LOCUS       CP057497                5147 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB24-C10 plasmid pRHB24-C10_5, complete
            sequence.
ACCESSION   CP057497
VERSION     CP057497.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148227
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 5147)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH15 T2-C10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 152.511299710597x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 09:11:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,897
            CDSs (total)                      :: 4,776
            Genes (coding)                    :: 4,664
            CDSs (with protein)               :: 4,664
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 54 of 112
            Pseudo Genes (incomplete)         :: 44 of 112
            Pseudo Genes (internal stop)      :: 28 of 112
            Pseudo Genes (multiple problems)  :: 14 of 112
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB24-C10"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB24-C10_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(5039..5147,1..101)
                     /locus_tag="HVY10_24450"
     CDS             join(5039..5147,1..101)
                     /locus_tag="HVY10_24450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMI30945.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLRKLPPDHTIPPDLTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            complement(167..700)
                     /locus_tag="HVY10_24455"
     CDS             complement(167..700)
                     /locus_tag="HVY10_24455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMI30946.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISLDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVTLED
                     KLAEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(703..1629)
                     /locus_tag="HVY10_24460"
     CDS             complement(703..1629)
                     /locus_tag="HVY10_24460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QMI30947.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPDRGGAKKDSGNLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYYRHLEQARMVGHDIREWLDTPVKQHELVRRLQHDIAREHGFD
                     MRDGVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1806..2204
                     /locus_tag="HVY10_24465"
     CDS             1806..2204
                     /locus_tag="HVY10_24465"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMI30948.1"
                     /translation="MRISNIEWLKKRIEFIRKLGKQTARQRQIIDLLDNEDRLTEQERK
                     LLHVLATAEKNDLQAQESERKQAIQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDMLLQRLKPIKYDLDTCPNPDA"
                     /name="hypothetical protein"
     gene            complement(2397..3305)
                     /locus_tag="HVY10_24470"
     CDS             complement(2397..3305)
                     /locus_tag="HVY10_24470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QMI30949.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPEFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGRSIAKYTHRKFSPEGFSAVQAARGRKGGIAKGEAYEDKRFMALCMLENGYSQ
                     KAIAAMLNVHRNTIRNWAMHK"
                     /name="replication initiation protein"
     Typing          complement(2578..3305)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.473"
