LOCUS       CP057533                5674 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB23-C10 plasmid pRHB23-C10_9, complete
            sequence.
ACCESSION   CP057533
VERSION     CP057533.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148215
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 5674)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH12 T2-C10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 121.496788627939x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 09:29:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,672
            Genes (coding)                    :: 4,533
            CDSs (with protein)               :: 4,533
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 54 of 139
            Pseudo Genes (incomplete)         :: 71 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 20 of 139
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB23-C10"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB23-C10_9"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(5659..5674,1..911)
                     /locus_tag="HVX98_23920"
     CDS             join(5659..5674,1..911)
                     /locus_tag="HVX98_23920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QMJ44357.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKEHELVRMLQHDVAREHGFD
                     MRDRVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            914..1447
                     /locus_tag="HVX98_23925"
     CDS             914..1447
                     /locus_tag="HVX98_23925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMJ44358.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVTLED
                     KLAEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            1669..2793
                     /locus_tag="HVX98_23930"
     CDS             1669..2793
                     /locus_tag="HVX98_23930"
                     /inference="COORDINATES: protein motif:HMM:NF013211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMJ44359.1"
                     /translation="MSGGDGRGPGNTGLGHNGGKPSGNVNGSSGHGGPSSGGGYHYNPG
                     KGPASTVGPDGEVHINITGGMEKDPPGKHHGNGGSGNGHGAGNHNSNSGGNGKTTSIPG
                     WSPDSGKPGRYLPTNKDKLIKGLIQSIDDHSGTYTIHYTGGAVATVTVPNGDVNKMNVH
                     YNIGSGPSSGNKLTRDTIKSFLEFKAENEANAVNDAISKVNDFYKELASKYGDKLEKEA
                     KALADEAKGKKIRNANEAIKAFDRYKDVLNKKFSVSDREAIAKALESLNKDQMAKQLKI
                     FGKAFGVVGEAIQWGGFVSGLVKGFRTGDWNEAFISGEKIAVGKVASVMVTVAFSAMAV
                     NPIGILGFAVIMAVTSALITDERLKQLNSFINGI"
                     /name="hypothetical protein"
     gene            3594..4502
                     /locus_tag="HVX98_23935"
     CDS             3594..4502
                     /locus_tag="HVX98_23935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QMJ44360.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAIERALCEKLGADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASA
                     CRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPDYVRWLEAVNQRVEMRNASLPVPLSP
                     AECRAIGKSIAKYTHRKFSPEGFSAVQAARGRKGGIAKGEAYEDKRLMALCMLENGYSQ
                     KAIAAMLEVSTRTIRNWKSGK"
                     /name="replication initiation protein"
     gene            4727..5044
                     /locus_tag="HVX98_23940"
     CDS             4727..5044
                     /locus_tag="HVX98_23940"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMJ44361.1"
                     /translation="MPNLPKSVIAADICDSWVTRYSSHPMSKPRLVAFWKLMAKQFRVG
                     GRISRSEYRALKGHLAMAVVMVDSPSSFASPELRKAIVATALEAEEIIFIIEPDLKGFE
                     V"
                     /name="hypothetical protein"
     gene            complement(5090..5482)
                     /locus_tag="HVX98_23945"
     CDS             complement(5090..5482)
