LOCUS       CP057518                6930 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB23-C18 plasmid pRHB23-C18_3, complete
            sequence.
ACCESSION   CP057518
VERSION     CP057518.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148218
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH12 T2-C18
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 146.056715452404x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 09:28:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,813
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,577
            CDSs (with protein)               :: 4,577
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 52 of 116
            Pseudo Genes (incomplete)         :: 46 of 116
            Pseudo Genes (internal stop)      :: 37 of 116
            Pseudo Genes (multiple problems)  :: 19 of 116
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB23-C18"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB23-C18_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            19..537
                     /locus_tag="HVY01_23995"
     CDS             19..537
                     /locus_tag="HVY01_23995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QMJ30484.1"
                     /translation="MSNLLQTGVEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIKGAIAQFTASTEESLKKHREGVKEAMMQHRKDMLKLAGNTGMRLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVLPYGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            544..777
                     /gene="mbeD"
                     /locus_tag="HVY01_24000"
     CDS             544..777
                     /gene="mbeD"
                     /locus_tag="HVY01_24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMJ30485.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="mbeD"
     gene            complement(1097..1519)
                     /gene="exc2"
                     /locus_tag="HVY01_24005"
     CDS             complement(1097..1519)
                     /gene="exc2"
                     /locus_tag="HVY01_24005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMJ30486.1"
                     /translation="MRSAAVRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFHTQKADTIRMMHPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEEFLQSLRTTTT
                     FAGRKYTNSDMPSPEKMKLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            1641..1919
                     /locus_tag="HVY01_24010"
     CDS             1641..1919
                     /locus_tag="HVY01_24010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMJ30487.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLASLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(1911..2201)
                     /locus_tag="HVY01_24015"
     CDS             complement(1911..2201)
                     /locus_tag="HVY01_24015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMJ30488.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            2442..3941
                     /gene="csa"
                     /locus_tag="HVY01_24020"
     CDS             2442..3941
                     /gene="csa"
                     /locus_tag="HVY01_24020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /protein_id="QMJ30489.1"
                     /translation="MAKELSGYGPTAGESVGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGGEHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPGAGGNSDVQG
                     GYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEK
                     AKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDNLPPNVRKFDVDVEQF
                     HYLVVLDDYGNVLSITRTGVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEEPQRQ
                     NQAKKAAYDVFYSFSMNRDRIQSDVLNKAAEVISDIGNKVGDYLGNAYKSLAREIADDV
