaatgattc
     2281 tgggaagtat tgctccttct ggtgttatgc aggtcaccat tgagggggta acctccactc
     2341 aggccaggca gcttggtttg ggagggctgg tcatgggata taacgcttct ggtgttattg
     2401 gggctgtggg cgaaatagac accggtcacc gccttaatgc atcaggagca agtactcccg
     2461 gaagcgaaac ttctgtggag agttttgtga acggacagaa accagccgga gaatggcatg
     2521 ctgtggcgaa ggacagctgg acaggggctg gtccagtgaa tgtagggctg gttaataacg
     2581 ccatcaaaag tgtccggatt atcaaaaaag gctatgtgac gggcgttctc acgccagaag
     2641 aggtgatgaa taaagcagag tacaaagcaa tgcggcaggc tttcgattcc cttccactgg
     2701 caaaacaggg agaggctgtc agacagattg ttgctgcttg gagtctggca tatcaggatt
     2761 ttccggtaaa tctgaaaaaa gagatggggc gtgtcactga acggattgtt gatgctatta
     2821 acctggcgct cattcttaat cagacagagt cccgtttgtc ggaatcacaa aagaatgttg
     2881 atgtagcgaa ccagattatc agtgatacag taaaagctat taatgatgta aataagaaaa
     2941 tagcagaaaa acgaaatcag caggtatcac tgactgattt gatgaataaa aaacaaaaag
     3001 aggtcgaaga tcttaaaaag atattcaaaa atcactctta tcaccgtatt cgtgatgccc
     3061 agagggaata tgatgatgcc cgaaataaat atgctttact ggccagtgat attaatgcgc
     3121 tgcaggcaca ggtttccgga ctgacggcaa ggaaacagca ggcagaacag aacaaggcag
     3181 cagcagaaaa agcaaaggca gatgcagcgg cgaaagctgc agcagaaaaa gcggctgctg
     3241 aagcgaaagc aaaggctgaa gctgaaaaag ccagaaagga agcagaggaa aaagcaaatg
     3301 acgagaaggc tgttctgaca aaagccagtg aaattatcat cagtgttggt gataaggctg
     3361 gggagtatct gggtgataaa tataaagttc tttctcgtga gatagcagat aatatcaaaa
     3421 actttcaggg caaaacgatt cgtagttatg atgaggcgat ggcttctgtc aataaactga
     3481 tggctaatcc tgatcttaaa attaatgccg ccgacaggga tgcaattgtg aatgcctgga
     3541 aagcatttga tgcagaggat atggggaata agtttgccgc gctgggtaag acgtttaaag
     3601 ccgcagatta tgtgatgaag gcaaacaacg tccgggagaa gagtatcgag gggtatcaga
     3661 ccggaaactg ggggccgctg atgctggaga ttgagtcctg ggtattgagt ggtattgctt
     3721 ctgctgtggc gctatcattt ttctcagtcg ctgttggttc attatttatt acagcgggcg
     3781 tgtctgtaac ggctgtggga cttatgggca ttatttttgc tggctttatc ggtgcgctga
     3841 ttgatgatga ttttgttgat aagttaaata atgaaattat tcgccctgcg tattaattga
     3901 gagtcgaaag gtagtcagga ggactacctt tctttccaga gtttatatgc tattactggt
     3961 tggaggcaga gggcgtaagt tatgaaaaaa gagcaataat acaggcagat ataaaagata
     4021 agcaaagtaa gatcgttctc agacatcaat cgaacaggtc ttcccgatgt tgttaactcg
     4081 aagttatgcc acaagaaaaa ataagcataa aaaatacaca aaaaggggga cagtatacaa
     4141 gctgttatga ttttttttct gtctgttatt ttgggggttc ttgtgtgttt gaatagcatg
     4201 atgaaagcta taaatgctaa taatggtgcg ctcttaccgt agatatccat cacctttgtc
     4261 atcacagggt tatatgaaga aattactgaa ggtatatttt gagtattatc gaaaatgatg
     4321 cggattaaat agaagttggg gtttgatgta taagttataa aaataataat taatgggatt
     4381 atacccgcga aagtaatcaa aaaaagcatc ctgtttaaat ctttgttgta agtgtactct
     4441 ttttgcattg gtataatact cccggttttt cattgttatt aatttagttg ttgatgtgta
     4501 taagcatatc atgctgtgcg agtaatggtc accctaatat taggggtatg agttaatgag
     4561 gggatatatg aaaatgatat tatcaggagg gatgcttgtc ttgatggctt tgctatcagg
     4621 ctgccaggct aattatattc gcgatgttca gggagggacc gtcgcaccat cctcctcttc
     4681 tgaactgacg ggaatcgcgg ttcagtagaa aagatcaaag gatcttcttg agatcctttt
