LOCUS       CP057530                6930 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB23-C10 plasmid pRHB23-C10_6, complete
            sequence.
ACCESSION   CP057530
VERSION     CP057530.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148215
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH12 T2-C10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 121.496788627939x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 09:29:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,672
            Genes (coding)                    :: 4,533
            CDSs (with protein)               :: 4,533
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 54 of 139
            Pseudo Genes (incomplete)         :: 71 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 20 of 139
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB23-C10"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB23-C10_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(5502..6930,1..71))
                     /gene="csa"
                     /locus_tag="HVX98_23820"
     CDS             complement(join(5502..6930,1..71))
                     /gene="csa"
                     /locus_tag="HVX98_23820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /protein_id="QMJ44348.1"
                     /translation="MAKELSGYGPTAGESVGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGGEHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPGAGGNSDVQG
                     GYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEK
                     AKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDNLPPNVRKFDVDVEQF
                     HYLVVLDDYGNVLSITRTGVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEEPQRQ
                     NQAKKAAYDVFYSFSMNRDRIQSDVLNKAAEVISDIGNKVGDYLGNAYKSLAREIADDV
                     KNFQGKTIRSYDDAMASLNKVLSNPGFKFNRADSDALANAWRSIDAQDMANKLGNISKA
                     FKFADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASAVALGVFSATLGAYAL
                     SLGAPAIAVGIVGILLAAVVGALIDDKFADALNNEIIKPAH"
                     /name="csa"
     gene            312..602
                     /locus_tag="HVX98_23825"
     CDS             312..602
                     /locus_tag="HVX98_23825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMJ44339.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            complement(594..872)
                     /locus_tag="HVX98_23830"
     CDS             complement(594..872)
                     /locus_tag="HVX98_23830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMJ44340.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLASLERQKKSLR"
                     /name="hypothetical protein"
     gene            994..1416
                     /gene="exc2"
                     /locus_tag="HVX98_23835"
     CDS             994..1416
                     /gene="exc2"
                     /locus_tag="HVX98_23835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMJ44341.1"
                     /translation="MRSAAVRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFHTQKADTIRMMHPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEEFLQSLRTTTT
                     FAGRKYTNSDMPSPEKMKLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(1736..1969)
                     /gene="mbeD"
                     /locus_tag="HVX98_23840"
     CDS             complement(1736..1969)
                     /gene="mbeD"
                     /locus_tag="HVX98_23840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMJ44342.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="mbeD"
     gene            complement(1976..2494)
                     /locus_tag="HVX98_23845"
     CDS             complement(1976..2494)
