LOCUS       CP057514                6930 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB23-C24 plasmid pRHB23-C24_3, complete
            sequence.
ACCESSION   CP057514
VERSION     CP057514.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148219
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH12 T2-C24
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 151.508294823458x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 09:28:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,813
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,576
            CDSs (with protein)               :: 4,576
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 53 of 117
            Pseudo Genes (incomplete)         :: 46 of 117
            Pseudo Genes (internal stop)      :: 37 of 117
            Pseudo Genes (multiple problems)  :: 19 of 117
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB23-C24"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB23-C24_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(6834..6930,1..443))
                     /gene="csi"
                     /locus_tag="HVY02_23995"
     CDS             complement(join(6834..6930,1..443))
                     /gene="csi"
                     /locus_tag="HVY02_23995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 immunity protein"
                     /protein_id="QMJ25648.1"
                     /translation="MMIDEHSIDIDNRKANNLLYLFMVIGVIPLLCILAVYYTNPDNLF
                     LHTIATSTENIPSITSAYNPLMTKVMDIYCKTAPSLALILFILTFKTRKLIKKTNRNAV
                     LRSCLLSPFVCAVFLYLLCFRNLELTTAGRPARLMANNDATLLLFYIGLYLIIFFISYF
                     TLFTPVTTFKLLKERQ"
                     /name="csi"
     gene            473..628
                     /gene="csl"
                     /locus_tag="HVY02_24000"
     CDS             473..628
                     /gene="csl"
                     /locus_tag="HVY02_24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="QMJ25640.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GSSGSL"
                     /name="csl"
     gene            complement(1627..1818)
                     /locus_tag="HVY02_24005"
     CDS             complement(1627..1818)
                     /locus_tag="HVY02_24005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMJ25641.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1883..2206
                     /locus_tag="HVY02_24010"
     CDS             1883..2206
                     /locus_tag="HVY02_24010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMJ25649.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2196..2906
                     /locus_tag="HVY02_24015"
                     /pseudo=""
     CDS             2196..2906
                     /locus_tag="HVY02_24015"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2884..3402
                     /locus_tag="HVY02_24020"
     CDS             2884..3402
                     /locus_tag="HVY02_24020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QMJ25642.1"
                     /translation="MSNLLQTGVEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIKGAIAQFTASTEESLKKHREGVKEAMMQHRKDMLKLAGNTGMRLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVLPYGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            3409..3642
                     /gene="mbeD"
                     /locus_tag="HVY02_24025"
     CDS             3409..3642
                     /gene="mbeD"
                     /locus_tag="HVY02_24025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMJ25643.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="mbeD"
     gene            complement(3962..4384)
                     /gene="exc2"
                     /locus_tag="HVY02_24030"
