LOCUS       CP057515                4721 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB23-C24 plasmid pRHB23-C24_4, complete
            sequence.
ACCESSION   CP057515
VERSION     CP057515.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148219
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH12 T2-C24
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 151.508294823458x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 09:28:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,813
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,576
            CDSs (with protein)               :: 4,576
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 53 of 117
            Pseudo Genes (incomplete)         :: 46 of 117
            Pseudo Genes (internal stop)      :: 37 of 117
            Pseudo Genes (multiple problems)  :: 19 of 117
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB23-C24"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB23-C24_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(4311..4721,1..243)
                     /locus_tag="HVY02_24050"
     CDS             join(4311..4721,1..243)
                     /locus_tag="HVY02_24050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C family protein"
                     /protein_id="QMJ25650.1"
                     /translation="MIAFSFSDIGNRCENLDLLSTINCQNDVFGMVIMDGYSCSEWNMK
                     ILSGRLDKCVFSGDLNKDIIEPCKSVPVKAAVCFLFKNNTHLHIYNAGDCRVYNKSGVL
                     LTEDNSVAWKRLKENGIDNKKIPHLVCYCPDRNYLTSFVDFCSDEIIFNHSVFDLNDEN
                     KEVILCTDGFWEHFNDGTVIDLMKDNPESNLAKFTKNIPGNAENHTVCYVRFPS"
                     /name="protein phosphatase 2C family protein"
     gene            complement(1233..1427)
                     /locus_tag="HVY02_24055"
     CDS             complement(1233..1427)
                     /locus_tag="HVY02_24055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMJ25651.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2508..3464
                     /locus_tag="HVY02_24060"
     CDS             2508..3464
                     /locus_tag="HVY02_24060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMJ25652.1"
                     /translation="MYLIEEALTSWLLNEYKKPVITKEEISLQIYLFYLKKEYNGERIY
                     QIRKNKAGPNEFEKNILKLLKSGVLSRVESNDKHHAYPHLIGGFYEVFFVKGNDGSPEN
                     DIVCSTYPYGYLSYLSAMDWHNITDKIPKIIHFTTCRSEQWKNKFYHDFQKDNPDIDEA
                     KQFMPRFPRNSEVNGIKIIVTQETNYVEPMKARNSPVRVSSIGKTFMDMTRRPDYCGGE
                     EHVLESFIEHGKKYAPLIIKEVDKLGRHIDKVRVGFILDRIIGVNNKKISEWKQEAGKY
                     RGSSKVLSPKSPFSPYFDEEWFISINVDMVKSYGKRN"
                     /name="hypothetical protein"
     gene            3448..4314
                     /locus_tag="HVY02_24065"
     CDS             3448..4314
                     /locus_tag="HVY02_24065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="QMJ25653.1"
                     /translation="MEKEINNWIEESGGKYRLMRQAVHIILKSISIEESLNEMMVMKGG
                     TLLGVKHGSDRYTKDIDFSTEKKLSEIDLGDFFNRFNDSMDVAEAELGYGIKCKVQSHK
                     IQPNAQGTFPTVQINIAFCDRSNASAMKRLESNQSPSVINIDFSFNEKTYDFDFLSLDG
                     DDDNDSVKTYSLTDLMAEKYRSVLQQKVRERNREQDIYDINYLLGKYEFSRQDKYKILE
                     SLLKKSEGKQLDNLLNVKGIRDVEIIERSSQRYQDLSSTVKSLPLFEDAYEKVACFYES