                     /coverage="88.0"
                     /evalue="0.0"
     Typing          complement(707..1629)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caataccgcc agatcttact gatgagcacg taccgttata tatacgaagg ctggaatact
       61 atcgactgct ttatcgacca aagcagacag aatcatattg aaaaaaacgg tgaacggttc
      121 gcgaaatcct ttcgcgctcc gtgaggcgtt ggacaagcga agcgcgtcag tttttccggc
      181 tcttacccgt gacaggtttc tccgccagtt tatcttccag tgtcacaact ttctgataca
      241 ccgccgctgt ggcgttgtag ttcctgtcga gcattgtttt cattccccat ccgagcacac
      301 agaacataac cagaaaagcc actgacacac cacccagcca tttccagcta taccctttaa
      361 gggtgttgta ttcctgcacg atattgctgt gaattgtcgc aatatccctg ttcagggcat
      421 ccgcgtttgc cttcatgttt gcgactgact cttccagccg ctgaaaatcc aggctgatgc
      481 gctcacctat cagccctgct acgtccagct tctgcacctc atttctgact tcatccctga
      541 cctgttttgc cacaccgtca gaaatatccc gatatccgcg aagcgtttcc cttgccacag
      601 ccagagaatc agcaacagct ttctgttgct ccattgcggt atgttgcaga tgctccaccg
      661 ataacaggag cgtgagtaat ttttcttctg tgctggccat gttcagaatc ccatagaaaa
      721 tccacgtgac ggtgatttaa cccgttcagg ttcagttcgg ttctgtctgc tcactccgtc
      781 tctcatgtca aagccatgtt cccgcgcaat atcatgttgc agccttctga ccagttcatg
      841 ttgcttaacc ggtgtatcca gccattccct gatgtcatgc ccgaccattc gcgcctgttc
      901 aagatggcgg taatagtccg gttcagaccg tttttgcatg atgtcatgac gctgggcatc
      961 atcaagtcgt ccggcagctt caaaacccag atgcacttcg ggacgtcgtt caatgccctg
     1021 ttctttcagc gtccggcagt caatgaagcc cctttcaaag ccatgctttc tcatatgttc
     1081 gttgtgcttc acctcccagc gttttcgcaa ttcccgcaac tgctcttttt gctgagtggg
     1141 ggtcaggttt ccggaatcct ttttcgcacc tccccggtcg ggatacctgg cgttatagcg
     1201 ccggaaatac tgttccggtg tacgttcaat gccatcattc acacgctgac tcatcatgat
     1261 atgtgcatga ggctgttcgc cgccgtcaat gctcgctttc ggattgtgaa tggcaaaact
     1321 ccaggcgtgc cgttcgccag cttcctgacg tacaaaatcc tgcaccagtt caaggcgctg
     1381 ttccggtgtc agttcacggg gtaatgcaat ttcaagttcc ctataggtcg aaccgttcgc
     1441 acgttcaaac tggtcagccg cctgccagaa atgggaagga ttatccctgg cccattccgg
     1501 catgtttccg tgtgcggaat attccaaatc ttgacggtcg cggtatttct ctttgcgctc
     1561 gatgtaatcg gcatggttgg cagcctgtcc cttgccacca aatttaacgc tgaggtgata
     1621 ggtcgccacg gtttcagccc tccgcaggag cgcactgttt ctcaacgaag gagagaaaca
     1681 tataagtgcg cttccttcta atttttcgaa ttagtacgga aggtaacccc tgcacccacc
     1741 gaccgcaagc gatatactgc acgaccacaa cacaccgcaa aaaaacacca gggagcacca
     1801 ccaacatgcg catcagcaac atcgaatggc tgaagaaacg gatcgagttc atcaggaagc
     1861 tcggaaaaca gaccgcacga cagagacaga tcattgacct gctcgacaac gaggacagac
     1921 tcacagaaca ggaacgcaaa cttctccacg tactagcaac agccgaaaaa aacgacttac
     1981 aggcacagga gagcgaacga aaacaggcga tccagaaacg aatcgagggg aagaaacaac
     2041 gacgggaacg caatcaccgg ctctttctcg cggcaggact actgatcgaa gcaggactgg
     2101 tggacacaaa aacaggcgag ctatgctata aaaaagacat gttattacaa agacttaaac
     2161 ccattaagta cgatctagac acatgcccaa acccagacgc gtaagtgttc ccgttcacgt
     2221 tccggtgccc tggtgcttcc tcgctcattc gctcgctacg ctcggtcatg agactgcggc
     2281 gagcactacc acctcaatca agcgaaaagc cgcaccattg ctgggctgac aagcagaaac
     2341 cacccaaaaa atagctgctc aaaaaaccgc cggaaaggcg ctgttatctg ataaggctat
     2401 ttgtgcattg cccaattacg gatcgtatta cgatgtacat tcagcatcgc cgcaatagct
     2461 ttctgagaat atccattctc cagcatacat aacgccatga aacgcttgtc ctcgtaggct