                     /locus_tag="HVX98_23845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QMJ44343.1"
                     /translation="MSNLLQTGVEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIKGAIAQFTASTEESLKKHREGVKEAMMQHRKDMLKLAGNTGMRLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVLPYGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            complement(2472..3182)
                     /locus_tag="HVX98_23850"
                     /pseudo=""
     CDS             complement(2472..3182)
                     /locus_tag="HVX98_23850"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3172..3495)
                     /locus_tag="HVX98_23855"
     CDS             complement(3172..3495)
                     /locus_tag="HVX98_23855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMJ44344.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3560..3751
                     /locus_tag="HVX98_23860"
     CDS             3560..3751
                     /locus_tag="HVX98_23860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMJ44345.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4750..4905)
                     /gene="csl"
                     /locus_tag="HVX98_23865"
     CDS             complement(4750..4905)
                     /gene="csl"
                     /locus_tag="HVX98_23865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="QMJ44346.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GSSGSL"
                     /name="csl"
     gene            4935..5474
                     /gene="csi"
                     /locus_tag="HVX98_23870"
     CDS             4935..5474
                     /gene="csi"
                     /locus_tag="HVX98_23870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 immunity protein"
                     /protein_id="QMJ44347.1"
                     /translation="MMIDEHSIDIDNRKANNLLYLFMVIGVIPLLCILAVYYTNPDNLF
                     LHTIATSTENIPSITSAYNPLMTKVMDIYCKTAPSLALILFILTFKTRKLIKKTNRNAV
                     LRSCLLSPFVCAVFLYLLCFRNLELTTAGRPARLMANNDATLLLFYIGLYLIIFFISYF
                     TLFTPVTTFKLLKERQ"
                     /name="csi"
     Typing          complement(3837..3924)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          3947..4493
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.989"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1633..3182)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.412"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttcagattcg cccctgttcc ccccacagac tcaccagcag ttgggccata tccacttagt
       61 tctttagcca taaattcctc tttgataatt aaaacaataa attaaaagca atatactgta
      121 catataacca ctggttttgt attcagcaaa aacctactac tcagcattat ccatgtcaag
      181 agcatggatt ttcatttttg caataaggat cacactatgg ggaggcagac attgagaacg
      241 tcgaaacaga acaccggagc aaatcaggat gagatataaa actgttggat catgaaaaaa
      301 cggagaacga tgtgagcaaa tctccccgcc ataaactgaa caaaacagac aagcgacttc
      361 tcgacaccct ggttgctgcc ggatatgagc atgacaaagc ccgtgacctc atccagaaac
      421 aggtttacac gctgacactg gctgatcagc gtcatgtggt cagtgaaatc agtaacggtg
      481 tgaatcccac ccaggcttac tcggcggtat accaggcaag acgcattcgc ctcgcccgta
      541 aatatctgaa cggtaaaaag gttatggaag aaaccgggga aaatacgccc ccatcagcgt
      601 aaggatttct tttgccgctc cagagacgcc agttttttac gcaaatcctc tcttttttgg
      661 gcatctctgg tgccagccag ctctgctctc aactcatcga tctgaagttg tatcttcagt
      721 ctgttactga acattttctg tctggcatta acatccgcaa caatgccgtt ttttgtcttc
      781 tcggcctttt gttgaaaaac attgctgtcc gcatgactgg caaccgaagc agaaagaaca
      841 ctaaaaagca ggactggcac acattttttc acgggattat tcctgactca ttgaccatca
      901 aatcacattg ggagtaaacc gacgtatgat aagagatact cttcggagat ataactccct
      961 gagtatcaag attaaaaacg caaggagatg tttatgagat ctgccgctgt caggctgctt