                     LPWDIFK"
                     /name="nucleotidyl transferase AbiEii/AbiGii toxin family
                     protein"
     Typing          1078..1160
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.79e-36"
     Typing          1464..1567
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.286"
                     /coverage="100.0"
                     /evalue="4.87e-36"
     Typing          1692..1947
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.512"
                     /coverage="89.0"
                     /evalue="1.21e-116"
     Typing          complement(507..1065)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.484"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tatctaacat catttgttga tttctgctct gatgagataa ttttcaatca ttcagttttt
       61 gatttaaatg atgaaaacaa agaggttatt ttatgtactg atgggttttg ggagcatttt
      121 aatgatggta ctgttattga cctcatgaaa gataacccag aatctaactt ggctaaattt
      181 actaaaaata tcccgggtaa cgcagagaat catacggttt gctacgttag attcccttct
      241 tgatttcgtg ttttttgaag aagctattga gatcttcgtg tcatatagat aatctctttt
      301 ctgtaaaaga aaaaaaccgc ctttgcgggg cggttttctc ttaggttcaa agagtttgcg
      361 gctcgaagaa ccaaggtaac tggcttggag gagcgcagtc accaaaacct gttctttcag
      421 tttagcctta actggcgcat gacttcaaga ctaactcctc taaatcagtt accagtggct
      481 gctgccagtg gcgttaaggc gtgtcttacc gggttggact caagacgata gttaccggat
      541 aaggcgcagc agtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
      601 acctacaccg aactgagata ccaacagcgt gagctatgag aaagcgccac gcttcccgaa
      661 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      721 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      781 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
      841 ggcgcggcct tttgttgttc tttgcctttt tgctcacatg ttctttccgg ccttatcccc
      901 tgattctgtg gataaccgtg ctgccgtttt tgtgtaagtc agtaccgctc gccgcagccg
      961 aacgaccgag cgtagcgagt cagtgagcga ggaagcggaa aagcgcctgg acgcgcattt
     1021 tctccttacg catctgtgcg gaatttcaca cccggcatgg ggcacttttc atacaatctg
     1081 cactgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcagg
     1141 gcttcgcccc gacacccgct gacgcgccct ggcgggcttg tctgctgccg aaaacgctcg
     1201 cggacaagcc gtgagcgcct ccgtcagctt cgttatgttc ctgtttcact ttcggtctca
     1261 aagcgaacct ggatgctgtt ctggagttct tccgcgagtt cgtgcagtcg ttcgcacatg
     1321 gccgcctgtt cgtcggcatc cagagcatcc agtttttcaa gcagtgtcag gctctggctt
     1381 ttgataaatc gcgccatatt cagcgcagtt tgttgctgtt tgttcacctt ccgttgttct
     1441 ccgtcagtac cgggtgtcgg gtggaccctg accaggtggt aattgtaaga cgttgcgggc
     1501 gacggtatta caattgcaca tcctgtcctg tcctgttttt ttaggacgta tcatggcacc
     1561 gcatatcaac tgataaataa tccctgtgca gtgcagccgc tgttgcataa aatttcgctc
     1621 tgcgtcgagt gtttcttaat caataattcc gctgatactc cggaaaaacg cgagtctgtt
     1681 ctcatgcctg aaatgcccac accccacgca aaaacaattt tttgctgatt tttctttata
     1741 aatagagagt tatgacaaat tagttcttct tgctctcttt gtgatattta aaaaagcggt
     1801 gtcggcgcgg tgttgtagct gcgccaacac cgctttttag gggtggtact gactattttc
     1861 ataaaaaaca tcattttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
     1921 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
     1981 cacgaactga aaatgtccga aaatgtgtct catgggcacc gtatgcgccc aaaccgatcg
     2041 ccattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
