LOCUS       CP057495                6826 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB24-C10 plasmid pRHB24-C10_3, complete
            sequence.
ACCESSION   CP057495
VERSION     CP057495.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148227
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6826)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH15 T2-C10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 152.511299710597x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 09:11:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,897
            CDSs (total)                      :: 4,776
            Genes (coding)                    :: 4,664
            CDSs (with protein)               :: 4,664
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 54 of 112
            Pseudo Genes (incomplete)         :: 44 of 112
            Pseudo Genes (internal stop)      :: 28 of 112
            Pseudo Genes (multiple problems)  :: 14 of 112
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB24-C10"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB24-C10_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(6557..6826,1..909))
                     /gene="cna"
                     /locus_tag="HVY10_24360"
     CDS             complement(join(6557..6826,1..909))
                     /gene="cna"
                     /locus_tag="HVY10_24360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin colicin N"
                     /protein_id="QMI30936.1"
                     /translation="MGSNGADNAHNNAFGGGKNPGIGNTSGAGSNGSASSNRGNSNGWS
                     WSNKPHKNDGFHSDGSYHITFHGDNNSKPKPGGNSGNRGNNGDGASAKVGEITITPDNS
                     KPGRYISSNPEYSLLAKLIDAESIKGTEVYTFHTRKGQYVKVTVPDSNIDKMRVDYVNW
                     KGPKYSNKLVKRFVSQFLLFRKEEKEKNEKEKNEKEALLKASELVSGMGDKLGEYLGEK
                     YKNVAKEVAGDIKNFHGRNIRSYNEAMASLNKVLANPKMKVNKSDKDAIVNAWKQVNAQ
                     DMANKIGNLGKAFKVADLAIKVEKIREKSIEGYNTGNWGPLLLEVESWIIGGVVAGVAI
                     SLFGAVLSFLPISGLAVTVLGVIGIMTISYLSSFIDANRVSNINNIISSVIR"
                     /name="cna"
     gene            1168..1605
                     /locus_tag="HVY10_24365"
     CDS             1168..1605
                     /locus_tag="HVY10_24365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QMI30928.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGFVSYRTGQDGRR
                     EFETSELIRAYGELKQNETPERHSTGQAENPHDQPTERILRELNELKQCLTLMLEDKQA
                     QDMDRKRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            1686..2105
                     /gene="exc2"
                     /locus_tag="HVY10_24370"
     CDS             1686..2105
                     /gene="exc2"
                     /locus_tag="HVY10_24370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMI30929.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQHFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            2299..2469
                     /locus_tag="HVY10_24375"
     CDS             2299..2469
                     /locus_tag="HVY10_24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005074609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMI30930.1"
                     /translation="MSKAPIGTTASTGQKCPESGVWEVVGTPSTTAPIAKGNVMPPYNG
                     KAVTWKLTQYA"
                     /name="hypothetical protein"
     gene            complement(2825..3058)
                     /gene="mbeD"
                     /locus_tag="HVY10_24380"
     CDS             complement(2825..3058)
                     /gene="mbeD"
                     /locus_tag="HVY10_24380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMI30931.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="mbeD"
     gene            complement(3065..3583)
