LOCUS       CP057538                4721 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB23-C07 plasmid pRHB23-C07_4, complete
            sequence.
ACCESSION   CP057538
VERSION     CP057538.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148214
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH12 T2-C07
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 121.801822132294x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 09:36:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,811
            CDSs (total)                      :: 4,691
            Genes (coding)                    :: 4,574
            CDSs (with protein)               :: 4,574
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 53 of 117
            Pseudo Genes (incomplete)         :: 46 of 117
            Pseudo Genes (internal stop)      :: 37 of 117
            Pseudo Genes (multiple problems)  :: 19 of 117
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB23-C07"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB23-C07_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(4714..4721,1..859))
                     /locus_tag="HVX97_24040"
     CDS             complement(join(4714..4721,1..859))
                     /locus_tag="HVX97_24040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="QMJ48941.1"
                     /translation="MEKEINNWIEESGGKYRLMRQAVHIILKSISIEESLNEMMVMKGG
                     TLLGVKHGSDRYTKDIDFSTEKKLSEIDLGDFFNRFNDSMDVAEAELGYGIKCKVQSHK
                     IQPNAQGTFPTVQINIAFCDRSNASAMKRLESNQSPSVINIDFSFNEKTYDFDFLSLDG
                     DDDNDSVKTYSLTDLMAEKYRSVLQQKVRERNREQDIYDINYLLGKYEFSRQDKYKILE
                     SLLKKSEGKQLDNLLNVKGIRDVEIIERSSQRYQDLSSTVKSLPLFEDAYEKVACFYES
                     LPWDIFK"
                     /name="nucleotidyl transferase AbiEii/AbiGii toxin family
                     protein"
     gene            complement(843..1799)
                     /locus_tag="HVX97_24045"
     CDS             complement(843..1799)
                     /locus_tag="HVX97_24045"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMJ48938.1"
                     /translation="MYLIEEALTSWLLNEYKKPVITKEEISLQIYLFYLKKEYNGERIY
                     QIRKNKAGPNEFEKNILKLLKSGVLSRVESNDKHHAYPHLIGGFYEVFFVKGNDGSPEN
                     DIVCSTYPYGYLSYLSAMDWHNITDKIPKIIHFTTCRSEQWKNKFYHDFQKDNPDIDEA
                     KQFMPRFPRNSEVNGIKIIVTQETNYVEPMKARNSPVRVSSIGKTFMDMTRRPDYCGGE
                     EHVLESFIEHGKKYAPLIIKEVDKLGRHIDKVRVGFILDRIIGVNNKKISEWKQEAGKY
                     RGSSKVLSPKSPFSPYFDEEWFISINVDMVKSYGKRN"
                     /name="hypothetical protein"
     gene            2880..3074
                     /locus_tag="HVX97_24050"
     CDS             2880..3074
                     /locus_tag="HVX97_24050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMJ48939.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4064..4717)
                     /locus_tag="HVX97_24055"
     CDS             complement(4064..4717)
                     /locus_tag="HVX97_24055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C family protein"
                     /protein_id="QMJ48940.1"
                     /translation="MIAFSFSDIGNRCENLDLLSTINCQNDVFGMVIMDGYSCSEWNMK
                     ILSGRLDKCVFSGDLNKDIIEPCKSVPVKAAVCFLFKNNTHLHIYNAGDCRVYNKSGVL
                     LTEDNSVAWKRLKENGIDNKKIPHLVCYCPDRNYLTSFVDFCSDEIIFNHSVFDLNDEN
