LOCUS       CP057537                6930 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB23-C07 plasmid pRHB23-C07_3, complete
            sequence.
ACCESSION   CP057537
VERSION     CP057537.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148214
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH12 T2-C07
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 121.801822132294x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 09:36:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,811
            CDSs (total)                      :: 4,691
            Genes (coding)                    :: 4,574
            CDSs (with protein)               :: 4,574
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 53 of 117
            Pseudo Genes (incomplete)         :: 46 of 117
            Pseudo Genes (internal stop)      :: 37 of 117
            Pseudo Genes (multiple problems)  :: 19 of 117
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB23-C07"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB23-C07_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(6483..6930,1..1052))
                     /gene="csa"
                     /locus_tag="HVX97_23985"
     CDS             complement(join(6483..6930,1..1052))
                     /gene="csa"
                     /locus_tag="HVX97_23985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /protein_id="QMJ48936.1"
                     /translation="MAKELSGYGPTAGESVGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGGEHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPGAGGNSDVQG
                     GYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEK
                     AKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDNLPPNVRKFDVDVEQF
                     HYLVVLDDYGNVLSITRTGVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEEPQRQ
                     NQAKKAAYDVFYSFSMNRDRIQSDVLNKAAEVISDIGNKVGDYLGNAYKSLAREIADDV
                     KNFQGKTIRSYDDAMASLNKVLSNPGFKFNRADSDALANAWRSIDAQDMANKLGNISKA
                     FKFADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASAVALGVFSATLGAYAL
                     SLGAPAIAVGIVGILLAAVVGALIDDKFADALNNEIIKPAH"
                     /name="csa"
     gene            1293..1583
                     /locus_tag="HVX97_23990"
     CDS             1293..1583
                     /locus_tag="HVX97_23990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMJ48928.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            complement(1575..1853)
                     /locus_tag="HVX97_23995"
     CDS             complement(1575..1853)
                     /locus_tag="HVX97_23995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMJ48929.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLASLERQKKSLR"
                     /name="hypothetical protein"
     gene            1975..2397
                     /gene="exc2"
                     /locus_tag="HVX97_24000"
     CDS             1975..2397
                     /gene="exc2"
                     /locus_tag="HVX97_24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMJ48930.1"
                     /translation="MRSAAVRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFHTQKADTIRMMHPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEEFLQSLRTTTT
                     FAGRKYTNSDMPSPEKMKLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(2717..2950)
                     /gene="mbeD"
                     /locus_tag="HVX97_24005"
     CDS             complement(2717..2950)
                     /gene="mbeD"
                     /locus_tag="HVX97_24005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMJ48931.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="mbeD"
     gene            complement(2957..3475)