                     /locus_tag="HVY10_24385"
     CDS             complement(3065..3583)
                     /locus_tag="HVY10_24385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbeB"
                     /protein_id="QMI30932.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="mobilization protein MbeB"
     gene            complement(3561..4271)
                     /locus_tag="HVY10_24390"
                     /pseudo=""
     CDS             complement(3561..4271)
                     /locus_tag="HVY10_24390"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4261..4584)
                     /locus_tag="HVY10_24395"
     CDS             complement(4261..4584)
                     /locus_tag="HVY10_24395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMI30937.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGEELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4649..4840
                     /locus_tag="HVY10_24400"
     CDS             4649..4840
                     /locus_tag="HVY10_24400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMI30933.1"
                     /translation="MTKQEKTVLNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5859..5996)
                     /locus_tag="HVY10_24405"
     CDS             complement(5859..5996)
                     /locus_tag="HVY10_24405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QMI30934.1"
                     /translation="MCGKILLILFFIMTLSACQVNHIRDVKGGTVAPSSSSRLTGVEIK
                     "
                     /name="colicin release lysis protein"
     gene            6024..6548
                     /gene="cni"
                     /locus_tag="HVY10_24410"
     CDS             6024..6548
                     /gene="cni"
                     /locus_tag="HVY10_24410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_061352107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin N immunity protein"
                     /protein_id="QMI30935.1"
                     /translation="MDIKDRNKISKKISFSLLLLLSPFALIFFSYNNAPIPLLEKIIAY
                     LSLPGFHSSNNPPLSEAFNLYVHTAPLAAISLFIFTHKELELKPKSSPLRALKILTPFT
                     ILYISMIYCFLLTDTELTLSSKTFVLMSKNDLFLSFFYITLYIGIYIFTYLYLWFFIGT
                     YKLFTRGGILP"
                     /name="cni"
     Typing          complement(4927..5013)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.864"
                     /coverage="100.0"
                     /evalue="1.13e-36"
     Typing          5024..5581
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.118"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2722..4271)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.901"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aacctttata gctaaatcag caaccttaaa tgccttgcca agattaccta tcttattagc
       61 catgtcctga gcattaacct gtttccaggc attcacaatg gcatccttat cagatttgtt
      121 tactttcatc tttggatttg ctaacacttt attcagtgaa gccattgctt cattatagct
      181 acgaatatta cggccatgaa agtttttaat atcaccggca acttccttag ctacattttt
      241 atatttttct ccaagatact cgccaagttt atcccccata ccagacacaa gttcactagc
      301 ctttagcaag gcttcttttt catttttttc tttttcattt ttttcttttt cctccttcct
      361 aaataataaa aactggctca caaacctctt cacaagttta ttgctatatt tcggtccctt
      421 ccagttcaca taatcaactc tcattttatc aatattacta tctggaacag taaccttaac
      481 atactgacct tttctggtgt gaaaagtata tacctctgta cctttaattg attccgcatc
      541 aattaatttt gccaacaatg aatattcagg atttgacgaa atataacgac ctggtttcga
      601 gttgtcaggt gtgattgtta tctctccaac cttagcgctc gctccatcac cgttattacc
      661 tcgatttcca ctattccctc caggtttagg ctttgaatta ttgtccccat gaaatgtaat
      721 atgataagaa ccatcactgt ggaagccatc atttttatga ggcttattac tccatgacca
      781 tccattggaa tttcctcggt tacttgatgc actaccatta cttcctgcgc cactggtatt
