LOCUS       CP057519                4721 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB23-C18 plasmid pRHB23-C18_4, complete
            sequence.
ACCESSION   CP057519
VERSION     CP057519.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148218
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH12 T2-C18
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 146.056715452404x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 09:28:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,813
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,577
            CDSs (with protein)               :: 4,577
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 52 of 116
            Pseudo Genes (incomplete)         :: 46 of 116
            Pseudo Genes (internal stop)      :: 37 of 116
            Pseudo Genes (multiple problems)  :: 19 of 116
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB23-C18"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB23-C18_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            558..1514
                     /locus_tag="HVY01_24050"
     CDS             558..1514
                     /locus_tag="HVY01_24050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMJ30494.1"
                     /translation="MYLIEEALTSWLLNEYKKPVITKEEISLQIYLFYLKKEYNGERIY
                     QIRKNKAGPNEFEKNILKLLKSGVLSRVESNDKHHAYPHLIGGFYEVFFVKGNDGSPEN
                     DIVCSTYPYGYLSYLSAMDWHNITDKIPKIIHFTTCRSEQWKNKFYHDFQKDNPDIDEA
                     KQFMPRFPRNSEVNGIKIIVTQETNYVEPMKARNSPVRVSSIGKTFMDMTRRPDYCGGE
                     EHVLESFIEHGKKYAPLIIKEVDKLGRHIDKVRVGFILDRIIGVNNKKISEWKQEAGKY
                     RGSSKVLSPKSPFSPYFDEEWFISINVDMVKSYGKRN"
                     /name="hypothetical protein"
     gene            1498..2364
                     /locus_tag="HVY01_24055"
     CDS             1498..2364
                     /locus_tag="HVY01_24055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="QMJ30495.1"
                     /translation="MEKEINNWIEESGGKYRLMRQAVHIILKSISIEESLNEMMVMKGG
                     TLLGVKHGSDRYTKDIDFSTEKKLSEIDLGDFFNRFNDSMDVAEAELGYGIKCKVQSHK
                     IQPNAQGTFPTVQINIAFCDRSNASAMKRLESNQSPSVINIDFSFNEKTYDFDFLSLDG
                     DDDNDSVKTYSLTDLMAEKYRSVLQQKVRERNREQDIYDINYLLGKYEFSRQDKYKILE
                     SLLKKSEGKQLDNLLNVKGIRDVEIIERSSQRYQDLSSTVKSLPLFEDAYEKVACFYES
                     LPWDIFK"
                     /name="nucleotidyl transferase AbiEii/AbiGii toxin family
                     protein"
     gene            2361..3014
                     /locus_tag="HVY01_24060"
     CDS             2361..3014
                     /locus_tag="HVY01_24060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C family protein"
                     /protein_id="QMJ30496.1"
                     /translation="MIAFSFSDIGNRCENLDLLSTINCQNDVFGMVIMDGYSCSEWNMK
                     ILSGRLDKCVFSGDLNKDIIEPCKSVPVKAAVCFLFKNNTHLHIYNAGDCRVYNKSGVL
                     LTEDNSVAWKRLKENGIDNKKIPHLVCYCPDRNYLTSFVDFCSDEIIFNHSVFDLNDEN
                     KEVILCTDGFWEHFNDGTVIDLMKDNPESNLAKFTKNIPGNAENHTVCYVRFPS"
                     /name="protein phosphatase 2C family protein"
     gene            complement(4004..4198)
                     /locus_tag="HVY01_24065"
     CDS             complement(4004..4198)