                     KEVILCTDGFWEHFNDGTVIDLMKDNPESNLAKFTKNIPGNAENHTVCYVRFPS"
                     /name="protein phosphatase 2C family protein"
     Typing          complement(2361..2616)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.512"
                     /coverage="89.0"
                     /evalue="1.21e-116"
     Typing          complement(2741..2844)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.286"
                     /coverage="100.0"
                     /evalue="4.87e-36"
     Typing          complement(3148..3230)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.79e-36"
     Typing          3243..3801
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.484"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atatatccca cggaagactt tcatagaaac aggcaacttt ttcataagca tcttcaaaaa
       61 gaggtaatga tttaacggtg gatgataaat cctgatatcg ctgactggaa cgctcgataa
      121 tttctacatc ccttatacct ttaacgttta agagattatc caattgttta ccttcactct
      181 tttttaaaag agactccagt attttatact tatcttgccg agaaaactca tatttaccta
      241 aaagataatt gatatcatat atatcttgct ctcgatttct ctctctaact ttttgttgca
      301 aaacggatcg atatttttca gccatcaaat cagttaagct gtaggtcttt acgctatcat
      361 tgtcatcatc accatctaat gataaaaagt caaaatcata cgttttttcg ttaaagctaa
      421 aatcgatatt tatcactgaa ggtgattgat tagattcaag ccttttcatt gcagacgcat
      481 tagaacgatc gcaaaaggct atatttatct gtacagttgg aaatgttcct tgtgcattag
      541 gttgaatttt atgagattga actttacatt taataccata tcccaattca gcctcagcaa
      601 catccattga gtcattaaac ctattgaaaa aatcccccaa gtcaatttca gaaagttttt
      661 tttcggtaga aaaatcaata tcctttgtgt atctatcact accatgctta acaccaagaa
      721 gagtccctcc cttcataacc atcatttcat tcaaactctc ctctatagaa atagatttaa
      781 gtattatatg tacagcctgc cgcatcaacc tgtacttccc accagactct tcgatccaat
      841 tattaatttc tttttccata agacttaacc atatcaacat taatagaaat aaaccattct
      901 tcatcaaagt aaggtgagaa tggactcttt ggagatagca ctttcgaact acctctgtat
      961 ttacccgcct cttgtttcca ttcgcttatt tttttattat taaccccaat aattctatcc
     1021 aaaatgaatc caacccttac cttgtcaata tgacggccaa gcttatcgac ttcctttata
     1081 atcagaggtg catatttttt accgtgctca ataaatgatt ccaatacatg ctcctcacca
     1141 ccacagtaat caggccgcct ggtcatatcc ataaaggtct taccgataga tgataccctg
     1201 acaggggaat tacgagcctt cattggctca acataatttg tttcttgtgt aacgataatt
     1261 tttattccat tgacctcaga atttcttgga aatctaggca tgaattgttt ggcttcgtct
     1321 atatcaggat tatccttttg aaaatcatga tagaacttat tcttccactg ttcggaacga
     1381 catgtcgtaa agtggattat cttgggtatt ttatctgtga tgttatgcca atccatagcg
     1441 gataaatagg ataagtatcc atatggatac gtagagcaca ctatatcatt ttccggagaa
     1501 ccatcgttac ctttaacaaa aaagacctca tagaaaccgc caattaaatg ggggtaagca
     1561 tgatgcttat cgttactttc aactcttgat aaaacccctg actttaataa ttttaatata
     1621 tttttttcaa attcattagg tcctgctttg ttctttctaa tttgatatat cctctcacca
     1681 ttatattctt tttttaaata aaaaaggtaa atctgcaaag agatttcttc tttggttatg
     1741 actggttttt tatattcatt caacaaccaa gacgttagcg cttcttcgat aagatacata
     1801 ttcaaccttt tcggtttagt ctctaataga atatacgtcc taaggacgta tattctatta
     1861 gagattgatt gtcaagaaaa aaataataac aaacaagcat ttgccatttt tatatttttg
     1921 atgtcctata aaataaatcc taaggattta ttttatagga taataagtag gaagtcaaga
     1981 aaaagtttac ttatatcacc gttttcataa cacctcatgc gatcaattca gcggcggtta