     CDS             complement(3962..4384)
                     /gene="exc2"
                     /locus_tag="HVY02_24030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMJ25644.1"
                     /translation="MRSAAVRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFHTQKADTIRMMHPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEEFLQSLRTTTT
                     FAGRKYTNSDMPSPEKMKLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            4506..4784
                     /locus_tag="HVY02_24035"
     CDS             4506..4784
                     /locus_tag="HVY02_24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMJ25645.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLASLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(4776..5066)
                     /locus_tag="HVY02_24040"
     CDS             complement(4776..5066)
                     /locus_tag="HVY02_24040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMJ25646.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            5307..6806
                     /gene="csa"
                     /locus_tag="HVY02_24045"
     CDS             5307..6806
                     /gene="csa"
                     /locus_tag="HVY02_24045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /protein_id="QMJ25647.1"
                     /translation="MAKELSGYGPTAGESVGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGGEHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPGAGGNSDVQG
                     GYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEK
                     AKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDNLPPNVRKFDVDVEQF
                     HYLVVLDDYGNVLSITRTGVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEEPQRQ
                     NQAKKAAYDVFYSFSMNRDRIQSDVLNKAAEVISDIGNKVGDYLGNAYKSLAREIADDV
                     KNFQGKTIRSYDDAMASLNKVLSNPGFKFNRADSDALANAWRSIDAQDMANKLGNISKA
                     FKFADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASAVALGVFSATLGAYAL
                     SLGAPAIAVGIVGILLAAVVGALIDDKFADALNNEIIKPAH"
                     /name="csa"
     Typing          1455..1542
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          complement(886..1432)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.171"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2197..3746
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.603"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agcaatagtg ttgcatcatt gttggccatt agcctagctg gccttcctgc tgtcgttagc
       61 tcaaggtttc ggaagcagag taaatacaaa aaaacagcac agacaaatgg acttaataag
      121 caacttctaa gtacagcatt tcggtttgtt ttttttatta gttttctcgt tttaaatgtc
      181 aggataaata atatcaaagc aagggaaggc gctgttttac aataaatatc cataaccttt
      241 gtcattaatg ggttgtaggc ggaggttatt gatgggatgt tttcagtact ggtagctatt
      301 gtgtgtaaga acagattgtc tggatttgta tagtaaacgg ctagtataca gagtaaaggt
      361 ataactccga ttaccataaa taaatacaat aagttattgg cttttctatt gtctatatct
      421 attgaatgtt catcaatcat cattgaccta taggattata ataaaggtat atatgaaaaa
      481 aattattatt tgtgccattt cactaggaat aatgctgctt gcagcatgcc aagtcaacaa
      541 tgtcagagat actggaggtg gggctgtttc accatcttcc actgttaccg gagtgagtat
      601 gggaagtggt agtagtggta gcctgtaaag gatcttcttg agatccttta cagatcgtcg
      661 tagtctcttg ccctgtaaac gaaaaaaccg cctggggagg cggtttagtc gaaggttaag
      721 tcagttgggg actgcttaac cgggtagctg gcttaagtgg agcacagata ccaaatgctg
      781 tcctttcagt gtagccgcta taaggcttca acttcaagag ttctgcgtat ctaaatccac
      841 ttttcagtta ccaatggctg ctgccagtgg cgctttgtcg tgtcttaccg ggttggactc
      901 gagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
      961 gcccagcttg gagcgaacga cctacaccgg gccgagatac caacagcgtg agctatgaga