     1021 ctgatacctc tgataacagc aacaatagct cttacaggat gcacaccaaa gaccagcctg
     1081 gaacgacata cccggcatta tgtttatgct tcagatgatg gatttgatcc taacttccac
     1141 acccagaaag cagacaccat acgtatgatg cacccgttct ttcagcagtt ccgggatatg
     1201 gggatgaaag acaaagcagc cggagtatca gcagaaacgg cacagcaacg tgtaaaagaa
     1261 ttccactctg aagaattcct tcagtcactg agaaccacaa ccacctttgc gggcagaaaa
     1321 tacacgaaca gtgatatgcc atcaccggaa aaaatgaagc tgctggctga cacgatttct
     1381 gcggtttatc tcgatggcta cgagggcagg cagtaaggga gacatttacc ataatccctt
     1441 attgtacgca ccgctgaaac gcgttcagcg cgatcacggc agcagacagg taaaaatggc
     1501 aacaaaccac ccgaaaaact gccgcgatcg cgcctgataa attttaaccg catgaatacc
     1561 tatgcaacca gatggtacag gccacattaa cccctcttaa tccactgaag ctgccatttt
     1621 tcatggtttc accatcccag cgaaggacca tccagcgtgc gttcctgtat ttccggctga
     1681 cgctcccgtt ccagggataa cgcatgttcg cgttcctgta tcagccgttc gtcccttatc
     1741 tcctgttctc gctgtataac tggctcaagc gttctgtccg ctcgctcaag tgctgcacct
     1801 gctgactcaa ctgcatgacc tgctcgttca gcatcgcgtt gtcccgttgc gtaagcgaaa
     1861 acattttctg caattccacg aaggcgctct cccattcgct cagccgctgc atatagtcct
     1921 gttgcagttg ctctaatgcg ttcagcaaat gtctttccag ctccgtcact ctgtgtcact
     1981 ccgtcagttg tacccagtcc ttcccctgat agggaatcac cgttgccgat ttcccgtacg
     2041 gaaggaccag aaatttcctt ctcccgtcct gcacaaactc cacgccccat gtcttcgcgt
     2101 tcagtttctg caatgtctct tcctgtatcc tgatttcttc caggttcgcc tgtatccgcc
     2161 cgccaagata ccagagcgtc ccgccactcg cggtaaacag gagaaagact atccccagca
     2221 acctcatgcc cgtattccct gccagcttta acatgtcctt ccggtgctgc atcatcgcct
     2281 ctttcacccc ttcccggtgt tttttcagcg attcctccgt cgaggccgta aactgggcta
     2341 tagcgccttt gattttcgtc tcgtttgagg tcacagcctc gcttacagat tcgccgagct
     2401 tcctgaattc gtcgttcagc attttctctg tagattcggc tctctctttc agctttttct
     2461 cgaactccac gcccgtctgt aaaagattgc tcataaaatg ctcctttcag cctgatattc
     2521 ttcccgccgt tcgggtctgc aatgctgata ctgcttcgcg tcaccctgac cacttcaagc
     2581 cctgcctctg tgagcgcctg aatcacatcc tgacggcctc ttatctcccc gacatgataa
     2641 agagcgtcta tcccgcgtgt gacgctctct gcaagcgcct gtttcgtttc cggcaggtta
     2701 tcagggagtg tcagtatccg gcggttctcc ggggcgttgg ggtcatgcag cccgtaatgg
     2761 tgattcacca gcgtctgcca ggcatcaatt ctcggcctgt ctgcccggtc gtagtacggc
     2821 tggaggcgtt ttccggtctg tagctccatg ttcggaatga caaaattcag ctcaagccgt
     2881 cccttgtcct ggtgctccac ccacaggatg ctgtactgat tcttttcgag accgggcatc
     2941 agtacgcgct caaagctcgc catcactttt tcacgtcctc ccggcggcag ccccttctcc
     3001 gcgaacgaca gaacgcctga cgtgtatttc ttcgcaaatg gcgtggcatc gatgagttcc
     3061 cggacttctt ccggattacc ctgaagcacc gttgcgcctt cgcggttacg ctccctcccc
     3121 agcaggtaat caaccggacc actgccacca ccttttcccc tggcatgaaa tttaactatc
     3181 atcccgcgtc ccctgttccc tgacggccag acgcagccgg cgcagctcgt ccccgatggc
     3241 catcagtgcg gccaccacct gaacccggtc accggaagac cactgcccgc tgttcacctt
     3301 acgggctgtc tgattcaggt tatttccgat ggcggccagc tgacgcagta acggcggtgc
     3361 cagtgtcggc agtttcccgg aacgggcaac cggctcaccc agacagaccc gccgcatcca
     3421 taccgccagt tgtttaccct cacagcgttc cagtaaccgg gcatgttcat catcagtaac
     3481 ccgtatcgtg agcatcctct cgcgtttcat cggtatcatt acccccatga acagaaatcc
     3541 cccttacacg gaggcatcag tgactaaaca ggaaaaaacc gcccttaaca tggcccgctt
     3601 tatcagaagc cagacattaa cgctgctgga aaagctcaac gaactggacg cggatgaaca
     3661 ggcagacatc tgtgaatcgc ttcacgacca cgccgatgag ctttaccgca gctgcctcgc
     3721 acgtttcggg gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     3781 ttgcctgtga gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag caggttttag
     3841 cgggtgtcgg ggcgcagccc tgacccagtc acgtagcgat agcggagtgt atactggctt
     3901 aactatgcgg catcgatgcg gattgtatga aaagtgcgcc atggacggtg tgaaatgccg