     2041 tagttcaaat ccatgattag gacatctgtt gctcataaac gatgagcgta aaagatcatt
     2101 tttcagcgtc atttaccata acgtcgcatt acgcgcaccg ctaaaacgcg ctgaacgcga
     2161 ttctgccgcg aacaccgcaa aaagagatgt tttaccaagc gaaaaccgga aaaatgcgtc
     2221 aggatcgctt tcagctcact gcatagctat gcatgaaagt gaatggcgat cggtttgggc
     2281 gcatacggtg cccatgagac acattttcgg acattttcag ttcgtgaatc cctgactcat
     2341 tgctacatat ctgaacagac acccctagca gcgcccccag gggcgtcctg taaaaaaaca
     2401 caccgcgccg ctagcagcac ccctaatata aaatgatgtt ttttatgaaa atagtcagta
     2461 ccacccctaa aaagcggtgt tggcgcagct acaacaccgc gccgacaccg cttttttaaa
     2521 tatcacaaag agagcaagaa gaactaattt gtcataactc tctatttata aagaaaaatc
     2581 agcaaaaaat tgtttttgcg tggggtgtgg gcatttcagg catgagaaca gactcgcgtt
     2641 tttccggagt atcagcggaa ttattgatta agaaacactc gacgcagagc gaaattttat
     2701 gcaacagcgg ctgcactgca cagggattat ttatcagttg atatgcggtg ccatgatacg
     2761 tcctaaaaaa acaggacagg acaggatgtg caattgtaat accgtcgccc gcaacgtctt
     2821 acaattacca cctggtcagg gtccacccga cacccggtac tgacggagaa caacggaagg
     2881 tgaacaaaca gcaacaaact gcgctgaata tggcgcgatt tatcaaaagc cagagcctga
     2941 cactgcttga aaaactggat gctctggatg ccgacgaaca ggcggccatg tgcgaacgac
     3001 tgcacgaact cgcggaagaa ctccagaaca gcatccaggt tcgctttgag accgaaagtg
     3061 aaacaggaac ataacgaagc tgacggaggc gctcacggct tgtccgcgag cgttttcggc
     3121 agcagacaag cccgccaggg cgcgtcagcg ggtgtcgggg cgaagccctg acccagtcac
     3181 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagtgcaga ttgtatgaaa
     3241 agtgccccat gccgggtgtg aaattccgca cagatgcgta aggagaaaat gcgcgtccag
     3301 gcgcttttcc gcttcctcgc tcactgactc gctacgctcg gtcgttcggc tgcggcgagc
     3361 ggtactgact tacacaaaaa cggcagcacg gttatccaca gaatcagggg ataaggccgg
     3421 aaagaacatg tgagcaaaaa ggcaaagaac aacaaaaggc cgcgccgctg gcgtttttcc
     3481 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3541 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3601 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3661 cgctttctca tagctcacgc tgttggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3721 tgggctgtgt gcacgaaccc cccgttcagc ccgactgctg cgccttatcc ggtaactatc
     3781 gtcttgagtc caacccggta agacacgcct taacgccact ggcagcagcc actggtaact
     3841 gatttagagg agttagtctt gaagtcatgc gccagttaag gctaaactga aagaacaggt
     3901 tttggtgact gcgctcctcc aagccagtta ccttggttct tcgagccgca aactctttga
     3961 acctaagaga aaaccgcccc gcaaaggcgg tttttttctt ttacagaaaa gagattatct
     4021 atatgacacg aagatctcaa tagcttcttc aaaaaacacg aaatcaagaa gggaatctaa
     4081 cgtagcaaac cgtatgattc tctgcgttac ccgggatatt tttagtaaat ttagccaagt
     4141 tagattctgg gttatctttc atgaggtcaa taacagtacc atcattaaaa tgctcccaaa
     4201 acccatcagt acataaaata acctctttgt tttcatcatt taaatcaaaa actgaatgat
     4261 tgaaaattat ctcatcagag cagaaatcaa caaatgatgt tagataattt ctgtctgggc
     4321 aataacaaac gaggtgaggt atttttttat tatcgattcc attttccttt aatcttttcc
     4381 aggcaaccga gttatcctct gttagaagga ctccactttt gttatataca cggcaatctc
     4441 ctgcgttata tatatggagg tgcgtattat ttttaaaaag gaaacatact gcagccttaa
     4501 caggaactga cttgcatggt tcaataatat ctttattcag atcaccagaa aacacgcact
     4561 tatctaacct accagagaga attttcatat tccactcaga gcaggaatac ccatccataa
     4621 taaccattcc aaacacatca ttttgacaat ttatcgtaga taataagtct aagttttcac
     4681 atctgttacc aatatcacta aaactgaacg ctatcatttg a
//