                     KNFQGKTIRSYDDAMASLNKVLSNPGFKFNRADSDALANAWRSIDAQDMANKLGNISKA
                     FKFADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASAVALGVFSATLGAYAL
                     SLGAPAIAVGIVGILLAAVVGALIDDKFADALNNEIIKPAH"
                     /name="csa"
     gene            complement(3969..4508)
                     /gene="csi"
                     /locus_tag="HVY01_24025"
     CDS             complement(3969..4508)
                     /gene="csi"
                     /locus_tag="HVY01_24025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 immunity protein"
                     /protein_id="QMJ30490.1"
                     /translation="MMIDEHSIDIDNRKANNLLYLFMVIGVIPLLCILAVYYTNPDNLF
                     LHTIATSTENIPSITSAYNPLMTKVMDIYCKTAPSLALILFILTFKTRKLIKKTNRNAV
                     LRSCLLSPFVCAVFLYLLCFRNLELTTAGRPARLMANNDATLLLFYIGLYLIIFFISYF
                     TLFTPVTTFKLLKERQ"
                     /name="csi"
     gene            4538..4693
                     /gene="csl"
                     /locus_tag="HVY01_24030"
     CDS             4538..4693
                     /gene="csl"
                     /locus_tag="HVY01_24030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="QMJ30491.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GSSGSL"
                     /name="csl"
     gene            complement(5692..5883)
                     /locus_tag="HVY01_24035"
     CDS             complement(5692..5883)
                     /locus_tag="HVY01_24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMJ30492.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5948..6271
                     /locus_tag="HVY01_24040"
     CDS             5948..6271
                     /locus_tag="HVY01_24040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMJ30493.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            6261..6929
                     /locus_tag="HVY01_24045"
                     /pseudo=""
     CDS             6261..6929
                     /locus_tag="HVY01_24045"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          5520..5607
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          complement(4951..5497)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.171"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4..881
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.0"
                     /coverage="99.0"
                     /evalue="5.27e-141"
     Typing          6262..6929
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.103"
                     /coverage="99.0"
                     /evalue="3.58e-142"
ORIGIN
        1 ggctgaaagg agcattttat gagcaatctt ttacagacgg gcgtggagtt cgagaaaaag
       61 ctgaaagaga gagccgaatc tacagagaaa atgctgaacg acgaattcag gaagctcggc
      121 gaatctgtaa gcgaggctgt gacctcaaac gagacgaaaa tcaaaggcgc tatagcccag
      181 tttacggcct cgacggagga atcgctgaaa aaacaccggg aaggggtgaa agaggcgatg
      241 atgcagcacc ggaaggacat gttaaagctg gcagggaata cgggcatgag gttgctgggg
      301 atagtctttc tcctgtttac cgcgagtggc gggacgctct ggtatcttgg cgggcggata
      361 caggcgaacc tggaagaaat caggatacag gaagagacat tgcagaaact gaacgcgaag
      421 acatggggcg tggagtttgt gcaggacggg agaaggaaat ttctggtcct tccgtacggg
      481 aaatcggcaa cggtgattcc ctatcagggg aaggactggg tacaactgac ggagtgacac
      541 agagtgacgg agctggaaag acatttgctg aacgcattag agcaactgca acaggactat
      601 atgcagcggc tgagcgaatg ggagagcgcc ttcgtggaat tgcagaaaat gttttcgctt
      661 acgcaacggg acaacgcgat gctgaacgag caggtcatgc agttgagtca gcaggtgcag
      721 cacttgagcg agcggacaga acgcttgagc cagttataca gcgagaacag gagataaggg
      781 acgaacggct gatacaggaa cgcgaacatg cgttatccct ggaacgggag cgtcagccgg
      841 aaatacagga acgcacgctg gatggtcctt cgctgggatg gtgaaaccat gaaaaatggc
