LOCUS       CP057496                6229 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB24-C10 plasmid pRHB24-C10_4, complete
            sequence.
ACCESSION   CP057496
VERSION     CP057496.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148227
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6229)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH15 T2-C10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 152.511299710597x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 09:11:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,897
            CDSs (total)                      :: 4,776
            Genes (coding)                    :: 4,664
            CDSs (with protein)               :: 4,664
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 54 of 112
            Pseudo Genes (incomplete)         :: 44 of 112
            Pseudo Genes (internal stop)      :: 28 of 112
            Pseudo Genes (multiple problems)  :: 14 of 112
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB24-C10"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB24-C10_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(4958..6229,1..456))
                     /locus_tag="HVY10_24415"
     CDS             complement(join(4958..6229,1..456))
                     /locus_tag="HVY10_24415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="QMI30938.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENNP
                     WGGGSGSGVHWGGGSGNGNGGGNGNSGGGSNSSVAAVAFGFPALATPGAGTLGIAVSGE
                     ALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVTNVQV
                     STLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASFPGVP
                     SLTVSTVKGLPVSTTLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKPIYVSV
                     TDVLTPAQVKQRQDEENRLQQEWNDTHPVEVAERNYEQARAELDQANKDVARNQERQAK
                     AVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQRAQTDVN
                     NKKAAFDAAAKDKSDADAALGAALERRKQKENKEKDSKDKLDKESKRNKPGKATGKGKP
                     VGDKWLDDAGKDSGAPIPDRIADKLRDKEFKSFDDFRKKFWEEVSKDPELSKQFKDSNK
                     TNIQKGKAPFARKKDQVGGRERFELHHDKPISQDGGVYDMDNIRVTTPKRHIDIHRGK"
                     /name="colicin"
     gene            complement(677..1210)
                     /locus_tag="HVY10_24420"
     CDS             complement(677..1210)
                     /locus_tag="HVY10_24420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMI30939.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(1213..2139)
                     /locus_tag="HVY10_24425"
     CDS             complement(1213..2139)
                     /locus_tag="HVY10_24425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QMI30940.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIEGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFETAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREYGFD
                     MRDGVSRQERTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            2316..2714
                     /locus_tag="HVY10_24430"
     CDS             2316..2714
                     /locus_tag="HVY10_24430"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMI30941.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQENERKQAVQNRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(2975..3994)
                     /locus_tag="HVY10_24435"
     CDS             complement(2975..3994)
                     /locus_tag="HVY10_24435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QMI30942.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKC"
                     /name="replication initiation protein"
     gene            complement(4490..4633)
                     /locus_tag="HVY10_24440"
     CDS             complement(4490..4633)
                     /locus_tag="HVY10_24440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QMI30943.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            complement(4695..4955)
                     /locus_tag="HVY10_24445"
     CDS             complement(4695..4955)
                     /locus_tag="HVY10_24445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="QMI30944.1"