     2521 tcgcctttag cgatcccccc cttgcgaccg cgtgcggcct gtacagcgga aaacccttct
     2581 ggcgagaatt ttctatgcgt gtatttcgca atgctcctgc caatagcccg gcattcagct
     2641 ggtgaaagcg gaacaggaag cgatgcgttg tacatttcga cacgctgaat cacggcatca
     2701 agccactgtg agaattcagg ccagccctga cgaatcgccc tgtaagccca tttgcgcgtc
     2761 atttcgaaca ggtggcagtt tcgccccatc ccgtaatgtt tatcgatgct acggcgcgct
     2821 gaggcgctca aatcgagata atcagccagt tcatcgaggg tataggcttc ctcgcgccac
     2881 tccatcacca gccattccag gtggaacgga tttttgcaaa tcaggccgct gtagttcaca
     2941 tccgcacaaa gtttctcaca cagcgaacgt tcaaccgctg cggcatattt cagcgcctta
     3001 accgaggcat caggcgcggt tctcacggcg atgttgagtg cgtagagcag gtgagcgtgt
     3061 ccgttcacag gatttttaac ggtgatgttc ggcgcgggag cgttccggtc gctccagtca
     3121 atcgcagccc cctcgcggtc aacgtcaaaa accagccaga actgtgcatg aggctggttc
     3181 tgctggatat accgtgcaag aagcgcgcgc cctttgccgg agatacgaag gccgaaagcg
     3241 agatcatccg tgtgataggg acggtggggt aaattttcgt cgaagtattg aagcgcggcg
     3301 ctcatgtgtt acgattcctt ccaggttatc gaccgacatc gcccctgtat tttcgtgaaa
     3361 gtcttggcgg atagacacga atttcagggg cgtttgtttt tcagtcgctt acactacccc
     3421 caacgacaaa acctgtcgag ccttcccgca aatccgatcc atttaccata actcccgttg
     3481 tacgcacccc ataaaacgcc ctcaatcgct ttctgacgca aaaaacgcaa aaagacaagg
     3541 aaacaccagg ctgaaccaga aaaatgcgtc agaatcgttt tcagggcgtt gcataactat
     3601 gcacgaaagc gatgtgcgat cggtttcgtg cgttctgaac accgaacggc tcaaaacgac
     3661 aagttttctc ccctggtgcg tggcgctctg acggggtttc ctgtttccaa acttcacatc
     3721 tcgccaggac aaaaaaaacc gacctggggt cggtggcaag aatcagagtc tatgggaaat
     3781 attttagtta tcggatgctc aatattcaca aacgcaaaaa atgcctcagt gatgccctaa
     3841 aacaaccttt ccagaaggag tccgcgcttt acgatgcacg gaagagataa aaaaccccac
     3901 aaaagtgggg aaccaagaga aacaacaatt acaggttgtt ggttgtgttc gaacaacgat
     3961 gaagtagcta cgctaaataa taattaccgt acaggcccta catattcaac actaaattta
     4021 agatcctgac gaaggattta taaatgcacg aaaacacata gacagacaaa ttcactaaat
     4081 tcatacacaa aaacaagtgt agttttctaa gtaaattaga ttcgacaacc aattagttta
     4141 tccacatcgc attccactct tgctacacca atcacatttc ttgatatgta atagcagaaa
     4201 aaaataaaac cagacaccaa tagaccaaaa aacaaacatt caacaagaaa cattttgaac
     4261 aatatcacaa aactgtcaca accatcatca gactattgaa aaaaactggt tgctgtttta
     4321 taaaagcgac tgtaacaacc tccactctat agttccatta gtttaaagtg tcacacaaac
     4381 tgagcatcca tttgttttcc ttacttttgc ccctgtgttc caaggggttt ttttataact
     4441 taaagattag cacaccacac aaaaaacaat aaaaaacaac aagttataca ataaaaacag
     4501 tactatccat acagctaatt acatataaat accattaaac catctggaca ttaaaaataa
     4561 actcttataa cataaaatta atgcggttcc ggtaccatat gtcagggata atgtgcaaat
     4621 ggaggtatcg gaactgcatt tcagttcatt attaattaat gacaaaaaaa accctctgag
     4681 agcgcagagg gggtaaggaa tgttcccggt cataatatgt cgatagtgtt tatatacata
     4741 cagaacaaga acaacaacca accaactaat aaatatttta ttttttagag ctgaaacctt
     4801 aaagctcact tccattccat acattgttat aaaccttatg ttgaaaactg aaagtaagta
     4861 cggcagactt attaagttgc gtgcagtctt ttcacgttat acaaacaaga acacctgcga
     4921 agttatttct ccttacatat ccccctatgc caacggggga ttttttctgg tttactaacg
     4981 cataaataat agtgaccccc tatcattcac gaaccgtgat aatataagga ttatcatcat
     5041 ggcaaaatta ggtgaaaaca ccccacatat tatcgatgct actgtagatt ttatggcatc
     5101 aagccaggca tttcgtgagt atctgagaaa acttccccct gatcaca
//