      901 agcttcagtg gattaagagg ggttaatgtg gcctgtacca tctggttgca taggtattca
      961 tgcggttaaa atttatcagg cgcgatcgcg gcagtttttc gggtggtttg ttgccatttt
     1021 tacctgtctg ctgccgtgat cgcgctgaac gcgtttcagc ggtgcgtaca ataagggatt
     1081 atggtaaatg tctcccttac tgcctgccct cgtagccatc gagataaacc gcagaaatcg
     1141 tgtcagccag cagcttcatt ttttccggtg atggcatatc actgttcgtg tattttctgc
     1201 ccgcaaaggt ggttgtggtt ctcagtgact gaaggaattc ttcagagtgg aattctttta
     1261 cacgttgctg tgccgtttct gctgatactc cggctgcttt gtctttcatc cccatatccc
     1321 ggaactgctg aaagaacggg tgcatcatac gtatggtgtc tgctttctgg gtgtggaagt
     1381 taggatcaaa tccatcatct gaagcataaa cataatgccg ggtatgtcgt tccaggctgg
     1441 tctttggtgt gcatcctgta agagctattg ttgctgttat cagaggtatc agaagcagcc
     1501 tgacagcggc agatctcata aacatctcct tgcgttttta atcttgatac tcagggagtt
     1561 atatctccga agagtatctc ttatcatacg tcggtttact cccaatgtga cttgatggtc
     1621 aatgagtcag gaataatccc gtgaaaaaat gtgtgccagt cctgcttttt agtgttcttt
     1681 ctgcttcggt tgccagtcat gcggacagca atgtttttca acaaaaggcc gagaagacaa
     1741 aaaacggcat tgttgcggat gttaatgcca gacagaaaat gttcagtaac agactgaaga
     1801 tacaacttca gatcgatgag ttgagagcag agctggctgg caccagagat gcccaaaaaa
     1861 gagaggattt gcgtaaaaaa ctggcgtctc tggagcggca aaagaaatcc ttacgctgat
     1921 gggggcgtat tttccccggt ttcttccata acctttttac cgttcagata tttacgggcg
     1981 aggcgaatgc gtcttgcctg gtataccgcc gagtaagcct gggtgggatt cacaccgtta
     2041 ctgatttcac tgaccacatg acgctgatca gccagtgtca gcgtgtaaac ctgtttctgg
     2101 atgaggtcac gggctttgtc atgctcatat ccggcagcaa ccagggtgtc gagaagtcgc
     2161 ttgtctgttt tgttcagttt atggcgggga gatttgctca catcgttctc cgttttttca
     2221 tgatccaaca gttttatatc tcatcctgat ttgctccggt gttctgtttc gacgttctca
     2281 atgtctgcct ccccatagtg tgatccttat tgcaaaaatg aaaatccatg ctcttgacat
     2341 ggataatgct gagtagtagg tttttgctga atacaaaacc agtggttata tgtacagtat
     2401 attgctttta atttattgtt ttaattatca aagaggaatt tatggctaaa gaactaagtg
     2461 gatatggccc aactgctggt gagtctgtgg ggggaacagg ggcgaatctg aatcagcaag
     2521 gtggaaataa taatagtaat agtggggttc actggggtgg tggttcaggc tctggtaatg
     2581 gaggtggtga acatggaagt cagacggggt ggggatggag taaaaccaat aatccggatg
     2641 tgccgcctta tgttgatgat aatgggcagg tgcgcattac catcacaaat ggtctggtaa
     2701 aaacacctgt atatggagtt cctggggctg gcgggaatag tgatgttcag gggggctata
     2761 ttcctgaaaa tcctaatgat gaagttgctc gtaagtggga taaaaacaac ttaccgcgag
     2821 agattgatgt gagtatcgat ggttttaaat atcgtgtaac gcttaatgat aatggtagag
     2881 ctatcggtat tcttcgaaca ggtgtccgtc cttacgtagg gagtgaaaaa gctaaggcgg
     2941 gtattatgga aaaaattaat cataaaacac ctgaggagat atatgaggcg ttaggtttta
     3001 ataaagatga atctcagcgg caggaaaaag caaaacaaca ggctgaagat gcgtgggata
     3061 atttgcctcc taatgttaga aaatttgatg ttgatgttga acagttccac tatttagtgg
     3121 tgcttgatga ttatggtaat gttttatcta taactagaac aggtgttcgt ccttatgtag
     3181 ggagtgaaaa agctaaggct ggtattatgg ataaagtcga ccataagacc ccggaagaga
     3241 tatatgaggc attaggattt aataatgaag agcctcaacg acagaatcaa gctaaaaaag
     3301 ctgcttatga tgtgttttat tctttcagta tgaaccgtga ccggattcag agtgatgtac
     3361 ttaataaagc tgctgaagtt atctctgata taggtaataa ggtcggtgat tatcttggaa
     3421 atgcttataa gtcgttagca agagaaattg ctgatgatgt caaaaatttc caggggaaaa
     3481 ccatccgtag ctacgatgat gcaatggcgt cattaaataa agttttgtct aatcctgggt
     3541 ttaaatttaa cagggccgac agtgatgcac tggcgaacgc ctggcgaagt attgatgctc
     3601 aggatatggc aaataaactg ggtaatatca gcaaggcttt caaatttgcc gatgtggtga
     3661 tgaaggttga gaaggtccgg gagaaaagca tcgagggcta tgaaaccggg aactggggac
     3721 ctctgatgct ggaggtggag tcctgggtgc tcagtggcat tgcttctgct gtggccctgg
     3781 gggttttttc agccacatta ggggcatatg ccttatctct tggggctcct gctattgctg
     3841 tcggtatcgt aggtattcta ctcgcagcag ttgtaggtgc attaattgat gacaagtttg