                     /translation="MELKHSISDYTEAEFLEFVKKICRAEGATEEDDNKLVREFERLTE
                     HPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKPGFKQG"
                     /name="bacteriocin immunity protein"
     BGC             1..6229
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(3220..3994)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.263"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(1217..2139)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.403"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgacgtcaca atctttgaca tcatattcgg gtcatctttt gctatttcag atggcagaat
       61 gccgtaaagc gcaatacccc atgcgctgaa tttaaacgga cctttcaggg ctgcaaagat
      121 atcagctatt gccgcagata aagcctcacc agagacggcg atacccagcg ttccggcacc
      181 aggagttgcc aaagcaggaa aaccaaatgc cactgctgct acggatgaat tgctgccacc
      241 accggaatta ccattccccc cgccattacc attgccggag ccacctcccc agtgaacacc
      301 actaccggaa ccgccacccc atggattatt ttccgaactc catcctgaac cgtcagaagc
      361 cccaccattt ccaccaagcc ccgtagggcc gccattaatg ttaccacctg tgttatgtgc
      421 gccactgttg tggtcgcgtc catctccacc gctcataaaa attccctctt ttaagcgtta
      481 aaacaattga ttacatagtt aatgctgtac ataaaaccac tggttttatg tacagaccaa
      541 aaagtactcg ccattatttt ttctgtcaag tcaggggggg atattttttg gcagttcgga
      601 ggaatcactc atgaaaacgg tgaacggaac gcgaaatcct ttcgcgggtc gtgaggcgtt
      661 ggacaagcga agcgcgtcag tttttccggc tcttacccgt gacaggtttc tccaccagtt
      721 tatcttccag tgtcacaact ttctgataca ccgccgctgt ggcgttgtag ttcctgtcga
      781 gtattgtttt cattccccat ccgagcacac agaacataac cagaaaagcc actgacaccc
      841 cacccagcca tttccagcta taccctttaa gggtgttgta ttcctgcacg atattgctgt
      901 gaattgtcgc aatatccctg ttcagggcat ccgcgtttgc cttcatgttt gcgactgact
      961 cttccagccg ctgaaaatcc tggctgatgc gctcacctat cagtcctgcc acgtccagct
     1021 tctgcacctc atttctgact tcatccctga cctgttttgc cacaccgtca gaaatatccc
     1081 gatatccgcg aagtgtttcc cttgccacag ccagagaatc agcaacagct ttctgttgct
     1141 ccattgcggt atgttgcaga tgctccaccg ataacaggag cgtgagtaat ttttcttctg
     1201 tgctggccat gttcagaatc ccatagaaaa tccacgtgac ggtgatttaa cccgttcagg
     1261 ttcagttcgc tcctgtctgc tcactccgtc tctcatgtca aagccatact cccgcgcaac
     1321 atcatgttgc agccttctga ccagttcatg ttgcttaacc ggagtgtcca gccattccct
     1381 gatgtcatgc ccgaccgttc gcgcctgttc aagatggcgg gaatagtccg gttcagaccg
     1441 tttttgcatg atgtcatgac gctgggcatc atcaagccgt ccggcagttt caaaacccag
     1501 atgcacttca ggacgtcgtt caatgccctg ttctttcagc gtccggcagt caatgaagcc
     1561 cctttcaaag ccatgctttc tcatatgttc gttgtgcttc acctcccagc gttttcgcaa
     1621 ttcccgcaac tgctcttttt gctgagtggg agtcaggctt cctgaatcct ttttcgcacc
     1681 tccccgctcg ggatacctgg cgttatagcg ccggaaatac tgttccggtg tacgttcaat
     1741 gccatcattc acacgctgac tcatcatgat atgcgcatga ggctgttcgc ctccctcaat
     1801 gctcgctttc ggattgtgaa tggcaaaact ccatgcatgc cgttcgccag cttcctgacg
     1861 tacaaaatcc tgcaccagtt caaggcgctg ttccggtgtc agctcacggg gcagcgctat
     1921 ctccagttcc cgataagtcg aaccgtttgc acgttcaaac tggtcagccg cctgccagaa
     1981 atgtgaggga ttatctcgcg cccattccgg catgtttccg tgtgcggaat attccaaatc
     2041 ctgacggtcg cggtatttct ctttgcgctc gatgtaatcg gcgtggtttg cagcctgccc
     2101 cttgccacca aatttaacgc tgaggtgata ggttgccacg gtttcagccc tccgcaggag
     2161 cgcactgttt ctcgacgaag gagagaaaca tataagtgcg cttccttcta attttacgaa
     2221 ttagcacgga agataccccc tgcacccgcc gaccgcaagc gatatactgc acgaccacaa
     2281 cacaccgcaa aaaaacacca gggagcacca cgaacatgcg catcagcaac atcgaatggc
     2341 tgaagaaacg gatcggattc atcaggaagc tcggagaaca gaccgcacga cagagacaga
     2401 ttattgacct gctcgacaac gaagcaggac tcacagaaca ggaacgcaaa ctcctccacg
     2461 tactggcaac agcggaaaaa aacgacttac aggcacagga gaacgaacga aaacaggcgg
     2521 tccaaaatcg aatcgaggga aaaaaacaac gacgagaacg caatcaccga ctctttctcg
     2581 cggctgggct actgatcgaa gcaggactgg tagacacaaa aacaggcgag ctatgctata
     2641 aaaaagacag gatattacag gcacttaagg aaattaagta cgatctggag acatccccaa
     2701 acccagacgc gtaagtgcct ccatccgtgt tccggttccc tggtgcttcc tcgctcattc
     2761 gctcgctacg ctcggtcatg agactgcggc gagcacgacc acctcaatca tgcggtaagc
     2821 cgcaccattg ctgggctgag aggagaaaac cgccggaaag cccgctgctc aaaaagacgc
     2881 cgaaaggcgc tgttatctga tataggcttg ttcatgtccg cgaatttaac atcccattaa