                     /locus_tag="HVY01_24065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMJ30497.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          3849..3931
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.79e-36"
     Typing          4235..4338
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.286"
                     /coverage="100.0"
                     /evalue="4.87e-36"
     Typing          4463..4718
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.512"
                     /coverage="89.0"
                     /evalue="1.21e-116"
     Typing          complement(3278..3836)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.484"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gttcagatat gtagcaatga gtcagggatt cacgaactga aaatgtccga aaatgtgtct
       61 catgggcacc gtatgcgccc aaaccgatcg ccattcactt tcatgcatag ctatgcagtg
      121 agctgaaagc gatcctgacg catttttccg gttttcgctt ggtaaaacat ctctttttgc
      181 ggtgttcgcg gcagaatcgc gttcagcgcg ttttagcggt gcgcgtaatg cgacgttatg
      241 gtaaatgacg ctgaaaaatg atcttttacg ctcatcgttt atgagcaaca gatgtcctaa
      301 tcatggattt gaactataac cgccgctgaa ttgatcgcat gaggtgttat gaaaacggtg
      361 atataagtaa actttttctt gacttcctac ttattatcct ataaaataaa tccttaggat
      421 ttattttata ggacatcaaa aatataaaaa tggcaaatgc ttgtttgtta ttattttttt
      481 cttgacaatc aatctctaat agaatatacg tccttaggac gtatattcta ttagagacta
      541 aaccgaaaag gttgaatatg tatcttatcg aagaagcgct aacgtcttgg ttgttgaatg
      601 aatataaaaa accagtcata accaaagaag aaatctcttt gcagatttac cttttttatt
      661 taaaaaaaga atataatggt gagaggatat atcaaattag aaagaacaaa gcaggaccta
      721 atgaatttga aaaaaatata ttaaaattat taaagtcagg ggttttatca agagttgaaa
      781 gtaacgataa gcatcatgct tacccccatt taattggcgg tttctatgag gtcttttttg
      841 ttaaaggtaa cgatggttct ccggaaaatg atatagtgtg ctctacgtat ccatatggat
      901 acttatccta tttatccgct atggattggc ataacatcac agataaaata cccaagataa
      961 tccactttac gacatgtcgt tccgaacagt ggaagaataa gttctatcat gattttcaaa
     1021 aggataatcc tgatatagac gaagccaaac aattcatgcc tagatttcca agaaattctg
     1081 aggtcaatgg aataaaaatt atcgttacac aagaaacaaa ttatgttgag ccaatgaagg
     1141 ctcgtaattc ccctgtcagg gtatcatcta tcggtaagac ctttatggat atgaccaggc
     1201 ggcctgatta ctgtggtggt gaggagcatg tattggaatc atttattgag cacggtaaaa
     1261 aatatgcacc tctgattata aaggaagtcg ataagcttgg ccgtcatatt gacaaggtaa
     1321 gggttggatt cattttggat agaattattg gggttaataa taaaaaaata agcgaatgga
     1381 aacaagaggc gggtaaatac agaggtagtt cgaaagtgct atctccaaag agtccattct
     1441 caccttactt tgatgaagaa tggtttattt ctattaatgt tgatatggtt aagtcttatg
     1501 gaaaaagaaa ttaataattg gatcgaagag tctggtggga agtacaggtt gatgcggcag
     1561 gctgtacata taatacttaa atctatttct atagaggaga gtttgaatga aatgatggtt
     1621 atgaagggag ggactcttct tggtgttaag catggtagtg atagatacac aaaggatatt
     1681 gatttttcta ccgaaaaaaa actttctgaa attgacttgg gggatttttt caataggttt
     1741 aatgactcaa tggatgttgc tgaggctgaa ttgggatatg gtattaaatg taaagttcaa
     1801 tctcataaaa ttcaacctaa tgcacaagga acatttccaa ctgtacagat aaatatagcc
     1861 ttttgcgatc gttctaatgc gtctgcaatg aaaaggcttg aatctaatca atcaccttca
     1921 gtgataaata tcgattttag ctttaacgaa aaaacgtatg attttgactt tttatcatta
     1981 gatggtgatg atgacaatga tagcgtaaag acctacagct taactgattt gatggctgaa