                     /locus_tag="HVX98_23945"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMJ44362.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAIQKRIEGKKQRRERNHRLFLAAGLLIEAGLMESKTG
                     NLKFKRELTLQALKRIRYELETSPEA"
                     /name="hypothetical protein"
     Typing          3595..4322
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.623"
                     /coverage="94.0"
                     /evalue="0.0"
     Typing          4349..4502
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.548"
                     /coverage="88.0"
                     /evalue="4.26e-58"
     Typing          4..908
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.682"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 tcagcgttaa atttggtggc aaggggcagg ctgcaaacca cgccgattac atcgagcgca
       61 aagagaaata ccgcgaccgt caggatttgg aatattccgc acacggaaac atgccggaat
      121 gggcgcgaga taatccctca catttctggc aggcggctga ccagtttgaa cgtgcgaacg
      181 gttcgaccta tcgggaactt gaaattgcat taccccgtga actgacagcg gaacagcgcc
      241 ttgaactggt gcaggatttt gtacgtcagg aagctggcga acggcacgcc tggagttttg
      301 ccattcacaa tccgaaagcg agcattgacg gcggcgaaca gcctcatgcg catatcatga
      361 tgagccagcg tgtgaatgat ggcattgaac gtacaccgga acagtatttc cggcgctata
      421 acgccaggta tcccgagcgg ggaggtgcga aaaaggattc cggaagcctg actctcactc
      481 agcaaaaaga gcagttgcgg gaattgcgaa aacgctggga ggtgaagcac aacgaacata
      541 tgagaaagca tggctttgaa aggggcttca ttgactgccg gacgctgaaa gaacagggca
      601 ttgaacgacg tcccgaagtg catctgggtt ttgaagctgc cggacggctt gatgatgccc
      661 agcgtcatga catcatgcaa aaacggtctg aaccggacta ttcccgccat cttgaacagg
      721 cgcgaacggt cgggcatgac atcagggaat ggctggacac tccggttaag gaacatgaac
      781 tggtcagaat gctgcaacat gatgttgcgc gggagcatgg ttttgacatg agagacagag
      841 tgagcagaca gaaccgaact gaacctgaac gggttaaatc accgtcacgt ggattttcta
      901 tgggattctg aacatggcca gcacagaaga aaaattactc acgctcctgt tatcggtgga
      961 gcatctgcaa cataccgcaa tggagcaaca gaaagctgtt gctgattctc tggctgtggc
     1021 aagggaaaca cttcgcggat atcgggatat ttctgacggt gtggcaaaac aggtcaggga
     1081 tgaagtcaga aacgaggtgc agaagctgga tgttgcaggg ctgataggtg agcgcatcag
     1141 ccaggatttt cagcggctgg aagagtcagt cgcaaacatg aaggcaaacg cggatgcctt
     1201 gaacagggat attgcgacaa ttcacagcaa tatcgtgcag gaatacaaca cccttaaagg
     1261 gtatagctgg aaatggctgg gtggggtgtc agtggctttt ctggttatgt tctgtgtgct
     1321 tggatgggga atgaaaacaa tgctcgacag gaactacaac gccacagcgg cggtgtatca
     1381 gaaagttgtg acactggaag ataaactggc ggagaaacct gtcacgggca agagccggaa
     1441 aaactgacgc gcttcgcttg tccaacgcct cacgacccgc gaaaggattt cgcgttccgt
     1501 tcaccgtttt catgagtgat tcctccgaac agccaaaaaa tatccctccc ctgacttgac
     1561 agaaaaaata atggcgagta ctttttgatc tgtacataaa accagtggtt ttatgtacag
     1621 tattaactat gtaatcaatt gttttaacgc ttaaaagagg gaatttttat gagtggtgga
     1681 gatggtcgtg gccctggtaa tacagggctt ggacataacg gtggtaaacc cagtggaaac
     1741 gtgaatggct catctggaca tggaggacca tcatctggtg gtgggtatca ttacaatccg
     1801 ggaaaaggtc ctgcaagcac tgtaggtcct gacggggagg tgcatatcaa cattaccggt
     1861 ggtatggaaa aagatcctcc aggaaaacat cacggtaatg gtggctcagg taatggtcac
     1921 ggtgctggaa atcataacag caattcaggc ggcaatggaa aaacaacgtc tattcctgga
     1981 tggagccctg actctgggaa acctggcaga tatttaccga caaataaaga taagttaatt
     2041 aagggactta ttcagtcaat agatgaccat tctggaacgt acactatcca ttatactggt
     2101 ggtgcagtag ctacagtaac tgtaccaaac ggtgatgtga ataaaatgaa tgttcactat
     2161 aatatcggta gtgggccttc atcaggtaat aaactgactc gtgatactat aaaaagcttt
     2221 ttagaattca aggcagaaaa tgaagctaat gctgtaaatg atgcaataag caaagttaat
     2281 gacttttata aagagttagc atcaaaatac ggcgataagt tggagaaaga agccaaggca
     2341 ctagccgatg aagctaaagg gaaaaaaatt cgcaatgcta atgaggcgat taaagccttt
     2401 gataggtaca aggatgtgct taataagaaa ttcagcgtat ccgaccgaga agcgattgca
     2461 aaagcactgg agtctcttaa taaagatcaa atggctaaac agttaaaaat ttttggtaag
     2521 gcctttggtg tcgtaggtga ggctattcag tgggggggat ttgtcagtgg cttagttaaa