     1021 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1081 aacaggagag cgcacgaggg agcttccggg gggaaacgcc tggtatcttt atagtcctgt
     1141 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1201 cctatggaaa aacgccagcg gcgcggcctt tttttgtcct ttgccttttt actcacatgt
     1261 tctttccggc cttatcccct gattctgtgg ataaccgtat ttccgccttt tagtgagccg
     1321 gcaccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     1381 agtgtcgtaa gttgcatttt ctccttacgc atctgtgcgg catttcacac cgtccatggc
     1441 gcacttttca tacaatccgc atcgatgccg catagttaag ccagtataca ctccgctatc
     1501 gctacgtgac tgggtcaggg ctgcgccccg acacccgcta aaacctgctg acgcgccctg
     1561 acgggcttgt ctgctcccgg catccgctca caggcaagct gtgaccgtct ccgggagctg
     1621 catgtgtcag aggttttcac cgtcatcccc gaaacgtgcg aggcagctgc ggtaaagctc
     1681 atcggcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagttcgtt
     1741 gagcttttcc agcagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
     1801 tttttcctgt ttagtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat
     1861 gataccgatg aaacgcgaga ggatgctcac gatacgggtt actgatgatg aacatgcccg
     1921 gttactggaa cgctgtgagg gtaaacaact ggcggtatgg atgcggcggg tctgtctggg
     1981 tgagccggtt gcccgttccg ggaaactgcc gacactggca ccgccgttac tgcgtcagct
     2041 ggccgccatc ggaaataacc tgaatcagac agcccgtaag gtgaacagcg ggcagtggtc
     2101 ttccggtgac cgggttcagg tggtggccgc actgatggcc atcggggacg agctgcgccg
     2161 gctgcgtctg gccgtcaggg aacaggggac gcgggatgat agttaaattt catgccaggg
     2221 gaaaaggtgg tggcagtggt ccggttgatt acctgctggg gagggagcgt aaccgcgaag
     2281 gcgcaacggt gcttcagggt aatccggaag aagtccggga actcatcgat gccacgccat
     2341 ttgcgaagaa atacacgtca ggcgttctgt cgttcgcgga gaaggagctg ccgccgggag
     2401 gacgtgaaaa agtgatggcg agctttgagc gcgtactgat gcccggtctc gaaaagaatc
     2461 agtacagcat cctgtgggtg gagcaccagg acaagggacg gcttgagctg aattttgtca
     2521 ttccgaacat ggagctacag accggaaaac gcctccagcc gtactacgac cgggcagaca
     2581 ggccgagaat tgatgcctgg cagacgctgg tgaatcacca ttacgggctg catgacccca
     2641 acgccccgga gaaccgccgg atactgacac tccctgataa cctgccggaa acgaaacagg
     2701 cgcttgcaga gagcgtcaca cgcgggatag acgctcttta tcatgtcggg gagataagag
     2761 gccgtcagga tgtgattcag gcgctcacag aggcagggct tgaagtggtc agggtgacgc
     2821 gaagcagtat cagcattgca gacccgaacg gcgggaagaa tatcaggctg aaaggagcat
     2881 tttatgagca atcttttaca gacgggcgtg gagttcgaga aaaagctgaa agagagagcc
     2941 gaatctacag agaaaatgct gaacgacgaa ttcaggaagc tcggcgaatc tgtaagcgag
     3001 gctgtgacct caaacgagac gaaaatcaaa ggcgctatag cccagtttac ggcctcgacg
     3061 gaggaatcgc tgaaaaaaca ccgggaaggg gtgaaagagg cgatgatgca gcaccggaag
     3121 gacatgttaa agctggcagg gaatacgggc atgaggttgc tggggatagt ctttctcctg
     3181 tttaccgcga gtggcgggac gctctggtat cttggcgggc ggatacaggc gaacctggaa
     3241 gaaatcagga tacaggaaga gacattgcag aaactgaacg cgaagacatg gggcgtggag
     3301 tttgtgcagg acgggagaag gaaatttctg gtccttccgt acgggaaatc ggcaacggtg
     3361 attccctatc aggggaagga ctgggtacaa ctgacggagt gacacagagt gacggagctg
     3421 gaaagacatt tgctgaacgc attagagcaa ctgcaacagg actatatgca gcggctgagc
     3481 gaatgggaga gcgccttcgt ggaattgcag aaaatgtttt cgcttacgca acgggacaac
     3541 gcgatgctga acgagcaggt catgcagttg agtcagcagg tgcagcactt gagcgagcgg
     3601 acagaacgct tgagccagtt atacagcgag aacaggagat aagggacgaa cggctgatac
     3661 aggaacgcga acatgcgtta tccctggaac gggagcgtca gccggaaata caggaacgca
     3721 cgctggatgg tccttcgctg ggatggtgaa accatgaaaa atggcagctt cagtggatta
     3781 agaggggtta atgtggcctg taccatctgg ttgcataggt attcatgcgg ttaaaattta
     3841 tcaggcgcga tcgcggcagt ttttcgggtg gtttgttgcc atttttacct gtctgctgcc
     3901 gtgatcgcgc tgaacgcgtt tcagcggtgc gtacaataag ggattatggt aaatgtctcc