                     /locus_tag="HVX97_24010"
     CDS             complement(2957..3475)
                     /locus_tag="HVX97_24010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QMJ48932.1"
                     /translation="MSNLLQTGVEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIKGAIAQFTASTEESLKKHREGVKEAMMQHRKDMLKLAGNTGMRLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVLPYGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            complement(3453..4163)
                     /locus_tag="HVX97_24015"
                     /pseudo=""
     CDS             complement(3453..4163)
                     /locus_tag="HVX97_24015"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4153..4476)
                     /locus_tag="HVX97_24020"
     CDS             complement(4153..4476)
                     /locus_tag="HVX97_24020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMJ48937.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4541..4732
                     /locus_tag="HVX97_24025"
     CDS             4541..4732
                     /locus_tag="HVX97_24025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMJ48933.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5731..5886)
                     /gene="csl"
                     /locus_tag="HVX97_24030"
     CDS             complement(5731..5886)
                     /gene="csl"
                     /locus_tag="HVX97_24030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="QMJ48934.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GSSGSL"
                     /name="csl"
     gene            5916..6455
                     /gene="csi"
                     /locus_tag="HVX97_24035"
     CDS             5916..6455
                     /gene="csi"
                     /locus_tag="HVX97_24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 immunity protein"
                     /protein_id="QMJ48935.1"
                     /translation="MMIDEHSIDIDNRKANNLLYLFMVIGVIPLLCILAVYYTNPDNLF
                     LHTIATSTENIPSITSAYNPLMTKVMDIYCKTAPSLALILFILTFKTRKLIKKTNRNAV
                     LRSCLLSPFVCAVFLYLLCFRNLELTTAGRPARLMANNDATLLLFYIGLYLIIFFISYF
                     TLFTPVTTFKLLKERQ"
                     /name="csi"
     Typing          complement(4818..4905)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          4928..5474
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.171"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2614..4163)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.603"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tagctacgga tggttttccc ctggaaattt ttgacatcat cagcaatttc tcttgctaac
       61 gacttataag catttccaag ataatcaccg accttattac ctatatcaga gataacttca
      121 gcagctttat taagtacatc actctgaatc cggtcacggt tcatactgaa agaataaaac
      181 acatcataag cagctttttt agcttgattc tgtcgttgag gctcttcatt attaaatcct
      241 aatgcctcat atatctcttc cggggtctta tggtcgactt tatccataat accagcctta
      301 gctttttcac tccctacata aggacgaaca cctgttctag ttatagataa aacattacca
      361 taatcatcaa gcaccactaa atagtggaac tgttcaacat caacatcaaa ttttctaaca
      421 ttaggaggca aattatccca cgcatcttca gcctgttgtt ttgctttttc ctgccgctga
      481 gattcatctt tattaaaacc taacgcctca tatatctcct caggtgtttt atgattaatt
      541 ttttccataa tacccgcctt agctttttca ctccctacgt aaggacggac acctgttcga
      601 agaataccga tagctctacc attatcatta agcgttacac gatatttaaa accatcgata
      661 ctcacatcaa tctctcgcgg taagttgttt ttatcccact tacgagcaac ttcatcatta
      721 ggattttcag gaatatagcc cccctgaaca tcactattcc cgccagcccc aggaactcca
      781 tatacaggtg tttttaccag accatttgtg atggtaatgc gcacctgccc attatcatca
      841 acataaggcg gcacatccgg attattggtt ttactccatc cccaccccgt ctgacttcca
      901 tgttcaccac ctccattacc agagcctgaa ccaccacccc agtgaacccc actattacta