     2941 caaaaaacgg ggggtagtgt tgcttctttg ttaactaaca tttaattatt tgtttcttgg
     3001 ttagccattc tccagaatta tccatccagc gtcgaatagt ccggctgcta acaccaaccg
     3061 ccactgcaat atcttccgtt gacgctccca agatatacat atcaagcgcc ctacgacgtt
     3121 tgtcgtcgtt agcagtccac cgaacagcgg cgcttttttt ccctttgtcc gactgatttt
     3181 ctgacgaatt ggccttcccg cccttgcgac cgcgtgctgc ctgaatttct ggcgtgtgcg
     3241 tatcagccac atactgtgcg aaagtttccg gcgtgaagtt cctgtgcgtg tatttcgcaa
     3301 tgctcttgcc aatagcccga cattcagcca gtgaaagcgg aacgggaagc gatgcattgt
     3361 acatttcgac acgctggatc accgcttcaa gccactgtga gaacgctggc cagccctgac
     3421 gaatcgccct gtaagcccat ttgcgcgtca tttcgaacag gtggcagttt cgccccatcc
     3481 cgtaatgttt atcgatgcta cgacgctctg aggcgctcaa atcgagataa tcagccagtt
     3541 catcgagggt ataggcttcc tcgcgccact ccatcaccag ccattccagg tggaacggat
     3601 ttttgcaaat caggccgctg tagttcacat ccgcacaaag tttctcacac agcgaacgtt
     3661 caaccgctgc ggcatatttc agcgccttaa ccgaggcatc aggcgcggtt ctcacggcga
     3721 tgttgagtgc gtagagcagg tgagcgtgtc cgttcacagg atttttaacg gtgatgttcg
     3781 gcgcgggagc gttccggtcg ctccagtcaa tcgcagcccc ctcgcggtca acgtcaaaaa
     3841 ccagccagaa ctgtgcatga ggctggttct gctggatata ccgtgcaaga agcgcgcgcc
     3901 ctttgccgga gatacgaagg ccgaaagcga gatcatccgt gtgataggga cggtggggta
     3961 aattttcttc gaagtattga agcgcggcgc tcatgtgtta cgattccttc caggttatcg
     4021 accgacatcg cccctgtatt ttcgtgaaag tcttggcgga gagacacgaa tttcaggggc
     4081 gtttgttttt cagtcgctta cactaccccc aacgacaaaa cctgtcgagc cttcccgcaa
     4141 atccgatcca tttaccataa ctcccgttgt acgcacccct taaaacgccc tcagtcgctt
     4201 tctgacgcaa aaaacgcaaa aagacaagaa aacaccaggc caaaccagaa aaacgcgcca
     4261 gaatcgtttt tagagggctg cataactatg cacgaaagcg atgcgcgatc gatttcgact
     4321 gttccgaagg gcgaacaggt caaaatgaca agttttctcg ccctgggtgg aaagttctgc
     4381 acggggattt tagctctcat ctcccgcatg gaatttttgt gacatttcaa aaaaaaagcg
     4441 catcagaatc agcctgacac gctttgtttt tgtcaaagag gatttcgggt tactgcgttg
     4501 ctaatccggt cacttcagct gttgatgacg gggatacagt cccgccctga acatcccgga
     4561 tatagtttgc ctgacatgca gccagaataa tgactgcaag aagcaataaa ataatccctg
     4621 ttattttttt cataacgact ccttgttgtg aaagaatgcc agccattcat ccataaacaa
     4681 ccggcattca tattttagcc ctgtttaaat cctggcttac cgttagcagc tcgccattct
     4741 ttaatttctt taacaatccc ttcaggacta tcttccctgt catcgcgagg ataataaatc
     4801 agatctgaac catctgggtg ctcagttaat cgctcaaact ctctcactaa tttattgtca
     4861 tcctcttcag tagcaccttc agctctacat atttttttta caaactccag aaattcagcc
     4921 tcggtataat cactaatact atgtttcaat tccattttta cttacctcta tgaatatcaa
     4981 tatgtcgctt aggtgtggtc actctgatat tatccatatc atagacacca ccatcctgac
     5041 tgattggttt atcatgatgt aattcaaagc gttccctacc acctacttgg tctttcttcc
     5101 ttgcaaaagg tgcttttccc ttttgaatgt tcgtcttatt actgtcttta aattgcttac
     5161 taagctcggg atcttttgac acttcttccc agaatttctt ccggaaatcg tcaaaacttt
     5221 taaattcttt atcacgcaac ttatcagcaa tgcgatcagg aattggcgct cctgaatctt
     5281 tacccgcatc atcaagccat ttatcaccaa ctggttttcc tttacccgtc gccttccctg
     5341 gcttattccg tttactctcc ttatctaatt tatccttaga gtccttttct ttattttcct
     5401 tctgcttacg gcgttccagc gcagcaccta acgcagcatc agcatcagac ttatcttttg
     5461 cagcagcatc aaatgcagcc ttcttattat ttacatccgt ctgtgcccgc tgggccttaa
     5521 gccctgccat ttgccacatt cggtgaccag cagccattgg ttctcgggca aatcgctcga
     5581 attgttttat ttcagccttt gcatcagcaa gagttttatt cgcagcatca agttcacttt
     5641 tacgagaatt ataaacctga acagctttag cctgtcgttc ctggtttctg gcaacatctt
     5701 tattagcctg atccagttct gcgcgggcct gttcataatt gcgttcagcc acttctacag
     5761 gatgtgtgtc attccattcc tgctggaggc gattttcctc atcctgacgt tgttttacct
     5821 gtgcaggggt aagaacatct gtcactgata cataaatcgg cttctgccca ctttctttcg
     5881 gaaaacggat caccgcttca tgtgaaccac caccaaaggt aaatcctgca ggaacggcag
     5941 tccggccttt atcctccgta ataccacggg gaagggttgt tgacaccgga aggcctttga
     6001 cagtgctgac tgtcagagaa gggacaccag gaaatgatgc gtgaaataca cctggagtac
     6061 gtgttggttt ggcattcaca acaggcacac tcataggcac acctgcaact acggcaatat
     6121 gctgtcgtgt gtccttcaca acatctgtca cacgtttcgt cacgctgaca gttgcctggt
     6181 ccagcggaag tgtactgacc tgaacattcg tcactgtttc tgccggtaa
//