      841 accaatgccc ggatttttcc ctccaccaaa agcattgtta tgtgcattat ctgcgccatt
      901 actacccata ataaatccct ctttgacttt taaaacaaac cattaaaaat aaatactgta
      961 cacataacca ctggttttat atacagttta aaattacgcc gctgcgtttt tcctgtcaac
     1021 cctgggaatt ttcgtttttg tgaaaacgat caaaaaaaca gccagccaca ccacgtgcta
     1081 ttcccgccgg ataccacaaa aaccagcaca aacattaccg ttctcagacc tcattatgtt
     1141 ttactgaaac catgagatga gacacctatg ggacactgtc actttatggc atggcacaca
     1201 ctccgtgacg cactgaaaat gacaggcaga tcgcgttcac agttttaccg tgatatgcgc
     1261 gcaggctttg tcagttaccg caccggtcaa gacggacgac gggagtttga aaccagtgaa
     1321 ctgatccggg catacggcga attaaagcag aatgagacac cagaaaggca cagtacggga
     1381 caggcagaaa atccacatga tcagccgaca gaacgcattc tccgggagct gaatgagctg
     1441 aaacaatgcc tgacgctgat gcttgaggac aaacaggcac aggatatgga tcgcaaacgc
     1501 caggaagcag aacgggaaca gctacagaat gagatagccc agctcaggca ggcactggaa
     1561 ctagaaaaga aacggggatt ctggtccagg ctgttcggtc gctgaacgct gtcagagact
     1621 gatgataaaa tagtcttcgg ataataactc accgagaata aacacttgaa ggtagggaga
     1681 cactcatgag acgtaccgga aacaaactgt gtcttatcgc catgataaca gcaacagtag
     1741 ctctcacagc ctgtacccca aagggcagcg tggaacaaca tacccggcat tacgtatatg
     1801 cttctgatga cggttttgat cctaactttt ccacccagaa ggccgacaca acacgaatga
     1861 tggtgccttt ttttcggcag ttctgggata tgggagctaa agataaagcg acaggaaaat
     1921 cacggagtga tgtgcagcaa cgcattcagc actttcacag tcaggaattt ttaaactcac
     1981 tccggggcac aacacaattt gcgggtaccg attaccgcag caaagacctc accccgaaaa
     2041 aatccaggct gctggctgac accatttcgg ctgtttatct cgatggctac gagggcaggc
     2101 agtaagttga ttttccataa gtcctttata acaaaaccaa aacttacaac gccaaaagca
     2161 tatacaactt tgttaaaaat atgaccacaa tcacatctgt gaaaaaacga cctttaagcc
     2221 cgcttgaaag ctgatttcta aaccagaaat aagtattgct acacaagcat acggataaaa
     2281 catacaagag gaatcaatat gtctaaagcc ccaattggta caacagcttc aaccggacaa
     2341 aaatgccctg aaagcggtgt ttgggaagtg gtaggaacgc cttcaactac agcaccaatt
     2401 gccaaaggca atgtaatgcc tccgtataac ggaaaagccg taacttggaa attaacccaa
     2461 tacgcttgat atctcaaaag cccccaccgg gggctttttc gttattcttg gatttaccat
     2521 aatcccttaa ttgtacgcac cgccataatg cgttcatcgc gatcacggca gcagacaggt
     2581 aaaaatggca acaaaccacc cgaaaaactg ccgcgatcgc gcctgataaa ttttaaccgt
     2641 atgaatacct atgcaaccag atggtacagg ccacattccc cccacttaat ccactgaagc
     2701 tgccattttt catggtttca ccatcccagc gaagggccat ccagcgtgcg ttcctgtatt
     2761 tccggctgac gctcccgttc cagggataac acatgttcgc gctcctgtat caaccgttcc
     2821 tctcttatct ccagttctcg ctgtataact ggctcaagcg ttctgtctgc tcgctcaagt
     2881 gctgcacctg ctgactcaac tgcatgaccc gctcgttcag catcgcgttg tcccgttgcg
     2941 taagcgaaaa catcttctgc aattccacga aggcgctctc ccattcgctc agccgctgca
     3001 tatagtcctg ttgcagctgc tctaaggcgt tcagcaaatg tgtttccagc tctgtcactc
     3061 tgtgtcactc cttcagatgt acccactctt tcccctgaaa gggaatcacc tccgctgatt
     3121 tcccgtacgg aaggacaagg aatttcctgt tcccgtcctg cacaaactcc acgccccatg
     3181 tcttcgcgtt cagtttctgc aatgtctctt cctgcttcct gatttcttcc aggttcgcct
     3241 gtatcctccc gccaagatac cagagcgtcc cgccactcgc ggtaaacagg agaaagacta
     3301 tccccagtaa catcatgccc gtattccctg ccagctttaa cacgtccctc ctgtgctgca
     3361 tcatcgcctc tttcacccct tcccggtgtt tttccagcga ttcctctgtc gaggccgtga
     3421 acagggctat agcgtctctg attttcgtct cgtttgatgt cacagcctcg cttacagatt
     3481 cgccgagcct cctgaactcg ttgttcagca ttttctctgt agattcggct ctctctttca
     3541 gctttttctc gaactccgcg cccgtctgca aaagattgct cataaaatgc tcctttcagc
     3601 ctgatattct tcccgccgtt cgggtctgca atgctgatgc tgcttcgcgt caccctgacc
     3661 acttcaagcc ccgcctcagt gagcgcctga atcacatcct gacggccttt tatctccccc
     3721 acatgataaa gagcgtctat cccgcgtgtg acgctctctg caagcgcctg tttcgtttcc
     3781 ggcaggttat cagggagagt cagtatccgg cggttctccg gggcgttggg gtcatgcagc