     4741 tttctgcgcg taatcttctg cttgcaaaca aaaaaaccac cgctaccaac ggtggtttgt
     4801 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttgga ggagcacagt
     4861 caccaaaacc tgtcttttca gtttagcctt aaccggctca taacttcaag accaactcct
     4921 ctaaatcagt taccagtggc tgctgccagt ggcgctttgt cgtgtcttac cgggttggac
     4981 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5041 cagcccagct tggagcgaac gacctacacc gaactgagat accaacagcg tgagctatga
     5101 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaaa cggcagggtc
     5161 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5221 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5281 agcctatgga aaaacgccag cggcgaggcc tttcgttgcc ctttgccttt ttgctcacat
     5341 gttctttccg gccttatccc ctgattctgt ggataaccgt attactgcct ttgagtgagc
     5401 ttgcaccgct cgccgcagcc gaacgaccga gcgtagcgag tcagtgagcg aggaagcgga
     5461 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgtatatg
     5521 gcgctgtttt cttacaatcc gcactgatgc tgcatatcta agccagtata cactccgcta
     5581 tcgctacgtg actgggtcag ggctgcgccc cgacacccgc taaaacctgc tgacgcgccc
     5641 tgacgggctt gtcggctccc ggcatccgct cacagacaag ctgtgtccgt ctccgggagc
     5701 tgcatgtgtc agaggttttc accgtcatcc ccgaaacgtg cgaggcagct gcggtaaagc
     5761 tcatcggcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagttcg
     5821 ttgagctttt ccagcagcgt taatgtctgg cttctgataa agcgcgccat gttaagggcg
     5881 gttttttcct gtttagtcac tgatgcctcc gtgtaagggg ggatttctgt tcatggggta
     5941 atgataccga tgaaacgcga gaggatgctc acaatacggg ttactgatga tgaacatgcc
     6001 cggttacttg aacgctgtga gggtaaacaa ctggcggtat ggatgcggcg ggtctgcctg
     6061 ggggagccgc ttgcccgttc cgggaaactg ccgacactgg caccgccgtt actgcgtcag
     6121 ctggccgcca tcggaaataa cctgaatcag acagcccgta aggtgaacag cgggcagtgg
     6181 tcttccggtg atcgggttca ggtggtggcc gcactgatgg ccatcgggga tgagctgcgc
     6241 cggctgcgtc tggccgtcag ggaacagggg gcgcgggatg atagttaaat ttcatgccag
     6301 gggaaaaggt ggtggcagtg gtccggttga ttacctgctg gggagggagc gtaaccgcga
     6361 aggcgcaacg gtgcttcagg gtaatccgga agaagtccgg gaactcatcg atgccacgcc
     6421 atttgcgaag aaatacacgt caggcgttct gtcgttcgcg gagaaggagc tgccgccggg
     6481 aggacgtgaa aaagtgatgg cgagctttga gcgcgtactg atgccaggtc tcgaaaagaa
     6541 tcagtacagc atcctgtggg tggagcacca ggacaaggga cggcttgagc tgaattttgt
     6601 cattccgaac atggagctac agaccggaaa acgtctccag ccgtactacg accgggcaga
     6661 caggccgaga attgatgcct ggcagacgct ggtgaatcac cattacgggc tgcatgaccc
     6721 caacgccccg gagaaccgcc ggatactgac tctctctgat aacctgccgg aaacgaaaca
     6781 ggcgcttgct gagagcgtca cacgcgggat agacgctctt tatcatgtag gggagataaa
     6841 aggccgtcag gatgtgattc aggcgctcac agaggcgggg cttgaagttg tcagggtgac
     6901 gcgaagcagc atcagcattg cagacccgaa cggcgggaag aatatcaggc tgaaaggagc
     6961 attttatgag caatcttttg cagacgggcg cggagttcga gaaaaagctg aaagagagag
     7021 ccgaatctac agagaaagtg ctgaacaacg agttcaggag gctcggcgaa tctgtaagcg
     7081 aggctgtgac atcaaacgag acgaaaatca gagacgctat agccctgttc acagcctcga
     7141 cagaggaatc gctggagaaa caccgggaag gggtgaaaga ggcgatgagg cagcacag
//