      961 ttattatttc caccttgctg attcagattc gcccctgttc cccccacaga ctcaccagca
     1021 gttgggccat atccacttag ttctttagcc ataaattcct ctttgataat taaaacaata
     1081 aattaaaagc aatatactgt acatataacc actggttttg tattcagcaa aaacctacta
     1141 ctcagcatta tccatgtcaa gagcatggat tttcattttt gcaataagga tcacactatg
     1201 gggaggcaga cattgagaac gtcgaaacag aacaccggag caaatcagga tgagatataa
     1261 aactgttgga tcatgaaaaa acggagaacg atgtgagcaa atctccccgc cataaactga
     1321 acaaaacaga caagcgactt ctcgacaccc tggttgctgc cggatatgag catgacaaag
     1381 cccgtgacct catccagaaa caggtttaca cgctgacact ggctgatcag cgtcatgtgg
     1441 tcagtgaaat cagtaacggt gtgaatccca cccaggctta ctcggcggta taccaggcaa
     1501 gacgcattcg cctcgcccgt aaatatctga acggtaaaaa ggttatggaa gaaaccgggg
     1561 aaaatacgcc cccatcagcg taaggatttc ttttgccgct ccagagacgc cagtttttta
     1621 cgcaaatcct ctcttttttg ggcatctctg gtgccagcca gctctgctct caactcatcg
     1681 atctgaagtt gtatcttcag tctgttactg aacattttct gtctggcatt aacatccgca
     1741 acaatgccgt tttttgtctt ctcggccttt tgttgaaaaa cattgctgtc cgcatgactg
     1801 gcaaccgaag cagaaagaac actaaaaagc aggactggca cacatttttt cacgggatta
     1861 ttcctgactc attgaccatc aagtcacatt gggagtaaac cgacgtatga taagagatac
     1921 tcttcggaga tataactccc tgagtatcaa gattaaaaac gcaaggagat gtttatgaga
     1981 tctgccgctg tcaggctgct tctgatacct ctgataacag caacaatagc tcttacagga
     2041 tgcacaccaa agaccagcct ggaacgacat acccggcatt atgtttatgc ttcagatgat
     2101 ggatttgatc ctaacttcca cacccagaaa gcagacacca tacgtatgat gcacccgttc
     2161 tttcagcagt tccgggatat ggggatgaaa gacaaagcag ccggagtatc agcagaaacg
     2221 gcacagcaac gtgtaaaaga attccactct gaagaattcc ttcagtcact gagaaccaca
     2281 accacctttg cgggcagaaa atacacgaac agtgatatgc catcaccgga aaaaatgaag
     2341 ctgctggctg acacgatttc tgcggtttat ctcgatggct acgagggcag gcagtaaggg
     2401 agacatttac cataatccct tattgtacgc accgctgaaa cgcgttcagc gcgatcacgg
     2461 cagcagacag gtaaaaatgg caacaaacca cccgaaaaac tgccgcgatc gcgcctgata
     2521 aattttaacc gcatgaatac ctatgcaacc agatggtaca ggccacatta acccctctta
     2581 atccactgaa gctgccattt ttcatggttt caccatccca gcgaaggacc atccagcgtg
     2641 cgttcctgta tttccggctg acgctcccgt tccagggata acgcatgttc gcgttcctgt
     2701 atcagccgtt cgtcccttat ctcctgttct cgctgtataa ctggctcaag cgttctgtcc
     2761 gctcgctcaa gtgctgcacc tgctgactca actgcatgac ctgctcgttc agcatcgcgt
     2821 tgtcccgttg cgtaagcgaa aacattttct gcaattccac gaaggcgctc tcccattcgc
     2881 tcagccgctg catatagtcc tgttgcagtt gctctaatgc gttcagcaaa tgtctttcca
     2941 gctccgtcac tctgtgtcac tccgtcagtt gtacccagtc cttcccctga tagggaatca
     3001 ccgttgccga tttcccgtac ggaaggacca gaaatttcct tctcccgtcc tgcacaaact
     3061 ccacgcccca tgtcttcgcg ttcagtttct gcaatgtctc ttcctgtatc ctgatttctt
     3121 ccaggttcgc ctgtatccgc ccgccaagat accagagcgt cccgccactc gcggtaaaca
     3181 ggagaaagac tatccccagc aacctcatgc ccgtattccc tgccagcttt aacatgtcct
     3241 tccggtgctg catcatcgcc tctttcaccc cttcccggtg ttttttcagc gattcctccg
     3301 tcgaggccgt aaactgggct atagcgcctt tgattttcgt ctcgtttgag gtcacagcct
     3361 cgcttacaga ttcgccgagc ttcctgaatt cgtcgttcag cattttctct gtagattcgg
     3421 ctctctcttt cagctttttc tcgaactcca cgcccgtctg taaaagattg ctcataaaat
     3481 gctcctttca gcctgatatt cttcccgccg ttcgggtctg caatgctgat actgcttcgc
     3541 gtcaccctga ccacttcaag ccctgcctct gtgagcgcct gaatcacatc ctgacggcct
     3601 cttatctccc cgacatgata aagagcgtct atcccgcgtg tgacgctctc tgcaagcgcc
     3661 tgtttcgttt ccggcaggtt atcagggagt gtcagtatcc ggcggttctc cggggcgttg
     3721 gggtcatgca gcccgtaatg gtgattcacc agcgtctgcc aggcatcaat tctcggcctg
     3781 tctgcccggt cgtagtacgg ctggaggcgt tttccggtct gtagctccat gttcggaatg
     3841 acaaaattca gctcaagccg tcccttgtcc tggtgctcca cccacaggat gctgtactga
     3901 ttcttttcga gaccgggcat cagtacgcgc tcaaagctcg ccatcacttt ttcacgtcct
