LOCUS       CP057498                2590 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB24-C10 plasmid pRHB24-C10_6, complete
            sequence.
ACCESSION   CP057498
VERSION     CP057498.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148227
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH15 T2-C10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 152.511299710597x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 09:11:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,897
            CDSs (total)                      :: 4,776
            Genes (coding)                    :: 4,664
            CDSs (with protein)               :: 4,664
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 54 of 112
            Pseudo Genes (incomplete)         :: 44 of 112
            Pseudo Genes (internal stop)      :: 28 of 112
            Pseudo Genes (multiple problems)  :: 14 of 112
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB24-C10"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB24-C10_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(366..1355)
                     /locus_tag="HVY10_24475"
     CDS             complement(366..1355)
                     /locus_tag="HVY10_24475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="QMI30950.1"
                     /translation="MQNPKLQYLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYEHYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGEMLNRMNAAFQRMKVRKEFLPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMAPPGMLNGKSYVRHERWVELWRECLRVDYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESD"
                     /name="protein rep"
     Typing          complement(2..154)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.484"
                     /coverage="89.0"
                     /evalue="1.02e-62"
     Typing          complement(280..363)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.941"
                     /coverage="93.0"
                     /evalue="1.22e-26"
     Typing          1928..2009
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.976"
                     /coverage="93.0"
                     /evalue="3.4000000000000004e-27"
     Typing          complement(2489..2590)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.204"
                     /coverage="89.0"
                     /evalue="2.28e-34"
     Typing          449..640
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.446"
                     /coverage="100.0"
                     /evalue="2.86e-91"
ORIGIN
        1 acccctacaa agcggtgtcg gcgcagctac aacaccgcgc cgacaccgct tttttaaata
       61 tcacaaagag agcaagagaa actaatttgt cataactctc tatttataaa gaaaaatcag
      121 caaaaaattg tttttgcgtg gggtgtgggc atttcaggca tgagaacaga ctcgcgtttt
      181 tccggagtat cagcggaatt cttgattaag aaacactcga cgcagagcga aattttatgc
      241 aacagcggct gtactgcaca gggattattt atcagttgaa acaggacagg atgtgtaatt
      301 gtaataccgt cacacgcgac gctatcacaa ttaccacctg gtcagggctg cgccccgaca
      361 ccctgctagt ctgattcagt tttatctttc gtcggtgagc ggcggtactt tctgacctcg
      421 gttttccatt cgaacgcgat gcgtgaaccg tcatcgtcgc cctcggaaat atcatctccg
      481 atcaccatgt cggcgtcggt ttcctgatcg agtttcagca catctttgag tgcgccgccg
      541 gtcgccacaa agcggcgctt gtgcgtctgg cgagtcagtt cgaggaacca ttcaggatcg
      601 gccaccatgt cagccggctt cgtggaatat ttcagggttt cggccactgc gccgcgcacc
      661 agttcagcgg tcgcgcaggc taaggactcg ccatccttcg gcttgcgggg tttcactgcc
      721 cggacatcaa cattcgggtc ataatcaacc cgcaggcact cccgccatag ctcaacccag
      781 cgttcatgcc tgacgtaact tttcccattt agcatgccag gaggagccat catcaaggta
      841 tggaagtgcg ggtgtgcgct gccgtcgctg ccgcgggtaa cttccgttgt gcggatccag
      901 ccctgaacag gcaggaactc cttgcgaacc ttcatacgct gaaaagctgc attcatccgg
      961 ttcagcatct cgcccagttc cccaatcgca cagttgcgca cggtcagggt caggaacatc
     1021 cagcgggcat cgggataatc cgccacgata cgcggcaggg actggtagaa acgggcttgc
     1081 cacatcaggg agcgcctcca ctggcagaca ggacagtgcc ggacacggca gaaatgtgcc
     1141 tcacgcagcc tcagacgcgt ttctccggtt tccttgaggg tagaccaccc gaaacgcagt
     1201 aaaccgccac aggaagccat acgagccccg tagtgctcat attcggccgc aagcaggtaa
     1261 atccctccca cgtcgtcaga aacgcttctg tgtgcgtccc agggcgcatc agagggggaa
     1321 taatcagtga ggtactggag tttagggttt tgcatgcaca ccgccccata ctgggcgatg
     1381 gctcgattga cgtgttatca ttgccatatc acctgttgct ctcatcatga atccagtttc
     1441 tgcaggtggt cacacccccg aacgatgccc gtcgttgcgg gggtcacttt taacgcaacc
     1501 actcccgaca gaataactcc aaaaccattt tccggcaaat cccccctcag gccttgcggc
     1561 acaagggctg gaggcgaatt tgaatcctag agttatccct taagtataaa tacaaggatc
     1621 acgcgcgaga tttgcttgaa ttttgagctg gattttcagt aaccaaaaac ctcccaaccc
     1681 cacccactta cacccatctc atccacagac tttcaccccg attttgtgaa caacaaaaaa
     1741 ccgtcttgca ggtggttggc ctcactccgt gacaccaacc accatcccca gtgttttccc
     1801 cgtgccgtcg tcaatcacaa aatcggaacc cacgccagag gcgcggggcc ccgcgatttt
     1861 ctgtttgacg agttcgggaa cggtgagcgg tacgcggaat tttttcgcgg gccgtgaggc
     1921 gttggcgggg tgtcggggcg cagccctgac caggtggtaa ttgtgatagc gtcgcgtgtg
     1981 acggtattac aattacacat cctgtcccga aaattcccgc cgtttctcgg ttttttgagc
     2041 gtcgcagacc gcacgacaaa cgaaacgaag tgaagtttgt ataagtgcgc acttcgtgag
     2101 attccctgac tgccagtttg tcgatcacgc ggaacggctg cggcacaaaa acggcaaaat
     2161 gatttgctgt ccggtcgcca gaatttggcg cgtcggtggc gatggctgta cggaacggga
     2221 acgaccgcgg ggtggggcgc gtggtgtaag gacttgttac gcgcaccgct aaaacgcgct
     2281 gaacgcgatt ctgccgcgaa caccgcaaaa agggatgttt tccccagggt aaaccggaaa
     2341 aatgcgtcag gaacgttttc agcgcgttgc atgactatgc atgaaactga atggcgatcg
     2401 gtttgggcgc atacagtgcc catgagacat attttcggac attttcaatt cgtgaatccc
     2461 tgactcattg ctacatatct gaacagacac ccctagcagc gcccccaggg gcgtcctgta
     2521 aaaaaacaca ccgcgccgct agcagcaccc ctaatataaa attatgtttt ttatgaaaat
     2581 agtcagtacc
//