     3901 cagatgcact aaataacgaa atcattaaac ccgctcatta ataaataccg ggtaaaagga
     3961 aggcagggtt actgcctttc ttttaataat ttaaacgtgg taactggtgt gaatagcgtg
     4021 aaatagctta taaaaaaaat gattaagtac aggccaatat aaaatagcaa tagtgttgca
     4081 tcattgttgg ccattagcct agctggcctt cctgctgtcg ttagctcaag gtttcggaag
     4141 cagagtaaat acaaaaaaac agcacagaca aatggactta ataagcaact tctaagtaca
     4201 gcatttcggt ttgttttttt tattagtttt ctcgttttaa atgtcaggat aaataatatc
     4261 aaagcaaggg aaggcgctgt tttacaataa atatccataa cctttgtcat taatgggttg
     4321 taggcggagg ttattgatgg gatgttttca gtactggtag ctattgtgtg taagaacaga
     4381 ttgtctggat ttgtatagta aacggctagt atacagagta aaggtataac tccgattacc
     4441 ataaataaat acaataagtt attggctttt ctattgtcta tatctattga atgttcatca
     4501 atcatcattg acctatagga ttataataaa ggtatatatg aaaaaaatta ttatttgtgc
     4561 catttcacta ggaataatgc tgcttgcagc atgccaagtc aacaatgtca gagatactgg
     4621 aggtggggct gtttcaccat cttccactgt taccggagtg agtatgggaa gtggtagtag
     4681 tggtagcctg taaaggatct tcttgagatc ctttacagat cgtcgtagtc tcttgccctg
     4741 taaacgaaaa aaccgcctgg ggaggcggtt tagtcgaagg ttaagtcagt tggggactgc
     4801 ttaaccgggt agctggctta agtggagcac agataccaaa tgctgtcctt tcagtgtagc
     4861 cgctataagg cttcaacttc aagagttctg cgtatctaaa tccacttttc agttaccaat
     4921 ggctgctgcc agtggcgctt tgtcgtgtct taccgggttg gactcgagac gatagttacc
     4981 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     5041 aacgacctac accgggccga gataccaaca gcgtgagcta tgagaaagcg ccacgcttcc
     5101 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     5161 gagggagctt ccggggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     5221 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     5281 cagcggcgcg gccttttttt gtcctttgcc tttttactca catgttcttt ccggccttat
     5341 cccctgattc tgtggataac cgtatttccg ccttttagtg agccggcacc gctcgccgca
     5401 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagtgt cgtaagttgc
     5461 attttctcct tacgcatctg tgcggcattt cacaccgtcc atggcgcact tttcatacaa
     5521 tccgcatcga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt
     5581 cagggctgcg ccccgacacc cgctaaaacc tgctgacgcg ccctgacggg cttgtctgct
     5641 cccggcatcc gctcacaggc aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     5701 ttcaccgtca tccccgaaac gtgcgaggca gctgcggtaa agctcatcgg cgtggtcgtg
     5761 aagcgattca cagatgtctg cctgttcatc cgcgtccagt tcgttgagct tttccagcag
     5821 cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttagt
     5881 cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg
     5941 cgagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgctg
     6001 tgagggtaaa caactggcgg tatggatgcg gcgggtctgt ctgggtgagc cggttgcccg
     6061 ttccgggaaa ctgccgacac tggcaccgcc gttactgcgt cagctggccg ccatcggaaa
     6121 taacctgaat cagacagccc gtaaggtgaa cagcgggcag tggtcttccg gtgaccgggt
     6181 tcaggtggtg gccgcactga tggccatcgg ggacgagctg cgccggctgc gtctggccgt
     6241 cagggaacag gggacgcggg atgatagtta aatttcatgc caggggaaaa ggtggtggca
     6301 gtggtccggt tgattacctg ctggggaggg agcgtaaccg cgaaggcgca acggtgcttc
     6361 agggtaatcc ggaagaagtc cgggaactca tcgatgccac gccatttgcg aagaaataca
     6421 cgtcaggcgt tctgtcgttc gcggagaagg agctgccgcc gggaggacgt gaaaaagtga
     6481 tggcgagctt tgagcgcgta ctgatgcccg gtctcgaaaa gaatcagtac agcatcctgt
     6541 gggtggagca ccaggacaag ggacggcttg agctgaattt tgtcattccg aacatggagc
     6601 tacagaccgg aaaacgcctc cagccgtact acgaccgggc agacaggccg agaattgatg
     6661 cctggcagac gctggtgaat caccattacg ggctgcatga ccccaacgcc ccggagaacc
     6721 gccggatact gacactccct gataacctgc cggaaacgaa acaggcgctt gcagagagcg
     6781 tcacacgcgg gatagacgct ctttatcatg tcggggagat aagaggccgt caggatgtga
     6841 ttcaggcgct cacagaggca gggcttgaag tggtcagggt gacgcgaagc agtatcagca
     6901 ttgcagaccc gaacggcggg aagaatatca
//