     3961 cccggcggca gctccttctc cgcgaacgac agaacgcctg acgtgtattt cttcgcaaat
     4021 ggcgtggcat cgatgagttc ccggacttct tccggattac cctgaagcac cgttgcgcct
     4081 tcgcggttac gctccctccc cagcaggtaa tcaaccggac cactgccacc accttttccc
     4141 ctggcatgaa atttaactat catcccgcgt cccctgttcc ctgacggcca gacgcagccg
     4201 gcgcagctcg tccccgatgg ccatcagtgc ggccaccacc tgaacccggt caccggaaga
     4261 ccactgcccg ctgttcacct tacgggctgt ctgattcagg ttatttccga tggcggccag
     4321 ctgacgcagt aacggcggtg ccagtgtcgg cagtttcccg gaacgggcaa ccggctcacc
     4381 cagacagacc cgccgcatcc ataccgccag ttgtttaccc tcacagcgtt ccagtaaccg
     4441 ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc tcgcgtttca tcggtatcat
     4501 tacccccatg aacagaaatc ccccttacac ggaggcatca gtgactaaac aggaaaaaac
     4561 cgcccttaac atggcccgct ttatcagaag ccagacatta acgctgctgg aaaagctcaa
     4621 cgaactggac gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgccgatga
     4681 gctttaccgc agctgcctcg cacgtttcgg ggatgacggt gaaaacctct gacacatgca
     4741 gctcccggag acggtcacag cttgcctgtg agcggatgcc gggagcagac aagcccgtca
     4801 gggcgcgtca gcaggtttta gcgggtgtcg gggcgcagcc ctgacccagt cacgtagcga
     4861 tagcggagtg tatactggct taactatgcg gcatcgatgc ggattgtatg aaaagtgcgc
     4921 catggacggt gtgaaatgcc gcacagatgc gtaaggagaa aatgcaactt acgacactct
     4981 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtgccg
     5041 gctcactaaa aggcggaaat acggttatcc acagaatcag gggataaggc cggaaagaac
     5101 atgtgagtaa aaaggcaaag gacaaaaaaa ggccgcgccg ctggcgtttt tccataggct
     5161 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     5221 aggactataa agataccagg cgtttccccc cggaagctcc ctcgtgcgct ctcctgttcc
     5281 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     5341 tcatagctca cgctgttggt atctcggccc ggtgtaggtc gttcgctcca agctgggctg
     5401 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtctcga
     5461 gtccaacccg gtaagacacg acaaagcgcc actggcagca gccattggta actgaaaagt
     5521 ggatttagat acgcagaact cttgaagttg aagccttata gcggctacac tgaaaggaca
     5581 gcatttggta tctgtgctcc acttaagcca gctacccggt taagcagtcc ccaactgact
     5641 taaccttcga ctaaaccgcc tccccaggcg gttttttcgt ttacagggca agagactacg
     5701 acgatctgta aaggatctca agaagatcct ttacaggcta ccactactac cacttcccat
     5761 actcactccg gtaacagtgg aagatggtga aacagcccca cctccagtat ctctgacatt
     5821 gttgacttgg catgctgcaa gcagcattat tcctagtgaa atggcacaaa taataatttt
     5881 tttcatatat acctttatta taatcctata ggtcaatgat gattgatgaa cattcaatag
     5941 atatagacaa tagaaaagcc aataacttat tgtatttatt tatggtaatc ggagttatac
     6001 ctttactctg tatactagcc gtttactata caaatccaga caatctgttc ttacacacaa
     6061 tagctaccag tactgaaaac atcccatcaa taacctccgc ctacaaccca ttaatgacaa
     6121 aggttatgga tatttattgt aaaacagcgc cttcccttgc tttgatatta tttatcctga
     6181 catttaaaac gagaaaacta ataaaaaaaa caaaccgaaa tgctgtactt agaagttgct
     6241 tattaagtcc atttgtctgt gctgtttttt tgtatttact ctgcttccga aaccttgagc
     6301 taacgacagc aggaaggcca gctaggctaa tggccaacaa tgatgcaaca ctattgctat
     6361 tttatattgg cctgtactta atcatttttt ttataagcta tttcacgcta ttcacaccag
     6421 ttaccacgtt taaattatta aaagaaaggc agtaaccctg ccttcctttt acccggtatt
     6481 tattaatgag cgggtttaat gatttcgtta tttagtgcat ctgcaaactt gtcatcaatt
     6541 aatgcaccta caactgctgc gagtagaata cctacgatac cgacagcaat agcaggagcc
     6601 ccaagagata aggcatatgc ccctaatgtg gctgaaaaaa cccccagggc cacagcagaa
     6661 gcaatgccac tgagcaccca ggactccacc tccagcatca gaggtcccca gttcccggtt
     6721 tcatagccct cgatgctttt ctcccggacc ttctcaacct tcatcaccac atcggcaaat
     6781 ttgaaagcct tgctgatatt acccagttta tttgccatat cctgagcatc aatacttcgc
     6841 caggcgttcg ccagtgcatc actgtcggcc ctgttaaatt taaacccagg attagacaaa
     6901 actttattta atgacgccat tgcatcatcg
//