     3961 cacagatgcg taaggagaaa atgcaactta cgacactctt ccgcttcctc gctcacggac
     4021 tcgctgcgct cggtcgttcg gctgcggcga gcggtgccgg ctcactaaaa ggcggaaata
     4081 cggttatcca cagaatcagg ggataaggcc ggaaagaaca tgtgagtaaa aaggcaaagg
     4141 acaaaaaaag gccgcgccgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4201 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4261 gtttcccccc ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4321 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgttggta
     4381 tctcggcccg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4441 gcccgaccgc tgcgccttat ccggtaacta tcgtctcgag tccaacccgg taagacacga
     4501 caaagcgcca ctggcagcag ccattggtaa ctgaaaagtg gatttagata cgcagaactc
     4561 ttgaagttga agccttatag cggctacact gaaaggacag catttggtat ctgtgctcca
     4621 cttaagccag ctacccggtt aagcagtccc caactgactt aaccttcgac taaaccgcct
     4681 ccccaggcgg ttttttcgtt tacagggcaa gagactacga cgatctgtaa aggatctcaa
     4741 gaagatcctt tacaggctac cactactacc acttcccata ctcactccgg taacagtgga
     4801 agatggtgaa acagccccac ctccagtatc tctgacattg ttgacttggc atgctgcaag
     4861 cagcattatt cctagtgaaa tggcacaaat aataattttt ttcatatata cctttattat
     4921 aatcctatag gtcaatgatg attgatgaac attcaataga tatagacaat agaaaagcca
     4981 ataacttatt gtatttattt atggtaatcg gagttatacc tttactctgt atactagccg
     5041 tttactatac aaatccagac aatctgttct tacacacaat agctaccagt actgaaaaca
     5101 tcccatcaat aacctccgcc tacaacccat taatgacaaa ggttatggat atttattgta
     5161 aaacagcgcc ttcccttgct ttgatattat ttatcctgac atttaaaacg agaaaactaa
     5221 taaaaaaaac aaaccgaaat gctgtactta gaagttgctt attaagtcca tttgtctgtg
     5281 ctgttttttt gtatttactc tgcttccgaa accttgagct aacgacagca ggaaggccag
     5341 ctaggctaat ggccaacaat gatgcaacac tattgctatt ttatattggc ctgtacttaa
     5401 tcattttttt tataagctat ttcacgctat tcacaccagt taccacgttt aaattattaa
     5461 aagaaaggca gtaaccctgc cttcctttta cccggtattt attaatgagc gggtttaatg
     5521 atttcgttat ttagtgcatc tgcaaacttg tcatcaatta atgcacctac aactgctgcg
     5581 agtagaatac ctacgatacc gacagcaata gcaggagccc caagagataa ggcatatgcc
     5641 cctaatgtgg ctgaaaaaac ccccagggcc acagcagaag caatgccact gagcacccag
     5701 gactccacct ccagcatcag aggtccccag ttcccggttt catagccctc gatgcttttc
     5761 tcccggacct tctcaacctt catcaccaca tcggcaaatt tgaaagcctt gctgatatta
     5821 cccagtttat ttgccatatc ctgagcatca atacttcgcc aggcgttcgc cagtgcatca
     5881 ctgtcggccc tgttaaattt aaacccagga ttagacaaaa ctttatttaa tgacgccatt
     5941 gcatcatcgt agctacggat ggttttcccc tggaaatttt tgacatcatc agcaatttct
     6001 cttgctaacg acttataagc atttccaaga taatcaccga ccttattacc tatatcagag
     6061 ataacttcag cagctttatt aagtacatca ctctgaatcc ggtcacggtt catactgaaa
     6121 gaataaaaca catcataagc agctttttta gcttgattct gtcgttgagg ctcttcatta
     6181 ttaaatccta atgcctcata tatctcttcc ggggtcttat ggtcgacttt atccataata
     6241 ccagccttag ctttttcact ccctacataa ggacgaacac ctgttctagt tatagataaa
     6301 acattaccat aatcatcaag caccactaaa tagtggaact gttcaacatc aacatcaaat
     6361 tttctaacat taggaggcaa attatcccac gcatcttcag cctgttgttt tgctttttcc
     6421 tgccgctgag attcatcttt attaaaacct aacgcctcat atatctcctc aggtgtttta
     6481 tgattaattt tttccataat acccgcctta gctttttcac tccctacgta aggacggaca
     6541 cctgttcgaa gaataccgat agctctacca ttatcattaa gcgttacacg atatttaaaa
     6601 ccatcgatac tcacatcaat ctctcgcggt aagttgtttt tatcccactt acgagcaact
     6661 tcatcattag gattttcagg aatatagccc ccctgaacat cactattccc gccagcccca
     6721 ggaactccat atacaggtgt ttttaccaga ccatttgtga tggtaatgcg cacctgccca
     6781 ttatcatcaa cataaggcgg cacatccgga ttattggttt tactccatcc ccaccccgtc
     6841 tgacttccat gttcaccacc tccattacca gagcctgaac caccacccca gtgaacccca
     6901 ctattactat tattatttcc accttgctga
//