     2041 aaatatcgat ccgttttgca acaaaaagtt agagagagaa atcgagagca agatatatat
     2101 gatatcaatt atcttttagg taaatatgag ttttctcggc aagataagta taaaatactg
     2161 gagtctcttt taaaaaagag tgaaggtaaa caattggata atctcttaaa cgttaaaggt
     2221 ataagggatg tagaaattat cgagcgttcc agtcagcgat atcaggattt atcatccacc
     2281 gttaaatcat tacctctttt tgaagatgct tatgaaaaag ttgcctgttt ctatgaaagt
     2341 cttccgtggg atatattcaa atgatagcgt tcagttttag tgatattggt aacagatgtg
     2401 aaaacttaga cttattatct acgataaatt gtcaaaatga tgtgtttgga atggttatta
     2461 tggatgggta ttcctgctct gagtggaata tgaaaattct ctctggtagg ttagataagt
     2521 gcgtgttttc tggtgatctg aataaagata ttattgaacc atgcaagtca gttcctgtta
     2581 aggctgcagt atgtttcctt tttaaaaata atacgcacct ccatatatat aacgcaggag
     2641 attgccgtgt atataacaaa agtggagtcc ttctaacaga ggataactcg gttgcctgga
     2701 aaagattaaa ggaaaatgga atcgataata aaaaaatacc tcacctcgtt tgttattgcc
     2761 cagacagaaa ttatctaaca tcatttgttg atttctgctc tgatgagata attttcaatc
     2821 attcagtttt tgatttaaat gatgaaaaca aagaggttat tttatgtact gatgggtttt
     2881 gggagcattt taatgatggt actgttattg acctcatgaa agataaccca gaatctaact
     2941 tggctaaatt tactaaaaat atcccgggta acgcagagaa tcatacggtt tgctacgtta
     3001 gattcccttc ttgatttcgt gttttttgaa gaagctattg agatcttcgt gtcatataga
     3061 taatctcttt tctgtaaaag aaaaaaaccg cctttgcggg gcggttttct cttaggttca
     3121 aagagtttgc ggctcgaaga accaaggtaa ctggcttgga ggagcgcagt caccaaaacc
     3181 tgttctttca gtttagcctt aactggcgca tgacttcaag actaactcct ctaaatcagt
     3241 taccagtggc tgctgccagt ggcgttaagg cgtgtcttac cgggttggac tcaagacgat
     3301 agttaccgga taaggcgcag cagtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3361 tggagcgaac gacctacacc gaactgagat accaacagcg tgagctatga gaaagcgcca
     3421 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3481 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3541 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3601 aaaacgccag cggcgcggcc ttttgttgtt ctttgccttt ttgctcacat gttctttccg
     3661 gccttatccc ctgattctgt ggataaccgt gctgccgttt ttgtgtaagt cagtaccgct
     3721 cgccgcagcc gaacgaccga gcgtagcgag tcagtgagcg aggaagcgga aaagcgcctg
     3781 gacgcgcatt ttctccttac gcatctgtgc ggaatttcac acccggcatg gggcactttt
     3841 catacaatct gcactgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     3901 actgggtcag ggcttcgccc cgacacccgc tgacgcgccc tggcgggctt gtctgctgcc
     3961 gaaaacgctc gcggacaagc cgtgagcgcc tccgtcagct tcgttatgtt cctgtttcac
     4021 tttcggtctc aaagcgaacc tggatgctgt tctggagttc ttccgcgagt tcgtgcagtc
     4081 gttcgcacat ggccgcctgt tcgtcggcat ccagagcatc cagtttttca agcagtgtca
     4141 ggctctggct tttgataaat cgcgccatat tcagcgcagt ttgttgctgt ttgttcacct
     4201 tccgttgttc tccgtcagta ccgggtgtcg ggtggaccct gaccaggtgg taattgtaag
     4261 acgttgcggg cgacggtatt acaattgcac atcctgtcct gtcctgtttt tttaggacgt
     4321 atcatggcac cgcatatcaa ctgataaata atccctgtgc agtgcagccg ctgttgcata
     4381 aaatttcgct ctgcgtcgag tgtttcttaa tcaataattc cgctgatact ccggaaaaac
     4441 gcgagtctgt tctcatgcct gaaatgccca caccccacgc aaaaacaatt ttttgctgat
     4501 ttttctttat aaatagagag ttatgacaaa ttagttcttc ttgctctctt tgtgatattt
     4561 aaaaaagcgg tgtcggcgcg gtgttgtagc tgcgccaaca ccgcttttta ggggtggtac
     4621 tgactatttt cataaaaaac atcattttat attaggggtg ctgctagcgg cgcggtgtgt
     4681 ttttttacag gacgcccctg ggggcgctgc taggggtgtc t
//