     2101 gttttcgctt ggtaaaacat ctctttttgc ggtgttcgcg gcagaatcgc gttcagcgcg
     2161 ttttagcggt gcgcgtaatg cgacgttatg gtaaatgacg ctgaaaaatg atcttttacg
     2221 ctcatcgttt atgagcaaca gatgtcctaa tcatggattt gaactataac cgccgctgaa
     2281 ttgatcgcat gaggtgttat gaaaacggtg atataagtaa actttttctt gacttcctac
     2341 ttattatcct ataaaataaa tccttaggat ttattttata ggacatcaaa aatataaaaa
     2401 tggcaaatgc ttgtttgtta ttattttttt cttgacaatc aatctctaat agaatatacg
     2461 tccttaggac gtatattcta ttagagacta aaccgaaaag gttgaatatg tatcttatcg
     2521 aagaagcgct aacgtcttgg ttgttgaatg aatataaaaa accagtcata accaaagaag
     2581 aaatctcttt gcagatttac cttttttatt taaaaaaaga atataatggt gagaggatat
     2641 atcaaattag aaagaacaaa gcaggaccta atgaatttga aaaaaatata ttaaaattat
     2701 taaagtcagg ggttttatca agagttgaaa gtaacgataa gcatcatgct tacccccatt
     2761 taattggcgg tttctatgag gtcttttttg ttaaaggtaa cgatggttct ccggaaaatg
     2821 atatagtgtg ctctacgtat ccatatggat acttatccta tttatccgct atggattggc
     2881 ataacatcac agataaaata cccaagataa tccactttac gacatgtcgt tccgaacagt
     2941 ggaagaataa gttctatcat gattttcaaa aggataatcc tgatatagac gaagccaaac
     3001 aattcatgcc tagatttcca agaaattctg aggtcaatgg aataaaaatt atcgttacac
     3061 aagaaacaaa ttatgttgag ccaatgaagg ctcgtaattc ccctgtcagg gtatcatcta
     3121 tcggtaagac ctttatggat atgaccaggc ggcctgatta ctgtggtggt gaggagcatg
     3181 tattggaatc atttattgag cacggtaaaa aatatgcacc tctgattata aaggaagtcg
     3241 ataagcttgg ccgtcatatt gacaaggtaa gggttggatt cattttggat agaattattg
     3301 gggttaataa taaaaaaata agcgaatgga aacaagaggc gggtaaatac agaggtagtt
     3361 cgaaagtgct atctccaaag agtccattct caccttactt tgatgaagaa tggtttattt
     3421 ctattaatgt tgatatggtt aagtcttatg gaaaaagaaa ttaataattg gatcgaagag
     3481 tctggtggga agtacaggtt gatgcggcag gctgtacata taatacttaa atctatttct
     3541 atagaggaga gtttgaatga aatgatggtt atgaagggag ggactcttct tggtgttaag
     3601 catggtagtg atagatacac aaaggatatt gatttttcta ccgaaaaaaa actttctgaa
     3661 attgacttgg gggatttttt caataggttt aatgactcaa tggatgttgc tgaggctgaa
     3721 ttgggatatg gtattaaatg taaagttcaa tctcataaaa ttcaacctaa tgcacaagga
     3781 acatttccaa ctgtacagat aaatatagcc ttttgcgatc gttctaatgc gtctgcaatg
     3841 aaaaggcttg aatctaatca atcaccttca gtgataaata tcgattttag ctttaacgaa
     3901 aaaacgtatg attttgactt tttatcatta gatggtgatg atgacaatga tagcgtaaag
     3961 acctacagct taactgattt gatggctgaa aaatatcgat ccgttttgca acaaaaagtt
     4021 agagagagaa atcgagagca agatatatat gatatcaatt atcttttagg taaatatgag
     4081 ttttctcggc aagataagta taaaatactg gagtctcttt taaaaaagag tgaaggtaaa
     4141 caattggata atctcttaaa cgttaaaggt ataagggatg tagaaattat cgagcgttcc
     4201 agtcagcgat atcaggattt atcatccacc gttaaatcat tacctctttt tgaagatgct
     4261 tatgaaaaag ttgcctgttt ctatgaaagt cttccgtggg atatattcaa atgatagcgt
     4321 tcagttttag tgatattggt aacagatgtg aaaacttaga cttattatct acgataaatt
     4381 gtcaaaatga tgtgtttgga atggttatta tggatgggta ttcctgctct gagtggaata
     4441 tgaaaattct ctctggtagg ttagataagt gcgtgttttc tggtgatctg aataaagata
     4501 ttattgaacc atgcaagtca gttcctgtta aggctgcagt atgtttcctt tttaaaaata
     4561 atacgcacct ccatatatat aacgcaggag attgccgtgt atataacaaa agtggagtcc
     4621 ttctaacaga ggataactcg gttgcctgga aaagattaaa ggaaaatgga atcgataata
     4681 aaaaaatacc tcacctcgtt tgttattgcc cagacagaaa t
//