     3961 cttactgcct gccctcgtag ccatcgagat aaaccgcaga aatcgtgtca gccagcagct
     4021 tcattttttc cggtgatggc atatcactgt tcgtgtattt tctgcccgca aaggtggttg
     4081 tggttctcag tgactgaagg aattcttcag agtggaattc ttttacacgt tgctgtgccg
     4141 tttctgctga tactccggct gctttgtctt tcatccccat atcccggaac tgctgaaaga
     4201 acgggtgcat catacgtatg gtgtctgctt tctgggtgtg gaagttagga tcaaatccat
     4261 catctgaagc ataaacataa tgccgggtat gtcgttccag gctggtcttt ggtgtgcatc
     4321 ctgtaagagc tattgttgct gttatcagag gtatcagaag cagcctgaca gcggcagatc
     4381 tcataaacat ctccttgcgt ttttaatctt gatactcagg gagttatatc tccgaagagt
     4441 atctcttatc atacgtcggt ttactcccaa tgtgacttga tggtcaatga gtcaggaata
     4501 atcccgtgaa aaaatgtgtg ccagtcctgc tttttagtgt tctttctgct tcggttgcca
     4561 gtcatgcgga cagcaatgtt tttcaacaaa aggccgagaa gacaaaaaac ggcattgttg
     4621 cggatgttaa tgccagacag aaaatgttca gtaacagact gaagatacaa cttcagatcg
     4681 atgagttgag agcagagctg gctggcacca gagatgccca aaaaagagag gatttgcgta
     4741 aaaaactggc gtctctggag cggcaaaaga aatccttacg ctgatggggg cgtattttcc
     4801 ccggtttctt ccataacctt tttaccgttc agatatttac gggcgaggcg aatgcgtctt
     4861 gcctggtata ccgccgagta agcctgggtg ggattcacac cgttactgat ttcactgacc
     4921 acatgacgct gatcagccag tgtcagcgtg taaacctgtt tctggatgag gtcacgggct
     4981 ttgtcatgct catatccggc agcaaccagg gtgtcgagaa gtcgcttgtc tgttttgttc
     5041 agtttatggc ggggagattt gctcacatcg ttctccgttt tttcatgatc caacagtttt
     5101 atatctcatc ctgatttgct ccggtgttct gtttcgacgt tctcaatgtc tgcctcccca
     5161 tagtgtgatc cttattgcaa aaatgaaaat ccatgctctt gacatggata atgctgagta
     5221 gtaggttttt gctgaataca aaaccagtgg ttatatgtac agtatattgc ttttaattta
     5281 ttgttttaat tatcaaagag gaatttatgg ctaaagaact aagtggatat ggcccaactg
     5341 ctggtgagtc tgtgggggga acaggggcga atctgaatca gcaaggtgga aataataata
     5401 gtaatagtgg ggttcactgg ggtggtggtt caggctctgg taatggaggt ggtgaacatg
     5461 gaagtcagac ggggtgggga tggagtaaaa ccaataatcc ggatgtgccg ccttatgttg
     5521 atgataatgg gcaggtgcgc attaccatca caaatggtct ggtaaaaaca cctgtatatg
     5581 gagttcctgg ggctggcggg aatagtgatg ttcagggggg ctatattcct gaaaatccta
     5641 atgatgaagt tgctcgtaag tgggataaaa acaacttacc gcgagagatt gatgtgagta
     5701 tcgatggttt taaatatcgt gtaacgctta atgataatgg tagagctatc ggtattcttc
     5761 gaacaggtgt ccgtccttac gtagggagtg aaaaagctaa ggcgggtatt atggaaaaaa
     5821 ttaatcataa aacacctgag gagatatatg aggcgttagg ttttaataaa gatgaatctc
     5881 agcggcagga aaaagcaaaa caacaggctg aagatgcgtg ggataatttg cctcctaatg
     5941 ttagaaaatt tgatgttgat gttgaacagt tccactattt agtggtgctt gatgattatg
     6001 gtaatgtttt atctataact agaacaggtg ttcgtcctta tgtagggagt gaaaaagcta
     6061 aggctggtat tatggataaa gtcgaccata agaccccgga agagatatat gaggcattag
     6121 gatttaataa tgaagagcct caacgacaga atcaagctaa aaaagctgct tatgatgtgt
     6181 tttattcttt cagtatgaac cgtgaccgga ttcagagtga tgtacttaat aaagctgctg
     6241 aagttatctc tgatataggt aataaggtcg gtgattatct tggaaatgct tataagtcgt
     6301 tagcaagaga aattgctgat gatgtcaaaa atttccaggg gaaaaccatc cgtagctacg
     6361 atgatgcaat ggcgtcatta aataaagttt tgtctaatcc tgggtttaaa tttaacaggg
     6421 ccgacagtga tgcactggcg aacgcctggc gaagtattga tgctcaggat atggcaaata
     6481 aactgggtaa tatcagcaag gctttcaaat ttgccgatgt ggtgatgaag gttgagaagg
     6541 tccgggagaa aagcatcgag ggctatgaaa ccgggaactg gggacctctg atgctggagg
     6601 tggagtcctg ggtgctcagt ggcattgctt ctgctgtggc cctgggggtt ttttcagcca
     6661 cattaggggc atatgcctta tctcttgggg ctcctgctat tgctgtcggt atcgtaggta
     6721 ttctactcgc agcagttgta ggtgcattaa ttgatgacaa gtttgcagat gcactaaata
     6781 acgaaatcat taaacccgct cattaataaa taccgggtaa aaggaaggca gggttactgc
     6841 ctttctttta ataatttaaa cgtggtaact ggtgtgaata gcgtgaaata gcttataaaa
     6901 aaaatgatta agtacaggcc aatataaaat
//