     3841 ccgtaatggt gattaaccag cgtctgccag gcatcaattc tcggcctgtc tgcccggtcg
     3901 tagtacggct ggaggcgttt tccgctctgt agctccatgt tcggaatgac aaaattcagc
     3961 tcaagccgtc ccttgtcctg gtgctccacc cacaggatgc tgtactgatt tttttcgaga
     4021 ccgggcatca gtacacgctc aaaactcgcc atcacttttt cacgtcctcc cggcggcagt
     4081 tccttctccg cgaacgacag aacaccggac gtgtatttct tcgcaaatgg cgtggcatcg
     4141 atgagttccc ggacttcttc cggattaccc tgaagcaccg ttgccccttc ccggttacgc
     4201 tcccttccca gcaggtaatc aaccggacca ctgccaccac cttttcccct ggcatgaaac
     4261 ttaactatca tcccgcgtcc cctgttccct gacggccaga cgcagccggc gcagctcctc
     4321 cccgatggcc atcagtgcgg ccaccacctg aacccggtca ccggaagacc actgcccgct
     4381 gttcacctta cgggctgtct gattcagatt attcccgatg gcggccagct gacgcagtaa
     4441 cggtggtgcc agtgtcggca gcctcccgga acgggcaacc ggctcaccca gacagacccg
     4501 ccgcatccat accgccagtt gtttaccctc acaacgttcc agcaaacgag catgttcatc
     4561 atcagtaacc cgtatcgtga gcatcctctc gcgtttcatc ggtatcatta cccccatgaa
     4621 cagaaatccc ccttacacgg aggcatcagt gactaaacag gaaaaaaccg tccttaacat
     4681 ggcccgcttt atcagaagcc agacattaac gctgctggag aagctcaacg aactggacgc
     4741 agatgaacag gcagatatct gtgaatcgct tcacgaccac gccgatgagc tttaccgcag
     4801 ctgcctcgca cgtttcgggg atgacggaga aaacctctga cacatgcagc tcccggagac
     4861 ggtcacagct tgtctgtgag cggatgccgg gagctgacaa gcccgtcagg gcgcgtcagc
     4921 gggttttagc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     4981 tactggctta accatgcggc attagtgcgg attgtatgaa aagtgcgcca tgccgggtgt
     5041 gaaatgccgc acagatgcgt aaggagaaaa tgcaacttcc gacgctcttc cgcttcctcg
     5101 ctcactgact cgctacgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     5161 acggtaatgc ggttatccac agaatcaggg gataacacca aaagaaacat gtgagcaaaa
     5221 aacaagaacc cagagaaggc cacgccgctg gcgtttttcc ataggctccg cccccctgac
     5281 gagcatcaca aaaaccgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     5341 taccaggcgt ttccccctgg tggctccctc gtgcgctctc ctgttccgac cctgccgctt
     5401 accggatacc tgtccgcctt tctccctttg ggaagcgtgg cgctttctca tagctcacgc
     5461 tgttggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     5521 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     5581 agacacgact taacgccact ggcagcagcc actggtaacc gaattagcag agcgagattt
     5641 atccagatgc ttacagagtt cttgaagtga tggcccaact gcggctacac tgaaaggaca
     5701 gtatttggta tctgcgctct gctgaagcca gttacctcgg ttaagcagtc cccaactgac
     5761 ttaaccttcg actaaaccgc ctccccaggc ggttttttcg tttacaggca gcagattacg
     5821 cgcagaaaaa aaggatctca agaggatcct ttgatctttt acttaatttc aacccctgtt
     5881 agtctggatg atgatgatgg agcaacagtc cctcctttca catcacggat gtgattgacc
     5941 tgacatgcac taagtgtcat gataaaaaac aagattaata gaattttccc acacataatt
     6001 tccaaccatc caaagaaagc aacatggata taaaagacag aaataagata tcaaaaaaaa
     6061 tatcattcag tcttctgctc ttactttccc cattcgcatt aatatttttc agttataata
     6121 atgcaccaat accactcctt gaaaaaatca tcgcatacct atccctacca ggatttcatt
     6181 catcaaacaa cccaccccta agcgaagcat tcaatctcta tgttcataca gcccctttag
     6241 ctgcaatcag cttattcata ttcacacaca aagaattaga gttaaaacca aagtcatcac
     6301 ctctgcgggc gctaaagata ttaactcctt tcactattct ttatatatcc atgatatact
     6361 gtttcttgct aactgacaca gaactaacct tgtcatcaaa aacatttgta ttaatgtcaa
     6421 aaaatgacct gtttttgtct ttcttctata taacactata cattgggata tatatattca
     6481 catatttata cctttggttc tttataggaa catataaact atttaccagg ggaggtatac
     6541 tcccctgaca ctcatgtcat cgaataacac tagatataat gttatttata ttcgaaactc
     6601 gatttgcatc aatgaaagat gacaagtaac taatcgtcat tattcctatt acccccaaca
     6661 cagtaacagc aagtcctgag attgggagaa aactcaacac agccccaaac aaactaatag
     6721 caactccagc aacaacgccc ccgatgatcc atgattcaac ctccagcagg agaggtcccc
     6781 agttgccagt attgtatccc tcaatgcttt tttccctaat tttctc
//