     2581 ggattcagaa ctggtgactg gaatgaggct tttataagcg gggaaaaaat cgcagtcggc
     2641 aaggtagcat ctgttatggt tacagtggca tttagtgcta tggcagtaaa tcctattggt
     2701 attttagggt ttgcagtaat tatggcagtg actagtgcct taattacaga tgaaaggctg
     2761 aaacaactaa acagctttat taacggcatt taataaacaa aaggtcgtac cgtttgatat
     2821 ggtatgacct ttattttgtt tttctatcgc tttttgttgg ttataaagta atagactgta
     2881 aaaatcaatc caaaaggagg tagtgataat ataatgcata ttataaaacc ccaattaacg
     2941 aagttaaaag ttgtttttgt tagtaaggga gtttgcttat ttattagtaa tagacaaaaa
     3001 gggtatgtgg acgatccaaa aagagatata agtataaata taaataattc tatacttgtt
     3061 gggcttagat tcaaacttgc gatgataaaa gataagatgt aaaaggataa ggcaaggata
     3121 aaattcctta aatagaattc catatctatt tttttcattt ataaaatcct cgttagttaa
     3181 atgtttataa tattgcctta ctttatccat aaatctagaa gtagttatca caaaagttac
     3241 ttttttgact cgttcgccct tctgaacgtt cgaaactgtt tgcgaatcgc tttcgtgcat
     3301 agttatgcaa cgccctgaaa gtgattctga cgcatttttc tggtttggcc tggtgtttcc
     3361 ttgtcttttt gcgttttttg cgccagaaag cgactgaggg cgttttaagg ggtacgtaca
     3421 acgggagtta tggtaaatgg ctcggatttg cgggaaggct cgacaggttt tgtcgttggg
     3481 ggtagtgtaa gcgactgaaa aacaaacgcc cctgaaattc gtgtctctcc gccaagactt
     3541 tcacgaaaat acaggggcga tgtcggtcga taacctggaa ggaatcgtaa cacatgagcg
     3601 ccgcgcttca atacttcgaa gaaaatttac cccaccgtcc ctatcacacg gatgatctcg
     3661 ctttcggcct tcgtatctcc ggcaaagggc gcgcgcttct tgcacggtat atccagcaga
     3721 accagcctca tgcacagttc tggctggttt ttgacgttga ccgcgagggg gctgctattg
     3781 actggagcga ccggaacgcc cccgcgccga acatcaccgt aaaaaatcct gtgaacggtc
     3841 acgcccacct gctctacgca ctcaatatcg ccgtgagaac cgcgccagat gcctcggtta
     3901 aggcgctgaa atacgctgcc gcgatagagc gtgcgttgtg tgagaaactt ggcgcagatg
     3961 tgaattacag cggactgatt tgtaaaaatc cgttccacct ggaatggcag gtgatggagt
     4021 ggcgcgagga agcctatacc ctcgatgaac tggctgatta tctcgatttg agcgcctcag
     4081 cgtgccgtag catcgataaa cattacggga tggggcgaaa ctgccacctg ttcgaaatga
     4141 cgcgcaaatg ggcttacagg gcgattcggc agggctggcc cgattacgtt cgctggctgg
     4201 aagcggtgaa ccagcgtgtc gaaatgcgca acgcatcgct tcccgtaccg ctttctccgg
     4261 ctgaatgtcg ggctattggc aagagtattg cgaaatacac gcacaggaaa ttctcgccgg
     4321 aaggtttttc cgctgtacag gctgcacgag gccggaaagg tggcatcgca aaaggcgaag
     4381 cctacgagga caagcgctta atggcattgt gtatgctgga gaatggatat tctcagaaag
     4441 ctattgctgc gatgttggag gtttctactc gaactattcg aaactggaaa agcggaaaat
     4501 agccttatca gataacagcg cctttttggc gtttttttga gccgtaggtt ttccggcggt
     4561 tttctctgct cagccctatg atggtgcggc tttctgcatg attgaggtgg tagtgctcgc
     4621 cgcagcctca tgaccgagcg ttcgcgagcg aatgagcgag gaagcaccag ggaaccggaa
     4681 cctagtggtg gctctttcgc gctgtcgttt attttcaaga ggttatatgc ctaacttacc
     4741 caaaagtgtt attgctgccg acatctgcga ttcttgggtg actcggtatt cttctcatcc
     4801 catgagcaag ccgcgtcttg tcgctttctg gaaattgatg gctaaacagt ttcgtgtagg
     4861 tggccggatt agccgtagtg agtacagggc gttgaaaggc catcttgcta tggctgtcgt
     4921 catggttgat tcccctagtt catttgcatc tccggaacta cgaaaagcta ttgttgccac
     4981 agccttggaa gcggaggaga tcatttttat catcgaacct gacttaaagg gcttcgaggt
     5041 ttaaattggc tgtttactaa acggaaaaat ggtcctggaa tagggactct tacgcctctg
     5101 ggcttgtttc cagctcatac ctgatccgtt taagtgcctg taacgtaagc tctcttttga
     5161 acttcaggtt gcctgttttt gattccatta gtcccgcttc gatgagtaat cctgccgcca
     5221 gaaagagccg gtgattgcgt tcccgtcgtt gtttttttcc ctcgattcgt ttctggatcg
     5281 cctgttttcg ttcgctctcc tgtgcctgta aatcgttttt ttccgctgtt gccagtacgt
     5341 ggagaagttt gcgctcctgt tctgtgagtc cggcttcgtt gtcgagcagg tcaataatct
     5401 gtctctgtcg cgcggtttgt tctccgagct tcctgatgaa tccgatccgt ttcttcagcc
     5461 attcgatgtt gctgatgcgc atgttcgtgg tgctccctgg tgtttttttg cggtgtgttg
     5521 tggtcgtgca gtatatcgct tgcggtcggt gggtgcaggg ggtatcttcc gtgctaattc
     5581 gtaaaattag aaggaagcgc acttatatgt ttctctcctt cgtcgagaaa cagtgcgctc
     5641 ctgcggaggg ctgaaaccgt ggcaacctat cacc
//
